Article
Microbiology
Sophie oehlmann, Ann-Kathrin Krieger, Nicolas Gisch, Marita Meurer, Nicole de Buhr, Maren von Koeckritz-Blickwede, Nicole Schuetze, Christoph Georg Baums
Summary: This study demonstrates that Streptococcus suis specifically modulates its interaction with host immune cells by modifying its cell surface.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Immunology
Minjing Wu, Shan Huang, Jingyun Du, Yijun Li, Shan Jiang, Ling Zhan, Xiaojing Huang
Summary: This study investigates the impact of D-alanylation of Lipoteichoic acid (LTA) on biofilm formation and acidogenesis of Streptococcus mutans (S. mutans). The research found that blocking the D-alanylation of LTA disrupts normal biofilm formation in S. mutans by altering intercellular auto-aggregation, cell adhesion, and extracellular matrix formation. It also suggests that LTA D-alanylation plays an important role in S. mutans acidogenesis by altering glycolytic activity.
MICROBIAL PATHOGENESIS
(2022)
Letter
Infectious Diseases
Bryan P. White, Emily A. Siegrist
Summary: The study mainly focuses on clinical forms of early-stage neurosyphilis and is not applicable to asymptomatic or late-stage neurosyphilis. There is an issue of unbalanced baseline characteristics in the study, which may indicate a potential bias.
LANCET INFECTIOUS DISEASES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Minjing Wu, Shan Huang, Jingyun Du, Shan Jiang, Zhiyu Cai, Ling Zhan, Xiaojing Huang
Summary: The D-alanylation of lipoteichoic acid plays a crucial role in regulating the physiological metabolism of Streptococcus mutans. This study demonstrates that the lack of D-alanylation in S. mutans leads to decreased production of antimicrobial compounds, compromised competitiveness in dual-species biofilms, and weaker demineralization of enamel in the presence of commensal bacteria. These findings contribute to a better understanding of dental caries prevention and treatment strategies.
MOLECULAR ORAL MICROBIOLOGY
(2021)
Article
Microbiology
Yutaka Koyano, Kiyoshirou Okajima, Mako Mihara, Hiroki Yamamoto
Summary: In Gram-positive bacteria, the cell wall is composed of mesh-like peptidoglycan and covalently linked wall teichoic acid (WTA). Nascent WTA decoration occurs in a patch-like manner at the peptidoglycan synthesis sites on the cytoplasmic membrane. The newly synthesized WTA is inserted into the cell wall layer and reaches the outermost layer of the cell wall after approximately half an hour. The incorporation of newly glucosylated WTA is inhibited by vancomycin but restored upon the removal of the antibiotic.
JOURNAL OF BACTERIOLOGY
(2023)
Article
Polymer Science
Kuen Hee Eom, Shuwei Li, Eun Gyeong Lee, Jae Ho Kim, Jung Rae Kim, Il Kim
Summary: Cell-penetrating peptides are facilitated by the presence of arginine or other guanidinium groups. However, little research has been done on the impact of these functional groups on antimicrobial peptides. In this study, a series of cationic linear, star, and multi-branched copolymers were synthesized and their antibacterial efficacy was tested. The results showed that the structure and composition of the copolymers significantly influenced their antibacterial activity, with the multi-branched structure exhibiting the highest efficacy.
Article
Chemistry, Physical
Fenfen Ma, Xiaoyan Zheng, Zesheng Li
Summary: The study using molecular dynamics simulations reveals that CB[7] binds differently to aromatic and basic residues in peptides, with the C-terminal basic residue significantly affecting the binding. Additionally, CB[7] shows efficient differentiation between peptides with similar structures, and exhibits sequence dependence in recognizing specific peptides.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Article
Medicine, General & Internal
Cameron M. Wright, Rachael Moorin, Glenn Pearson, John R. Dyer, Jonathan R. Carapetis, Laurens Manning
Summary: The incidence of invasive group A Streptococcus disease in Western Australia increased from 2000 to 2018, particularly among Indigenous Australians. Mandatory notification of invasive GAS disease is recommended to address this issue.
