Article
Chemistry, Multidisciplinary
Chloe Grazon, Margaret Chern, Patrick Lally, R. C. Baer, Andy Fan, Sebastien Lecommandoux, Catherine Klapperich, Allison M. Dennis, James E. Galagan, Mark W. Grinstaff
Summary: This study systematically compared the effects of organic and inorganic dyes in biosensors, as well as the impact of the position of FRET donors or acceptors on biomolecular components on sensor performance. By examining different biosensor configurations through experimental and computational approaches, researchers identified key molecular parameters influencing sensor performance and provided design guidelines for future intermolecular biosensors based on FRET.
Article
Cell Biology
Renjie Liao, Manas Mondal, Christopher D. Nazaroff, Diego Mastroeni, Paul D. Coleman, Joshua Labaer, Jia Guo
Summary: The method presented achieves highly sensitive and multiplexed protein analysis using cleavable fluorescent tyramide and off-the-shelf antibodies, enhancing detection sensitivity and reducing imaging time. By studying genetically identical cell populations and profiling neurons in human brain tissue, it reveals protein expression heterogeneity and the presence of distinct neuron clusters within different sub-regions of the hippocampus.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Food Science & Technology
Hong-Xin Ren, Min-Xin Mao, Min Li, Cun-Zheng Zhang, Chi-Fang Peng, Jiang-Guo Xu, Xin-Lin Wei
Summary: This report proposed a fluorescent sensing method for paraquat based on gold nanoclusters. The modification of beta-cyclodextrins on the surface of AuNCs enhanced the sensitivity and selectivity of the detection. With optimized conditions, the method demonstrated a linear response and high recoveries when applied to paraquat-spiked water samples. This study highlights the potential of host molecule modification of fluorescent metal NC surfaces in developing robust sensors.
Article
Chemistry, Analytical
Jizhong Wu, Waqas Ahmad, Qin Ouyang, Jingui Zhang, Mingming Zhang, Quansheng Chen
Summary: A regenerative flexible upconversion-fluorescence biosensor was designed for detecting DES in food and environmental samples through a FRET system, showing good linear relationships. It demonstrated a spiked recovery rate of 77.91-97.95% in real environments and maintained an accuracy threshold of 80% after being regenerated seven times, indicating practicality and reusability.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Multidisciplinary
Wencheng Zhong, Kangqiang Liang, Wenfeng Liu, Li Shang
Summary: In this study, a new design of Forster resonance energy transfer-based dual-color photoswitchable fluorescent nanoparticles (DPF NPs) was reported by using ligand-protected gold nanoclusters (AuNCs). The ultrasmall-sized AuNCs act as the FRET donors and also regulate the properties of spiropyran through ligand-spiropyran interactions. These DPF NPs exhibit high fluorescence on/off ratios for both green and red fluorescence emission, and show good reversibility during cycled photo-stimulation. Cell imaging experiments demonstrated that DPF NPs can specifically accumulate in lipid droplets and enable photoswitchable dual-color imaging in living cells.
Article
Chemistry, Analytical
Ruru Gao, Junhong Wang, Han Wang, Wei Dong, Junwu Zhu
Summary: A new method based on coordination polymer nanoparticles for the selective and sensitive determination of aqueous 2,4,6-trinitrophenol has been established. The method shows good sensitivity and accuracy in real water samples, providing a feasible way to avoid the threat of contaminated drinking water.
Article
Chemistry, Multidisciplinary
Mingkai Wang, Hanlin Wei, Shuai Wang, Chuanyu Hu, Qianqian Su
Summary: This study focused on developing lanthanide-doped nanocrystals with efficient ultraviolet emission for applications in drug release, photocatalysis, photodynamic therapy, and solid-state lasing. By using a dye-sensitized, heterogeneous core-multishelled lanthanide nanoparticle, the researchers were able to significantly enhance ultraviolet upconversion. Their findings demonstrated a promoted multiphoton upconversion and provided insights into the energy transfer pathway for future developments in ultraviolet-emissive nanohybrids.
Article
Spectroscopy
Shuaibing Yu, Yan Li, Lei Gao, Peiran Zhao, Lei Wang, Lianzhi Li, Ying-Wu Lin
Summary: A novel ratiometric fluorescent sensor for Zn2+ was developed based on a zinc finger-like peptide, showing highly selective and sensitive response with a low detection limit and low biotoxicity.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Multidisciplinary
Fei Zhao, Jialing Hu, Daoming Guan, Jinyang Liu, Xuebo Zhang, Huan Ling, Yunxiang Zhang, Qian Liu
Summary: This study proposes a new strategy to enhance the efficiency of dye-triplet-sensitized upconversion emission by modifying the coordination sites on the conjugated structure of cyanine molecules. The approach improves the energy transfer efficiency between organic dyes and lanthanide ions Yb3+ and can be applied to both 808 nm and 730 nm dye-sensitization systems. The strategy also demonstrates enhancement of upconversion at the single particle level through dye-sensitization.
