Article
Engineering, Environmental
Gabriel Capson-Tojo, Damien J. Batstone, Maria Grassino, Tim Hulsen
Summary: This study evaluated the light attenuation in enriched purple phototrophic bacteria (PPB) cultures. The results showed that increased biomass concentrations led to higher light attenuation, and pigment concentration had a strong effect on attenuation. A proposed mathematical model allowed effective prediction of the kinetics of photoheterotrophic growth.
Article
Engineering, Environmental
Gabriel Capson-Tojo, Shengli Lin, Damien J. Batstone, Tim Hulsen
Summary: This study evaluated the impact of different dissolved oxygen concentrations on the performance of enriched purple phototrophic bacteria (PPB) cultures in treating wastewater. The results showed that the presence of oxygen suppressed photoheterotrophic growth of PPB and led to dominant chemoheterotrophy under aerobic conditions. This suggests that PPB cannot outcompete other microbes under aerobic or microaerobic conditions, despite their aerobic capabilities.
Article
Microbiology
Sarah P. Esser, Janina Rahlff, Weishu Zhao, Michael Predl, Julia Plewka, Katharina Sures, Franziska Wimmer, Janey Lee, Panagiotis S. Adam, Julia McGonigle, Victoria Turzynski, Indra Banas, Katrin Schwank, Mart Krupovic, Till L. V. Bornemann, Perla Abigail Figueroa-Gonzalez, Jessica Jarett, Thomas Rattei, Yuki Amano, Ian K. Blaby, Jan-Fang Cheng, William J. Brazelton, Chase L. Beisel, Tanja Woyke, Ying Zhang, Alexander J. Probst
Summary: CRISPR spacers in DPANN archaea can target chromosomal DNA of their episymbionts, and this phenomenon is widespread across different archaeal lineages. Using metagenomics, metatranscriptomics, and single-cell genomics, researchers found that CRISPR spacers in Candidatus Altiarchaeum crystalense and Ca. A. horonobense match putative essential genes in their episymbionts' genomes. Metabolic interaction modeling also indicates that episymbionts can be either parasitic or mutualistic depending on the host genotype. Moreover, CRISPR-Cas targeting of genomes associated with symbiotic archaea is independently evolved in various archaeal lineages, as suggested by an analysis of 7,012 archaeal genomes.
NATURE MICROBIOLOGY
(2023)
Article
Engineering, Environmental
Felix Egger, Tim Hulsen, Damien J. Batstone
Summary: Anaerobic digestion produces biogas that requires desulfurization before use due to its corrosive nature. A continuous desulfurization process using purple phototrophic bacteria, powered by light, was developed as an alternative to traditional methods. This caustic-free process can integrate with anaerobic digestion using centrate as a nutrient source, but may be limited by the availability of light for nighttime operation in a scaled process.
CHEMICAL ENGINEERING JOURNAL
(2023)
Review
Microbiology
Marleen van Wolferen, Andre Arashiro Pulschen, Buzz Baum, Simonetta Gribaldo, Sonja-Verena Albers
Summary: This review explores archaeal morphology, internal organization, and cell division, and discusses their importance in ecology, biotechnology, and human health. Recent studies have revealed the diversity and ubiquity of archaea in nature, expanding the phylogenetic scope of archaea and shedding light on their role in the evolutionary origins of eukaryotes. Advances in culturing and live imaging techniques have contributed to a better understanding of archaeal biology. The review also highlights technical challenges and discusses the potential of new technologies to address key unanswered questions.
NATURE MICROBIOLOGY
(2022)
Article
Engineering, Environmental
Md Abu Hanifa Jannat, Jangwoo Lee, Seung Gu Shin, Seokhwan Hwang
Summary: The study enriched propionate degrading consortia from four different seeding sources and identified dominant syntrophic microbial groups through 16s rRNA gene based metagenomics analysis. Accelerated microbial shifts were observed at high-strength phase with a significant decrease of Syntrophaceae up to 26.9%. Response Surface Methodology revealed that pH 6.8-6.9 and temperature 34.5-34.9 C-omicron were optimum growth conditions for the propionate degradation culture.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Engineering, Environmental
Cristian A. Sepulveda-Munoz, Andres F. Torres-Franco, Ignacio de Godos, Raul Munoz
Summary: Purple phototrophic bacteria (PPB) are efficient in nutrient and carbon removal from piggery wastewater, especially under the condition of UV-VIS filtered photosynthetic active radiation. Temperature and the type of inoculum also influence the performance of PPB in wastewater treatment.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Engineering, Environmental
Cristian A. Sepulveda-Munoz, Gorka Hontiyuelo, Saul Blanco, Andres F. Torres-Franco, Raul Munoz
Summary: Photosynthetic processes can be used to treat wastewater and convert nutrients into biomass. In this study, a combination of purple phototrophic bacteria photobioreactor and microalgae-bacteria photobioreactor was used to treat piggery wastewater, achieving efficient removal of carbon and nitrogen contaminants.
