Occurrence of Ochratoxin A in Southern Spanish Generous Wines under the Denomination of Origin Jerez-Xeres-Sherry and 'Manzanilla' Sanlucar de Barrameda

The mycotoxin ochratoxin A (OTA) has toxic effects in animals; the most relevant of them is nephrotoxicity. OTA has also been classified as a possible carcinogen for humans (group 2B) by the International Agency for Research on Cancer (IARC). Therefore, exposure to OTA through contaminated food can represent health impairment to humans. The maximum permitted level for this mycotoxin in wine is 2.0 mu g/L. The presence of OTA in Spanish wines produced using the traditional methods under the Denomination of Origin Jerez-Xeres-Sherry and manzanilla Sanlucar de Barrameda was evaluated by a High performance Liquid Chromatography method with fluorescence detection and immunoaffinity column purification. A recovery of 95.4% and a limit of detection and quantification of 0.009 mu g/L and 0.02 mu g/L respectively, were achieved. In manzanilla, fino, amontillado and oloroso wine, the mean OTA values were 0.042, 0.044, 0.144, and 0.319 mu g/L, respectively. These levels are not different from other data given in the reference literature on white wines, although fino and manzanilla wines have very low OTA levels.