Article
Cell & Tissue Engineering
Maiko Sezaki, Subinoy Biswas, Sayuri Nakata, Motohiko Oshima, Shuhei Koide, Nicole Pui Yu Ho, Nobukazu Okamoto, Takeshi Miyamoto, Atsushi Iwama, Hitoshi Takizawa
Summary: This study characterizes the heterogeneity of hMSCs and their ability to efficiently form humanized ossicles in good donors by correlating with the frequency and functionality of chondrocyte progenitors. CD271(+)CD51(+) stromal cell subset was found to possess enhanced hMSC activity, with PALLADIN-negative cells displaying the greatest CFU-F potential in subsets enriched for greater chondrogenic ability. The findings contribute to better understanding and isolation of ossicle-forming hMSCs for optimized engineering of human bone organs.
STEM CELLS AND DEVELOPMENT
(2021)
Article
Cell & Tissue Engineering
Julia Riedl, Michael Pickett-Leonard, Cindy Eide, Mark Andreas Kluth, Christoph Ganss, Natasha Y. Frank, Markus H. Frank, Christen L. Ebens, Jakub Tolar
Summary: Recessive dystrophic epidermolysis bullosa (RDEB) is a rare, incurable skin disease caused by biallelic mutations in type VII collagen (C7), and advancements in treatment have been made by utilizing the immunomodulatory potential of mesenchymal stem cells (MSCs). A unique subset of skin-derived MSCs expressing ABCB5 have superior skin homing ability and may induce wound repair through increased expression of HOXA3 gene. Further exploration of the immunomodulatory mechanisms among MSC populations may have broader implications in regenerative medicine beyond RDEB treatment.
Article
Biotechnology & Applied Microbiology
Erwin Pavel Lamparelli, Maria Camilla Ciardulli, Valentina Giudice, Pasqualina Scala, Rosa Vitolo, Tina Patricia Dale, Carmine Selleri, Nicholas Robert Forsyth, Nicola Maffulli, Giovanna Della Porta
Summary: This study investigated the chondrogenic potentials of 3D high-density cultures of bone marrow and Wharton's Jelly-derived mesenchymal stromal cells (MSCs) supplemented with hTGF-beta 1. The study found that high-density cultures promoted chondrogenesis and that Wharton's Jelly-derived MSCs showed potential for chondrogenic commitment. These findings have important implications for cartilage regeneration and tissue engineering.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Cell Biology
Yuhang Liu, Xudong Zhang, Xiaodong Chen, Bingjun Zhang, Liming Dai, Chenglong Wang, Yang Li, Xiaoling Zhang
Summary: MicroRNAs are crucial in the chondrogenesis of bone marrow mesenchymal stem cells (MSCs) and regulate joint regeneration in osteoarthritis. This study aimed to determine the role of miR146a in the chondrogenic differentiation of MSCs and its underlying mechanisms. The results showed that miR146a inhibits chondrogenesis in MSCs by directly targeting Lsm11.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell & Tissue Engineering
K. Futrega, E. Music, P. G. Robey, S. Gronthos, R. Crawford, S. Saifzadeh, T. J. Klein, M. R. Doran
Summary: The study characterized ovine BMSC and evaluated their ability to repair cartilage defects, concluding that micro-pellets manufactured from ovine articular chondrocytes were more effective than those from ovine BMSC. While ovine BMSC could form cartilage-like tissue in vitro, their effective application in cartilage repair will require successful mitigation of hypertrophy and tissue integration issues.
STEM CELL RESEARCH & THERAPY
(2021)
Review
Cell Biology
Qianmin Gao, Lipeng Wang, Sicheng Wang, Biaotong Huang, Yingying Jing, Jiacan Su
Summary: Bone marrow mesenchymal stromal cells are a group of heterogeneous cells consisting of multipotent stem cells, including osteochondral and adipocyte progenitors. Recent studies have identified new subgroups of BMSCs and clarified their roles in normal and pathological conditions. Furthermore, key regulators and mechanisms controlling the fate of BMSCs have been revealed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Health Care Sciences & Services
Suncica Kapor, Milica Vukotic, Tijana Suboticki, Dragoslava Dikic, Olivera Mitrovic Ajtic, Milica Radojkovic, Vladan P. Cokic, Juan F. Santibanez
Summary: The study found that HU inhibits the growth of BMMSC, causing senescence-like changes and affecting cellular differentiation and immunoregulatory functions.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Cell Biology
Wu Duan, Huajie Zou, Nan Zang, Dongxia Ma, Bo Yang, Lin Zhu
Summary: Bone marrow adipose tissue (MAT) can have both local and systemic effects on metabolic homeostasis. The effects of metformin, a first-line drug for type 2 diabetes, on MAT and bone marrow mesenchymal stem cell (BM-MSC) differentiation are conflicting. In vivo and in vitro experiments showed that metformin increased MAT in mice tibia and promoted both osteogenic and lipogenic gene expression in mouse bone marrow. However, metformin inhibited lipogenesis and promoted osteogenesis of MSC in vitro. The increased MAT may be attributed to the filling of adipose tissue after metformin-induced apoptosis of bone marrow stromal cells.
