Article
Oncology
A. Pham, C. Selenou, E. Giabicani, V. Fontaine, S. Marteau, F. Brioude, L. David, D. Mitanchez, M. L. Sobrier, I. Netchine
Summary: This study extensively analyzed the methylation levels of imprinted control regions (ICRs) in iPSCs and found abnormal hypermethylation at certain ICRs, resulting in loss of parental imprinting (LOI) and biallelic expression of imprinted genes. They also discovered that the epiPS (TM) culture medium and culturing iPSCs under hypoxic conditions prevented hypermethylation at ICRs while preserving the properties of iPSCs. This suggests that human iPSCs are a promising cellular model to study the physiopathology of imprinting disorders (IDs) and test therapeutic strategies.
CLINICAL EPIGENETICS
(2022)
Article
Multidisciplinary Sciences
Yu Jin Jang, Mijeong Kim, Bum-Kyu Lee, Jonghwan Kim
Summary: The authors developed a simple procedure to convert human primed pluripotent stem cells into trophoblast stem-like cells using bone morphogenetic protein 4, providing an important opportunity to study human placenta development and pathology.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Glyn Nigel Stacey, Jie Hao
Summary: In recent years, significant progress has been made in China in establishing hPSCs for the manufacture of cell therapies. The National Stem Cell Resource Center and its partner organizations have over 600 hPSC lines formally recorded, with 47 of them also registered for European Commission funded research projects. This national and international coordination aims to promote the translation of Chinese hPSC-based products into clinical use according to established standards.
CELL PROLIFERATION
(2022)
Article
Cell & Tissue Engineering
Zhuoran Yu, Sihan Zhou, Minli Liu, Lijun Chen, Xue Zhang, Xu Yang, Hongdi Cui, Chaoqian Jiang, Yanjiao Lv, Tingsheng Yan, Qingran Kong, Zhonghua Liu
Summary: This study successfully derived and maintained stable porcine pluripotent stem cells (pLCDMs) using the LCDM culture system. Transcriptome analysis revealed the unique molecular characteristics and similarities/differences with human and mouse EPSCs. Furthermore, two small molecules in the culture system were found to play a crucial role in promoting trophoblast lineage differentiation potential of pLCDMs.
Review
Biochemistry & Molecular Biology
Daehwan Kim, Sangho Roh
Summary: Stem cell research is crucial for studying human diseases, genetic preservation, and improving animals. Efforts have been made to establish true ESCs in various species, although capturing true bESCs in cattle remains a challenge. This review discusses the difficulties in establishing bESCs with different culture conditions and introduces alternative induced pluripotent stem cells and extended pluripotent stem cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Menno ter Huurne, Hendrik G. Stunnenberg
Summary: During early embryonic development, the rapid increase in cell number and the expression of genes that control developmental decisions are tightly regulated and interconnected processes. Transcription factors and signaling pathways play a crucial role in maintaining pluripotency and cell cycle progression, while canonical cell cycle regulators impact molecular networks controlling pluripotency and lineage specification.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Review
Cell & Tissue Engineering
Chunmeng Yao, Ruqiang Yao, Haining Luo, Ling Shuai
Summary: This review describes in vitro germline specification studies, focusing on the reconstitution of PGCs, meiosis, signaling pathways, and factors involved in these processes.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell Biology
Ying Zhang, Jun Wei, Jiani Cao, Kehua Zhang, Yaojin Peng, Hongkui Deng, Jiuhong Kang, Guangjin Pan, Yong Zhang, Boqiang Fu, Shijun Hu, Jie Na, Yan Liu, Lei Wang, Lingmin Liang, Huanxin Zhu, Yu Zhang, Zi-Bing Jin, Jie Hao, Aijin Ma, Tongbiao Zhao, Junying Yu
Summary: "Requirements for Human-Induced Pluripotent Stem Cells" is the first set of guidelines in China on human-induced pluripotent stem cells. It provides detailed technical requirements and aims to promote international standardization.
CELL PROLIFERATION
(2022)
Article
Engineering, Biomedical
Hong Cao, Qian Zhou, Chungeng Liu, Yecen Zhang, Minghui Xie, Weihua Qiao, Nianguo Dong
Summary: This study investigates the effect of substrate stiffness on the differentiation of human-induced pluripotent stem cells (h-iPSCs) into valve endothelial-like cells (h-iVECs). The results show that moderate substrate stiffness can enhance the differentiation efficiency of h-iVECs and is regulated by the WNT/Calcineurin signaling pathway. This study emphasizes the importance of using substrate stiffness to achieve a more specific and mature differentiation of h-iVECs.
ACTA BIOMATERIALIA
(2022)
Article
Biochemical Research Methods
Julia Vallverdu, Raquel A. Martinez Garcia de la Torre, Inge Mannaerts, Stefaan Verhulst, Ayla Smout, Mar Coll, Silvia Arino, Teresa Rubio-Tomas, Beatriz Aguilar-Bravo, Celia Martinez-Sanchez, Delia Blaya, Catherine M. Verfaillie, Leo A. van Grunsven, Pau Sancho-Bru
Summary: Human iPSCs are differentiated into HSCs with growth factors for in vitro modeling. The protocol yields iPSC-HSCs with phenotypic and functional characteristics of primary HSCs, suitable for high-throughput in vitro studies. Coculturing iPSC-HSCs with hepatocytes allows for the formation of 3D hepatic spheroids, enabling modeling and drug screening studies.
Review
Biochemistry & Molecular Biology
Ines Milagre, Carolina Pereira, Raquel A. Oliveira
Summary: Human pluripotent stem cells (PSCs), including embryonic and induced pluripotent stem cells, are widely used in biomedical research for understanding development, disease progression, and drug discovery. However, concerns have been raised regarding their safety in regenerative medicine, particularly due to the occurrence of aneuploid cells resulting from errors in chromosome segregation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Olga A. Krasnova, Vitaly V. Gursky, Alina S. Chabina, Karina A. Kulakova, Larisa L. Alekseenko, Alexandra V. Panova, Sergey L. Kiselev, Irina E. Neganova
Summary: This study analyzed the morphology of human pluripotent stem cells to identify the best clones for further clinical applications. By analyzing different cell lines and phenotypes, informative markers were identified to separate the best clones.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Tianyuan Shi, Martin Cheung
Summary: Using rodents for modeling neurological diseases has limitations due to differences in genetics, neural development, and physiology between humans and rodents. In the past decade, induced pluripotent stem cells and induced neural stem cells generated through reprogramming somatic cells have offered a powerful alternative for studying disease pathogenesis and testing regenerative medicines. Urine-derived stem cells are an ideal cell source for reprogramming due to their proliferation, multipotency, epithelial nature, and ease of reprogramming.
CELL AND BIOSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mina Ishioka, Yuma Nihashi, Yoichi Sunagawa, Koji Umezawa, Takeshi Shimosato, Hiroshi Kagami, Tatsuya Morimoto, Tomohide Takaya
Summary: This study found that inhibition of nucleolin by iSN04 can promote the differentiation of cardiac mesoderm into cardiomyocytes but interferes with the differentiation of early mesoderm into the cardiac lineage. This is the first report on generating cardiomyocytes from pluripotent stem cells using a DNA aptamer. iSN04 does not induce hypertrophic responses in primary-cultured cardiomyocytes, making it useful and safe for regenerative therapy of heart failure using stem cell-derived cardiomyocytes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