ADVANCED MATERIALS
(2023)
Article
Spectroscopy
Yongpeng Wang, Jia Chen, Yang Shu, Jianhua Wang, Hongdeng Qiu
Summary: In this study, a fluorescent probe TNP-SBD-Cl was developed to specifically identify cysteine (Cys) and avoid interference from other biological analytes. The probe can monitor Cys in living HeLa cells with low cytotoxicity, showing significant diagnostic potential in physiological and pathological processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Chemistry, Multidisciplinary
Steven C. Boggess, Shivaani S. Gandhi, Brittany R. Benlian, Evan W. Miller
Summary: Improvements in the fluorene molecular wire scaffold by adding a single vinyl spacer led to enhanced voltage sensitivity and reduced phototoxicity, enabling successful monitoring of action potential kinetics in various cell types.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Physical
Tesla Yudhistira, Elisabete Cruz Da Silva, Antoine Combes, Maxime Lehmann, Andreas Reisch, Andrey S. S. Klymchenko
Summary: This study reports a method of antibody labeling using biotinylated zwitterionic dye-loaded polymeric nanoparticles. The prepared biotinylated nanoparticles are small (14 nm) and bright, capable of carrying a large amount of cationic rhodamine dye. The method significantly improves fluorescence imaging of target epidermal growth factor receptors and becomes a useful tool in the high-sensitivity detection of disease biomarkers.
Article
Chemistry, Physical
S. Irudhaya Raj, Adhish Jaiswal
Summary: In this study, covellite copper sulfide nanoparticles were synthesized as a Fenton-like catalyst through a simple precipitation method. The CuS catalyst showed degradation of cationic dyes in dark conditions and both cationic and anionic dyes in visible light, with an enhanced degradation rate under visible light. Analysis using XRD, UV-Vis-NIR, and XPS spectra revealed structural transformations of the CuS catalyst during the Fenton reaction under different conditions.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2021)
Article
Nanoscience & Nanotechnology
Simranjit Singh, Vishakha Takhar, Shivanand H. Nannuri, Sajan D. George, Rupak Banerjee, Superb K. Misra
Summary: The synergistic effect of synthesized MoS2 nanosheets and NaYF4:Yb,Tm,Nd upconversion nanoparticles (UCNP) on effective dye degradation is demonstrated. The optical properties show that the emission spectra of UCNP overlap with the absorption spectra of MoS2. The combination of UCNP and syn-MoS2 achieves 96% degradation of methylene blue, compared to 56.4% for syn-MoS2 alone and 42% for b-MoS2. Concentration-dependent dye degradation studies reveal a maximum removal efficiency of 99.6% with 150 mg L-1 of syn-MoS2 and 200 mg L-1 of UCNP within 2 hours.
ACS APPLIED NANO MATERIALS
(2023)
Article
Biophysics
Xianghan Zhang, Xiaohan Gao, Jialin Zhou, Zhiqing Gao, Yingdi Tang, Zuhong Tian, Pengbo Ning, Yuqiong Xia
Summary: The albumin-based FRET nanoprobe Cy5/7@HSA NPs showed high sensitivity and excellent imaging in cell and tumor imaging, indicating its strong potential as a multileveled tumor imaging platform.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Multidisciplinary Sciences
Chungwon Park, Xingxiang Chen, Chong-Li Tian, Gyu Nam Park, Nicolas Chenouard, Hunki Lee, Xin Yi Yeo, Sangyong Jung, Richard W. Tsien, Guo-Qiang Bi, Hyokeun Park
Summary: Maintaining the balance between excitatory and inhibitory synaptic transmission is crucial for proper central nervous system function. Inhibitory synaptic vesicles exhibit unique dynamics and fusion properties that support rapid synaptic inhibition, showing differences compared to excitatory synaptic vesicles labeled with synaptotagmin-1. This study provides insights into the specialized mechanisms of inhibitory synaptic vesicle release, contributing to the understanding of coordinated excitation and inhibition in the CNS.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Neurosciences
Mei-Yu Shi, Lu-Feng Ding, Yu-Hong Guo, Yu-Xiao Cheng, Guo-Qiang Bi, Pak-Ming Lau
Summary: The GAD2 inhibitory neurons in the NTS have both local and short-range projections, as well as previously unknown long-range projections targeting forebrain regions. These findings provide new insights into the role of NTS in physiological and emotional modulation.
