Article
Immunology
Vivek Kumar Yadav, Pradeep Kumar Singh, Deepmala Sharma, Himanshu Pandey, Sunil Kumar Singh, Vishnu Agarwal
Summary: The AHL molecule 3-oxo-C12 HSL in Pseudomonas aeruginosa induces apoptotic features in human blood platelets, including PS exposure and mitochondrial dysfunction.
MICROBIAL PATHOGENESIS
(2021)
Article
Multidisciplinary Sciences
Parker Smith, Martin Schuster
Summary: Quorum sensing is a widespread cell density-dependent signaling mechanism in bacteria. This study demonstrates the role of antiactivators in preventing self-sensing in the quorum sensing system of Pseudomonas aeruginosa.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Raman Pachaiappan, Tharun Prasanna Rajamuthu, Ananya Sarkar, Pradiksha Natrajan, Nagasathiya Krishnan, Meenakumari Sakthivelu, Palaniyandi Velusamy, Palaniappan Ramasamy, Subash C. B. Gopinath
Summary: Flavonoids and isoflavonoids are effective inhibitors of biofilm formation and virulence factor production in Pseudomonas aeruginosa, with acacetin exhibiting the strongest inhibition capacity. These compounds downregulate the expression of virulence genes, making them potential targets for anti-quorum sensing strategies.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Sahana Vasudevan, Parthasarathy Srinivasan, Prasanna Neelakantan, John Bosco Balaguru Rayappan, Adline Princy Solomon
Summary: In this study, a novel photoluminescence-based assay using cysteamine-capped titanium oxide nanoparticles was developed for the rapid and sensitive diagnosis of microbial infections. The system showed a maximum relative response of 98% for AHLs produced by Pseudomonas aeruginosa, with a linear detection range of 10-160 nM in simulated host urine. The efficacy and specificity of the system in detecting AHLs produced by P. aeruginosa were confirmed in a temporal manner.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ankita Chopra, Dattatray Mongad, Surekha Satpute, Pranab Behari Mazumder, Praveen Rahi
Summary: The study aimed to isolate and characterize tea rhizobacteria that produce quorum sensing molecules and exhibit multiple plant growth-promoting activities. Thirty-one isolates were obtained from the tea rhizosphere, and two isolates (RTE1 and RTE4) were selected for their ability to inhibit tea fungal pathogens and produce AHL molecules. Genome-based analysis confirmed RTE1 as Burkholderia cepacia and RTE4 as Pseudomonas aeruginosa. Detailed characterization of the AHLs produced by the isolates revealed different chain lengths. Confocal microscopy showed biofilm formation by RTE1 and RTE4. Several genes involved in quorum sensing, plant growth promotion, and inducing systemic resistance were identified. Based on their functional traits, B. cepacia RTE1 and P. aeruginosa RTE4 are promising candidates for enhancing the health and growth of tea plantations.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Rachith Kalgudi, Roya Tamimi, Godfrey Kyazze, Tajalli Keshavarz
Summary: The study revealed that the combined use of farnesol and tyrosol had a better effect on Pseudomonas aeruginosa biofilm growth, especially against the RBHi strain, while the addition of quorum quenchers (QQs) reduced IL-8 secretion by A549 cells.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
I. F. Karimov, T. O. Fedorova, O. O. Zherebyateva, S. D. Borisov, E. A. Mikhailova
Summary: Acyl derivatives of homoserine lactone play a key role in intercellular communication of gram-negative bacteria, regulating collective behavior, virulence activation, resistance, and other properties. This study shows that temperature and time have significant effects on the production of signaling molecules in Pseudomonas strains.
Article
Medicine, Research & Experimental
V. S. Cherepushkina, T. E. Mironova, V. N. Afonyushkin, V. Yu Koptev, E. Nefedova, N. A. Donchenko, A. S. Dimova
Summary: The study investigated the impact of early accumulation of N-3-oxo-dodecanoyl-homoserine lactone on Pseudomonas aeruginosa reproduction, biofilm formation, and elastase activity. It was found that at a concentration of 25 μM, the lactone slowed down bacterial cell proliferation and promoted biofilm formation, while at a concentration of 0.75 μM, elastase activity was significantly increased.
