Article
Multidisciplinary Sciences
Alex J. Vecchio, Sewwandi S. Rathnayake, Robert M. Stroud
Summary: The study reveals the molecular and structural basis of how CpE targets susceptible claudins, as well as differences in the primary CpE receptors in mice and humans due to sequence changes in the target motif.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biotechnology & Applied Microbiology
Kengo Ueda, Kazuki Kawahara, Narumi Kimoto, Yusuke Yamaguchi, Kazuhiro Yamada, Hiroya Oki, Takuya Yoshida, Shigeaki Matsuda, Yuki Matsumoto, Daisuke Motooka, Kentaro Kawatsu, Tetsuya Iida, Shota Nakamura, Tadayasu Ohkubo, Shinya Yonogi
Summary: This study characterized the genomic epidemiology of bec-harbouring C. perfringens strains and highlighted the importance of pCP13-like plasmids for the dissemination of pathogenic strains. The plasmids were found to have a high gene transfer rate and the ability to integrate various foreign genes, including those associated with virulence.
Article
Multidisciplinary Sciences
Daniel Alexander Castulo-Arcos, Roberto Adame-Gomez, Natividad Castro-Alarcon, Aketzalli Galan-Luciano, Maria Cristina Santiago Dionisio, Marco A. Leyva-Vazquez, Jose-Humberto Perez-Olais, Jeiry Toribio-Jimenez, Arturo Ramirez-Peralta
Summary: “This study investigated the presence of Bacillus cereus in coriander used for human consumption in southwestern Mexico and characterized its toxigenic profile, biofilm production, genetic diversity, and other properties. The results showed that 20% of the coriander samples were contaminated with B. cereus, and some strains carried genes associated with the production of diarrheal toxins. The B. cereus strains had a low capacity for biofilm formation and sporulation, and genetic analysis revealed clustering of strains isolated from different vegetable retailers in the same market.”
Article
Biotechnology & Applied Microbiology
Davide Porcellato, Siv Borghild Skeie, Hilde Mellegard, Marte Monshaugen, Stine Goransson Aanrud, Bjorn-Arne Lindstedt, Marina Aspholm
Summary: This study reveals the significant shift in composition and properties of Bacillus cereus group population present in milk during storage at different temperatures, providing valuable insights for the dairy industry to ensure high quality products and conduct risk assessments.
Article
Multidisciplinary Sciences
Akihiro Kawamoto, Tomohito Yamada, Toru Yoshida, Yusui Sato, Takayuki Kato, Hideaki Tsuge
Summary: Some bacteria have a toxin delivery system that transports enzymes into host cells. The bacterium Clostridioides difficile has a clinically important system with CDTa and CDTb proteins as therapeutic targets. The cryo-EM structure of CDTa bound to CDTb-pore was reported, revealing structural changes induced by CDTa binding and the involvement of an NSS-loop in substrate translocation. The dynamic information obtained provides insights for drug design against hypervirulent C. difficile strains.
NATURE COMMUNICATIONS
(2022)
Article
Chemistry, Multidisciplinary
Man Kyu Shim, Jinhee Na, In Kyung Cho, Eun Hyang Jang, Jooho Park, Sangmin Lee, Jong-Ho Kim
Summary: The study developed a claudin-4-targeting drug carrier that efficiently accumulated in pancreatic cancer tissues, suppressed tumor growth with minimal toxicity to normal tissues. This claudin-4-targeting drug carrier may represent a promising new approach for treating pancreatic cancer.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Food Science & Technology
Franziska Ramm, Marlitt Stech, Anne Zemella, Hendrik Frentzel, Stefan Kubick
Summary: The study analyzed the assembly of Hemolysin BL using a CHO cell-free system, demonstrating that both coexpression and individual synthesis of subunits are functional for active Hbl complex synthesis. Hbl showed the ability to perforate cell membranes, with a pre-pore-complex of L-1 and L-2 exhibiting cytotoxic effects, suggesting interaction with cell membranes. Overall, cell-free protein synthesis is an efficient method for studying multiple protein subunits assembly.
