Article
Microbiology
Fernanda de Moura Alves, Jessica Correa Bezerra Bellei, Camila de Souza Barbosa, Caique Lopes Duarte, Amanda Luisa da Fonseca, Ana Claudia de Souza Pinto, Felipe Oliveira Raimundo, Barbara Albuquerque Carpinter, Ari Sergio de Oliveira Lemos, Elaine Soares Coimbra, Alex Gutterres Taranto, Vinicius Novaes Rocha, Fernando de Pilla Varotti, Gustavo Henrique Ribeiro Viana, Kezia K. G. Scopel
Summary: In this study, a virtual screening of chemical entities against Plasmodium falciparum molecular targets led to the discovery of a potential antimalarial compound. Compound 10 showed the best results against cerebral malaria, with significant parasite growth inhibition and improved survival in the experimental model. Furthermore, compound 10 reduced the synthesis of NO, indicating its potential in preventing cerebral malaria.
Article
Chemistry, Medicinal
Barbara Terroni, Luis Henrique Oliveira de Moraes, Aline Renata Pavan, Gerson Jhonatan Rodrigues, Jean Leandro Dos Santos
Summary: The study found that Lapdesf-4c can induce vasodilation and counteract endothelial dysfunction, showing great potential as a new drug candidate.
Review
Cell Biology
Mohammad Sheibani, Mehdi Ghasemi, Ahmad Reza Dehpour
Summary: Lithium has been a main therapy for bipolar disorders, but it can cause adverse effects such as sexual and erectile dysfunction. The mechanisms underlying these side effects involve both central and peripheral pathways. Understanding these mechanisms may lead to improved therapies for sexual dysfunction and better patient adherence.
Article
Chemistry, Medicinal
Zhongyuan Zheng, Hui Liu, Xi Wang, Yu Zhang, Shuiqing Qu, Yuanmin Yang, Shuoqiu Deng, Lina Chen, Xiaoxin Zhu, Yujie Li
Summary: This study confirmed the effects of AS+TMP on cerebral blood flow and NO-related indicators, and explored the mechanism of NO through the S-nitrosoproteome. The results demonstrated that AS+TMP could improve ECM symptoms.
ACS INFECTIOUS DISEASES
(2021)
Article
Medicine, Research & Experimental
Shupeng Hu, Qiangzhong Pi, Xiudan Xu, Jianghong Yan, Yongzheng Guo, Wanying Tan, An He, Zhe Cheng, Suxin Luo, Yong Xia
Summary: This study investigates the role of eNOS in vascular dysfunction induced by sepsis. Increased phosphorylation of eNOS in early sepsis leads to decreased vasodilation, while decreased levels of eNOS in late sepsis result in diminished nitric oxide-dependent relaxation responses.
Article
Immunology
Shupeng Hu, Qiangzhong Pi, Minghao Luo, Zhe Cheng, Xiaoxue Liang, Suxin Luo, Yong Xia
Summary: Endothelial dysfunction is a typical feature of sepsis, and the NLRP3/IL-1β axis may impair vasodilation by promoting eNOS proteolysis. Melatonin may protect against sepsis-induced endothelial relaxation dysfunction by inhibiting the NLRP3/IL-1β axis, suggesting its pharmacological potential in sepsis.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Amir Asgari, Paul Jurasz
Summary: Megakaryocytes are important members of the hematopoietic system, responsible for regulating vascular homeostasis through platelets. The process of megakaryopoiesis generates mature megakaryocytes which release proplatelets into blood vessels. Nitric oxide (NO) plays a modulatory role in hematopoiesis and hemostasis. This review summarizes the effect of NO and its signaling on megakaryopoiesis and thrombopoiesis under physiological and pathophysiological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jhana O. Hendrickx, Sofie De Moudt, Elke Calus, Peter Paul De Deyn, Debby Van Dam, Guido R. Y. De Meyer
Summary: This study investigates the association between arterial stiffness and cognitive decline by introducing arterial stiffness in genetic and pharmacological NO dysfunction models. The findings demonstrate that non-selective inhibition of NOS activity leads to cardiac dysfunction, arterial stiffness, and decline in hippocampal-dependent learning and memory, highlighting the importance of neuronal NOS in both cardiovascular and neurological pathophysiology.
