Article
Biotechnology & Applied Microbiology
Krystyna Dudaniec, Kerstin Westendorf, Elfriede Nossner, Wolfgang Uckert
Summary: EBV infections are usually cleared by immune cells, but in some cases can lead to cancer development. We isolated eight EBV-specific TCRs, which, after contact with antigens, can lyse tumor cells and serve as valuable targets for treating EBV-associated diseases.
HUMAN GENE THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Yingxue Gao, Yuxin Fu, Jia Wang, Xiang Zheng, Jianhua Zhou, Jian Ma
Summary: Epstein-Barr virus (EBV) is highly prevalent worldwide and is closely associated with gastric cancer, especially EBV associated Gastric cancer (EBVaGC). Understanding the mechanisms of EBV-induced gene hypermethylation in gastric cancer is crucial, and targeting EBV-induced DNA methylation may be a potential therapeutic approach for EBVaGC.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biophysics
Han-Yun Hsieh, Ray Chang, Yung-Yu Huang, Po-Han Juan, Hidetoshi Tahara, Kuan-Yi Lee, Di Ngoc Kha Vo, Ming-Han Tsai, Pei-Kuen Wei, Horn-Jiunn Sheen, Yu-Jui Fan
Summary: This study successfully detected the DNA sequence of LMP1 using a combination of microfluidic PCR and gold nanoslit-based SPR sensor, achieving rapid and accurate analytical procedures, as well as enhancing the detection efficiency of DNA extracted from EBV-positive cells.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Infectious Diseases
Anna E. Coghill, Jianwen Fang, Zhiwei Liu, Chien-Jen Chen, Ruth F. Jarrett, Henrik Hjalgrim, Carla Proietti, Kelly J. Yu, Wan-Lun Hsu, Pei-Jen Lou, Chen-Ping Wang, Yingdong Zhao, Denise L. Doolan, Allan Hildesheim
Summary: This study examined the IgG antibody responses against 115 EBV peptide sequences in 316 cancer-free adults and identified eight EBV peptide sequences that appear to play a role in immunogenicity. This finding is important for understanding the role of EBV in B-cell immunity in humans.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Microbiology
Snjezana Zidovec-Lepej, Margarita Batovic, Marija Rozman, Kristian Bodulic, Laura Prtoric, Ante Sokota, Andrea Nikcevic, Petra Simicic, Goran Tesovic
Summary: Through analysis of 73 patients with infectious mononucleosis (IM), it was found that EBV type 1 was predominant and there was a limited repertoire of LMP1 variants, with some patients having multiple LMP1 variants. Co-infections with different LMP1 variants were associated with increased levels of C-reactive protein and decreased neutrophil and platelet counts.
Article
Virology
Michael Hulse, Sarah M. Johnson, Sarah Boyle, Lisa Beatrice Caruso, Italo Tempera
Summary: The study demonstrates that ectopic expression of LMP1 and EBV-mediated B-cell growth transformation induces significant changes in fatty acids and lipid droplet formation. Inhibiting lipogenesis leads to preferential killing of LMP1-expressing B cells and hinders EBV immortalization of primary B cells. Targeting LMP-induced lipogenesis could be an effective strategy in treating LMP1-positive EBV-associated malignancies, distinguishing their treatment from EBV-negative counterparts.
JOURNAL OF VIROLOGY
(2021)
Article
Oncology
Wenzhe Li, Atsushi Okabe, Genki Usui, Masaki Fukuyo, Keisuke Matsusaka, Bahityar Rahmutulla, Yasunobu Mano, Takayuki Hoshii, Sayaka Funata, Nobuhiro Hiura, Masashi Fukayama, Patrick Tan, Tetsuo Ushiku, Atsushi Kaneda
Summary: The study showed that EBV infection contributes to gastric tumorigenesis by activating enhancers, with EHF playing a critical role in the process. Additionally, genes associated with the WNT signaling pathway in cancer were significantly enriched in EBV-positive GC.
