☆ 4.7 Article

Development and Characterization of a Monoclonal Antibody against Ochratoxin B and Its Application in ELISA

TOXINS (2010)

期刊

TOXINS

卷 2, 期 6, 页码 1582-1594

出版社

MDPI AG

DOI: 10.3390/toxins2061582

关键词

mycotoxin; ochratoxin B; monoclonal antibody; ELISA; western analysis

类别

Food Science & Technology Toxicology

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摘要

A monoclonal antibody specific to ochratoxin B (OTB) was employed for the development of an indirect competitive OTB-ELISA. The optimized OTB-ELISA resulted in a limit of detection (LOD) for OTB of 3 mu g/L (8 nM), a limit of quantification (LOQ) of 3.7 mu g/L (10 nM), and a 50% inhibitory concentration (IC50) of 150 nM. Due to very low cross-reactivity to OTA (2.7%) and structurally related molecules (0%), this OTB-ELISA was found to be suitable to detect OTB with excellent precision in different matrices, i.e., beer, coffee and wine. Therefore, this OTB-ELISA will allow screening of OTB in food and feed products.

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