Article
Microbiology
S. Diaz-Diaz, E. Recacha, Marina R. Pulido, Maria Romero-Munoz, B. de Gregorio-Iaria, F. Docobo-Perez, A. Pascual, J. M. Rodriguez-Martinez
Summary: The suppression of the SOS response and Dam methylation system enhances the in vitro activity of quinolones. Dual targeting of the recA (SOS response) and Dam methylation system genes increases the sensitivity of Escherichia coli to quinolones, even in a resistant strain model. This genetic and microbiological approach demonstrates the potential for preventing the evolution of resistance by suppressing both systems in quinolone-resistant strains.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Review
Environmental Sciences
Dong Ding, Bin Wang, Xiaoan Zhang, Junxi Zhang, Huanhuan Zhang, Xinxin Liu, Zhan Gao, Zengli Yu
Summary: Antibiotic resistance poses a significant threat to human health, and reducing the spread and burden of antibiotic resistance requires implementing control strategies.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Microbiology
Adeline Supandy, Heer H. Mehta, Truc T. Tran, William R. Miller, Rutan Zhang, Libin Xu, Cesar A. Arias, Yousif Shamoo
Summary: Infections caused by vancomycin-resistant Enterococcus faecium (VREfm) are a significant public health threat. The study investigates the potential for VREfm to develop resistance to the combination therapy of daptomycin (DAP) and fosfomycin (FOS), and whether there are genetic drivers that may decrease the efficacy of the combination or delay the onset of resistance. The results show that the combination therapy can effectively inhibit VREfm without substantial cross-drug epistasis, and the addition of FOS can prolong the efficacy of DAP and slow down DAP resistance.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Environmental Sciences
Bo-Yu Su, You-Shao Wang, Cui-Ci Sun
Summary: Using transcriptome sequencing, the interspecific differences in the molecular response mechanisms of three mangrove plants (Avicennia marina, Aegiceras corniculatum, and Bruguiera gymnorrhiza) under waterlogging stress were studied. The results showed that the plants had different adaptability to waterlogging, and the differentially expressed genes (DEGs) with high folds were classified into four categories: structural regulation, transport, biosynthesis, and protection. A. Marina showed strong regulation ability in the metabolic process, while Ae. corniculatum down-regulated genes related to cell wall regulation or ethylene formation. Additionally, genes involved in alcohol dehydrogenase and lactate dehydrogenase were found to be important in the response of mangrove plants to waterlogging stress.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Food Science & Technology
Kye-Hwan Byun, Sang Ha Han, Min Woo Choi, Si Hong Park, Sang-Do Ha
Summary: The study found that sublethal concentrations of bactericidal antibiotics increased the mutation frequency in L. monocytogenes, facilitated the adaptation of the bacterium to food-associated stress, and led to an increase in its pathogenicity.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Environmental Sciences
Ruijie Zhang, Xuming Xu, Dantong Jia, Yitao Lyu, Jingrun Hu, Qian Chen, Weiling Sun
Summary: This study found that antibiotics inhibit denitrification, but sediments help alleviate this inhibition and promote bacterial growth. Sediments have negative effects on antibiotic resistance genes but positive effects on bacterial quantity and denitrification functional genes.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Multidisciplinary Sciences
Mehrose Ahmad, Hannah Prensky, Jacqueline Balestrieri, Shahd ElNaggar, Angela Gomez-Simmonds, Anne-Catrin Uhlemann, Beth Traxler, Abhyudai Singh, Allison J. Lopatkin
Summary: In this study, the authors reveal that acquiring plasmids imposes a temporary burden on bacterial hosts, resulting in a tradeoff between growth and lag time. Intermediate-cost plasmids show a competitive advantage over low and high-cost plasmids. These findings have important implications for predicting the ecological outcomes and intervention strategies of bacteria undergoing conjugation.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Qian Li, Shang Chen, Kui Zhu, Xiaoluo Huang, Yucheng Huang, Zhangqi Shen, Shuangyang Ding, Danxia Gu, Qiwen Yang, Hongli Sun, Fupin Hu, Hui Wang, Jiachang Cai, Bing Ma, Rong Zhang, Jianzhong Shen
Summary: Acquisition of resistance to one antibiotic can sometimes increase susceptibility to a second antibiotic. In this study, it was found that vancomycin-resistant Enterococcus faecium strains showed remarkable increase in susceptibility to pleuromutilin antibiotics (such as lefamulin). The trade-off between vancomycin and pleuromutilins is mediated by epistasis between van gene cluster and msrC, suggesting pleuromutilins may be useful in treating vancomycin-resistant E. faecium infections.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Shai Pilosof
Summary: Plasmids play a role in shaping the diversity, structure, and function of microbial communities. However, we lack a detailed understanding of how community structure and dynamics emerge from microbe-plasmid interactions and coevolution. This article suggests using agent-based evolutionary modeling and network analysis to quantify the importance of local processes in governing community dynamics, which can advance our understanding of plasmid ecology and evolution when combined with empirical data.
TRENDS IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Ian W. Campbell, Karthik Hullahalli, Jerrold R. Turner, Matthew K. Waldor
Summary: In this study, the authors investigated the effect of pathogen dose on the initiation of infection in the mouse gut using Citrobacter rodentium as a model. They found that host bottlenecks prevent infections by eliminating invading pathogens, and the size of the pathogen's founding population in female mice is controlled by a severe bottleneck. The disruption of the microbiota was found to be the dominant bottleneck, while the loss of the critical virulence island led to a contraction in the diversity of the pathogen population.
