Article
Biophysics
Mirjana Petronijevic, Sanja Panic, Sasa Savic, Jasmina Agbaba, Jelena Molnar Jazic, Marija Milanovic, Natasa Durisic-Mladenovic
Summary: This study investigates the use of wood-derived biochar as a support for the covalent immobilization of horseradish peroxidase for phenol removal in wastewater treatment. The immobilized enzyme showed high efficiency in phenol removal under specific reaction conditions, and significantly reduced the toxicity of the treated water.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2021)
Article
Biochemistry & Molecular Biology
Friederike Dehli, Cosima Stubenrauch, Alexander Southan
Summary: This study demonstrates the potential of biotinylated gelatin methacryloyl in the preparation of hydrogel foams and the successful control of substrate conversion rate by altering the hydrogel morphology.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Environmental Sciences
Umme Kalsoom, Nasira Khalid, Affaf Ibrahim, Syed Salman Ashraf, Haq Nawaz Bhatti, Zainab Ahsan, Jakub Zdarta, Muhammad Bilal
Summary: This study investigates the biodegradation of direct blue (DB) 297 and reactive blue (RB) 221 using immobilized horseradish peroxidase (HRP) on iron oxide nanoparticles. The nanoparticles were synthesized by co-precipitation and showed an immobilization efficiency of 87%. The immobilized HRP exhibited higher thermal stability, pH stability, and dye degradation efficiency compared to free HRP.
Article
Engineering, Environmental
Jiacong Wu, Xinnan Ma, Chunmei Li, Xiangtong Zhou, Juan Han, Lei Wang, Hongjun Dong, Yun Wang
Summary: A novel photon-enzyme cascade catalysis system, composed of hybrid HRP-CN/Cu-3(PO4)(2) nanoflowers, was successfully constructed. This system exhibits excellent immobilized enzyme enzymatic properties and better tolerance to extreme conditions compared to free HRP. It shows outstanding degradation performance for bisphenol A (BPA) and can replace traditional systems for the harmless treatment of BPA in wastewater.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Chemistry, Analytical
Silvina V. Kergaravat, Silvia N. Fabiano, Adriana R. Soutullo, Silvia R. Hernandez
Summary: Glyphosate is the most widely used herbicide in Argentina, and there is controversy surrounding its safety. Two electrochemical screening methods based on horseradish peroxidase (HRP) inhibition have been developed for glyphosate detection.
MICROCHEMICAL JOURNAL
(2021)
Article
Biochemistry & Molecular Biology
Malgorzata Latos-Brozio, Anna Masek, Malgorzata Piotrowska
Summary: This study successfully achieved enzymatic polymerization of naringenin and investigated the properties of poly(naringenin). It was found that the polymeric forms of naringenin exhibited improved thermal stability, antioxidant activity, and antimicrobial properties compared to the basic flavonoid. In particular, poly(naringenin)-laccase showed better scavenging activity for ABTS(+center dot) radicals and antimicrobial activity against E. coli.
Article
Engineering, Environmental
Haitao Li, Linfeng Guo, Yingying Li, Min Chen, Chunlu Bai, Aolei Song, Linxiu Cheng, Xueli Chen, Yonglin Chen
Summary: This paper investigated the degradation of BPA in synthetic wastewater using the immobilization of horseradish peroxidase membrane reactor. The immobilized HRP microporous membrane showed good activity and stability. By optimizing the reaction parameters, a high BPA degradation efficiency of about 78% was achieved. This study is of great significance for addressing the environmental issues associated with BPA discharge.