MEDICAL JOURNAL OF AUSTRALIA
(2021)
Article
Multidisciplinary Sciences
Jeffrey S. Rush, Prakash Parajuli, Alessandro Ruda, Jian Li, Amol Arunrao Pohane, Svetlana Zamakhaeva, Mohammad M. Rahman, Jennifer C. Chang, Artemis Gogos, Cameron W. Kenner, Gerard Lambeau, Michael J. Federle, Konstantin Korotkov, Goran Widmalm, Natalia Korotkova
Summary: The study characterizes the linkage between the polysaccharide GAC and peptidoglycan in the cell wall of Group A Streptococcus, a bacterial pathogen. It identifies a protein, PplD, that deacetylates the linkage and protects the pathogen against host cationic antimicrobial proteins.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Hugo Guerin, Saulius Kulakauskas, Marie-Pierre Chapot-Chartier
Summary: Rha-CWPSs are crucial cell wall components of many important bacteria, playing a critical role in bacterial growth, division, and interactions with phages or hosts. This review summarizes the current knowledge on the structure, biosynthesis, and contribution of Rha-CWPSs to cell wall architecture and biogenesis, as well as their importance in cell division.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Maria C. Sanchez, Angela Velapatino, Arancha Llama-Palacios, Alberto Valdes, Alejandro Cifuentes, Maria J. Ciudad, Luis Collado
Summary: The ecological dysbiosis of a biofilm involves changes in both bacteria and their metabolism. In the case of oral biofilms, changes in metabolic activity are directly related to the pathogenicity of oral diseases. A study used 16S rRNA gene sequencing and UHPLC/Q-TOF-MS analysis to explore the microbial and metabolomic etiology of caries. It found that sucrose triggers significant changes in the composition of the biofilm, leading to alterations in both the bacterial community and the extracellular metabolome.
MOLECULAR ORAL MICROBIOLOGY
(2022)
Article
Chemistry, Medicinal
Farjana Khatun, Charles C. Dai, Tania Rivera-Hernandez, Waleed M. Hussein, Zeinab G. Khalil, Robert J. Capon, Istvan Toth, Rachel J. Stephenson
Summary: This study evaluated the structure-immunogenicity relationship of carbohydrate moieties of a single repeating unit of group A carbohydrate on the cell wall of group A Streptococcus using a self-adjuvanted lipid-core peptide instead of a carrier protein. The construct with higher rhamnose moieties was found to induce enhanced immunogenic activity in mice, with GlcNAc moiety not essential for the immunogenic GAC mimicked epitope. The trirhamnosyl-lipopeptide also showed high opsonic activity against different clinical isolates of GAS, comparable to a subunit peptide vaccine.
ACS INFECTIOUS DISEASES
(2021)
Article
Biochemistry & Molecular Biology
Ildiko Szabo, Francoise Illien, Levente E. Dokus, Mo'ath Yousef, Zsuzsa Baranyai, Szilvia Bosze, Shoko Ise, Kenichi Kawano, Sandrine Sagan, Shiroh Futaki, Ferenc Hudecz, Zoltan Banoczi
Summary: Studies have shown that adding a Dabcyl group to tetraarginine and hexaarginine can increase their cellular uptake efficiency, with hexaarginine showing more significant effects. The modified hexaarginine may enter cells more effectively than octaarginine, and has shown good distribution even at low concentrations.
Article
Biochemistry & Molecular Biology
Vikrant Minhas, Arnau Domenech, Dimitra Synefiaridou, Daniel Straume, Max Brendel, Gonzalo Cebrero, Xue Liu, Charlotte Costa, Mara Baldry, Jean-Claude Sirard, Camilo Perez, Nicolas Gisch, Sven Hammerschmidt, Leiv Sigve Havarstein, Jan-Willem Veening
Summary: Competence development in Streptococcus pneumoniae controls genetic transformation, biofilm formation, and virulence. During competence, the genes involved in teichoic acid biosynthesis are essential. LytR and ComM play crucial roles in WTA synthesis and immunity against CbpD. Moreover, key virulence factors PspA and PspC become more surface-exposed at midcell during competence, in a CbpD-dependent manner.
Article
Chemistry, Inorganic & Nuclear
Hugh D. Glossop, Gayan Heruka De Zoysa, Lisa Pilkington, David Barker, Vijayalekshmi Sarojini
Summary: The synthesis and testing of a library of fluorinated 0-phenyl serine analogues showed enhanced broad-spectrum antimicrobial activity compared to commercial fluorinated phenylalanine amino acids. The most potent fluorinated peptide exhibited a 16-fold increase in antimicrobial activity against the fire blight pathogen, suggesting potential for future development as crop treatments.