Article
Engineering, Biomedical
Fangang Meng, Zibo Yang, Dianbo Long, Minghui Gu, Mei Shang, Anyu Zeng, Xingzhao Wen, Yueran Xue, Xiaoyi Zhao, Aishan He
Summary: The molecular weight of hyaluronan affects the chondrogenesis of bone marrow mesenchymal stem cells (BMSCs) seeded on TCP-COL-HA scaffolds, with higher molecular weight showing better chondrogenic induction.
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
(2022)
Article
Cell Biology
Sarah Hochmann, Kristy Ou, Rodolphe Poupardin, Michaela Mittermeir, Martin Textor, Salaheddine Ali, Martin Wolf, Agnes Ellinghaus, Dorit Jacobi, Juri A. J. Elmiger, Samantha Donsante, Mara Riminucci, Richard Schaefer, Uwe Kornak, Oliver Klein, Katharina Schallmoser, Katharina Schmidt-Bleek, Georg N. Duda, Julia K. Polansky, Sven Geissler, Dirk Strunk
Summary: Multipotent stromal cells are attractive sources for cell therapy and tissue engineering, but their application is limited due to functional diversity. In this study, we compared the regenerative potential of different stromal cell types in vitro and in vivo and found that only bone marrow stromal cells (BMSCs) could fully regenerate critical-size femoral defects. This ability was attributed to cell type-specific epigenetic landscapes controlling transcriptional stromal differentiation networks. BMSCs initiated defect healing and eventually contributed to tissue remodeling.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Review
Immunology
Kilian Kelly, John E. J. Rasko
Summary: GvHD is a life-threatening complication of hematopoietic stem cell transplantation, often requiring high-dose corticosteroid treatment. MSCs show promise as a potential therapy due to their immunosuppressive and immunoregulatory effects, but their efficacy may be influenced by donor variability and manufacturing processes.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell & Tissue Engineering
Rose Ann G. Franco, Eamonn McKenna, Pamela G. Robey, Ross W. Crawford, Michael R. Doran, Kathryn Futrega
Summary: For the use of bone marrow stromal cells (BMSC) in cartilage repair, controlling their hypertrophic differentiation is crucial. TGF-beta 1 stimulation triggers chondrogenic and hypertrophic differentiation in BMSCs, and silencing SP7, a transcription factor associated with hypertrophic differentiation, reduces both hypertrophic and chondrogenic differentiation processes. However, SP7 silencing compromises overall trilineage differentiation of BMSCs and makes it challenging to promote chondrogenesis while reducing hypertrophy in cartilage tissue engineering.
JOURNAL OF TISSUE ENGINEERING
(2023)
Review
Clinical Neurology
Zili Wang, Xudong Wang, Yidong Liao, Guangtang Chen, Kaya Xu
Summary: Stroke is a major cause of death and disability worldwide, and current treatment options are limited. Bone marrow mesenchymal stromal cells (BMSCs) offer a new approach for stroke treatment, but research on the precise inflammatory mechanism is lacking. In this review, we summarize the immune response of BMSCs in stroke treatment and provide a new perspective for stem cell therapy.
FRONTIERS IN NEUROLOGY
(2022)
Article
Engineering, Biomedical
Yue Yuan, Congxiao Wang, Shreyas Kuddannaya, Jia Zhang, Dian R. Arifin, Zheng Han, Piotr Walczak, Guanshu Liu, Jeff W. M. Bulte
Summary: This study demonstrates that high-mannose N-linked glycans can serve as a biomarker for label-free tracking of transplanted human mesenchymal stromal cells (hMSCs) using mannose-weighted chemical exchange saturation transfer (CEST) magnetic resonance imaging (MRI). This label-free imaging method may facilitate the development and testing of cell therapies.
