Article
Biotechnology & Applied Microbiology
Hongrao Wang, Shuran Han, Jiayi Wang, Shuyu Yu, Xiaoyan Li, Lei Lu
Summary: The immobilized laccase as magnetic cross-linked enzyme aggregates (M-CLEAs) showed promising activity recovery and stability for dye decolorization, especially under alkaline conditions. Additionally, M-CLEAs exhibited enhanced tolerance towards NaCl and were able to rapidly decolorize various dyes.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Materials Science, Multidisciplinary
Murat Uygun, Victor de la Asuncion-Nadal, Sinem Evli, Deniz Akta Uygun, Beatriz Jurado-Sanchez, Alberto Escarpa
Summary: Laccase-based micromotors were synthesized and used for decolorization of azo dyes in textile wastewater, showing improved dye removal capacity compared to the free enzyme approach. The stability of the immobilized enzyme was enhanced, resulting in higher decolorization efficiencies of 76-94% compared to 34-55% using free enzyme.
APPLIED MATERIALS TODAY
(2021)
Article
Environmental Sciences
Jenet George, Devi Rajendran, P. Senthil Kumar, Srinidhi Sonai Anand, Vaidyanathan Vinoth Kumar, Gayathri Rangasamy
Summary: In this study, porous-cross-linked enzyme aggregates (CLEAs) of Pleurotus ostreatus laccase were used for the decolorization and detoxification of triarylmethane and azo dyes. The CLEAs laccase showed high efficiency in decolorizing malachite green (MG) and reactive blue 2 (RB) without a mediator, with decolorization rates of 58.33-98.12% after 120 minutes. The immobilized laccase was able to effectively remove dyes from aqueous solutions and demonstrated significant detoxification in experimental plants and plant growth-promoting rhizobacteria. This suggests that porous CLEAs laccase could be a potential tool for the bioremediation of dyeing wastewater.
Article
Green & Sustainable Science & Technology
Itzel C. Romero-Soto, Raul B. Martinez-Perez, Jorge A. Rodriguez, Rosa M. Camacho-Ruiz, Alejandra Barbachano-Torres, Martha Martin del Campo, Juan Napoles-Armenta, Jorge E. Pliego-Sandoval, Maria O. Concha-Guzman, Maria Angeles Camacho-Ruiz
Summary: The study demonstrates that laccase produced from the ascomycete Gliomastix murorum can efficiently decolorize Reactive Blue 2 without the addition of redox mediators when entrapped in galactomannan. The entrapped laccase shows good reusability, stability, and efficiency, making it a promising candidate for large-scale wastewater treatment. The use of galactomannan for laccase entrapment offers economic advantages due to its natural origin and effective results.
Article
Polymer Science
Fevziye Unlu, Filiz Boran, Ozfer Yesilada, Suleyman Koytepe
Summary: The study aimed to immobilize laccase on dry bacterial cellulose (BC) produced by Komagataeibacter xylinus B759 in mulberry pomace waste extract as a low-cost and natural waste by-product, and to evaluate the effectiveness of the obtained laccase immobilized BC (Lac/BC) for textile dye decolorization. The BC and Lac/BC structures were characterized, and the morphological properties and fiber structure of the BC and enzyme-linked cellulose structures were examined. The results showed that BC produced in a mulberry pomace waste extract can be used for laccase immobilization and Lac/BC is an effective and promising system for textile dye decolorization.
JOURNAL OF APPLIED POLYMER SCIENCE
(2023)
Article
Chemistry, Physical
Sadia Aslam, Awais Ali, Muhammad Asgher, Nabila Farah, Hafiz M. N. Iqbal, Muhammad Bilal
Summary: The immobilization of laccase on Ca-alginate beads effectively decolorized various dye pollutants and reduced COD and TOC levels. This cost-effective and simple method shows promise for environmental remediation applications.
Article
Multidisciplinary Sciences
Hyeryeong Lee, Eun Mi Lee, Stacy Simai Reginald, In Seop Chang
Summary: This study compared the effects of different gold-binding peptides fused to enzymes on electrocatalytic activity, bioactivity, and material-binding behaviors, demonstrating the vital role of GBP type in the design, construction, and optimization of enzyme-modified electrodes for efficient interfacial DET in DET-based enzyme-electrode systems.
