期刊
TOXINS
卷 4, 期 9, 页码 695-717出版社
MDPI
DOI: 10.3390/toxins4090695
关键词
aflatoxin; Aspergillus; oxylipin; G protein-coupled receptor (GPCR); quorum sensing; sclerotia
资金
- NSF [IOS-0965649]
- USDA-CSREES Microbial Biology program [2007-35319-18183]
- NIH [1 R01 Al065728-0]
- Direct For Biological Sciences
- Division Of Integrative Organismal Systems [0965649] Funding Source: National Science Foundation
Oxylipins regulate Aspergillus development and mycotoxin production and are also involved in Aspergillus quorum sensing mechanisms. Despite extensive knowledge of how these oxylipins are synthesized and what processes they regulate, nothing is known about how these signals are detected and transmitted by the fungus. G protein-coupled receptors (GPCR) have been speculated to be involved as they are known oxylipin receptors in mammals, and many putative GPCRs have been identified in the Aspergilli. Here, we present evidence that oxylipins stimulate a burst in cAMP in A. nidulans, and that loss of an A. nidulans GPCR, gprD, prevents this cAMP accumulation. A. flavus undergoes an oxylipin-mediated developmental shift when grown at different densities, and this regulates spore, sclerotial and aflatoxin production. A. flavus encodes two putative GprD homologs, GprC and GprD, and we demonstrate here that they are required to transition to a high-density development state, as well as to respond to spent medium of a high-density culture. The finding of GPCRs that regulate production of survival structures (sclerotia), inoculum (spores) and aflatoxin holds promise for future development of anti-fungal therapeutics.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据