Article
Chemistry, Medicinal
Chamandi S. Dampalla, Athri D. Rathnayake, Anushka C. Galasiti Kankanamalage, Yunjeong Kim, Krishani Dinali Perera, Harry Nhat Nguyen, Matthew J. Miller, Trent K. Madden, Hunter R. Picard, Hayden A. Thurman, Maithri M. Kashipathy, Lijun Liu, Kevin P. Battaile, Scott Lovell, Kyeong-Ok Chang, William C. Groutas
Summary: The 3C-like protease (3CLpro) of SARS-CoV-2 has been validated as an effective target for developing therapeutics. Highly potent inhibitors have been successfully designed based on structure-guided design, and their mechanism of action has been established using high-resolution cocrystal structures.
JOURNAL OF MEDICINAL CHEMISTRY
(2022)
Article
Cardiac & Cardiovascular Systems
Thomas S. Metkus, Lori J. Sokoll, Andreas S. Barth, Matthew J. Czarny, Allison G. Hays, Charles J. Lowenstein, Erin D. Michos, Eric P. Nolley, Wendy S. Post, Jon R. Resar, David R. Thiemann, Jeffrey C. Trost, Rani K. Hasan
Summary: The study found that over 50% of intubated patients with severe COVID-19 had myocardial injury, which was associated with a higher mortality rate. Myocardial injury in COVID-19 was closely related to baseline comorbidities, age, and multisystem organ dysfunction. Compared with ARDS patients without COVID-19, COVID-19 patients were older and had poorer cardiac function, but had a lower incidence of myocardial injury.
Article
Cell Biology
Dario Bongiovanni, Melissa Klug, Olga Lazareva, Simon Weidlich, Marina Biasi, Simona Ursu, Sarah Warth, Christian Buske, Marina Lukas, Christoph D. Spinner, Moritz von Scheidt, Gianluigi Condorelli, Jan Baumbach, Karl-Ludwig Laugwitz, Markus List, Isabell Bernlochner
Summary: This study found that platelets of COVID-19 patients showed increased expression of activation markers without stimulation, and further elevated expression of P-selectin when stimulated with TRAP. However, COVID-19 platelets exhibited reduced capacity to express LAMP-3 and P-selectin upon TRAP stimulation. These findings suggest a hyperactivated platelet phenotype during SARS-CoV-2 infection, potentially contributing to the observed hypercoagulopathy in COVID-19 patients.
CELL DEATH & DISEASE
(2021)
Article
Virology
Enric Monreal, Sainz S. de la Maza, Pedro Gullon, Elena Natera-Villalba, Juan Luis Chico-Garcia, Alvaro Beltran-Corbellini, Javier Martinez-Sanz, Nuria Garcia-Barragan, Javier Buisan, Rafael Toledano, Araceli Alonso-Canovas, Paula Perez-Torre, Maria C. Matute-Lozano, Jose Luis Lopez-Sendon, Guillermo Garcia-Ribas, Inigo Corral, Jesus Fortun, Beatriz Montero-Errasquin, Luis Manzano, Luis Maiz-Carro, Lucienne Costa-Frossard, Jaime Masjuan
Summary: This study found that immunosuppression may reduce the risk of moderate to severe ARDS in COVID-19 patients, especially among those with autoimmune diseases.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Pediatrics
Tina Hsu, Jacquelyn R. Sink, Veronica Alaniz, Lida Zheng, Anthony J. Mancini
Summary: The study described three cases of acute genital ulceration in female patients after receiving COVID-19 vaccination or natural infection, confirming the possible association between ulceration and viral infection.