NATURE BIOMEDICAL ENGINEERING
(2022)
Article
Ophthalmology
Ilham Putra, Xiang Shen, Khandaker N. Anwar, Behnam Rabiee, Ravand Samaeekia, Enmar Almazyad, Pushpanjali Giri, Sayena Jabbehdari, Mohammed R. Hayat, Abdelrahman M. Elhusseiny, Mahmood Ghassemi, Nadim Mahmud, Deepak P. Edward, Charlotte E. Joslin, Mark I. Rosenblatt, Reza Dana, Medi Eslani, Peiman Hematti, Ali R. Djalilian
Summary: The subconjunctival administration of MSCs was found to be safe and effective in promoting corneal epithelial wound healing in animal models, providing preclinical data to support a phase I clinical study. This therapy shows potential for future clinical applications in enhancing tissue repair.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2021)
Article
Orthopedics
Jasmijn Korpershoek, Lucienne A. Vonk, Tommy S. De Windt, Jon Admiraal, Esmee C. Kester, Nienke Van Egmond, Daniel B. F. Saris, Roel J. H. Custers
Article
Biotechnology & Applied Microbiology
Margot Rikkers, Riccardo Levato, Jos Malda, Lucienne A. Vonk
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2020)
Article
Orthopedics
Margot Rikkers, Koen Dijkstra, Bastiaan F. Terhaard, Jon Admiraal, Riccardo Levato, Jos Malda, Lucienne A. Vonk
Summary: PRP can alter migration and inflammation response of chondrocytes, stimulate proliferation, but strongly downregulate the production of cartilage ECM.
Letter
Orthopedics
Margot Rikkers, Koen Dijkstra, Bastiaan F. Terhaard, Jon Admiraal, Riccardo Levato, Jos Malda, Lucienne A. Vonk
Article
Orthopedics
Tim F. F. Saris, Tommy S. de Windt, Esmee C. Kester, Lucienne A. Vonk, Roel J. H. Custers, Daniel B. F. Saris
Summary: The study evaluated the 5-year follow-up of using allogenic MSCs as signaling cells in repairing symptomatic cartilage defects in the knee, showing significant improvement in patient outcomes with no serious adverse events, supporting the safety and efficacy of this novel approach in cartilage repair.
AMERICAN JOURNAL OF SPORTS MEDICINE
(2021)
Article
Cell Biology
Joao Pedro Garcia, Lizette Utomo, Imke Rudnik-Jansen, Jie Du, Nicolaas P. A. Zuithoff, Anita Krouwels, Gerjo J. V. M. van Osch, Laura B. Creemers
Summary: The study showed that OSM may play a significant role in inflammatory phenotypes of OA, with its expression correlating with synovitis and osteophyte formation, while its impact on cartilage damage varies depending on the model.
Article
Pharmacology & Pharmacy
Anna Tellegen, Martijn Beukers, Imke Rudnik-Jansen, Nicolien van Klaveren, Kan Loi How, Nina Woike, George Mihov, Jens Thies, Erik Teske, Laura Creemers, Marianna Tryfonidou, Bjorn Meij
Summary: This prospective study demonstrated the safety and proof-of-concept of IA-administered TA-PEAMs in treating OA pain and inflammation in dogs, showing potential for tissue regeneration.
Article
Cell & Tissue Engineering
Michella H. Hagmeijer, Jasmijn V. Korpershoek, Joao F. Crispim, Li-Ting Chen, Pascal Jonkheijm, Aaron J. Krych, Daniel B. F. Saris, Lucienne A. Vonk
Summary: Meniscus regeneration can be enhanced by targeting meniscus cells and MSCs with the right growth factors, combined with CMI (R). PDGF, TGF-beta 1, and PL were found to stimulate migration, proliferation, and extracellular matrix production in meniscus cells and MSCs. Functionalizing the CMI (R) with growth factor binding peptides holds great potential for meniscus replacement after partial meniscectomy.
JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Lucienne Angela Vonk, Giulietta Roel, Jacques Hernigou, Christian Kaps, Philippe Hernigou
Summary: Autologous chondrocyte implantation (ACI) is a cell therapy for focal cartilage defects, approved in the EU with spheroids of autologous matrix-associated chondrocytes. Preclinical and nonclinical studies evaluate the quality, safety, and efficacy of ACI, while clinical data show short- to mid-term safety and efficacy for the treatment of cartilage defects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Tijn Wiersema, Anna R. Tellegen, Martijn Beukers, Marijn van Stralen, Erik Wouters, Mandy van de Vooren, Nina Woike, George Mihov, Jens C. Thies, Laura B. Creemers, Marianna A. Tryfonidou, Bjorn P. Meij
Summary: The study found that intradiscal injection of CXB-PEAMs can effectively reduce pain interference with the daily activities of dogs suffering from back pain, with more pronounced effects observed in male dogs and those with no Modic changes in MRI. This treatment method may have the potential to not only alleviate pain but also halt the degenerative process long term or restore the disc.