Article
Oncology
Haiwen Chen, Xiaobing Duan, Xiaohong Deng, Yingping Huang, Xiang Zhou, Shanshan Zhang, Xiao Zhang, Pingjuan Liu, Chaopin Yang, Guojun Liu, Qinqin Ren, Yan Xiong, Bo Zhu, Jiexia Zhang, Tong Xiang
Summary: Nasopharyngeal carcinoma is an EBV-associated epithelial malignancy characterized by infiltration of lymphocytes, including NK cells. EBV-positive NPC cells often develop mechanisms to evade NK cell immune surveillance. Understanding the mechanisms involved in EBV-induced NK cell dysfunction may lead to the development of novel immunotherapies for NPC.
CANCER IMMUNOLOGY RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Saidu Kamara, Yanru Guo, He Wen, Ying Liu, Lei Liu, Maolin Zheng, Jing Zhang, Luqi Zhou, Jun Chen, Shanli Zhu, Lifang Zhang
Summary: Antibodies are important therapeutic agents in cancer treatment, with bispecific antibodies (BsAbs) being a next-generation strategy. However, their large size leads to poor tumor penetration. On the other hand, affibody molecules have shown promise in molecular imaging diagnostics and targeted tumor therapy.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Microbiology
Xintong Peng, Yanling Zhou, Yongguang Tao, Shuang Liu
Summary: This article summarizes the relationship between EBV, EGFR, and LMP1 in NPC, highlighting the importance of EGFR in the occurrence and development of NPC. EGFR-targeted drugs are discussed as potential newly developed anti-tumor drugs for NPC patients, as activation of EGFR signaling affects various aspects of NPC cell behavior and contributes to poor outcomes.
Review
Oncology
Angela Kwok-Fung Lo, Christopher W. Dawson, Hong Lok Lung, Ka-Leung Wong, Lawrence S. Young
Summary: LMP1, as a critical protein in EBV infection, plays an important role in the development of NPC by activating multiple pathways. Targeting LMP1 protein for treatments may be a novel therapeutic strategy for EBV-driven malignancies.
FRONTIERS IN ONCOLOGY
(2021)
Article
Microbiology
Katherine A. Willard, Ashley P. Barry, Cliff I. Oduor, John Michael Ong'echa, Jeffrey A. Bailey, Ann M. Moormann, Micah A. Luftig
Summary: In this study, we describe an unusual spontaneous lytic phenotype in EBV strains isolated from Kenyan endemic Burkitt lymphoma patients. These findings provide insight into viral and host factors involved in this process and the potential contribution of promiscuous lytic activity to the formation of EBV-associated diseases.
Review
Biochemistry & Molecular Biology
Nan Zhang, Yuxin Zuo, Liping Jiang, Yu Peng, Xu Huang, Lielian Zuo
Summary: This paper reviews the mechanisms underlying the role of EBV in the development of central nervous system diseases, discussing its effects on neurons and glial cells, as well as its promotion of proliferative disorders in B and T lymphocytes. However, the specific underlying molecular mechanisms remain unclear.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Pathology
Ayako Kume, Aya Shinozaki-Ushiku, Akiko Kunita, Atsushi Kondo, Tetsuo Ushiku
Summary: This study investigated the expression profiles of PD-L1 and EBV latent genes in EBV-associated malignant lymphomas and LPDs. Classic Hodgkin lymphoma showed the highest PD-L1 expression, followed by diffuse large B-cell lymphoma/Burkitt lymphoma, LPDs, and extranodal NK/T-cell lymphoma. LMP1 upregulates PD-L1 expression and may serve as a potential biomarker for predicting the efficacy of immune checkpoint inhibitors. The heterogeneous expression of PD-L1 and EBV latent genes may contribute to resistance to targeted therapies.
AMERICAN JOURNAL OF SURGICAL PATHOLOGY
(2022)
Article
Virology
Sara B. York, Stephanie N. Hurwitz, Xia Liu, David G. Meckes Jr
Summary: Epstein-Barr virus (EBV) is associated with various cancers and its oncogene LMP1 is secreted in small extracellular vesicles (EVs). The protein CD63 forms a complex with LMP1 and can be transported to EVs through ceramide. Therefore, we propose that ceramide is necessary for efficient packaging of LMP1 into small EVs.