WATER SCIENCE AND TECHNOLOGY
(2023)
Article
Engineering, Chemical
Jiacong Wu, Xinnan Ma, Ting He, Juan Han, Yan Zhu, Chunmei Li, Yun Wang
Summary: The structural limitation of the photocatalyst itself hinders the increase in enzyme loading amount in the photo-enzyme coupling system. A g-C3N4-based photocatalyst ACN(45) with high enzyme loading amount was synthesized to address this issue. The channels in ACN(45) matched well with the size of the enzyme molecules, enabling a loading amount 6.3 times higher than pure g-C3N4. The ACN(45) channels also improved enzyme activity and stability, and reduced enzyme leaching. The high loading amount of the photo-enzyme coupling system enhanced the photocatalytic degradation of BPA, achieving a degradation rate 5.9 times higher than pure g-C3N4 within 1 hour. These findings provide a simple approach to design a photo-enzyme coupling system with high enzyme loading and stability.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jakub Zdarta, Oliwia Degorska, Katarzyna Jankowska, Agnieszka Rybarczyk, Adam Piasecki, Filip Ciesielczyk, Teofil Jesionowski
Summary: Immobilization of horseradish peroxidase in sodium alginate capsules and further trapping in poly(vinyl chloride) electrospun fibers enhances enzyme stability, reusability, catalytic properties, and substrate affinity, making it a promising tool for enzymatic conversion of pharmaceutically active ingredients.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Engineering, Chemical
Gulay Bayramoglu, Aydin Akbulut, Mehmet Yakup Arica
Summary: HRP was successfully immobilized on a gel for the degradation of Direct Blue-6 dye, with significant results. The loading amount and activity of HRP were evaluated, along with toxicity testing and algal growth studies, showing a significant decrease in toxicity of the degradation products after enzyme treatment.
CHEMICAL ENGINEERING RESEARCH & DESIGN
(2021)
Article
Chemistry, Multidisciplinary
Anu Maria Chittilappilly Devassy, Adithya Kamalakshan, Nidhi Anilkumar Jamuna, Roselin Ansilda, Sarthak Mandal
Summary: The immobilization of enzymes on functional nanomaterials to form nanobiohybrids offers numerous research opportunities. In this study, a hybrid nanobiocatalytic system was formed through the adsorption of cytochrome c on stabilized MoS2 nanosheets. The nanobiointeractions between the two components enhanced the catalytic activity of the hybrid, making it a novel nanobiocatalyst with superior activity.
Article
Engineering, Environmental
Neda Mashhadi, Keith E. Taylor, Nihar Biswas, Paul Meister, James W. Gauld
Summary: Azoles are important small molecules found in pharmaceuticals and pesticides that are now considered emerging contaminants. Peroxidases have been shown to effectively transform these compounds, with enzymatic treatment achieving a transformation efficiency of >= 70% in all cases. Mass spectrometry analysis revealed the formation of dimers and trimers as radical-coupling products after treatment, with structures proposed based on predicted regiochemistry.
ENVIRONMENTAL SCIENCE-WATER RESEARCH & TECHNOLOGY
(2021)
Article
Engineering, Environmental
Nevena Pantic, Milica Spasojevic, Zeljko Stojanovic, Dorde Veljovic, Jugoslav Krstic, Ana Marija Balaz, Radivoje Prodanovic, Olivera Prodanovic
Summary: Novel macroporous copolymers with mean pore size diameters ranging from 150 to 310 nm were synthesized and modified with ethylenediamine for horseradish peroxidase (HRP) immobilization. The activity and stability of immobilized HRP were greatly affected by the pore size of the carrier, and the highest specific activities were obtained for carriers with pore size diameters of 234 and 297 nm. HRP immobilized on the copolymer with a pore size of 234 nm showed higher activities than the native enzyme and exhibited good stability.
JOURNAL OF POLYMERS AND THE ENVIRONMENT
(2022)
Article
Environmental Sciences
Jing-Jing Liu, Jong-Gook Kim, Hye-Bin Kim, Shakya Abeysinghe, Ying-Wu Lin, Kitae Baek
Summary: Enzyme-based biocatalytic treatment is an effective method for degrading organic pollutants. In this study, horseradish peroxidase (HRP) was immobilized on functionalized rice straw biochar using glutaraldehyde as a crosslinker. The biochar was pretreated by electrochemical and acid treatments to enhance its immobilizing ability.