Article
Food Science & Technology
Kyung-Min Park, Hyun-Jung Kim, Kee-Jai Park, Minseon Koo
Summary: This study investigated the virulence potential of mesophilic and psychrotolerant Bacillus cereus isolated from chilled tofu, including prevalence, toxin gene profile, antibiogram, and biofilm formation. B. cereus carrying enterotoxin genes were more frequently found in psychrotolerant isolates. Hemolysin BL and nonhemolytic enterotoxin complexes were found at a higher frequency in psychrotolerant isolates. Some B. cereus isolates showed resistance to rifampicin or clindamycin. Mesophilic isolates displayed the strongest biofilm formation at 40 and 42 degrees C, while the biofilm formation of psychrotolerant isolates was not significantly affected by temperature.
Article
Food Science & Technology
Thea Neumann, Maren Krueger, Jasmin Weisemann, Stefan Mahrhold, Daniel Stern, Martin B. Dorner, Cecile Feraudet-Tarisse, Christopher Poehlmann, Katharina Schulz, Ute Messelhaeusser, Dagmar Rimek, Frank Gessler, Thomas Elssner, Stephanie Simon, Andreas Rummel, Brigitte G. Dorner
Summary: The study successfully identified nine specific mAbs targeting CPE and showed their high affinity in detecting CPE. Through ELISAs, CPE can be detected specifically, which is useful for diagnosing diarrhea in clinical laboratories.
Article
Immunology
Lea Schaefer, Frank Volk, Regina G. Kleespies, Johannes A. Jehle, Jorg T. Wennmann
Summary: Bacillus thuringiensis subsp. tenebrionis (Btt) produces a coleopteran-specific crystal protoxin protein (Cry3Aa delta-endotoxin). The strain NB125 was registered in 1990 for controlling the Colorado potato beetle, and gamma-irradiation resulted in the strain NB176-1 with higher cry3Aa production. Comparative genome analysis of NB125, NB176-1, and NB176 revealed differences in the cry3Aa gene and a chromosomal deletion in NB176.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Review
Entomology
Juan Luis Jurat-Fuentes, David G. Heckel, Juan Ferre
Summary: Insecticidal proteins from Bt bacterium are widely used as successful alternatives to synthetic pesticides, but the evolution of resistance in target pests remains a major threat. High-level resistance to Bt sprays is limited in the field, but several lepidopteran and coleopteran species have developed practical resistance to Bt proteins produced in transgenic plants.
ANNUAL REVIEW OF ENTOMOLOGY, VOL 66, 2021
(2021)
Article
Cell Biology
Vani Pande, Nivedita Mitra, Saket Rahul Bagde, Ramanujam Srinivasan, Pananghat Gayathri
Summary: This study provides insights into the effect of nucleotide state on membrane binding of Spiro plasma citri MreB5 (ScMreB5) and the allosteric effect of Glu134 mutation and the bound nucleotide on membrane binding. The study suggests that MreB protein modulates bacterial morphology by controlling polymerization and disassembly.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Fisheries
Melody Abena Anokyewaa, Kwaku Amoah, Yuan Li, Yishan Lu, Felix K. A. Kuebutornye, Berchie Asiedu, Iddrisu Seidu
Summary: This study assessed the safety of commercial Bacillus-based probiotics used in aquaculture in terms of virulence and drug resistance. Most isolates did not possess any of the virulence genes assessed, however, a few isolates carried enterotoxin genes. In terms of antibiotic resistance, most isolates were resistant to beta-lactam antibiotics.
AQUACULTURE REPORTS
(2021)
Review
Oncology
Amita R. Banga, Peace Odiase, Kartik Rachakonda, Amar P. Garg, Samuel E. Adunyah, Girish Rachakonda
Summary: This article discusses the potential value of the interaction between Clostridium perfringens Enterotoxin (CPE) and Claudin-4 in the treatment of breast cancer metastasis to the brain, and introduces the important role of Claudin-4 in cell trafficking and disease development.