JOURNAL OF PEDIATRICS
(2022)
Article
Chemistry, Medicinal
Chamandi S. Dampalla, Yunjeong Kim, Naemi Bickmeier, Athri D. Rathnayake, Harry Nhat Nguyen, Jian Zheng, Maithri M. Kashipathy, Matthew A. Baird, Kevin P. Battaile, Scott Lovell, Stanley Perlman, Kyeong-Ok Chang, William C. Groutas
Summary: Novel inhibitors have been synthesized that effectively inhibit coronavirus protease, showing potential for treating COVID-19 and providing a means to access new chemical space and optimize pharmacological activity.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Letter
Immunology
Nadine Muller, Mareike Kunze, Fabienne Steitz, Neil J. Saad, Barbara Muehlemann, Joern I. Beheim-Schwarzbach, Julia Schneider, Christian Drosten, Lukas Murajda, Sandra Kochs, Claudia Ruscher, Jan Walter, Nadine Zeitlmann, Victor M. Corman
Summary: An outbreak of coronavirus disease with 74 cases related to a nightclub in Germany in March 2020 highlighted the potential for superspreader events, with staff members being particularly affected (56% attack rate) and likely causing sustained viral transmission after an event at the club. This event supports the current club closures as a precautionary measure to prevent further spread of the virus.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Multidisciplinary Sciences
Tarek Bayyoud, Angelika Iftner, Thomas Iftner, Karl Ulrich Bartz-Schmidt, Focke Ziemssen, Hans Boesmueller, Falko Fend, Jens Martin Rohrbach, Marius Ueffing, Michael Schindler, Sebastian Thaler
Summary: This study examined the retinal tissue and vitreous of deceased COVID-19 patients for the presence of SARS-CoV-2 RNA and found no significant viral RNA levels. Histopathological examinations showed no morphological signs of inflammation or vessel occlusions in the retina and vitreous. Further research is needed to confirm or refute these findings.
Article
Immunology
Henry M. Staines, Daniela E. Kirwan, David J. Clark, Emily R. Adams, Yolanda Augustin, Rachel L. Byrne, Michael Cocozza, Ana Cubas-Atienzar, Luis E. Cuevas, Martina Cusinato, Benedict Mo Davies, Mark Davis, Paul Davis, Annelyse Duvoix, Nicholas M. Eckersley, Daniel Forton, Alice J. Fraser, Gala Garrod, Linda Hadcocks, Qinxue Hu, Michael Johnson, Grant A. Kay, Kesja Klekotko, Zawditu Lewis, Derek C. Macallan, Josephine Mensah-Kane, Stefanie Menzies, Irene Monahan, Catherine M. Moore, Gerhard Nebe-von-Caron, Sophie Owen, Chris Sainter, Amadou A. Sall, James Schouten, Christopher T. Williams, John Wilkins, Kevin Woolston, Joseph Ra Fitchett, Sanjeev Krishna, Tim Planche
Summary: The study showed that in SARS-CoV-2 infection, 2.0%-8.5% of individuals did not seroconvert 3-6 weeks after infection. Those who did seroconvert were typically older, more likely to have concurrent conditions, and had higher levels of inflammatory markers. Non-White individuals had higher antibody concentrations compared to White individuals, and these concentrations remained stable during follow-up.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Critical Care Medicine
Yaseen M. Arabi, Dunia Jawdat, Ali H. Hajeer, Musharaf Sadat, Jesna Jose, Ramesh K. Vishwakarma, Walid Almashaqbeh, Abdulaziz Al-Dawood
Summary: This study evaluated the inflammatory response in patients with severe acute respiratory infection due to the Middle East respiratory syndrome and non-Middle East respiratory syndrome, and identified two distinct inflammatory subtypes using latent class analysis. The findings showed that a subset of patients exhibited increased levels of interleukins, indicative of a cytokine storm. Further research is needed to explore the effects of immunomodulators on different inflammatory subtypes.
CRITICAL CARE MEDICINE
(2021)
Article
Medicine, General & Internal
Weiwei Zhang, Min Zhang, Zhiming Kuang, Zhenfei Huang, Lin Gao, Jianlong Zhu
Summary: The study found that the incidence of acute respiratory distress syndrome (ARDS) is relatively high in patients with severe acute pancreatitis, and factors such as respiratory rate, APACHE II score, Ranson score, polymorphonuclear, C-reactive protein, and albumin are independent risk factors for ARDS. Early targeted prevention and treatment for these risk factors are necessary.