Article
Oncology
Takahiro Anzai, Shinji Saijou, Yoshitsugu Ohnuki, Hiroshi Kurosawa, Masahiro Yasunaga, Yasuhiro Matsumura
Summary: Preliminary results suggest that TMEM180 may promote colon cancer growth, without significant effects on oxygen consumption or phosphorylated protein expression. However, glycolysis differs significantly between SW480 cells and TMEM180-knockdown cells. NGS analysis indicates that TMEM180 promotes enzyme expression in the nitric oxide synthesis system, potentially contributing to glucose and glutamine metabolism and cancer growth. Further basic studies of the TMEM180 molecule are warranted based on these findings.
TRANSLATIONAL ONCOLOGY
(2021)
Article
Medicine, Research & Experimental
Jun -Ha Hwang, Woong Heo, Jung Il Park, Kyung Min Kim, Ho Taek Oh, Gi Don Yoo, Jeekeon Park, Somin Shin, Youjin Do, Mi Gyeong Jeong, Eun Sook Hwang, Jeong-Ho Hong
Summary: Endothelial TAZ is involved in the production of nitric oxide (NO) and inhibits damage-induced liver fibrosis. It regulates NO production by transcriptional regulation of Nos3. Loss of TAZ leads to capillarization of liver sinusoidal endothelial cells and a decrease in NO concentration, accelerating liver fibrosis.
Article
Biochemistry & Molecular Biology
Giulia Querio, Susanna Antoniotti, Federica Geddo, Renzo Levi, Maria Pia Gallo
Summary: TMAO may be involved in impairing the endothelial-dependent vasodilatory mechanism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Kevin O'Gallagher, Francesca Puledda, Owen O'Daly, Matthew Ryan, Luke Dancy, Philip J. Chowienczyk, Fernando Zelaya, Peter J. Goadsby, Ajay M. Shah
Summary: This study reveals the fundamental physiological role of nNOS in regulating cerebral blood flow and functional connectivity in the human hippocampus.
CARDIOVASCULAR RESEARCH
(2022)
Article
Medicine, Research & Experimental
George J. Dugbartey
Summary: Kidney transplantation is the preferred treatment for patients with kidney failure. However, ischemia-reperfusion injury (IRI) poses a significant challenge to the long-term success of this intervention. Nitric oxide (NO) has emerged as a potential candidate for mitigating IRI and preventing graft rejection in kidney transplantation. This review provides an overview of the sources and protective effects of endogenous and exogenous NO in kidney transplantation.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Fabio Arias, Francisco Franco-Montalban, Miguel Romero, Juan Duarte, M. Dora Carrion, M. Encarnacion Camacho
Summary: This paper describes the design, synthesis, and biological evaluation of selective inhibitors of inducible nitric oxide synthase (iNOS). The most potent and selective compound showed inhibitory activity against iNOS without undesirable cardiovascular effects. In silico analysis predicted good drug-likeness properties for promising compounds, which also exhibited adequate cell viability. Docking studies revealed a unique binding mode for one compound and explained its selectivity towards iNOS.
BIOORGANIC CHEMISTRY
(2022)
Editorial Material
Plant Sciences
Francisco J. Corpas, Salvador Gonzalez-Gordo, Jose M. Palma
Summary: Nitric oxide is a crucial signaling molecule in plant physiological processes, but the enzymatic generation of nitric oxide in higher plants is still controversial.