BULLETIN OF EXPERIMENTAL BIOLOGY AND MEDICINE
(2021)
Article
Immunology
Melanie Gonzales, Laure Plener, Jean Armengaud, Nicholas Armstrong, Eric Chabriere, David Daude
Summary: This study demonstrates the importance of Quorum sensing in Burkholderia thailandensis using an enzyme-based quorum quenching strategy. The disruption of quorum sensing greatly affects bacterial behavior, including motility, proteolytic activity, and antimicrobial molecule production. Furthermore, the study shows that quorum sensing disruption significantly enhances the bactericidal activity against fungi and yeast. These findings are of prime interest for understanding the virulence of Burkholderia species and developing alternative treatments.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Plant Sciences
Siqi Liu, Xixian Zhu, Zhenchen Yan, Hui Liu, Lianhui Zhang, Wenjuan Chen, Shaohua Chen
Summary: Quorum sensing is a communication mechanism used by microorganisms to regulate population density and behavior. Quorum quenching, which interferes with this communication, shows promise in plant disease control. In this study, a quenching strain of Pseudomonas multiresinivorans QL-9a was isolated, and it demonstrated excellent degradation ability against signaling molecules and reduced the severity of bacterial soft rot caused by Pectobacterium carotovorum.
Article
Physics, Fluids & Plasmas
Brian P. Weaver, Christoph A. Haselwandter, James Q. Boedicker
Summary: Quorum sensing is a mechanism that allows bacterial cells to sense and regulate multicellular processes. Recent experiments have shown that extracellular vesicles can package and deliver signal molecules, but how this affects communication and coordination of multicellular behaviors is not well understood.
Article
Environmental Sciences
Bernadett Zara, Mate Polgar, Gyoergy Sipos, Gabor Doka, Parag Gogate, Vladimir Djokovic, Levente Csoka
Summary: Hydrodynamic cavitation treatment can induce the inactivation of bacterial signal molecules through shear effects and hydroxyl radicals, with the strongest effects achieved at speeds > 2000 rpm, consistent with literature data.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Engineering, Environmental
Ying Xu, Thomas Curtis, Jan Dolfing, Yonghong Wu, Bruce E. Rittmann
Summary: Research findings suggest that the regulation of N-acyl-homoserine-lactones (AHLs) via quorum sensing signaling can optimize phosphorus (P) entrapment in microbial aggregates, promoting microbial growth and enhancing the community of polyphosphate-accumulating organisms (PAOs) to increase both intra-and extra-cellular P capture.
Article
Microbiology
Zahid Ur Rehman, Afaque A. Momin, Abdullah Aldehaiman, Tayyaba Irum, Raik Grunberg, Stefan T. Arold
Summary: Quorum quenching (QQ) is the enzymatic degradation of molecules used by bacteria for synchronizing their behavior within communities. QQ has potential applications in water treatment, aquaculture, and healthcare due to its ability to inhibit biofilm formation and suppress the production of virulence factors. LrsL is a metallo-beta-lactamase superfamily QQ enzyme with exceptional catalytic features and high affinity for acyl-homoserine lactones (AHLs).
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Cell Biology
Shiyu Tao, Yi Xiong, Dandan Han, Yu Pi, Hanlu Zhang, Junjun Wang
Summary: Microbes use quorum sensing (QS) autoinducers for population communication, with N-(3-oxododecanoyl)- l-homoserine lactone (3OC12) from Pseudomonas aeruginosa LasI-LasR system having deleterious effects on intestinal epithelial cells. 3OC12 and 3OC14 trigger apoptosis rather than necrosis and ferroptosis in intestinal epithelial cells, reduce ECM-receptor interaction pathway gene expression, and decrease protein expressions from ECM and tight junction-associated pathway. Furthermore, 3OC12 and 3OC14 disrupt the intestinal epithelial barrier by inducing oxidative stress and apoptosis, with NAC showing potential for alleviating the syndrome caused by QS autoinducers exposure.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Maike Thamsen, Rajarshi Ghosh, Vincent C. Auyeung, Alexis Brumwell, Harold A. Chapman, Bradley J. Backes, Gayani Perara, Dustin J. Maly, Dean Sheppard, Feroz R. Papa