Article
Immunology
Gail L. Sondermeyer Cooksey, Christina Morales, Lauren Linde, Samuel Schildhauer, Hugo Guevara, Elena Chan, Kathryn Gibb, Jessie Wong, Wen Lin, Brandon J. Bonin, Olivia Arizmendi, Tracy Lam-Hine, Ori Tzvieli, Ann McDowell, Kirstie M. Kampen, Denise L. Lopez, Josh Ennis, Linda S. Lewis, Eyal Oren, April Hatada, Blanca Molinar, Matt Frederick, George S. Han, Martha Sanchez, Michael A. Garcia, Alana McGrath, Nga Q. Le, Eric Boyd, Regina M. Bertolucci, Jeremy Corrigan, Stephanie Brodine, Michael Austin, William R. K. Roach, Robert M. Levin, Brian M. Tyson, Jake M. Pry, Kristin J. Cummings, Debra A. Wadford, Seema Jain
Summary: State and local health departments in California established a surveillance system to monitor the presence of SARS-CoV-2 and other respiratory pathogens. Results from May 10, 2020 to June 12, 2021 showed a SARS-CoV-2 positivity rate of 19.6% among tested specimens and the presence of other respiratory pathogens.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Critical Care Medicine
Michelle W. Rudolph, Martin C. J. Kneyber, Lisa A. Asaro, Ira M. Cheifetz, David Wypij, Martha A. Q. Curley
Summary: This study aims to evaluate the use of neuromuscular blocking agents in pediatric acute respiratory distress syndrome (PARDS). It was found that early NMBA use was associated with a longer duration of mechanical ventilation (MV), but not with mortality or long-term cognitive and functional impairment.
CRITICAL CARE MEDICINE
(2022)
Article
Chemistry, Medicinal
Brendan T. Freitas, Daniil A. Ahiadorme, Rahul S. Bagul, Ian A. Durie, Samir Ghosh, Jarvis Hill, Naomi E. Kramer, Jackelyn Murray, Brady M. O'Boyle, Emmanuel Onobun, Michael G. Pirrone, Justin D. Shepard, Suzanne Enos, Yagya P. Subedi, Kapil Upadhyaya, Ralph A. Tripp, Brian S. Cummings, David Crich, Scott D. Pegan
Summary: In the past 20 years, both severe acute respiratory syndrome coronavirus-1 and severe acute respiratory syndrome coronavirus-2 have caused zoonotic outbreaks in humans. The PLpro enzyme from a subgroup 2b bat coronavirus has been studied to identify structural features and substrate specificity. Based on this, 30 novel noncovalent inhibitors for subgroup 2b PLpro enzymes were designed, providing new directions for antiviral development against this group of coronaviruses.
ACS INFECTIOUS DISEASES
(2022)
Review
Cardiac & Cardiovascular Systems
Apurba Barman, Mithilesh K. Sinha, Jagannatha Sahoo, Debasish Jena, Vikas Patel
Summary: The study evaluated the efficacy and safety of respiratory rehabilitation in patients recovering from SARS. Results showed that respiratory rehabilitation significantly improved exercise capacity and pulmonary function test parameters in SARS patients, with no significant adverse events reported. However, the improvement in activities of daily living and quality of life outcomes were limited. Further clinical trials are needed to determine the optimal respiratory rehabilitation program for SARS patients.
Article
Chemistry, Analytical
Xiao Xu, Tian Li, Zhongxing Xu, Hejia Wei, Ruoyun Lin, Bin Xia, Feng Liu, Na Li
ANALYTICAL CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Hejia Wei, Lei Wang, Xiaobai Ren, Wenyu Yu, Jian Lin, Changwen Jin, Bin Xia
Article
Oncology
Pengfei Ding, Xin Zhang, Shujuan Jin, Bo Duan, Pengxiang Chu, Yufei Zhang, Zhi-Nan Chen, Bin Xia, Fei Song
Article
Biochemistry & Molecular Biology
Bingjie Ouyang, Lei Wang, Shuo Wan, Yang Luo, Lu Wang, Jian Lin, Bin Xia
JOURNAL OF BIOMOLECULAR NMR
(2013)
Letter
Multidisciplinary Sciences
Lei Chen, Feng-Jiao Xin, Jue Wang, Jicheng Hu, Yuan-Yuan Zhang, Shuo Wan, Lu-Sha Cao, Chang Lu, Peng Li, S. Frank Yan, Dietbert Neumann, Uwe Schlattner, Bin Xia, Zhi-Xin Wang, Jia-Wei Wu
Article
Biochemistry & Molecular Biology
Bo Duan, Pengfei Ding, Timothy R. Hughes, William Wiley Navarre, Jun Liu, Bin Xia
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Yangjie Liao, Bo Duan, Yufei Zhang, Xinmin Zhang, Bin Xia
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Qian Huang, Bo Duan, Xianzhi Dong, Shilong Fan, Bin Xia