Article
Microbiology
Michael Biggel, Danai Etter, Sabrina Corti, Peter Brodmann, Roger Stephan, Monika Ehling-Schulz, Sophia Johler
Summary: This study analyzed the whole-genome sequences of Bacillus thuringiensis isolates from biopesticides, food, and human fecal samples related to outbreaks. The isolates from food and outbreaks were found to be genomically matched with one of six biopesticide strains, indicating the biopesticide products as their source. The study also revealed a more diverse virulence gene profile in the biopesticide strains. Furthermore, high-quality genome assemblies of seven widely used B. thuringiensis biopesticide strains were provided, which will aid in microbial source tracking and risk assessment of B. thuringiensis-based biopesticides in the future.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Mechanics
Ramakrishna Manchi, R. Ponalagusamy
Summary: This theoretical study investigates the pulsatile flow of Sutterby nanofluid in an inclined porous tapered arterial stenosis, considering the simultaneous impact of electro-osmotic, magnetohydrodynamic, and periodic body forces. Gold nanoparticles of various shapes are used in the analysis, and the effects of different parameters on velocity, temperature, flow flux, impedance, skin-friction coefficient, and Nusselt number are analyzed through graphs and tables. The results show that the electro-osmotic parameter reduces hemodynamic factors significantly, while the shape of nanoparticles has a significant impact on heat flux at the stenosed arterial wall.
ARCHIVE OF APPLIED MECHANICS
(2021)
Editorial Material
Food Science & Technology
Daniel Ladant, Pascale Marchot, Sylvie Diochot, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Summary: This report presents the 27th annual meeting of the French Society of Toxinology, which focused on the dual nature of toxins and showcased the latest research findings on plant, fungal, algal, animal, and bacterial toxins.
Article
Mechanics
R. Ponalagusamy, D. Murugan
Summary: This paper investigates the dispersion problem of a solute in electro-magneto-hydrodynamic peristaltic flow of Jeffrey fluid in a uniform channel filled with porous medium and compliant walls. Through numerical computation and analysis of various physical parameters, valuable conclusions have been drawn. The findings of this research are significant for the field of artificial bio-processing.
KOREA-AUSTRALIA RHEOLOGY JOURNAL
(2022)
Review
Food Science & Technology
Michel R. Popoff, Holger Bruggemann
Summary: Clostridium botulinum and Clostridium tetani are bacteria that produce potent neurotoxins and cause paralysis. The synthesis of neurotoxins is regulated by complex networks involving environmental factors, nutrition, and regulatory pathways.
Review
Microbiology
Christine Rasetti-Escargueil, Michel R. Popoff
Summary: Botulinum neurotoxins are protein complexes produced by bacteria of the genus Clostridium. There is a need for fast, accurate, and reliable detection methods to monitor botulism outbreaks and for therapeutic and research purposes. Current methods include immunological and mass spectrometry approaches, but further validation is required. Cell-based assays have the potential to replace animal testing for potency determination and inhibitor identification, but their development still requires in vivo studies.
Article
Engineering, Mechanical
Ramasamy Ponalagusamy, Jaganmohan Sangeetha
Summary: In this article, the motion of two-phase immiscible fluids in a channel with a porous medium in the presence of a magnetic field is investigated. The impact of various physiological parameters on flow dynamics and electric potential nature is studied. The results show that the Casson parameter, Darcy numbers, and electric double layer (EDL) effects are the predominant features. These findings provide guidance for the pumping effect of gas pipelines or designing microfluidic devices.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART E-JOURNAL OF PROCESS MECHANICAL ENGINEERING
(2023)
Article
Food Science & Technology
Grazia Cottone, Letizia Chiodo, Luca Maragliano, Michel-Robert Popoff, Christine Rasetti-Escargueil, Emmanuel Lemichez, Therese E. Malliavin
Summary: Combined homology modeling and atomistic simulations are used to investigate the internal dynamics of botulinum neurotoxins (BoNTs). The study reveals that the conformational variations of BoNTs are influenced by pH and protein structure, and the mobility of the belt alpha-helix may interact with the core translocation domain during the translocation process, providing important insights into the molecular mechanism of BoNTs.
Article
Physics, Multidisciplinary
R. Ponalagusamy, D. Murugan
Summary: The present study analyzes the solute dispersion mechanism in a circular conduit with pulsatile flow of Carreau fluid. By using dispersion technique and finite Hankel transforms, the axial solute distribution process is analyzed. The effects of various physical parameters on the dispersion environment are investigated, and an increment in the Debye-Huckel parameter is found to enhance the dispersion mechanism. Furthermore, the study highlights the clinical aspect of solute dispersion in blood and its potential applications in medical and bioengineering fields.
