Review
Immunology
Jing Zhang, Ling Chen, Yang Yang, Xiao Liu, Yuan Yuan, Shi-rong Song, Yue Zhao, Jian Mao
Summary: The purpose of this study was to compare the clinical and laboratory characteristics of late-onset sepsis (LOS) caused by Gram-negative and Gram-positive bacteria in preterm neonates and evaluate the diagnostic accuracy of biomarkers. The results showed that LOS caused by Gram-negative bacteria in preterm neonates had higher levels of CRP and lower platelet counts. Therefore, platelet count and CRP values may be useful biomarkers to differentiate sepsis caused by Gram-negative and Gram-positive bacteria in preterm neonates.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Infectious Diseases
Khouloud Slimene, Allaaeddin El Salabi, Olfa Dziri, Najla Mathlouthi, Seydina M. Diene, Elhussan Ahmed Mohamed, Jadalla M. A. Amhalhal, Mohammed O. Aboalgasem, Jomaa F. Alrjael, Jean-Marc Rolain, Chedly Chouchani
Summary: Antimicrobial resistance, especially to carbapenems, poses a major threat to public health. A study conducted in Ibn Sina Hospital in Libya identified low susceptibility to carbapenems among Gram-negative bacteria. Molecular investigations revealed the presence of New Delhi metallo-beta-lactamases-1 and Verona integron-encoded metallo-beta-lactamase enzymes in Pseudomonas spp. Additionally, a new gene and resistance gene were discovered in Acinetobacter baumannii.
MICROBIAL DRUG RESISTANCE
(2023)
Article
Microbiology
Andre Valencio, Miriam Aparecida da Silva, Fernanda Fernandes Santos, Juliana Moutinho Polatto, Marcelo Marcondes Ferreira Machado, Roxane Maria Fontes Piazza, Ana Cristina Gales
Summary: Detection of KPC-type carbapenemases is important for guiding antibiotic therapy and infection control measures. This study aimed to develop an ELISA test to specifically detect KPC-2 and its D179 mutants. The ELISA-KPC test showed high sensitivity and specificity, successfully detecting all isolates producing carbapenemases, including those with ESBL phenotype.
Article
Multidisciplinary Sciences
Kumar Sachin Singh, Rishabh Sharma, Poli Adi Narayana Reddy, Prashanthi Vonteddu, Madeline Good, Anjana Sundarrajan, Hyeree Choi, Kar Muthumani, Andrew Kossenkov, Aaron R. Goldman, Hsin-Yao Tang, Maxim Totrov, Joel Cassel, Maureen E. Murphy, Rajasekharan Somasundaram, Meenhard Herlyn, Joseph M. Salvino, Farokh Dotiwala
Summary: Isoprenoids are essential for maintaining cellular functions and IspH is a crucial enzyme in their synthesis pathway. Through structure-guided design, a class of IspH inhibitors with nanomolar potency were identified, showing efficacy against multidrug-resistant bacteria while being relatively non-toxic to mammalian cells. These prodrugs also induce expansion and activation of human V gamma 9V delta 2 T cells, suggesting a potential strategy to limit antibiotic-resistant bacterial populations.
Article
Microbiology
Dee Shortridge, Jennifer M. Streit, Rodrigo Mendes, Mariana Castanheira
Summary: Cefiderocol is a broad-spectrum cephalosporin with activity against Gram-negative bacteria, making it a valuable therapeutic option for drug-resistant infections.
MICROBIOLOGY SPECTRUM
(2022)
Article
Agriculture, Dairy & Animal Science
Francesca Paola Nocera, Monica Ambrosio, Filomena Fiorito, Laura Cortese, Luisa De Martino
Summary: The study found that Staphylococcus and Gram-negative bacteria are the most common pathogens in skin infections of small animals, and the increasing prevalence of multi-drug-resistant strains is a concerning trend. This spread is not only significant in veterinary medicine but also has implications for zoonotic diseases.
Article
Infectious Diseases
Antonella Migliaccio, Maria Stabile, Maria Bagattini, Maria Triassi, Rita Berisio, Eliana De Gregorio, Raffaele Zarrilli
Summary: Resveratrol can reverse tolerance and restore susceptibility to CHX and BZK in the majority of microorganisms causing HAI. The efflux pump inhibitor reduced the MIC and MBC of CHX and BZK in most strains, while resveratrol dose-dependently decreased the MIC and MBC of the biocides.
