Article
Immunology
Elena Putscher, Michael Hecker, Brit Fitzner, Nina Boxberger, Margit Schwartz, Dirk Koczan, Peter Lorenz, Uwe Klaus Zettl
Summary: This study found that genetic variants from MS risk loci affect pre-mRNA splicing. These findings substantiate the role of alternative splicing events in the genetics of MS. Further research on how disease-causing genetic variants modify the interactions between splicing regulatory sequence elements and RNA-binding proteins can help deepen our understanding of the genetic susceptibility to MS.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Oncology
Sunkyung Choi, Namjoon Cho, Eun-Mi Kim, Kee K. Kim
Summary: Alternative pre-mRNA splicing plays a critical role in generating multiple mRNA and increasing protein diversity. Specific splicing isoforms have been found to be important in cellular processes, particularly in regulating cell numbers. Abnormal expression of splicing isoforms and factors, as well as disruptions in splicing caused by genetic mutations, are implicated in cancer development and progression. Alternative splicing has potential as a therapeutic target for cancer.
CANCER CELL INTERNATIONAL
(2023)
Article
Multidisciplinary Sciences
Yang Pan, John W. Phillips, Beatrice D. Zhang, Miyako Noguchi, Eric Kutschera, Jami McLaughlin, Pavlo A. Nesterenko, Zhiyuan Mao, Nathanael J. Bangayan, Robert Wang, Wendy Tran, Harry T. Yang, Yuanyuan Wang, Yang Xu, Matthew B. Obusan, Donghui Cheng, Alex H. Lee, Kathryn E. Kadash-Edmondson, Ameya Champhekar, Cristina Puig-Saus, Antoni Ribas, Robert M. Prins, Christopher S. Seet, Gay M. Crooks, Owen N. Witte, Yi Xing
Summary: This study describes a computational platform called IRIS that can discover tumor antigens derived from alternative splicing, providing potential targets for TCR and CAR-T immunotherapies. Through the analysis of transcriptomics and immuno-peptidomics data, the study demonstrates that the predicted targets by IRIS can bind with HLA molecules. The study illustrates the contribution of alternative splicing to the repertoire of tumor antigens and demonstrates the utility of IRIS in discovering AS-derived antigens and expanding cancer immunotherapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Yulia A. Gladilina, Lylia Bey, Abdullah Hilal, Ekaterina V. Neborak, Varvara G. Blinova, Dmitry D. Zhdanov
Summary: This study highlights the cytoprotective effects of polyamines on normal human CD4(+) T lymphocytes through alternative splicing of RAD51A pre-mRNA, leading to decreased DNA damage and increased cell survival. However, these effects were not observed in cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Huihui Yu, Qian Du, Malachy Campbell, Bin Yu, Harkamal Walia, Chi Zhang
Summary: Pre-mRNA splicing is crucial for gene expression regulation. Researchers developed a software tool called VaSP to quantify and visualize splicing variants in plants, and identified genotype-specific splicing events associated with shoot Na+ accumulation under salt stress conditions. VaSP proves to be a versatile and powerful tool for alternative splicing analysis and prioritizing candidate causal pre-mRNA splicing in GWAS.
Review
Genetics & Heredity
Zhongjing Su, Dongyang Huang
Summary: Alternative splicing plays a critical role in the regulation of immune cell development and immune activity, with aberrant splicing leading to abnormal immune responses. This review summarizes recent discoveries on immune-associated alternative splice variants, clinical disorders, and regulatory mechanisms.
Article
Cell Biology
Yanfei Wang, Cuiqiao Zhang, Wu Peng, Haibo Du, Yuehui Xi, Zhigang Xu
Summary: This study further explored the role of RBM24 in hearing and balance through conditional knockout mice and found that the loss of RBM24 results in severe deficits. It was observed that the development of hair cells was significantly affected, leading to degeneration and disorganization of the hair bundles. RNAseq analysis also identified several genes whose mRNA stability or pre-mRNA alternative splicing is affected by RBM24 knockout.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Mingtian Deng, Baobao Chen, Zifei Liu, Yongjie Wan, Dongxu Li, Yingnan Yang, Feng Wang
Summary: YBX1 plays important roles in maternal mRNA decay, alternative splicing, and transcriptional activity during early embryogenesis. These findings broaden our understanding of YBX1 functions.
CELL AND BIOSCIENCE
(2022)
Review
Plant Sciences
Praveen Kumar Kathare, Enamul Huq
Summary: The article summarizes the role of phytochromes in regulating plant growth and development, particularly in the regulation of pre-mRNA splicing process.
CURRENT OPINION IN PLANT BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Jingya Lin, Ziqiang Zhu
Summary: This review discusses how plants adapt to high temperatures through pre-mRNA splicing, and the significance of this splicing regulation for plant growth and development.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Baohua Tan, Jiekang Zeng, Fanming Meng, Shanshan Wang, Liyao Xiao, Xinming Zhao, Linjun Hong, Enqin Zheng, Zhenfang Wu, Zicong Li, Ting Gu
Summary: This study suggests that m6A modification plays an important role in mediating the phenotypic differences between oxidative and glycolytic muscles through the regulation of alternative splicing (AS), providing novel insights into the mechanisms underlying muscle fiber conversion.
