Review
Pharmacology & Pharmacy
Jeonghwan Kim, Yulia Eygeris, Mohit Gupta, Gaurav Sahay
Summary: mRNA vaccines, emerging as front-runners in the COVID-19 vaccine race, utilize advancements in RNA technology, vaccinology, and nanotechnology to design self-assembled nanoparticles for vaccine delivery, showing tremendous promise and readiness for clinical use.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Chemistry, Physical
Badri P. Mainali, Dhruba K. Pattadar, Francis P. Zamborini
Summary: The oxidation resistance of gold nanoparticles has a unique reverse size dependence for thiolate-coated nanoparticles, where the extent of oxidation increases with size, opposite to weakly stabilized or bare gold nanoparticles.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Chemistry, Physical
Aila Jimenez-Ruiz, Rafael Prado-Gotor, Jose G. Fernandez-Bolanos, Alejandro Gonzalez-Benjumea, Jose Maria Carnerero
Summary: Stable oleuropein-coated gold nanoparticles were synthesized in aqueous media for the first time. The concentration of oleuropein in the reaction medium was found to significantly impact the outcome and stability of the resulting nanocolloid, with smaller particle sizes observed in more concentrated solutions. Self-assembled structures of oleuropein were identified at a specific concentration threshold, contributing to both increased stability and reduced particle size in concentrated solutions.
Article
Biochemistry & Molecular Biology
Damian Dziubak, Kamil Strzelak, Slawomir Sek
Summary: This paper investigates the formation and electrochemical properties of lipid films deposited from bicellar mixtures onto thioglucose-modified gold electrodes. It was found that the adsorption of bicelles leads to the formation of a double bilayer structure on the electrode surface, containing defects and pinholes that affect membrane permeability. Freeze-thaw treatment significantly improves morphology and electrochemical characteristics, reducing the number of pinholes and increasing membrane resistance.
Article
Chemistry, Multidisciplinary
Tingyao Zhou, Xingyu Jiang
Summary: This study presents a facile strategy for preparing Au nanoassemblies with controllable structures and tunable luminescence using Pluronic F127 as an adaptive template. The hierarchical information encryption was achieved by modulating excitation light, providing a novel pathway for creating uniform nanomaterials with desired functions for potential applications in information security.
Article
Multidisciplinary Sciences
Eugeniu Balaur, Catherine Sadatnajafi, Brian Abbey
Summary: Periodic subwavelength apertures can detect variations in the dielectric properties of the local sample environment by modifying plasmon resonances. Bimodal silver-based plasmonic colour filters show potential in molecular sensing and real-time monitoring of self-assembled monolayers. This technique enables dynamic monitoring of local environment changes without specific functionalization or labeling, and can be miniaturized for small sample analysis.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Multidisciplinary
Enrico Lavagna, Sebastian Salassi, Davide Bochicchio, Giulia Rossi
Summary: This study computationally investigates the colloidal aggregation of gold nanoparticles isotropically functionalized by a mixture of charged and hydrophobic ligands, and reveals that anisotropic aggregates with different topologies can be formed by varying the proportion of the two ligands.
Article
Polymer Science
Yanyan Zhu, Changhang Huang, Liangshun Zhang, David Andelman, Xingkun Man
Summary: The kinetic paths of structural evolution and formation of block copolymer (BCP) particles were investigated using dynamic self-consistent field theory (DSCFT). The study revealed that the self-assembly process of BCP in a poor solvent leads to the formation of striped ellipsoids, onion-like particles, and double-spiral lamellar particles. The shape transition between onion-like particles and striped ellipsoidal ones can be reversible by controlling the temperature and solvent selectivity. Additionally, changing the intermediate bi-continuous structure into a layered one is crucial for the formation of striped ellipsoidal particles.
MACROMOLECULAR RAPID COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Amar Thaker, Luqmanal Sirajudeen, Chad R. Simmons, Brent L. Nannenga
Summary: The study demonstrates that maltose-binding protein (MBP) can facilitate stable gold nanoparticle synthesis, with the structure of MBP in the presence of gold ions determined by X-ray crystallography. A peptide (AT1) selected and synthesized based on this structure also aids in the synthesis of stable gold nanoparticles, representing a new potential method for peptide selection in nanoparticle synthesis.
BIOTECHNOLOGY AND BIOENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Jack S. Googasian, George R. Lewis, Zachary J. Woessner, Emilie Ringe, Sara E. Skrabalak
Summary: Chiral plasmonic nanocrystals with varied symmetries were synthesized through overgrowth, highlighting the importance of chiral molecule adsorption at kink sites.
CHEMICAL COMMUNICATIONS
(2022)
Article
Polymer Science
Munju Shin, Sejun Yang, Hyo Won Kwak, Ki Hoon Lee
Summary: Various natural materials, including silk sericin (SS), have been used for the synthesis of gold nanoparticles (AuNPs). In this study, AuNPs were successfully synthesized using SS, and optimal synthesis conditions were determined. The size of the AuNPs prepared under the optimal conditions was approximately 17 nm, and the particles were well dispersed.