NATURE COMMUNICATIONS
(2023)
Article
Engineering, Environmental
Zhong-Xing Zhang, Xiao-Yan Fan, Xing Li, Yu-Xi Gao, Jun-Ru Zhao
Summary: The microbial communities in activated sludge can be influenced by antibiotics pollution. The legacy effect of historical antibiotic stress on the responses of microbes and antibiotic resistance genes (ARGs) to combined antibiotics stress is still unclear. In this study, sulfamethoxazole (SMX) or trimethoprim (TMP) was used as historical stress to explore the responses of activated sludge to combined SMX and TMP stress. The legacy effect affected the ammonia removal and the changes of microbial community structure and composition under antibiotics stress. ARGs could drive the enrichment of their potential hosts.
JOURNAL OF WATER PROCESS ENGINEERING
(2023)
Article
Microbiology
Ronika De, Marvin Whiteley, Rajeev K. Azad
Summary: This article introduces a gene co-expression network-based method for identifying pathogenicity-associated genes. By combining standard protocols with gene network methods, the ability to identify these genes in pathogens can be improved.
Article
Engineering, Environmental
Lin Wang, Zhaoli Wang, Fan Wang, Yue Guan, Dan Meng, Xin Li, Houzhen Zhou, Xudong Li, Yangwu Chen, Zhouliang Tan
Summary: This study evaluated the long-term effects of compound antibiotics on the transition from nitrification to SCND process. The results showed that compound antibiotics can promote this transition and improve the removal efficiency of total nitrogen. The study also found that SCND process has a stronger resistance to antibiotics and the spread of antibiotic resistance genes can be controlled by regulating environmental factors.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Environmental Sciences
Daniel J. Duarte, Caterina Zillien, Martine Kox, Rik Oldenkamp, Bas van der Zaan, Erwin Roex, Ad M. J. Ragas
Summary: This study conducted a one-year investigation in the city of Nijmegen, The Netherlands to identify various urban sources of antibiotics and antibiotic resistant genes (ARGs) in wastewater. The findings highlight the significant role of hospitals in the prevalence and spread of ARGs in urban wastewater. The study also suggests that changes in therapeutic regimens prescribed in hospitals may lead to shifts in ARG abundance patterns in hospital wastewater. The methods used in this study provide an opportunity to identify emission hotspots and prioritize interventions to limit the spread of ARGs from urban wastewater to the environment.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Chemistry, Multidisciplinary
Zhigang Yu, Emily C. A. Goodall, Ian R. R. Henderson, Jianhua Guo
Summary: This study reveals that plasmid-free bacterial cells exhibit filamentation and asymmetrical cell division under exposure to sub-inhibitory concentrations of antibiotics, while plasmid-bearing cells do not show such morphological changes. Higher expression of sulA is observed in plasmid-free cells. The benefits of plasmid carriage include less DNA damage, stronger efflux pump activities, and higher antibiotic tolerance.
Article
Biochemical Research Methods
Peter B. Otoupal, Keesha E. Erickson, Antoni Escalas-Bordoy, Anushree Chatterjee
ACS SYNTHETIC BIOLOGY
(2017)
Article
Chemistry, Multidisciplinary
Dodderi Manjunatha Sagar, Lee Erik Korshoj, Katrina Bethany Hanson, Partha Pratim Chowdhury, Peter Britton Otoupal, Anushree Chatterjee, Prashant Nagpal
Article
Biochemical Research Methods
Keesha E. Erickson, James D. Winkler, Danh T. Nguyen, Ryan T. Gill, Anushree Chatterjee
ACS SYNTHETIC BIOLOGY
(2017)
Article
Microbiology
Keesha E. Erickson, Peter B. Otoupal, Anushree Chatterjee
Review
Oncology
Keesha E. Erickson, Oleksii S. Rukhlenko, Richard G. Posner, William S. Hlavacek, Boris N. Kholodenko
SEMINARS IN CANCER BIOLOGY
(2019)
Article
Microbiology
Thomas R. Aunins, Keesha E. Erickson, Nripesh Prasad, Shawn E. Levy, Angela Jones, Shristi Shrestha, Rick Mastracchio, Louis Stodieck, David Klaus, Luis Zea, Anushree Chatterjee
FRONTIERS IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Logan T. Collins, Peter B. Otoupal, Jocelyn K. Campos, Colleen M. Courtney, Anushree Chatterjee
Article
Microbiology
Peter B. Otoupal, Masakazu Ito, Adam P. Arkin, Jon K. Magnuson, John M. Gladden, Jeffrey M. Skerker
Article
Biochemistry & Molecular Biology
Peter B. Otoupal, Brady F. Cress, Jennifer A. Doudna, Joseph S. Schoeniger
Summary: The study presents a novel approach, CRISPR-RNAa, for post-transcriptional control of translation to activate gene expression. The researchers develop a programmable activator of translation, dCasRx-IF3, by utilizing CRISPR-Cas13 technology. The tool demonstrates the ability to enhance gene expression and regulate endogenous genes without the need for additional host modification.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Gary R. Abel, Lee E. Korshoj, Peter B. Otoupal, Sajida Khan, Anushree Chatterjee, Prashant Nagpal
Article
Biology
Peter B. Otoupal, William T. Cordell, Vismaya Bachu, Madeleine J. Sitton, Anushree Chatterjee
COMMUNICATIONS BIOLOGY
(2018)
Article
Biotechnology & Applied Microbiology
Thomas S. Reynolds, Colleen M. Courtney, Keesha E. Erickson, Lisa M. Wolfe, Anushree Chatterjee, Prashant Nagpal, Ryan T. Gill
BIOTECHNOLOGY AND BIOENGINEERING
(2017)
Article
Microbiology
Keesha E. Erickson, Nancy E. Madinger, Anushree Chatterjee
GENOME ANNOUNCEMENTS
(2017)
Article
Biotechnology & Applied Microbiology
Peter B. Otoupal, Anushree Chatterjee
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2018)