JOURNAL OF FLUORINE CHEMISTRY
(2021)
Review
Microbiology
Laura Devaux, Pierre-Alexandre Kaminski, Patrick Trieu-Cuot, Arnaud Firon
CURRENT OPINION IN MICROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Giuseppe Valerio De Gaetano, Giampiero Pietrocola, Letizia Romeo, Roberta Galbo, Germana Lentini, Miriam Giardina, Carmelo Biondo, Angelina Midiri, Giuseppe Mancuso, Mario Venza, Isabella Venza, Arnaud Firon, Patrick Trieu-Cuot, Giuseppe Teti, Pietro Speziale, Concetta Beninati
MOLECULAR MICROBIOLOGY
(2018)
Article
Genetics & Heredity
Laura Devaux, Dona Sleiman, Maria-Vittoria Mazzuoli, Myriam Gominet, Philippe Lanotte, Patrick Trieu-Cuot, Pierre-Alexandre Kaminski, Arnaud Firon
Article
Multidisciplinary Sciences
Elva Bonifacio Andrade, Ana Magalhaes, Ana Puga, Madalena Costa, Joana Bravo, Camila Cabral Portugal, Adilia Ribeiro, Margarida Correia-Neves, Augusto Faustino, Arnaud Firon, Patrick Trieu-Cuot, Teresa Summavielle, Paula Ferreira
NATURE COMMUNICATIONS
(2018)
Article
Multidisciplinary Sciences
Germana Lentini, Angelina Midiri, Arnaud Firon, Roberta Galbo, Giuseppe Mancuso, Carmelo Biondo, Emanuela Mazzon, Annamaria Passantino, Letizia Romeo, Patrick Trieu-Cuot, Giuseppe Teti, Concetta Beninati
SCIENTIFIC REPORTS
(2018)
Article
Immunology
Bruno Perichon, Julie Guignot, Noemi Szili, Cherry Gao, Claire Poyart, Patrick Trieu-Cuot, Shaynoor Dramsi
MICROBES AND INFECTION
(2019)
Article
Immunology
Asmaa Tazi, Celine Plainvert, Olivia Anselem, Morgane Ballon, Valerie Marcou, Aurelien Seco, Fatma El Alaoui, Caroline Joubrel, Najoua El Helali, Emile Falloukh, Amandine Frigo, Josette Raymond, Patrick Trieu-Cuot, Catherine Branger, Alban Le Monnier, Elie Azria, Pierre-Yves Ancel, Pierre Henri Jarreau, Laurent Mandelbrot, Francois Goffinet, Claire Poyart
CLINICAL INFECTIOUS DISEASES
(2019)
Article
Immunology
Mariana Martins, Laurence du Merle, Patrick Trieu-Cuot, Shaynoor Dramsi
MICROBES AND INFECTION
(2020)
Editorial Material
Infectious Diseases
Catarina Magalhaes, Margarida Lima, Patrick Trieu-Cuot, Paula Ferreira
Summary: Sir Alexander Fleming warned against overuse of antibiotics in 1945, and evidence has shown that bacteria can become resistant to almost any available molecule. Prescribing fewer antibiotics and shorter treatment duration can be as effective as longer regimens, reducing selective pressure on microorganisms and delaying the emergence of resistant clones. Further studies are needed to identify optimal antibiotic therapy duration for common infections and clinical biomarkers for guiding antibiotic treatment.
LANCET INFECTIOUS DISEASES
(2021)
Article
Microbiology
Alexis Proutiere, Laurence du Merle, Bruno Perichon, Hugo Varet, Myriam Gominet, Patrick Trieu-Cuot, Shaynoor Dramsi
Summary: Bacteriocins are natural antimicrobial peptides produced by bacteria to kill competitors. Streptococcus gallolyticus subsp. gallolyticus outcompetes commensal enterococci in murine microbiota under tumoral conditions by producing a bacteriocin named gallocin. The regulatory control of gallocin involves a two-peptide system (BlpH/BlpR) activated by a secreted peptide (GSP), with BlpS repressing gallocin gene transcription.
Article
Public, Environmental & Occupational Health
Yu-Jin Jung, Bich-Tram Huynh, Abdoulaye Seck, Raymond Bercion, Fatoumata Diene Sarr, Perlinot Herindrainy, Jean-Baptiste Diouf, Zafitsara Zo Andrianirina, Arnaud Firon, Patrick Trieu-Cuot, Sophie Goyet, Jean-Marc Collard, Elisabeth Delarocque-Astagneau, Didier Guillemot, Muriel Vray
Summary: This study estimated the prevalence of Group B Streptococcus (GBS) colonization among pregnant women in Madagascar and Senegal, and found rates of 5.0% and 16.1% respectively. No sociodemographic characteristics, living conditions, or obstetric history were independently associated with GBS colonization in either country. This community-based study provided valuable information on maternal GBS colonization in these regions.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Genetics & Heredity
Maria-Vittoria Mazzuoli, Maelle Daunesse, Hugo Varet, Isabelle Rosinski-Chupin, Rachel Legendre, Odile Sismeiro, Myriam Gominet, Pierre Alexandre Kaminski, Philippe Glaser, Claudia Chica, Patrick Trieu-Cuot, Arnaud Firon
Summary: This study reveals the regulation of virulence genes in Group B Streptococcus by the master regulator CovR and emphasizes the importance of intra-species evolution in the emergence of specific disease-associated clones. The expression of specific surface proteins is linked to the hypervirulence of certain GBS clones.