Article
Ethics
Sara Goering, Eran Klein, Laura Specker Sullivan, Anna Wexler, Blaise Aguera y Arcas, Guoqiang Bi, Jose M. Carmena, Joseph J. Fins, Phoebe Friesen, Jack Gallant, Jane E. Huggins, Philipp Kellmeyer, Adam Marblestone, Christine Mitchell, Erik Parens, Michelle Pham, Alan Rubel, Norihiro Sadato, Mina Teicher, David Wasserman, Meredith Whittaker, Jonathan Wolpaw, Rafael Yuste
Summary: The advancement of novel neurotechnologies has profound implications for society and individual rights, potentially challenging basic human concepts. To mitigate negative consequences, recommendations include establishing global ethical guidelines, enhancing data privacy protection, preventing bias, and ensuring safe and equitable distribution of neurotechnological devices.
Article
Biotechnology & Applied Microbiology
Fang Xu, Yan Shen, Lufeng Ding, Chao-Yu Yang, Heng Tan, Hao Wang, Qingyuan Zhu, Rui Xu, Fengyi Wu, Yanyang Xiao, Cheng Xu, Qianwei Li, Peng Su, Li I. Zhang, Hong-Wei Dong, Robert Desimone, Fuqiang Xu, Xintian Hu, Pak-Ming Lau, Guo-Qiang Bi
Summary: This study introduces a new method combining primate-optimized tissue sectioning and clearing with high-speed fluorescence microscopy to achieve high-resolution imaging of the entire rhesus monkey brain. Researchers have also developed an efficient method for tracing sparse axonal fibers in large datasets, enabling connectome-scale mapping of large primate brains.
NATURE BIOTECHNOLOGY
(2021)
Article
Engineering, Biomedical
Shengying Fan, Lei Qi, Jiawen Li, Deng Pan, Yiyuan Zhang, Rui Li, Cong Zhang, Dong Wu, Pakming Lau, Yanlei Hu, Guoqiang Bi, Weiping Ding, Jiaru Chu
Summary: The patterning of axonal and dendritic growth is crucial for generating directed neuronal networks in vitro. 3D micropillar scaffolds can guide the growth of neural stem cells and hippocampal neurons, affecting branching and orientation of axons and dendrites. Neurons tend to form functionally connected networks on arrays of micropillars of the same height, showing synchronous neuronal activity.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Biochemical Research Methods
Lei Qu, Yuanyuan Li, Peng Xie, Lijuan Liu, Yimin Wang, Jun Wu, Yu Liu, Tao Wang, Longfei Li, Kaixuan Guo, Wan Wan, Lei Ouyang, Feng Xiong, Anna C. Kolstad, Zhuhao Wu, Fang Xu, Yefeng Zheng, Hui Gong, Qingming Luo, Guoqiang Bi, Hongwei Dong, Michael Hawrylycz, Hongkui Zeng, Hanchuan Peng
Summary: The study introduces a cross-modal registration method, mBrainAligner, for aligning whole mouse brain images to the standard Allen Common Coordinate Framework atlas. Researchers then built a mouse brain atlas for the fluorescence micro-optical sectioning tomography modality, enabling further research on single-cell mapping and neuron types.
Article
Biology
Chuan Huang, Yu Wang, Peng Chen, Qing-Hong Shan, Hao Wang, Lu-Feng Ding, Guo-Qiang Bi, Jiang-Ning Zhou
Summary: This study used a rabies-virus system to map the single-cell input onto corticotropin-releasing factor (CRF) neurons in the central amygdala (CeA). The inputs were found to be located in 44 nested subregions, with most of them being top-down convergent inputs from the cortex, especially in layer 4 of the somatosensory cortex, and relaying information from the thalamus. The bottom-up divergent inputs had a higher expression of glutamate decarboxylase. The study also revealed the en passant structures of single input neurons through in-situ reconstruction. These findings provide valuable insights into the circuit mechanisms of CeA-CRF neurons.