Article
Nanoscience & Nanotechnology
Gema Vivo-Llorca, Angela Morella-Aucejo, Alba Garcia-Fernandez, Paula Diez, Antoni Llopis-Lorente, Mar Orzaez, Ramon Martinez-Manez
Summary: In this study, a novel nanodevice based on enzyme-functionalized gold nanoparticles for enzyme prodrug therapy (EPT) in breast cancer was designed and evaluated. The results showed that the nanodevice was effectively internalized by breast cancer cells and co-treatment with the nanodevice and enzyme prodrug significantly reduced the viability of breast cancer cells. The findings from three-dimensional tumor models also demonstrated the therapeutic effectiveness of this nanodevice.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Minoli Aponso, Milton T. W. Hearn, Antonio F. Patti, Louise E. Bennett
Summary: The study compared the neuroactivity of essential oils (EOs) and reference pharmaceutical compounds on the brain, revealing that EOs can replicate specific neurotransmitter receptor binding patterns, affecting EEG-alpha wave frequencies.
ACS CHEMICAL NEUROSCIENCE
(2022)
Article
Chemistry, Applied
Poornima Vijay, Warren Batchelor, Kei Saito
Summary: This research explains a one-pot process using iron oxide catalysts and hydrogen peroxide to fibrillate cellulose and obtain nanocellulose fibers and water-soluble oxidized cellulose. By controlling factors such as the valency and particle size of the catalyst, as well as the reaction time, high yields of nanocellulose fibers (83%) and water-soluble oxidized cellulose (38%) were achieved. The nanocellulose fibers had a crystallinity of 73%, a degree of polymerization (DP) of 150, and dimensions of 30-110 nm thickness and 1-5 μm length. The water-soluble product had a carboxyl content of 2.9 mmol/g and DP of 25. This research provides an alternative method that eliminates the need for halogenated reagents, strong acids, and mechanical pretreatments.
CARBOHYDRATE POLYMERS
(2022)
Article
Polymer Science
Karteek Boga, Antonio F. Patti, John C. Warner, George P. Simon, Kei Saito
Summary: This research reports a new environmentally-friendly light-assisted polymerization method for the synthesis of highly cross-linked polymer microspheres, and the results show the formation of highly crosslinked porous microspheres with good thermal stability.
MACROMOLECULAR CHEMISTRY AND PHYSICS
(2022)
Article
Polymer Science
Ke Xu, Bo Fan, Kevin Putera, Michaela Wawryk, Jing Wan, Bo Peng, Mark M. Banaszak Holl, Antonio F. Patti, San H. Thang
Summary: This study demonstrates a new surfactant-free emulsion polymerization method, using surface cross-linking technology to achieve high cross-linking efficiency and prevent surfactant migration. This approach produces films with improved physical properties.
Article
Polymer Science
Ruchi Pal, Arun K. Sikder, Kei Saito, Alison M. Funston, Jayesh R. Bellare
Summary: Polymer blends are important for both commercial utility and scientific understanding, and the degree of interfacial mixing in these blends influences their mechanical properties. This paper introduces a method using scanning transmission electron microscopy spectrum imaging to calculate the interfacial width in immiscible polymer blends and demonstrates its application using a polycarbonate-polystyrene bilayer sample as an example.
POLYMER INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Zongsheng Zou, Tayyaba Younas, Geoff Dumsday, Victoria S. Haritos, Lizhong He
Summary: RNA aptamers have the ability to specifically and selectively bind to various macromolecules, cell surfaces, and viruses, and find wide applications in biosensors, diagnostics, and therapeutic treatments and drug delivery. This article presents a new E. coli-based approach for rapidly producing multimeric RNA aptamer transcripts in vivo, using a designed double-stranded spacer to protect the transcript ends. The produced multimeric and fluorescent RNA aptamers were stable and easily isolated, and their full functionality was confirmed through binding assays and fluorescence measurements. This approach shows promise for rapid and cost-effective production of RNA aptamers.
BIOTECHNOLOGY JOURNAL
(2023)
Article
Polymer Science
Pallabi Sinha Roy, Matthieu M. Mention, Antonio F. Patti, Gil Garnier, Florent Allais, Kei Saito
Summary: A novel and highly tunable reversible polymer adhesive was designed using lignin, the second most abundant polymer. The adhesive exhibits reversible adhesion switching through the photo-responsive property of the p-hydroxycinnamic acid structure. The design strategy and structure-property analysis mechanisms described in this work can be applied to engineer new bio-based and reusable adhesives.
