Article
Multidisciplinary Sciences
Jonathan Markert, Keda Zhou, Karolin Luger
Summary: The study demonstrates that SMARCAD1 is capable of transferring the entire histone octamer from one DNA segment to another in an ATP-dependent manner, as well as de novo nucleosome assembly from histone octamer due to its ability to simultaneously bind all histones. The low-resolution cryo-electron microscopy structure of SMARCAD1 in complex with a nucleosome reveals a unique engagement of its adenosine triphosphatase domains with the substrate, providing mechanistic insights into nucleosome disassembly and reassembly induced by SMARCAD1.
Review
Biochemistry & Molecular Biology
Celine Duc, Christophe Thiriet
Summary: The review focuses on the remodeling of chromatin during DNA replication in the S-phase, including the eviction of nucleosomes and the assembly of newly synthesized histones. The recycling of parental histones and the nuclear import of histones are also discussed in relation to epigenetic inheritance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Pengfei Guo, Nam Hoang, Joseph Sanchez, Elaine H. Zhang, Keshari Rajawasam, Kristiana Trinidad, Hong Sun, Hui Zhang
Summary: The authors demonstrate that the assembly/disassembly of mammalian SWI/SNF complexes is dynamically regulated by a lysine methylation mechanism involving LSD1 demethylase and L3MBTL3 methyl-lysine reader protein. This mechanism targets SET7-methylated lysines in SMARCC1 and SMARCC2 for proteolysis by CRL4 ubiquitin ligase. The study also reveals the critical role of LSD1 and L3MBTL3 in maintaining the pluripotency and self-renewal of embryonic stem cells, as well as regulating proteolysis induced by loss of SWI/SNF subunits.
NATURE COMMUNICATIONS
(2022)
Article
Cardiac & Cardiovascular Systems
Raja Chakraborty, Allison C. Ostriker, Yi Xie, Jui M. Dave, Ana Gamez-Mendez, Payel Chatterjee, Yaw Abu, Jake Valentine, Kimberly Lezon-Geyda, Daniel M. Greif, Vincent P. Schulz, Patrick G. Gallagher, William C. Sessa, John Hwa, Kathleen A. Martin
Summary: This study reveals that p300 and CBP play distinct and opposing roles in VSMC phenotypic switching and co-regulate chromatin modifications through different functional interactions with TET2 or HDACs. Targeting specific histone acetyl-transferases may hold therapeutic promise for cardiovascular diseases.
Article
Multidisciplinary Sciences
Xi Lu, Naga Prathyusha Maturi, Malin Jarvius, Irem Yildirim, Yonglong Dang, Linxuan Zhao, Yuan Xie, E-Jean Tan, Pengwei Xing, Rolf Larsson, Marten Fryknas, Lene Uhrbom, Xingqi Chen
Summary: By comparing the chromatin accessibility landscape of glioblastoma stem cell (GSC) cultures from mice and patients, the authors suggest that the epigenome of GSCs is regulated by cell lineage and could predict patient survival.
NATURE COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Avital Sarusi Portuguez, Ivana Grbesa, Moran Tal, Rachel Deitch, Dana Raz, Limor Kliker, Ran Weismann, Michal Schwartz, Olga Loza, Leslie Cohen, Libi Marchenkov-Flam, Myong-Hee Sung, Tommy Kaplan, Ofir Hakim
Summary: The glucocorticoid receptor (GR), a transcription factor, regulates gene activation or repression through the spatial association of genes with multiple binding sites. We found that the chromatin interaction networks of GR-activated and repressed genes are spatially separated and vary in their regulatory elements.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Aditi Singh, Xyrus X. Maurer-Alcala, Therese Solberg, Lilia Haeussermann, Silvan Gisler, Michael Ignarski, Estienne C. Swart, Mariusz Nowacki
Summary: Recruiting the chromatin remodeler ISWI1 facilitates IES recognition and precise excision in Paramecium, ensuring efficient DNA elimination.
Article
Biochemistry & Molecular Biology
Wei Fu, Yaoguang Yu, Jie Shu, Zewang Yu, Yixiong Zhong, Tao Zhu, Zhihao Zhang, Zhenwei Liang, Yuhai Cui, Chen Chen, Chenlong Li
Summary: Three Arabidopsis SWI/SNF chromatin remodeling complexes, BAS, SAS, and MAS, have unique and shared roles, distinct organization, genomic targeting, and assembly.