Article
Public, Environmental & Occupational Health
P. Aranega-Bou, C. Cornbill, N. Q. Verlander, G. Moore
Summary: This study evaluated infection prevention strategies in reducing the risk associated with contaminated hospital sinks by comparing the dispersal of Gram-negative bacteria from different types of clinical handwash basins. The findings showed that a 'splash-reducing' CHWB had significantly lower odds of spreading contamination compared to a conventional CHWB in various contamination scenarios.
JOURNAL OF HOSPITAL INFECTION
(2021)
Article
Dentistry, Oral Surgery & Medicine
Lais Christina Pontes Espindola, Renata Cristina Picao, Stella Maria Casas Novas Mancano, Renata Martins do Souto, Ana Paula Vieira Colombo
Summary: A high prevalence and diversity of Gram-negative bacilli, with low susceptibility to beta-lactams, were observed in the subgingival microbiota associated with periodontitis.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Immunology
Shi Wu, Dandan Yin, Peiyuan Zhi, Yan Guo, Yang Yang, Demei Zhu, Fupin Hu
Summary: This study evaluated the in vitro antibacterial activity of MRX-8 against gram-negative bacteria recently isolated from China. The results showed that MRX-8 was effective against most clinically isolated gram-negative isolates, especially carbapenem-resistant bacteria.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Estefania Cano-Martin, Ines Portillo-Calderon, Patricia Perez-Palacios, Jose Maria Navarro-Mari, Maria Amelia Fernandez-Sierra, Jose Gutierrez-Fernandez
Summary: Antibiotic resistance in bacteria has been challenging to control, particularly in the case of multidrug-resistant bacteria such as carbapenemase-producing bacteria. A study conducted in a hospital in Southern Spain between 2015 and 2020 revealed an increasing trend in infections with carbapenemase-producing bacteria. The combination of epidemiological data with real-time molecular sequencing techniques can significantly advance the transmission control of these bacteria and the management of infected patients.
Article
Chemistry, Multidisciplinary
Mengjun Liu, Minmin Zhang, Jiamei Chen, Ruizhi Yang, Zhaofan Huang, Zhenping Liu, Na Li, Lingling Shui
Summary: In this study, a liquid crystal-based aptasensor was proposed and validated for the ultrasensitive detection of Escherichia coli (E. coli). By inducing changes in the optical texture through the arrangement of liquid crystal molecules, the aptasensor achieved an optical transition from bright to dark as a detection mechanism. The developed aptasensor showed high sensitivity and specificity, with an ultralow detection limit of 27 cfu/mL. This method holds great promise for the on-site detection of Gram-negative bacteria in various applications such as environmental monitoring, food safety assessment, and household chemical inspection.
SCIENCE CHINA-CHEMISTRY
(2022)
Review
Microbiology
Claire Maher, Karl A. Hassan
Summary: Gram-negative bacteria are resistant to antibiotics due to their cell envelope's permeability barrier. Understanding the role of different components in the envelope is crucial for developing new broad-spectrum antibiotics.
Article
Microbiology
Ammara Mushtaq, Rachel Chasan, Michael D. Nowak, Meenakshi Rana, Sahrish Ilyas, Alberto E. Paniz-Mondolfi, Emilia M. Sordillo, Gopi Patel, Melissa R. Gitman
Summary: This study reported the frequency of resistance gene detection in Gram-negative blood culture isolates and correlated these findings with corresponding antibiograms. The prevalence of CTX-M-producing Enterobacterales remains high in the United States, with a small number of CTX-M-positive isolates being susceptible to ceftriaxone, and a small number of ceftriaxone nonsusceptible isolates being negative for bla(CTX-M) on the ePlex BCID-GN panel.
MICROBIOLOGY SPECTRUM
(2022)
Article
Chemistry, Analytical
Sixia Ye, Ting Han, Musen Cheng, Lijie Dong
Summary: This study develops a fluorescent Gram-negative bacteria probe based on quantum dots, which can detect E. coli and P. Aeruginosa rapidly and with high sensitivity. The probe retains high selectivity and robustness in the presence of other Gram-positive bacteria.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)