期刊
STEM CELL REPORTS
卷 5, 期 6, 页码 1081-1096出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2015.10.013
关键词
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资金
- Canadian Institutes of Health Research (CIHR) Regenerative Medicine and Nanomedicine Initiative
- JDRF
- Stem Cell Technologies
- Stem Cell Network (SCN)
- Janssen RD, LLC
Pluripotent human embryonic stem cells (hESCs) are a potential source of transplantable cells for treating patients with diabetes. To investigate the impact of the host recipient on hESC-derived pancreatic progenitor cell maturation, cells were transplanted into immunodeficient SCID-beige mice or nude rats. Following the transplant, basal human C-peptide levels were consistently higher in mice compared with rats, but only rats showed robust meal- and glucose-responsive human C-peptide secretion by 19-21 weeks. Grafts from rats contained a higher proportion of insulin:glucagon immunoreactivity, fewer exocrine cells, and improved expression of mature f3 cell markers compared with mice. Moreover, ECM-related genes were enriched, the collagen network was denser, and blood vessels were more intricately integrated into the engrafted endocrine tissue in rats relative to mice. Overall, hESC-derived pancreatic progenitor cells matured faster in nude rats compared with SCID-beige mice, indicating that the host recipient can greatly influence the fate of immature pancreatic progenitor cells post-transplantation.
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