期刊
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
卷 4, 期 -, 页码 -出版社
FRONTIERS RESEARCH FOUNDATION
DOI: 10.3389/fcimb.2014.00109
关键词
Streptococcus pyogenes; group A streptococcus; phage-like chromosomal islands; SpyCl; mutator phenotype
资金
- Oklahoma Center for the Advancement of Science and Technology (OCAST) [HR11-133]
- NIH [R15A1072718]
Streptococcus pyogenes is a significant pathogen of humans, annually causing over 700,000,000 infections and 500,000 deaths. Virulence in S. pyogenes is closely linked to mobile genetic elements like phages and chromosomal islands (Cl). S. pyogenes phage-like chromosomal islands (SpyCl) confer a complex mutator phenotype on their host. SpyCl integrate into the 5' end of DNA mismatch repair (MMR) gene mutL, which also disrupts downstream operon genes ImrP, ruvA, and tag. During early logarithmic growth, SpyCl excise from the bacterial chromosome and replicate as episomes, relieving the mutator phenotype. As growth slows and the cells enter stationary phase, SpyCl reintegrate into the chromosome, again silencing the MMR operon. This system creates a unique growth-dependent and reversible mutator phenotype. Additional Cl using the identical attachment site in mutL have been identified in related species, including Streptococcus dysgalactiae subsp. equisimilis, Streptococcus anginosus, Streptococcus intermedius, Streptococcus parauberis, and Streptococcus canis. These Cl have small genomes, which range from 13 to 20 kB, conserved integrase and DNA replication genes, and no identifiable genes encoding capsid proteins. SpyCl may employ a helper phage for packaging and dissemination in a fashion similar to the Staphylococcus aureus pathogenicity islands (SaPI). Outside of the core replication and integration genes, SpyCl and related Cl show considerable diversity with the presence of many indels that may contribute to the host cell phenotype or fitness. SpyCl are a subset of a larger family of streptococcal Cl who potentially regulate the expression of other host genes. The biological and phylogenetic analysis of streptococcal chromosomal islands provides important clues as to how these chromosomal islands help S. pyogenes and other streptococcal species persist in human populations in spite of antibiotic therapy and immune challenges.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据