COMMUNICATIONS BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhaohuan Huang, Ling Liu, Jian Zhang, Kristie Conde, Jay Phansalkar, Zhongzhong Li, Lei Yao, Zihui Xu, Wei Wang, Jiangning Zhou, Guoqiang Bi, Feng Wu, Randy J. Seeley, Michael M. Scott, Cheng Zhan, Zhiping P. Pang, Ji Liu
Summary: This study identified a population of glucose-sensing GLP-1 receptor neurons in the dorsomedial hypothalamic nucleus, which can decrease blood glucose levels through communication with the pancreas. The mechanism by which this central GLP-1 signal controls glucose levels was also elucidated.
Article
Multidisciplinary Sciences
Yun-Tao Liu, Heng Zhang, Hui Wang, Chang-Lu Tao, Guo-Qiang Bi, Z. Hong Zhou
Summary: IsoNet is a neural network that addresses the issue of anisotropic resolution in cryogenic electron tomography by learning and recovering feature representations of similar structures in the tomogram. Its application greatly improves the interpretability and visualization of cellular structures.
NATURE COMMUNICATIONS
(2022)
Article
Neurosciences
Qiao-Qiong Liu, Yu-Xiao Cheng, Qi Jing, Ke-Ming Zhang, Lu-Feng Ding, Xiao-Wei Fan, Chun-Hui Jia, Fang Xu, Guo-Qiang Bi, Pak-Ming Lau
Summary: This study employed a dual-viral injection strategy to label cortical pyramidal neurons targeting the pedunculopontine nucleus (PPN) in mice. By using a high-speed volumetric imaging technique, the researchers visualized the brain-wide axonal projections of these neurons from various cortical areas and identified unique collateralization patterns to different subcortical areas. The findings provide potential anatomical mechanisms for the coordination between the PPN and other subcortical areas in performing different physiological functions.
Article
Neurosciences
Xiao-Wei Li, Yi Ren, Dong-Qing Shi, Lei Qi, Fang Xu, Yanyang Xiao, Pak-Ming Lau, Guo-Qiang Bi
Summary: Acetylcholine (ACh) affects neural circuit dynamics by inhibiting excitatory synaptic transmission and enhancing neuronal excitability through muscarinic signaling pathways. It suppresses evoked reverberation at low to moderate doses, while high doses result in longer duration of reverberation and increased spontaneous activity. These effects are mediated by muscarinic, but not nicotinic receptors, and can be simulated through changes in excitatory postsynaptic currents (EPSCs) and the excitability of single cells.
NEUROSCIENCE BULLETIN
(2023)
Letter
Neurosciences
Dong-Qing Shi, Fang Xu, Guo-Qiang Bi, Pak-Ming Lau
NEUROSCIENCE BULLETIN
(2023)
Article
Neurosciences
Ke-Ming Zhang, Yan Shen, Chun-Hui Jia, Hao Wang, Guo-Qiang Bi, Pak-Ming Lau
Summary: Cooperation is a vital social behavior for survival, and a new paradigm was developed to train and test cooperative behavior in mice. The study found extensive social contact and waiting behavior, which were positively correlated with the success of cooperation. Using c-Fos immunostaining and high-speed volumetric imaging, the researchers identified increased neuronal activity in cortical areas during cooperation, providing insights into the underlying neural circuitry mechanisms.
Article
Neurosciences
Wantong Shi, Man Xue, Fengyi Wu, Kexin Fan, Qi-Yu Chen, Fang Xu, Xu-Hui Li, Guo-Qiang Bi, Jing-Shan Lu, Min Zhuo
Summary: This study examined the axonal outputs of the anterior cingulate cortex (ACC) in the whole brain of adult male mice. The ACC primarily projected ipsilaterally to several regions such as the caudate putamen, ventral thalamic nucleus, zona incerta, periaqueductal gray, superior colliculus, interpolar spinal trigeminal nucleus, and dorsal medullary reticular nucleus. The ACC also projected to contralateral brain regions. These findings provide a whole-brain mapping of the ACC projections in adult male mice and are important for future studies on ACC-related brain diseases.
Article
Neurosciences
Man Xue, Wan-Tong Shi, Si-Bo Zhou, Ya-Nan Li, Feng-Yi Wu, Qi-Yu Chen, Ren-Hao Liu, Zhao-Xiang Zhou, Yu-Xiang Zhang, Yu-Xin Chen, Fang Xu, Guo-Qiang Bi, Xu-Hui Li, Jing-Shan Lu, Min Zhuo
Summary: The anterior cingulate cortex (ACC) plays important roles in pain perception and emotion, with the thalamocortical pathway being the major sensory input. Adult mice, especially genetically modified mice, provide important mechanisms for cortical plasticity and modulation in the ACC. Research found that cortical neurons in the ACC received direct projections from different sub-nuclei in the thalamus.