EUROPEAN POLYMER JOURNAL
(2022)
Review
Chemistry, Multidisciplinary
Aaron S. Schwartz-Duval, Konstantin Sokolov
Summary: Gold nanoparticles have great potential in biomedical applications, but there are currently no clinically approved injectable GNP formulations. Gold salts, on the other hand, have been used in the clinic for a long time and there is evidence of GNP formation through their use. Recent studies have shown that using gold ions for in situ formation of therapeutic GNPs can improve delivery within dense biological tissues, increase intracellular gold uptake efficiency, and specifically form GNPs within cancer cells. Combined with the safety of gold salts, this process can be a viable strategy for clinical translation.
Article
Electrochemistry
Xiaoyun Qin, Jin Zhang, Wenlong Shao, Xiaoli Liu, Xuemin Zhang, Fenghua Chen, Xiaomei Qin, Lan Wang, Dan Luo, Xuezhi Qiao
Summary: In this study, electrodes modified with self-assembled gold nanoparticles were used as electrochemical sensors for the detection of dopamine, achieving better signal stability and high signal reproducibility.
ELECTROCHEMISTRY COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Indra Memdi Khoris, Akhilesh Babu Ganganboina, Tetsuro Suzuki, Enoch Y. Park
Summary: By co-precipitating the chromogen TMB in aqueous solution, collective nanoparticles TMB-NPs were formed and enriched in PLGA nanovesicles using a molecular delivery approach. The developed colorimetric sensor demonstrated high sensitivity in detecting influenza virus and other respiratory viruses, showcasing practical applications for airborne virus detection.
Article
Chemistry, Multidisciplinary
Jiao-Jiao Li, Chun-Yu Liu, Zong-Jie Guan, Zhen Lei, Quan-Ming Wang
Summary: Anions have been used to regulate the structures and luminescence of heterometallic clusters. Different anions can change the emission color and structure of metal clusters. The involvement of anions leads to a shift of emission peak, and the high quantum yields of the clusters are attributed to the protection provided by ligands and anions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Review
Biochemical Research Methods
Xhensila Shkembi, Marketa Svobodova, Vasso Skouridou, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Ciara K. O'Sullivan
Summary: This review discusses the selection of aptamers against mycotoxins and their incorporation into optical and electrochemical aptasensors. The strategies, advantages, and disadvantages of each approach are outlined, along with the different materials used and immobilization methods employed to achieve the highest sensitivity and selectivity.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
David Kodr, Cansu Pinar Yenice, Anna Simonova, Dijana Pavlovic Saftic, Radek Pohl, Veronika Sykorova, Mayreli Ortiz, Ludek Havran, Miroslav Fojta, Zbigniew J. Lesnikowski, Ciara K. O'Sullivan, Michal Hocek
Summary: A series of modified nucleoside triphosphates bearing dicarba-nido-undecaborate or metal bis(dicarbollide) groups were prepared and utilized for DNA synthesis and redox labeling. The combination of these redox labels allowed for the first fully orthogonal redox coding of all four canonical nucleobases, enabling determination of nucleobase composition of short DNA stretches with electrochemical readout in one simple primer extension experiment.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Analytical
Miriam Jauset-Rubio, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: Isothermal recombinase polymerase amplification-based solid-phase primer extension is utilized for the optical detection of a single nucleotide polymorphism related to hypertrophic cardiomyopathy in fingerprick blood samples. By optimizing the percentage of biotinylated dNTPs and the duration of primer extension, the SNP in the fingerprick sample was identified successfully. Ongoing work is focused on expanding the technology to the multiplexed detection of SNPs in fingerprick and other biological samples.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Xhensila Shkembi, Vasso Skouridou, Marketa Svobodova, Sandra Leonardo, Abdulaziz S. Bashammakh, Abdulrahman O. Alyoubi, Monica Campas, Ciara K. O'Sullivan
Summary: This study developed an aptamer-based detection method for the rapid and sensitive detection of TTX in pufferfish, using next-generation sequencing technology. The results demonstrated that the developed hybrid antibody-aptamer sandwich assay outperformed several methods reported in the literature and commercial immunoassay kits.
ANALYTICAL CHEMISTRY
(2021)
Article
Biophysics
Mayreli Ortiz, Miriam Jauset-Rubio, David Kodr, Anna Simonova, Michal Hocek, Ciara K. O'Sullivan
Summary: This study developed an electrochemical platform for the detection of SNP in the MYH7 gene associated with hypertrophic cardiomyopathies using fingerprick blood samples. The platform uses solid-phase primer elongation and recombinase polymerase amplification to accurately identify SNPs, and only requires a small amount of fingerprick blood for analysis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Materials Science, Biomaterials
Muge Asik, Ismail Kutlugun Akbay, Sadin Ozdemir, Rukan Genc
Summary: pH-responsive self-healing hydrogels from cross-linked tragacanth gum (TG) were developed as a controlled antibiotic release system. These hydrogels can sustain multiple cycles of healing and separation while maintaining their mechanical and healing properties. The loaded Cf-TG hydrogels showed slow drug release and excellent antibacterial activity against both gram-negative and gram-positive bacteria, with a robust inhibition of biofilm formation.