JOURNAL OF WATER PROCESS ENGINEERING
(2022)
Article
Microbiology
Francesco Di Nezio, Clarisse Beney, Samuele Roman, Francesco Danza, Antoine Buetti-Dinh, Mauro Tonolla, Nicola Storelli
Summary: Meromictic lakes provide a stable anoxic environment for anaerobic microorganisms, with Lake Cadagno being a habitat for phototrophic sulfur bacteria contributing significantly to the lake's total productivity. Different populations of these bacteria display varying abilities in CO2 fixation through photosynthesis and chemosynthesis, showing substantial differences in activity and abundance.
FEMS MICROBIOLOGY ECOLOGY
(2021)
Article
Microbiology
Gabriela F. Paredes, Tobias Viehboeck, Raymond Lee, Marton Palatinszky, Michaela A. Mausz, Siegfried Reipert, Arno Schintlmeister, Andreas Maier, Jean-Marie Volland, Claudia Hirschfeld, Michael Wagner, David Berry, Stephanie Markert, Silvia Bulgheresi, Lena Koenig
Summary: The study investigated the effect of oxygen on Candidatus Thiosymbion oneisti, a gammaproteobacterium living on marine nematodes. Surprisingly, sulfur oxidation genes were upregulated under anoxic relative to oxic conditions. In addition, stress-related genes were upregulated under oxic conditions, suggesting the symbiont's response to oxygen availability.
Article
Biotechnology & Applied Microbiology
Fabienne Hilgers, Fabian Hogenkamp, Oliver Klaus, Luzie Kruse, Anita Loeschcke, Claus Bier, Dennis Binder, Karl-Erich Jaeger, Joerg Pietruszka, Thomas Drepper
Summary: In this study, we successfully implemented a light-mediated on-switch for target gene expression in the facultative anoxygenic phototroph Rhodobacter capsulatus using photocaged IPTG. We also demonstrated its application in inducing intrinsic carotenoid biosynthesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Johannes F. Imhoff, Terrance E. Meyer, John A. Kyndt
Summary: The purple sulfur bacterium Thiospirillum jenense DSM216(T) has a genome size of 3.22 Mb and belongs to the Chromatiaceae family. It possesses sulfur oxidation pathway and a photosynthetic gene cluster, but lacks parts of the tricarboxylic acid cycle and is highly sensitive to oxygen.
ARCHIVES OF MICROBIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Olga Senko, Olga Maslova, Aysel Aslanli, Elena Efremenko
Summary: The results of this study are important for the development of new biocatalytic approaches to treat complex wastewater contaminated by perfluorocarbons and organophosphorus compounds. The study also showed the potential for simultaneous production of phototrophic microorganisms as renewable raw materials and predicting changes in aquatic ecosystems. The investigation found that the presence of perfluorocarbons significantly increased the biomass accumulation rate for Chlorella vulgaris cells, and a consortium of microalgae and bacterial cells achieved the highest biomass yield.
APPLIED SCIENCES-BASEL
(2023)
Review
Biotechnology & Applied Microbiology
Kartik Dhar, Kadiyala Venkateswarlu, Mallavarapu Megharaj
Summary: The metabolic flexibility of anoxygenic phototrophic purple non-sulfur bacteria (PNSB) has been utilized in various biotechnological applications, including wastewater treatment and bioremediation of heavy metals and organic pollutants. However, the potential role of PNSB in the biodegradation of toxic organic pollutants remains largely unexplored, calling for further research.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Victor Fernandez-Juarez, Soren Hallstrom, Cesar O. Pacherres, Jiaqi Wang, Guillem Coll-Garcia, Michael Kuhl, Lasse Riemann
Summary: This study examined the factors that regulate nitrogen fixation in an anoxygenic phototrophic bacterium and found that light and anoxia stimulated nitrogen fixation. The study also revealed the importance of biofilm formation for nitrogen fixation under oxic conditions. These findings highlight the physiological adaptations and the role of light in marine non-cyanobacterial diazotrophs.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2023)