Article
Multidisciplinary Sciences
Aeid Igbaria, Philip I. Merksamer, Ala Trusina, Firehiwot Tilahun, Jeffrey R. Johnson, Onn Brandman, Nevan J. Krogan, Jonathan S. Weissman, Feroz R. Papa
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2019)
Article
Veterinary Sciences
B. Vita, G. A. Monteiro, C. M. Melo, R. R. Maziero, M. T. Carmo, M. A. Alvarenga, P. A. Dutra, Y. F. R. Sancler-Silva, F. O. Papa
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2019)
Article
Veterinary Sciences
G. A. Monteiro, C. Ramires Neto, Y. F. R. Sancler-Silva, L. Z. Oliveira, C. P. F. D. Aqua, F. P. Hartwig, P. M. Papa, J. Pantoja, D. F. Silva, F. O. Papa
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2019)
Review
Endocrinology & Metabolism
Rajarshi Ghosh, Kevin Colon-Negron, Feroz R. Papa
MOLECULAR METABOLISM
(2019)
Article
Oncology
Paul C. Moore, Jenny Y. Qi, Maike Thamsen, Rajarshi Ghosh, Justin Peng, Micah J. Gliedt, Rosa Meza-Acevedo, Rachel E. Warren, Annie Hiniker, Grace E. Kim, Dustin J. Maly, Bradley J. Backes, Feroz R. Papa, Scott A. Oakes
Article
Endocrinology & Metabolism
Tatsuya Ishibashi, Shuhei Morita, Shohei Kishimoto, Shinsuke Uraki, Ken Takeshima, Yasushi Furukawa, Hidefumi Inaba, Hiroyuki Ariyasu, Hiroshi Iwakura, Hiroto Furuta, Masahiro Nishi, Feroz R. Papa, Takashi Akamizu
JOURNAL OF DIABETES INVESTIGATION
(2020)
Article
Biochemistry & Molecular Biology
Yusuke Yamashita, Shuhei Morita, Hiroki Hosoi, Hiroshi Kobata, Shohei Kishimoto, Tatsuya Ishibashi, Hiroyuki Mishima, Akira Kinoshita, Bradley J. Backes, Koh-Ichiro Yoshiura, Feroz R. Papa, Takashi Sonoki, Shinobu Tamura
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Biochemistry & Molecular Biology
Hannah C. Feldman, Rajarshi Ghosh, Vincent C. Auyeung, James L. Mueller, Jae-Hong Kim, Zachary E. Potter, Venkata N. Vidadala, B. Gayani K. Perera, Alina Olivier, Bradley J. Backes, Julie Zikherman, Feroz R. Papa, Dustin J. Maly
Summary: The development of partial antagonists of IRE1 alpha RNase (PAIRs) allows for adaptive splicing of Xbp1 mRNA while suppressing destructive ER mRNA decay and apoptosis, capturing an intermediate RNase-inhibitory 'sweet spot' for drugging the UPR sensor/effector.
NATURE CHEMICAL BIOLOGY
(2021)
Article
Veterinary Sciences
S. N. Oliveira, E. A. B. de Araujo, L. F. M. C. Silva, R. M. Hayashi, V. R. Merenda, F. O. Papa
Summary: Fractionated semen collection can accurately identify the presence of various pathologies in stallion ejaculate, but findings may sometimes be vague, necessitating additional examinations for a definitive diagnosis.
EQUINE VETERINARY EDUCATION
(2022)
Article
Agriculture, Multidisciplinary
Lucas Emanuel Ferreira Canuto, Lorenzo Garrido Teixeira Martini Segabinazzi, Endrigo Adonis Braga de Araujo, Luis Fernando Merces Chaves Silva, Sidnei Nunes de Oliveira, Felipe Morales Dalanezi, Jose Antonio Dell'Aqua Junior, Frederico Ozanam Papa, Eunice Oba
Summary: This study evaluated the effects of two antioxidants on the parameters of cooled and frozen-thawed ram semen. The results showed that adding quercetin or BHT did not significantly affect sperm parameters. Further research is needed to explore the effects of these antioxidants on the fertility of cryopreserved ram semen.
SEMINA-CIENCIAS AGRARIAS
(2022)
Article
Agriculture, Multidisciplinary
Fernando Evaristo da Silva, Jaqueline Candido Carvalho, Camila de Paula Freitas Dell'Aqua, Frederico Ozanam Papa, Marc Roger Jean Marie Henry, Eunice Oba, Joao Carlos Pinheiro Ferreira
SEMINA-CIENCIAS AGRARIAS
(2020)