Article
Biochemistry & Molecular Biology
Navleen Kaur Chopra, Sonica Sondhi
Summary: The laccase gene from Bacillus licheniformis NS2324 was cloned and expressed in E. coli, and its properties and activities were studied. The results showed that the recombinant laccase exhibited good tolerance and decolorization ability, making it suitable for eco-friendly and cost-effective dye decolorization in industrial applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Environmental Sciences
Emre Birhanli, Samir Abbas Ali Noma, Filiz Boran, Ahmet Ulu, Ozfer Yesilada, Burhan Ates
Summary: This study aims to present a simple and effective carrier matrix for immobilizing laccase and remove textile dyes. Two different metal-organic frameworks (MOFs) based on Cobalt (Co) and Copper (Cu) were prepared and laccase was successfully immobilized via encapsulation. The immobilized laccases showed good reusability, resistance to temperature denaturation, high storage stability, and improved thermostability. The MOFs also exhibited good decolorization activities for textile dyes.
Article
Biochemistry & Molecular Biology
Xue Yang, Jing Zhao, Artur Cavaco-Paulo, Jing Su, Hongbo Wang
Summary: Enzyme immobilization provides enhanced stability for laccase and improves the adsorption and degradation efficiency of pollutants. In this study, bimetallic Cu/Zn ZIFs were used as a protective carrier for laccase, showing high operational stability and efficient decolorization of dye wastewater. The encapsulated laccase could be recovered and reused multiple times, indicating great potential for water remediation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Microbiology
Marina Gutierrez-Anton, Alejandro Santiago-Hernandez, Johan Rodriguez-Mendoza, Claudia Cano-Ramirez, Ismael Bustos-Jaimes, Guillermo Aguilar-Osorio, Jorge E. Campos, Maria Eugenia Hidalgo-Lara
Summary: A 32-fold increase in laccase activity production was achieved by the thermophilic fungus T. terrestris Co3Bag1 when grown on a modified medium containing fructose, sodium nitrate, and copper. A 70 kDa laccase (TtLacA) was purified, immobilized in copper alginate gel beads, and characterized. The immobilized TtLacA showed higher thermal stability and retained high activity after multiple reuse cycles and storage at low temperature.
Review
Chemistry, Applied
Zahra Shokri, Farzad Seidi, Shiva Karami, Chengcheng Li, Mohammad Reza Saeb, Huining Xiao
Summary: This review paper discusses the significance and potential of using natural polysaccharides as support materials for enzyme immobilization, especially laccases, to improve their stability and selectivity in industrial applications. Additionally, the roles of immobilized laccases are examined from a fundamental perspective to elucidate their catalytic mechanisms in detecting and degrading environmental contaminants.
CARBOHYDRATE POLYMERS
(2021)
Article
Biotechnology & Applied Microbiology
Nikolina Popovic, Marija Stanisic, Karla Ilic Durdic, Olivera Prodanovic, Natalija Polovic, Radivoje Prodanovic
Summary: In this study, a modified pectin was synthesized and used to produce dopamine-pectin microbeads with immobilized laccase through enzymatic crosslinking. The immobilized laccase showed improved thermal and pH stability, effectively decolorizing various dyes. The microbead immobilized laccase maintained high decolorization efficiency even after ten cycles of repeated use.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Chemistry, Physical
Yingying Huang, Lihui Tan, Meng Chen, Yanpeng Jiao, Jinhuan Tian, Lihua Li, Changren Zhou, Lu Lu
Summary: The removal of dye molecules from water sources is a major concern. Enzymatic degradation and adsorptive dye removal are effective techniques. In this study, stable Pickering emulsions were prepared using chitin and graphene oxide solutions. The immobilized laccase showed better thermal stability compared to the free enzyme, and the preparation method had potential industrial applications in dye wastewater treatment.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2023)
Article
Engineering, Environmental
Yan Zhang, Thea Jess Plesner, Yi Ouyang, Yu-Cong Zheng, Etienne Bouhier, Emilie Ingemann Berentzen, Mingliang Zhang, Pengfei Zhou, Wolfgang Zimmermann, Gregers Rom Andersen, Bekir Engin Eser, Zheng Guo
Summary: A novel thermophilic laccase, LfLAC3, was identified from PE-degrading Lysinibaccillus fusiformis through computer-aided and activity-based screening. LfLAC3 showed high robustness and catalytic promiscuity. It was able to degrade all tested dyes and low-density polyethylene films without the use of a mediator, indicating its potential for dye decolorization and plastic degradation.
