期刊
ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
卷 2, 期 3, 页码 175-182出版社
ELSEVIER
DOI: 10.1016/j.algal.2013.01.010
关键词
Chlorophyll fluorescence transient; Dihomo-gamma-linolenic acid; Nitrogen deficiency; OJIP; On-line monitoring; Parietochloris incisa
资金
- Ministry of Science and Education of Russian Federation 'Skolkovo' Scientific Fund
- PTT Global Chemical Public Company Limited (Thailand)
- European Commission's Seventh Framework Programme for Research and Technology Development (FP7), project SENSBIOSYN [232522]
We investigated effects of irradiance on the relationships between chlorophyll fluorescence transients (OJIP), carotenoid-to-chlorophyll ratio, and fatty acids in a nitrogen-deprived Parietochloris incisa (Chlorophyta,Trebouxiophyceae) Delta 5 desaturase mutant accumulating valuable LC-PUFA dihomo-gamma-linolenic acid (DGLA). High light (270 mu E . m(-2) . s(-1) PAR) and nitrogen starvation brought about a decrease in maximum quantum yield of photosystem II (Phi(P0)) and electron transport (Phi(E0)) but enhanced the quantum yield of thermal dissipation (Phi(D0)) and induced non-photochemical quenching (NPQ) in an irradiance-dependent manner. Under high irradiance a decline in the rate of total fatty acid accumulation and DGLA percentage in comparison with the cultures grown under 130 mu E . m(-2) . s(-1) PAR was recorded. Increasing irradiance from 130 to 270 mu E . m(-2) . s(-1) enhanced total fatty acid accumulation only within the first week of nitrogen starvation and negatively affected DGLA production. Regardless of irradiance, Phi(P0), Phi(E0), and Phi(D0) exhibited tight (r(2)= 0.8-0.9) relationships with the stress-induced changes of total fatty acid and DGLA content and the carotenoid-to-chlorophyll ratio. The applicability and limitations of OJIP and its derived parameters for on-line monitoring of physiological condition and accumulation of value-added products in microalgal cultures grown in photobioreactors are discussed. (C) 2013 Elsevier B. V. All rights reserved.
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