Review
Immunology
Frank Fang Yao Lee, Scott Alper
Summary: While TLR signaling is necessary to combat infection, persistent inflammation can cause damage and contribute to various inflammatory disorders. Alternative pre-mRNA splicing plays a role in terminating TLR signaling by producing dominant negative inhibitors that limit inflammation. Understanding the production and function of these alternative isoforms may provide insights into inflammatory disease.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wing-Keung Chu, Li-Man Hung, Chun-Wei Hou, Jan-Kan Chen
Summary: The Yes-associated protein (YAP) is a transcriptional co-activator crucial for organ development and tumorigenesis, inhibited by the Hippo signaling pathway. In human cancer cells, alternative splicing of YAP 3' UTR generates a shorter mRNA with potential impact on YAP expression. PKC inhibitor affects the ratio of different YAP mRNA forms, implicating PKC in the regulation of YAP expression through alternative splicing.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
James D. D. Fackenthal
Summary: Cancer is a leading cause of death worldwide. Alternative mRNA splicing has recently been recognized as a major contributor to cancer initiation and progression. Cancer cells exhibit different distribution of splicing variants compared to non-cancer cells, and they are more sensitive to drugs targeting the splicing regulatory network. Certain recurring splicing variants have been shown to contribute to tumor progression, while some disruption events result from mutations in splicing regulatory sequences or shifts in balance among naturally occurring splicing variants of mRNAs involved in cell cycle progression and apoptosis regulation. This review explores cancer-related alternative splicing events and potential therapies.
Review
Plant Sciences
Wei Lan, Yuhao Qiu, Yun Xu, Yalin Liu, Ying Miao
Summary: Alternative splicing plays a critical role in plant development and response to environmental stress. Post-translational modification, such as protein ubiquitination, has been found to regulate the activity and stability of spliceosomal proteins, providing another layer of regulation for alternative splicing. This review summarizes recent studies on ubiquitin and ubiquitin-like modification of spliceosome components and discusses the relationship between spliceosome and the ubiquitin/26S proteasome pathway in plants.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Shayna Stein, Zhi-xiang Lu, Emad Bahrami-Samani, Juw Won Park, Yi Xing
NUCLEIC ACIDS RESEARCH
(2015)
Editorial Material
Biotechnology & Applied Microbiology
Leng Han, Yan Guo, Zhixi Su, Siyuan Zheng, Zhixiang Lu
BIOMED RESEARCH INTERNATIONAL
(2015)
Article
Biochemical Research Methods
Benoit Molinie, Jinkai Wang, Kok Seong Lim, Roman Hillebrand, Zhi-xiang Lu, Nicholas Van Wittenberghe, Benjamin D. Howard, Kaveh Daneshvar, Alan C. Mullen, Peter Dedon, Yi Xing, Cosmas C. Giallourakis
Article
Biochemistry & Molecular Biology
Rui Zhou, Juw Won Park, Rene F. Chun, Thomas S. Lisse, Alejandro J. Garcia, Kathryn Zavala, Jessica L. Sea, Zhi-xiang Lu, Jianzhong Xu, John S. Adams, Yi Xing, Martin Hewison
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biotechnology & Applied Microbiology
Jinsil Kim, Keyan Zhao, Peng Jiang, Zhi-xiang Lu, Jinkai Wang, Jeffrey C. Murray, Yi Xing
Article
Biochemistry & Molecular Biology
Jinkai Wang, Zhi-xiang Lu, Collin J. Tokheim, Sara E. Miller, Yi Xing
MOLECULAR BIOLOGY AND EVOLUTION
(2015)
Article
Oncology
Zhi-xiang Lu, Qin Huang, Juw Won Park, Shihao Shen, Lan Lin, Collin J. Tokheim, Michael D. Henry, Yi Xing
MOLECULAR CANCER RESEARCH
(2015)
Article
Biochemistry & Molecular Biology
Shihao Shen, Juw Won Park, Jian Huang, Kimberly A. Dittmar, Zhi-xiang Lu, Qing Zhou, Russ P. Carstens, Yi Xing
NUCLEIC ACIDS RESEARCH
(2012)
Article
Multidisciplinary Sciences
Shihao Shen, Juw Won Park, Zhi-xiang Lu, Lan Lin, Michael D. Henry, Ying Nian Wu, Qing Zhou, Yi Xing
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2014)
Article
Biotechnology & Applied Microbiology
Keyan Zhao, Zhi-xiang Lu, Juw Won Park, Qing Zhou, Yi Xing
Editorial Material
Genetics & Heredity
Zhi-xiang Lu
FRONTIERS IN GENETICS
(2014)
Article
Multidisciplinary Sciences
Jason Qian, Zhi-xiang Lu, Christopher P. Mancuso, Han-Ying Jhuang, Rocio del Carmen Barajas-Ornelas, Sarah A. Boswell, Fernando H. Ramirez-Guadiana, Victoria Jones, Akhila Sonti, Kole Sedlack, Lior Artzi, Giyoung Jung, Mohammad Arammash, Mary E. Pettit, Michael Melfi, Lorena Lyon, Sian V. Owen, Michael Baym, Ahmad S. Khalil, Pamela A. Silver, David Z. Rudner, Michael Springer
Article
Chemistry, Analytical
Christopher P. Mancuso, Zhi-Xiang Lu, Jason Qian, Sarah A. Boswell, Michael Springer
Summary: Quantitative diagnostics are crucial for controlling infectious diseases and informing treatment strategies. The qRPA technology provides semiquantitative information and may be useful for monitoring the progress of viral infections or other diseases.
ANALYTICAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Lan Lin, Peng Jiang, Juw Won Park, Jinkai Wang, Zhi-xiang Lu, Maggie P. Y. Lam, Peipei Ping, Yi Xing