INTERNATIONAL JOURNAL OF POLYMERIC MATERIALS AND POLYMERIC BIOMATERIALS
(2023)
Article
Polymer Science
Didem Demir, Muge Asik Ugurlu, Seda Ceylan, Burcu Sakim, Rukan Genc, Nimet Bolgen
Summary: This study investigated the characteristics of chitosan-gum tragacanth polysaccharide scaffolds. Porous scaffolds with suitable mechanical properties and biocompatibility were successfully fabricated through cryotropic gelation.
POLYMER INTERNATIONAL
(2022)
Article
Environmental Sciences
Greta Gaiani, Francesca Cucchi, Anna Toldra, Karl B. Andree, Maria Rey, Takeshi Tsumuraya, Ciara K. O'Sullivan, Jorge Diogene, Monica Campas
Summary: Several genera of marine dinoflagellates are capable of producing bioactive compounds that can impact human health, with Gambierdiscus and Fukuyoa standing out for their production of toxins, including ciguatoxins. A multiplex detection system and rapid DNA extraction technique have been developed for identifying toxin-producing species, enabling swift detection in field samples.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Chemistry, Multidisciplinary
Mayreli Ortiz, Miriam Jauset-Rubio, Vasso Skouridou, Diana Machado, Miguel Viveiros, Taane G. Clark, Anna Simonova, David Kodr, Michal Hocek, Ciara K. O'Sullivan
Summary: In this study, electrochemical detection of single point mutations using solid-phase isothermal primer elongation with redox-labeled oligonucleotides was reported. By assembling four S'-thiolated primers on individual gold electrodes, successful electrochemical detection of single-point mutations in genomic DNA extracted from Mycobacterium tuberculosis strains was achieved, demonstrating a rapid, cost-effective, and facile method for genotyping.
Article
Chemistry, Analytical
Koena L. Moabelo, Teresa M. Lerga, Miriam Jauset-Rubio, Nicole R. S. Sibuyi, Ciara K. O'Sullivan, Mervin Meyer, Abram M. Madiehe
Summary: This study presents the development of a rapid aptasensor based on gold nanoparticles for the detection of RBP4. The method utilizes the specific binding of aptamers to RBP4 and the color change of gold nanoparticles to achieve rapid detection, with results available within 5 minutes.
Article
Chemistry, Analytical
Miriam Jauset-Rubio, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: Aptamer-based assays and sensors show promise as alternatives to antibodies for their flexibility in assay formats. This study demonstrates the use of a biotin-modified aptamer in enhancing sensitivity and achieving lower detection limits in a colorimetric enzyme-linked oligonucleotide assay and electrochemical detection. The generic nature of this assay format allows for easy and cost-effective application to various aptamers.
Article
Chemistry, Analytical
David Kodr, Mayreli Ortiz, Veronika Sykorova, Cansu Pinar Yenice, Zbigniew J. Lesnikowski, Ciara K. O'Sullivan, Michal Hocek
Summary: The previous approach of orthogonal multipotential redox coding only allowed analysis of relative nucleotide composition of short DNA stretches. In this study, two methods for normalization of electrochemical readout were presented, enabling determination of total nucleotide composition. The first method involved the presence or absence of an internal standard in the DNA primer, while the second method utilized a viologen-modified primer acting as a reference label for signal normalization. Both methods accurately analyzed DNA sequences and demonstrated potential for targeted DNA sequence analysis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Cansu Pinar Yenice, Nassif Chahin, Miriam Jauset-Rubio, Matthew Hall, Phil Biggs, Hans-Peter Dimai, Barbara Obermayer-Pietsch, Mayreli Ortiz, Ciara K. O'Sullivan
Summary: The detection of single nucleotide polymorphisms (SNPs) is increasingly important in various fields. This study presents a semi-automated system for solid-phase electrochemical melting curve analysis (e'MCA) to identify the allele present at a specific SNP site associated with an increased risk of bone fracture and predisposition to osteoporosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Mayreli Ortiz, Miriam Jauset-Rubio, Olivia Trummer, Ines Foessl, David Kodr, Josep Lluis Acero, Mary Luz Botero, Phil Biggs, Daniel Lenartowicz, Katerina Trajanoska, Fernando Rivadeneira, Michal Hocek, Barbara Obermayer-Pietsch, Ciara K. O'Sullivan
Summary: A generic platform was developed for cost-effective and rapid electrochemical SNP genotyping from fingerprick blood samples, enabling early diagnosis of osteoporosis. The platform utilizes isothermal solid-phase primer elongation with ferrocene-labeled nucleoside triphosphates, allowing for targeted genotyping of SNPs associated with osteoporosis. The system was validated using real blood samples, with results matching those from Taqman SNP genotyping assays and Sanger sequencing. The platform is highly versatile and has the potential to be deployed in an automated device for targeted SNP genotyping.
ACS CENTRAL SCIENCE
(2023)