JOURNAL OF HAZARDOUS MATERIALS
(2023)
Article
Engineering, Chemical
Burcu Okutucu, Senay Hamarat Sanlier
DESALINATION AND WATER TREATMENT
(2016)
Article
Polymer Science
Yeliz Yildirim, Habibe Yilmaz, Guliz Ak, Senay Hamarat Sanlier
POLIMEROS-CIENCIA E TECNOLOGIA
(2015)
Article
Biotechnology & Applied Microbiology
Senay Hamarat Sanlier, Merve Yasa, Asli Ozge Cihnioglu, Merve Abdulhayoglu, Habibe Yilmaz, Guliz Ak
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2016)
Article
Oncology
Selvi Gunel Nur, Ozel Buket, Kipcak Sezgi, Aktan Cagdas, Akgun Cansu, Ak Guliz, Yilmaz Habibe, Biray Avci Cigir, Dodurga Yavuz, Hamarat Sanlier Senay
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2016)
Article
Biotechnology & Applied Microbiology
Guliz Ak, Habibe Yilmaz, Aybike Gunes, Senay Hamarat Sanlier
ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY
(2018)
Article
Chemistry, Medicinal
Senay Hamarat Sanlier, Guliz Ak, Habibe Yilmaz, Ayse Unal, Ummuhan Fulden Bozkaya, Gokce Taniyan, Yeliz Yildirim, Gulbeyaz Yildiz Turkyilmaz
JOURNAL OF PHARMACEUTICAL SCIENCES
(2019)
Article
Biochemical Research Methods
Guliz Ak, Didem Aksu, Eda Capkin, Ozge Sari, Ilgin Kimiz Gebologlu, Senay Hamarat Sanlier
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
(2020)
Article
Chemistry, Physical
A. K. Guliz, Senay Hamarat Sanlier
JOURNAL OF MOLECULAR STRUCTURE
(2020)
Article
Pharmacology & Pharmacy
Guliz Ak, Ummuhan Fulden Bozkaya, Habibe Yilmaz, Ozge Sari Turgut, Ismail Bilgin, Canberk Tomruk, Yigit Uyanikgil, Senay Hamarat Sanlier
Summary: A novel carrier system, gentamicin-loaded magnetic gelatin nanoparticles, was developed for osteomyelitis therapy, showing improved therapeutic potential through magnetically targeted and nanocarrier-mediated delivery. Optimized GMGNPs exhibited controlled drug release profile and significant efficacy in a rat model of osteomyelitis.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Oncology
Guliz Ak, Tugba Karakayali, Ayse N. Cin, Buket Ozel, Senay H. Sanlier
Summary: The study successfully synthesized Doxorubicin and Curcumin loaded magnetic nanoparticles using green tea leaves and demonstrated a synergistic antitumor activity against the A549-luc-C8 cell line.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2021)
Article
Biophysics
Guliz Ak, Ayse Unal, Tugba Karakayali, Buket Ozel, Nur Selvi Gunel, Senay Hamarat Sanlier
Summary: The study demonstrates that BCNU and TMZ loaded SLNs can serve as an effective anticancer system for targeted GBM therapy, showing significant antitumor activity and inducing apoptosis on cancer cells, as well as being more readily taken up by MCT-1 expressing brain cells.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Chemistry, Medicinal
Guliz Ak, Irfan Akartas, Buket Ozel, Nur Selvi Gunel, Hatice Yesim Karasulu, Baris Gumustas, Ercument Karasulu, Senay Hamarat Sanlier
Summary: The study successfully prepared a cisplatin-bound triblock polymeric micelle solution for the treatment of ovarian cancer, demonstrating biocompatibility and permeability in vitro studies. The cytotoxicity of the solution was found to be highest compared to cisplatin solution and conjugate in SKOV-3 and OVCAR-3 cells. Additionally, maximum apoptosis results were observed in A2780 cells.
DRUG DEVELOPMENT AND INDUSTRIAL PHARMACY
(2021)
Article
Chemistry, Multidisciplinary
Habibe Yilmaz, Guliz Ak, Gulce Ozmen, Ercument Karasulu, Senay Hamarat Sanlier
Summary: SPR methods are increasingly accepted in the biopharmaceutical industry, but there are few reports on method validation and system suitability testing related to concentration determination methods for drug potency. A study developed a system suitability testing for an SPR-based active concentration measurement method using a Fab-fragment model, confirming that the system and method are suitable for concentration analysis of the FAB1 fragment.
JOURNAL OF THE MEXICAN CHEMICAL SOCIETY
(2021)
Review
Oncology
Ibrahim Diori Karidio, Senay Hamarat Sanlier
Summary: Cancer is a global issue that researchers have yet to fully understand its complex molecular and cellular mechanisms. The mechanisms of cancer involve cell cycle, DNA integrity, genetic defects, and other factors, with different types of cancer having distinct etiology and characteristics.
JOURNAL OF THE EGYPTIAN NATIONAL CANCER INSTITUTE
(2021)
Article
Health Care Sciences & Services
Ayse Unal, Guliz Ak, Senay Hamarat Sanlier
JOURNAL OF BASIC AND CLINICAL HEALTH SCIENCES
(2020)