TRENDS IN PLANT SCIENCE
(2022)
Meeting Abstract
Hematology
Kobina Dufu, Donna Oksenberg, Brian E. Cathers, Carlos J. Munoz, Cynthia R. Muller, Pedro Pedro Cabrales
Meeting Abstract
Hematology
Kobina Dufu, Donna Oksenberg, Brian E. Cathers, Carlos J. Munoz, Cynthia R. Muller, Pedro Pedro Cabrales
Article
Engineering, Biomedical
Martin T. Spang, Ryan Middleton, Miranda Diaz, Jervaughn Hunter, Joshua Mesfin, Alison Banka, Holly Sullivan, Raymond Wang, Tori S. Lazerson, Saumya Bhatia, James Corbitt, Gavin D'Elia, Gerardo Sandoval-Gomez, Rebecca Kandell, Maria A. Vratsanos, Karthikeyan Gnanasekaran, Takayuki Kato, Sachiyo Igata, Colin Luo, Kent G. Osborn, Nathan C. Gianneschi, Omolola Eniola-Adefeso, Pedro Cabrales, Ester J. Kwon, Francisco Contijoch, Ryan R. Reeves, Anthony N. DeMaria, Karen L. Christman
Summary: This study demonstrates the safety and feasibility of using intravascularly infused extracellular matrix as a biomaterial for tissue repair in animal models of acute myocardial infarction, traumatic brain injury, and pulmonary arterial hypertension. The biomaterial, consisting of decellularized myocardial tissue, localizes to injured tissues and is degraded in 3 days. In animal models of myocardial infarction, the intracoronary infusion of the biomaterial led to reduced left ventricular volumes, improved wall-motion scores, and differential gene expression associated with tissue repair and inflammation. This intravascular delivery of pro-healing extracellular matrix may provide translational advantages for healing inflamed tissues 'from the inside out'.
NATURE BIOMEDICAL ENGINEERING
(2023)
Article
Hematology
Kobina Dufu, Carsten Alt, Steven Strutt, James Partridge, Tzechiang Tang, Vincent Siu, Hilary Liao-Zou, Peter Rademacher, Alexander T. Williams, Cynthia R. Muller, Xin Geng, Mira Patel Pochron, Annie Nguyen Dang, Pedro Cabrales, Zhe Li, Donna Oksenberg, Brian E. Cathers
Summary: The pathophysiologic mechanism of sickle cell disease (SCD) involves polymerization of deoxygenated haemoglobin S (HbS), leading to red blood cell (RBC) sickling, decreased RBC deformability, microvascular obstruction, haemolysis, anaemia, and downstream clinical complications. Pharmacological increase in the concentration of oxygenated HbS in RBCs has been shown to be a novel approach to inhibit HbS polymerization and reduce RBC sickling and haemolysis. GBT021601, a small molecule that increases HbS-oxygen affinity, inhibits HbS polymerization and prevents RBC sickling in blood from patients with SCD. It also has positive effects on RBC health and haemoglobin levels in a murine model of SCD.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Review
Pharmacology & Pharmacy
Nanthini Jayabalan, Bryan Oronsky, Pedro Cabrales, Tony Reid, Scott Caroen, Aishwarya M. Johnson, Natalia A. Birch, John D. O'Sullivan, Richard Gordon
Summary: Chronic unresolving inflammation is a key feature of many diseases, and dysregulated immune and inflammasome activation contributes to its progression. The NLRP3 inflammasome, a large cytosolic protein complex, is well-studied and a potential target for therapeutic intervention. RRx-001, a direct NLRP3 inflammasome inhibitor, is currently being investigated in clinical trials and has shown promise in treating small cell lung cancer and other diseases driven by immune and inflammasome activation.
Review
Biochemistry & Molecular Biology
Robert J. J. Asaro, Elisabetta Profumo, Brigitta Buttari, Pedro Cabrales
Summary: The presence of red blood cells (RBCs) in the vasculature and their reactivity have sparked discussions about their role(s) in health and disease. These roles are linked to the development of adhesiveness and eventual clearance by macrophages in the spleen. This review explores the mechanisms involved and provides novel perspectives for identifying RBC adhesiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Vinay P. Jani, Alexander T. Williams, Vivek P. Jani, Amy G. Tsai, Marcos Intaglietta, Pedro Cabrales
Summary: This study presents a new method for analyzing left ventricular pressure-volume signals using the Prony algorithm, which fits and quantifies the transfer function. The analysis of pressure and volume signals during severe hemorrhagic shock and after resuscitation showed significant differences in the number of poles and the composite transfer function. The implementation of the Prony analysis in this study demonstrates its potential for future applications in various physiological and pathophysiological conditions.