Article
Biology
Elizabeth T. Wiles, Colleen C. Mumford, Kevin J. McNaught, Hideki Tanizawa, Eric U. Selker
Summary: Establishing and maintaining appropriate gene repression is crucial for multicellular organisms. H3K27 methylation is a common modification associated with gene repression, and recent research has identified the involvement of ISWI and ACF1 genes in the repression of H3K27-methylated genes in the filamentous fungus Neurospora crassa. These genes form an ACF chromatin-remodeling complex and interact with chromatin throughout the genome. Proper nucleosome positioning is critical for the repression of H3K27-methylated genes.
Article
Immunology
Ana C. F. Ferreira, Aydan C. H. Szeto, Paula A. Clark, Alastair Crisp, Patrycja Kozik, Helen E. Jolin, Andrew N. J. McKenzie
Summary: The study found that ADNP plays an indispensable role in immune reactions to allergens by recruiting proteins such as CHD4 and BRG1, serving as a critical bridge in gene activation and promoting T cell differentiation. The absence of ADNP impairs the ability of GATA3 and AP-1 to initiate histone acetylation and DNA accessibility, resulting in impaired expression of type 2 cytokines. These results demonstrate the importance of ADNP in promoting immune cell specialization.
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact through Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of-function CTCF mutation in endometrial tumors indicates its importance in uterine homeostasis. Our study reveals that uterine Ctcf plays a key role in progesterone-dependent expression of uterine genes underlying optimal post-pubertal uterine development.
Article
Biochemistry & Molecular Biology
Sylvia C. Hewitt, Artiom Gruzdev, Cynthia J. Willson, San-Pin Wu, John P. Lydon, Niels Galjart, Francesco J. DeMayo
Summary: Receptors for estrogen and progesterone interact via Cohesin/CTCF loop extrusion at enhancers distal from regulated genes. Loss-of- function CTCF mutation indicates its importance in uterine homeostasis. Deleting Ctcf gene in female reproductive tissues of mice resulted in reduced uterine glands and hypoplastic uteri with decreased endometrial stroma. Ctcf deletion also altered the expression of progesterone-responsive genes, including Ihh, Fst, and Errfi1. Our findings highlight the key role of uterine Ctcf in progesterone-dependent expression and optimal post-pubertal uterine development.
Article
Cell Biology
Nadine Hosny El Said, Francesco Della Valle, Peng Liu, Andreu Paytuvi-Gallart, Sabir Adroub, Juliette Gimenez, Valerio Orlando
Summary: This study reveals the significant role of PRC2 and long non-coding RNAs in oxidative stress response, and identifies Malat-1 as the first lncRNA involved in PRC2-Ezh1 function.
CELL DEATH & DISEASE
(2021)
Article
Neurosciences
Xinyan Li, Hongyan Yu, Bing Zhang, Lanfang Li, Wenting Chen, Quntao Yu, Xian Huang, Xiao Ke, Yunyun Wang, Wei Jing, Huiyun Du, Hao Li, Tongmei Zhang, Liang Liu, Ling-Qiang Zhu, Youming Lu
Summary: Cholinergic neurons in different species can be categorized into two subpopulations, each with unique functional and structural characteristics, that contribute to diverse behavioral functions.
Article
Biochemistry & Molecular Biology
Michael Holzer, Senka Ljubojevic-Holzer, Douglas Ricardo Souza Junior, Julia T. Stadler, Alankrita Rani, Hubert Scharnagl, Graziella Eliza Ronsein, Gunther Marsche
Summary: This study compares the effects of three common methods for HDL isolation on the composition and function of HDL. The results show that different isolation methods result in HDL samples with different protein compositions and subclass distributions, as well as functional differences.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Biology
Max A. Horlbeck, Lea B. Witkowsky, Benjamin Guglielmi, Joseph M. Replogle, Luke A. Gilbert, Jacqueline E. Villalta, Sharon E. Torigoe, Robert Tjian, Jonathan S. Weissman
Article
Cell Biology
Liangqi Xie, Sharon E. Torigoe, Jifang Xiao, Daniel H. Mai, Li Li, Fred P. Davis, Peng Dong, Herve Marie-Nelly, Jonathan Grimm, Luke Lavis, Xavier Darzacq, Claudia Cattoglio, Zhe Liu, Robert Tjian
GENES & DEVELOPMENT
(2017)
Article
Microbiology
Joshua J. Smith, Sharon E. Torigoe, Julia Maxson, Lisa C. Fish, Emily A. Wiley
Article
Biochemistry & Molecular Biology
Sharon E. Torigoe, Debra L. Urwin, Haruhiko Ishii, Douglas E. Smith, James T. Kadonaga
Article
Cell Biology
Jia Fei, Sharon E. Torigoe, Christopher R. Brown, Mai T. Khuong, George A. Kassavetis, Hinrich Boeger, James T. Kadonaga
GENES & DEVELOPMENT
(2015)