CHINESE JOURNAL OF PHYSICS
(2023)
Review
Food Science & Technology
Michel R. R. Popoff, Sandra Legout
Summary: Louis Pasteur, a pioneer in microbiology, discovered anaerobic life and identified the first pathogenic anaerobic bacterium. He focused on the prevention and treatment of infectious diseases using attenuated pathogens. His collaborators investigated the mechanisms of pathogenicity and found that bacterial toxins induce symptoms and lesions in experimental animals.
Article
Materials Science, Biomaterials
Ria D. Corder, Robert B. Vachieri, Megan E. Martin, Darlene K. Taylor, Jodie M. Fleming, Saad A. Khan
Summary: It has been found that reducing the rigidity of the extracellular matrix (ECM) can slow down breast cancer development. In this study, the effects of injecting liberase enzyme on the rheology of mammary tumors were examined. The results showed that injecting liberase enzyme can decrease tumor volume and tissue modulus without affecting tissue viscoelasticity. Multiple injections of liberase enzyme resulted in smaller but stiffer tumors. Large amplitude oscillatory shear (LAOS) experiments were also performed to analyze the nonlinear rheological behavior of the tumors.
BIOMATERIALS SCIENCE
(2023)
Article
Cell Biology
Daria Chirita, Pauline Bronnec, Flora Magnotti, Sarah Dalmon, Amandine Martin, Michel Popoff, Mathieu Gerfaud-Valentin, Pascal Seve, Alexandre Belot, Anne Contis, Agnes Duquesne, Gaetane Nocturne, Irene Lemelle, Sophie Georgin-Lavialle, Guilaine Boursier, Isabelle Touitou, Yvan Jamilloux, Thomas Henry
Summary: This study reveals the role of the B30.2 domain and the central helical scaffold (CHS) domain in regulating pyrin inflammasome activation in Familial Mediterranean Fever (FMF). Mutations in these domains can have different functional impacts on the pyrin inflammasome, contributing to the diversity of pyrin-associated autoinflammatory diseases.
CELL DEATH & DISEASE
(2023)
Editorial Material
Food Science & Technology
Daniel Ladant, Gilles Prevost, Michel R. Popoff, Evelyne Benoit
Review
Food Science & Technology
Michel R. R. Popoff, Grazyna Faure, Sandra Legout, Daniel Ladant
Summary: Humans have encountered poisonous animals since ancient times. Certain animals and plants produce toxic substances that can be deadly or have medicinal effects. The term venom now refers specifically to poisons delivered by biting. Scientists from the Institut Pasteur in Paris pioneered the development of antivenom therapy, paving the way for research on venom constituents and their biological activities. The study of snake venoms conducted by scientists at the Institut Pasteur has greatly contributed to the field.
Article
Engineering, Mechanical
R. Ponalagusamy
Summary: This study focuses on the flow of seminal fluid through the ductus efferentes and examines the role of electro-osmotic force and cilia motion. The researchers use the K-L fluid model to describe the constitutive equation of semen liquid and solve the main equations of ciliary flow. They find that the introduction of electric force in the ciliary flow system decreases flow impedance and increases plug core radius. The study also investigates the effects of physiological parameters on the flow rates of rhesus monkey and human semen. By introducing an electric field and using a rheology model, the researchers are able to reduce flow resistance and increase the flow velocity of seminal fluid, which is beneficial for fertilization.
PROCEEDINGS OF THE INSTITUTION OF MECHANICAL ENGINEERS PART E-JOURNAL OF PROCESS MECHANICAL ENGINEERING
(2023)
Article
Multidisciplinary Sciences
R. Ponalagusamy, Ramakrishna Manchi
Summary: This theoretical study examines the impact of a porous wall with Brinkman and Darcy layers on blood flow through a stenotic artery. The results highlight the significant effects of the artery's porous wall on physiological flow quantities, particularly at the stenotic throat.
SN APPLIED SCIENCES
(2021)