Article
Chemistry, Multidisciplinary
Tayyaba Younas, Chang Liu, Weston B. Struwe, Philipp Kukura, Lizhong He
Summary: Researchers have engineered light-responsive RNA-protein nanowires that exhibit non-covalent light-switching ability. By utilizing the high affinity between RNA and protein, they replaced green fluorescence protein with an optogenetic protein pair system, allowing blue light-controlled photo-switching. These RNA-protein structures can serve as biocompatible frameworks for incorporating versatile elements such as RNA, DNA, and enzymes.
Article
Chemistry, Multidisciplinary
Christian K. O. Dzuvor, Bhuvana K. Shanbhag, Hsin-Hui Shen, Victoria S. Haritos, Lizhong He
Summary: Protein-based nanomaterials have outstanding properties and wide applications in biomedical and bionanotechnological sectors. However, the development of unique nanostructures for next-generation antibacterial agents has been limited. This study reports the discovery of protein nanospears, which are supramolecular nanostructures with well-defined shapes based on engineered proteins. The nanospears exhibit exceptional antibacterial activities, rapidly damaging bacterial morphology via their nanostructure and enzymatic action. These protein-based nanospears show promise as potent tools to combat drug-resistant bacteria and inspire the engineering of other antibacterial protein nanomaterials with diverse properties.
ADVANCED MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Karteek Boga, Antonio F. Patti, John C. Warner, George P. Simon, Kei Saito
Summary: The increasing demand for high-performance thermosetting adhesives has led to the depletion of fossil reserves and environmental pollution by recalcitrant plastic waste. In this work, sustainable green adhesives were developed by combining bio-renewable epoxidized soybean oil and non-toxic coumarins, which can undergo reversible cycloaddition reaction under UV light. The synthesized polymer networks showed excellent adhesion strength and reusability.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Moeka Inada, Tatsuhiro Horii, Toshinori Fujie, Takuya Nakanishi, Toru Asahi, Kei Saito
Summary: Debonding-on-demand (DoD) adhesives, with the ability to repeatedly adhere and release in response to external stimuli, are attracting attention as sustainable functional materials. This study presents a DoD adhesive system based on a polymer fabricated by the reversible cycloaddition reaction of coumarin-terminated four-arm siloxane monomers. The thermophysical property analysis confirms that photoinduced fluidization is achieved through reversible crosslinking and decrosslinking reactions of the monomers. This simple siloxane-based reversible cycloaddition system exhibits significant potential as a room temperature DoD adhesive.
MATERIALS ADVANCES
(2023)
Article
Agriculture, Multidisciplinary
Ruth M. Barajas-Ledesma, Craig W. Stocker, Vanessa N. L. Wong, Karen Little, Antonio F. Patti, Gil Garnier
Summary: In this study, the effects of different soil moisture levels and types of superabsorbent polymers (SAPs) on spinach plants were examined. The highest plant biomass was observed in sandy soil amended with a commercial SAP. However, the addition of a SAP decreased plant biomass in clay soil, regardless of the type. The use of nanocellulose SAP was associated with waterlogging stress and rapid biodegradation. Further research is needed to modify the structure of nanocellulose SAP for improved agricultural benefits.
ACS AGRICULTURAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Moeka Inada, Akihiro Udagawa, Sota Sato, Toru Asahi, Kei Saito
Summary: This study investigates the conversion of self-assembled structures into continuous polymeric structures using the [2 + 2]-cycloaddition reaction. Bio-inspired thymine-based bolaamphiphilic molecules were designed and synthesized to allow cycloaddition reaction between two thymines in their self-assembled structure. The study provides a strategy for creating higher molecular weight linear polymers by controlling the photocyclization sites within the self-assembly.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2022)
Article
Engineering, Chemical
Shivali Banerjee, Meghana Munagala, Yogendra Shastri, Ranganathan Vijayaraghavan, Antonio F. Patti, Amit Arora
Summary: This study assesses the techno-economic feasibility of an integrated biorefinery based on pineapple processing waste and identifies increased plant capacity and selling price of value-added products as the crucial factors for improving plant economics.
ACS ENGINEERING AU
(2022)