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biophysics
Qiran Zhai, Chen Lin, Bo Duan, Jun Liu, Lu Zhang, Bin Xia
Summary: WhiB4 protein, a member of the WhiB-like protein family, plays a crucial role in the survival and pathology of Mycobacterium tuberculosis. Acting as a transcription factor, WhiB4 regulates genes involved in redox balance, central metabolism, and respiration. It exists in different forms under different redox environments, including a dimeric holo form with iron-sulfur cluster, multimeric disulfide-linked oxidized apo forms, and a monomeric reduced apo form.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Chemistry, Multidisciplinary
Bo Duan, Dihong Fu, Chaoqun Zhang, Pengfei Ding, Xianzhi Dong, Bin Xia
Summary: The study reveals the molecular mechanisms of how TCF and GEF protein families selectively target unmethylated DNA sequences with a C-clamp type zinc finger domain. The structures of the C-clamp domains in HDBP1 and TCF1E were determined, showing a unique zinc finger fold and specific DNA recognition motifs. CpG base pairs play a central role in the binding mechanism, with multiple hydrogen bonds formed between specific residues in the C-clamp domain and the DNA backbone, highlighting the importance of unmethylated CpG binding proteins.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Biochemistry & Molecular Biology
Bo Duan, Pengfei Ding, William Wiley Navarre, Jun Liu, Bin Xia
Summary: Horizontal gene transfer (HGT) is a major driving force for bacterial evolution, with xenogeneic silencers playing a crucial role in recognizing and suppressing foreign genes to maintain genomic stability. The diversity in DNA recognition mechanisms of xenogeneic silencers leads to clear characteristics in DNA sequence preferences, correlated with different host genomic features. Xenogeneic silencers also act as a selective force against GC to AT mutational bias in bacterial genomes and help maintain host genomic AT contents at relatively low levels.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Biochemistry & Molecular Biology
Zhiliang Yuan, Zhi Qu, Bo Duan, Tianyi Wang, Jiajun Xu, Bin Xia
Summary: The C-terminal domain of M-pro-C can form a 3D domain-swapped dimer and amyloid fibrils under non-denaturing and 3D domain-swappable conditions, respectively. Positive correlations between domain swapping dimerization rates and amyloid fibrillation were found, but not essential, as mutants incapable of 3D domain swapping could still form fibrils. The unpacking of the protofibril core region during 3D domain swapping accelerates the amyloid fibrillation process.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Qian Huang, Bo Duan, Zhi Qu, Shilong Fan, Bin Xia
Summary: GapR, a nucleoid-associated protein found in Caulobacter crescentus, plays a crucial role in DNA replication, transcription, and cell division. It stimulates gyrase and topo IV to relax supercoils by binding to overtwisted DNA, facilitating the movement of replication and transcription machines. GapR forms a dimer-of-dimers structure in solution, with an open or closed conformation, and has intrinsic DNA binding preference towards AT-rich overtwisted DNA.
Article
Microbiology
Chen Lin, Yuting Tang, Yuchen Wang, Junli Zhang, Yeyu Li, Shuqin Xu, Bin Xia, Qiran Zhai, Yao Li, Lu Zhang, Jun Liu
Summary: WhiB4 plays a critical role in the disease progression and reactivation of Mycobacterium marinum infection. Targeting WhiB4 may be a promising strategy for the development of novel therapeutics to prevent the reactivation of latent tuberculosis infection.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biochemistry & Molecular Biology
Zhe Guan, Tiantian Cai, Zhongmin Liu, Yunfeng Dou, Xuesong Hu, Peng Zhang, Xin Sun, Hongwei Li, Yao Kuang, Qiran Zhai, Hao Ruan, Xuanxuan Li, Zeyang Li, Qihui Zhu, Jingeng Mai, Qining Wang, Luhua Lai, Jianguo Ji, Haiguang Liu, Bin Xia, Taijiao Jiang, Shu-Jin Luo, Hong-Wei Wang, Can Xie