MEDICAL ENGINEERING & PHYSICS
(2023)
Article
Immunology
Tony Reid, Bryan Oronsky, Scott Caroen, Mary Quinn, Jeannie Williams, Pedro Cabrales, Nacer Abrouk
Summary: This study aimed to evaluate the safety and feasibility of RRx-001 and nivolumab in patients with advanced cancer. The results showed that the combination of RRx-001 and nivolumab was safe and well-tolerated, with preliminary evidence of anti-cancer activity. Therefore, further clinical trials are warranted.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Yuri Chaves Martins, Arnon Dias Jurberg, Claudio Tadeu Daniel-Ribeiro
Summary: The concept of molecular mimicry describes the antigen sharing between parasites and hosts, which can benefit pathogen evasion from host immune responses, but it can also trigger autoimmunity. This concept has been extensively studied in humans and has raised increasing interest among immunologists. Through genomics and bioinformatics studies, it has been found that there is massive antigenic sharing between different organisms, but the degree of sharing is not related to pathogenicity or virulence. It is also rare for autoimmunity to develop in response to infections with microorganisms that have cross-reacting antigens, suggesting that molecular mimicry alone is not sufficient to disrupt self-tolerance mechanisms.
Article
Medicine, General & Internal
Sung Hwan Park, Kristen Hunter, Hugh Berry, Yuri Chaves Martins
Summary: This article describes a case of a young African American male who developed atrial fibrillation after starting gabapentin. The patient underwent successful cardioversion and was discharged on medication. The importance of monitoring for adverse effects of gabapentin, especially in young individuals, is emphasized.
JOURNAL OF MEDICAL CASE REPORTS
(2023)
Article
Parasitology
Vinay P. Jani, Alexander T. Williams, Leonardo Carvalho, Pedro Cabrales
Summary: This article investigates changes in oxygen delivery, flux, and utilization in animal models of cerebral malaria. The results show impaired convective and diffusive oxygen flux along with impaired pial hemodynamics in both susceptible and resistant models of cerebral malaria. Furthermore, late-stage cerebral malaria shows impaired oxygen transport and affinity, while early-stage cerebral malaria and resistant strains only show impaired oxygen transport.
EXPERIMENTAL PARASITOLOGY
(2023)
Article
Medicine, Research & Experimental
Derek R. Lamb, Alisyn Greenfield, Kiruphagaran Thangaraju, Saini Setua, Gena Eiker, Qihong Wang, Amid Vahedi, Mohd. Asim Khan, Ahmad Yahya, Pedro Cabrales, Andre F. Palmer, Paul W. Buehler
Summary: This study compares the impact of different sizes of polymerized hemoglobin on tissue oxygenation in hemorrhagic shock. The results suggest that intermediate-sized polymerized hemoglobin is more effective for resuscitation in hypercholesterolemic guinea pigs.
MOLECULAR PHARMACEUTICS
(2023)
Review
Parasitology
Yuri Chaves Martins, Flavia Lima Ribeiro-Gomes, Claudio Tadeu Daniel-Ribeiro
Summary: Innate immunity is the first line of defense against pathogens, cancer cells, and toxins. It also plays a crucial role in activating the body's specific immune response. This overview highlights the key findings and ideas that have contributed to the development of innate immunity as a branch of modern immunology.
MEMORIAS DO INSTITUTO OSWALDO CRUZ
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Pedro Cabrales, Tony Reid, Scott Caroen, Bryan Oronsky
Meeting Abstract
Cardiac & Cardiovascular Systems
Pedro Cabrales, Tony Reid, Scott Caroen, Bryan Oronsky
CIRCULATION RESEARCH
(2022)