Article
Chemistry, Multidisciplinary
Luana Zerrillo, Maria Rosa Gigliobianco, Domenico D'Atri, Joao Pedro Garcia, Fabio Baldazzi, Yanto Ridwan, Gaston Fuentes, Alan Chan, Laura B. Creemers, Roberta Censi, Piera Di Martino, Luis J. Cruz
Summary: This study developed new poly(lactide-co-glycolide) (PLGA) nanoparticles (NPs) surfaces decorated with hyaluronic acid (HA) to enhance targeted drug specificity to the osteoarthritic knee joint. The PLGA NPs grafted to sodium hyaluronate showed increased binding to cartilage cells and tissue, and enhanced accumulation at the target site. This study presents a safe drug-delivery system with improved receptor specificity, providing an advantageous alternative to current nanotherapies.
Article
Orthopedics
Lisanne T. Laagland, Frances C. Bach, Laura B. Creemers, Christine L. Le Maitre, Deepani W. Poramba-Liyanage, Marianna A. Tryfonidou
Summary: Increasing medium osmolarity during expansion improves the phenotype of NPCs, enhancing their potential as cell-based therapies for intervertebral disc regeneration.
Review
Pharmacology & Pharmacy
Adriano P. Pontes, Tim J. M. Welting, Jaap Rip, Laura B. Creemers
Summary: This review examines the use of polymeric nanoparticles (PNPs) as drug carriers for the treatment of osteoarthritis (OA). PNPs have shown promise due to their biodegradability, nanoscale structure, functional versatility, and reproducible manufacturing. The article discusses the current development of natural and synthetic PNPs for drug delivery and OA treatment, providing evidence from in vitro and pre-clinical in vivo studies on how these nanoparticle-based therapies modulate disease pathology and key cellular pathways. Additionally, the biodegradability and surface modification of the nanocarriers are compared in relation to drug release profile and tissue targeting, and the challenges of delivering nanoparticles to the cartilage are discussed.
Article
Orthopedics
Shaghayegh Basatvat, Frances C. Bach, Marcos N. Barcellona, Abbie L. Binch, Conor T. Buckley, Brian Bueno, Nadeen O. Chahine, Ana Chee, Laura B. Creemers, Stefan Dudli, Bailey Fearing, Stephen J. Ferguson, Jennifer Gansau, Benjamin Gantenbein, Rahul Gawri, Juliane D. Glaeser, Sibylle Grad, Julien Guerrero, Lisbet Haglund, Paula A. Hernandez, Judith A. Hoyland, Charles Huang, James C. Iatridis, Svenja Illien-Junger, Liufang Jing, Petra Kraus, Lisanne T. Laagland, Gernot Lang, Victor Leung, Zhen Li, Thomas Lufkin, Josette C. van Maanen, Emily E. McDonnell, Chris J. Panebianco, Steven M. Presciutti, Sanjna Rao, Stephen M. Richardson, Sarah Romereim, Tara C. Schmitz, Jordy Schol, Lori Setton, Dmitriy Sheyn, Joseph W. Snuggs, Y. Sun, Xiaohong Tan, Marianna A. Tryfonidou, Nam Vo, Dong Wang, Brandon Williams, Rebecca Williams, S. Tim Yoon, Christine L. Le Maitre
Summary: This study aims to standardize the extraction and expansion techniques for nucleus pulposus (NP) cells, in order to reduce variability between laboratories and improve comparability of results. The most commonly used methods for NP cell extraction, expansion, and re-differentiation were identified through a global survey. Recommended protocols for NP cell culture in different species were provided to support harmonization and cross-lab comparisons.
Article
Engineering, Biomedical
Shima Tavakoli, Austin Evans, Oommen P. Oommen, Laura Creemers, Jharna Barman Nandi, Jons Hilborn, Oommen P. Varghese
Summary: Labeling and visualization of extracellular matrix (ECM) components secreted by cells and living tissues is important for biomedical researchers. The use of bioorthogonal chemistry approaches allows for the specific visualization and labeling of ECM constituents without interfering with other chemical or biological events. This review summarizes recent advancements in using biorthogonal chemistry for metabolic labeling and visualization of ECM proteins and glycosaminoglycans, discusses challenges and limitations, and suggests potential solutions for improvement.
MATERIALS TODAY BIO
(2023)