Article
Multidisciplinary Sciences
Evgeny A. Shirshin, Marina V. Shirmanova, Alexey V. Gayer, Maria M. Lukina, Elena E. Nikonova, Boris P. Yakimov, Gleb S. Budylin, Varvara V. Dudenkova, Nadezhda I. Ignatova, Dmitry V. Komarov, Vladislav V. Yakovlev, Wolfgang Becker, Elena V. Zagaynova, Vladislav I. Shcheslavskiy, Marlan O. Scully
Summary: The heterogeneity of cells determines their response to external factors, and the metabolic heterogeneity of tumor cells is of clinical relevance. Fluorescence lifetime imaging (FLIM) is a non-invasive technique that can sense and quantify cellular metabolism by measuring the fluorescence decay parameters of endogenous fluorophores. This study demonstrates the unique capability of FLIM in accurately discriminating metabolic heterogeneity using a non-parametric analysis approach, which has been validated in cultured cancer cells treated with chemotherapy.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biotechnology & Applied Microbiology
Tiffany M. Heaster, Alexa R. Heaton, Paul M. Sondel, Melissa C. Skala
Summary: Metabolic autofluorescence imaging was used to assess cell-level macrophage heterogeneity in response to normal and cancerous tissue microenvironments in vivo. Tumor-associated macrophages exhibited higher oxidation levels compared to dermal macrophages, with changes in NAD(P)H and FAD protein-binding activities.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Plant Sciences
Raghav Kataria, Rakesh Kaundal
Summary: In this study, the protein-protein interactions between two races of Puccinia graminis that cause stem rust in wheat were analyzed. Computational methods were used to predict these interactions, and functional analysis revealed significant differences between the two pathogen races. Important host and pathogen proteins were identified and their roles in plant defense were elucidated.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Tongcheng Qian, Tiffany M. Heaster, Angela R. Houghtaling, Kexin Sun, Kayvan Samimi, Melissa C. Skala
Summary: The study presents a non-invasive label-free live cell imaging platform based on autofluorescence imaging to predict efficiency of hPSC differentiation into cardiomyocytes. This method provides high accuracy to separate differentiating cells within 1 day, avoiding variability in cell manufacturing.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Polina Vishnyakova, Elena Nikonova, Enar Jumaniyazova, Ilya Solovyev, Anastasia Kirillova, Maria Farmakovskaya, Alexander Savitsky, Evgeny Shirshin, Gennady Sukhikh, Timur Fatkhudinov
Summary: In this study, the metabolic status of spermatozoa from healthy donors was assessed using fluorescence lifetime imaging microscopy (FLIM). It was found that FLIM could be used to separate and group male germ cells based on their metabolic activity, which corresponded with the sperm motility measured in standard spermogram tests.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Review
Obstetrics & Gynecology
Marta Venturas, Xingbo Yang, Denny Sakkas, Dan Needleman
Summary: Discusses the major challenge in assisted reproductive technology (ART) of selecting high-quality oocytes and embryos, and proposes the potential utility of metabolic measurements in aiding selection. Reviews recent work on noninvasive metabolic imaging using fluorescence lifetime imaging microscopy (FLIM) of cumulus cells, oocytes, and embryos. Argues that FLIM provides quantitative information on NAD(P)H and FAD+ concentrations and engagement with enzymes, making it a promising approach for oocyte and embryo selection.
HUMAN REPRODUCTION
(2023)
Correction
Microbiology
Karthika Rajeeve, Nadine Vollmuth, Sudha Janaki-Raman, Thomas F. Wulff, Apoorva Baluapuri, Francesca R. Dejure, Claudia Huber, Julian Fink, Maximilian Schmalhofer, Werner Schmitz, Rajeeve Sivadasan, Martin Eilers, Elmar Wolf, Wolfgang Eisenreich, Almut Schulze, Jurgen Seibel, Thomas Rudel
Summary: The correction to this paper has been published.
NATURE MICROBIOLOGY
(2021)
Article
Microbiology
Nadja Kading, Nis Schmidt, Celeste Scholz, Simon Graspeuntner, Jan Rupp, Kensuke Shima
Summary: Research indicates that azithromycin (AZM) is less effective than doxycycline (DOX) in treating Chlamydia trachomatis infection, which may be related to the metabolic status of host cells. AZM fails to effectively suppress host cell metabolic pathways, leading to chlamydial reactivation and induced cytokine responses. Further studies are needed to elucidate its functional relevance and impact on disease progression.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Engineering, Environmental
Adrian Monteleone, Folker Wenzel, Heinz Langhals, Daniel Dietrich
Summary: The FLIM method was tested for identification and characterization of six types of plastics, showing significant differentiation in 94.55% of the comparisons. Results suggest that FLIM has the potential for sub-micrometer plastic characterization and allows for visual 3D-sectioning of samples, which could be important for identifying and characterizing plastics in tissue and environmental samples.
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2021)
Article
Biochemical Research Methods
P. Wang, F. Hecht, G. Ossato, S. Tille, S. E. Fraser, J. A. Junge
Summary: Fluorescence lifetime imaging microscopy (FLIM) with phasor analysis allows for easy visualization and analysis of fluorophores' lifetimes, but typically suffers from low photon budgets leading to poor signal to noise ratios. Traditional median filters used in phasor analysis degrade high spatial frequency FLIM information, especially edges and puncta. A new filtering strategy using complex wavelet filtering and Anscombe transform preserves fine structures and accurate lifetimes in photon starved FLIM imaging, outperforming median filters and enabling FLIM imaging with lower laser power and faster imaging speed.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Chemistry, Multidisciplinary
Peng Zhou, Jiawei Shen, Jun Liang, Tian Xue, Yuansheng Sun, Longhua Zhang, Changlin Tian
Summary: In this study, we established a two-photon fluorescence lifetime imaging microscopy platform and verified the feasibility and stability of NADH-based two-photon FLIM in paraformaldehyde-fixed mouse cerebral slices. We found that the metabolic state based on NADH showed oscillation in the suprachiasmatic nucleus (SCN), with lower free NADH levels during the daytime and ultradian oscillation at night.
CHINESE CHEMICAL LETTERS
(2023)
Article
Multidisciplinary Sciences
Gaurav Singh, Geen George, Sufi O. Raja, Ponnuvel Kandaswamy, Manoj Kumar, Shashi Thutupalli, Sunil Laxman, Akash Gulyani
Summary: We developed a robust method to visualize the fluidity of the inner mitochondrial membrane in living cells using a cell-permeable fluorescent molecular rotor. Our study revealed that the fluidity of the inner mitochondrial membrane is linked to cellular respiration and responsiveness to stimuli, and modulating this fluidity can support enhanced respiratory flux.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biotechnology & Applied Microbiology
Linghao Hu, Nianchao Wang, Joshua Bryant, Lin Liu, Linglin Xie, A. Phillip West, Alex Walsh
Summary: Autofluorescence lifetime imaging combined with machine learning models can detect metabolic perturbations between glycolysis and oxidative metabolism of living samples at a cellular level, providing a label-free technology to study cellular metabolism and metabolic heterogeneity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Martin Priessner, Peter A. Summers, Benjamin W. Lewis, Magdalena Sastre, Liming Ying, Marina K. Kuimova, Ramon Vilar
Summary: A new optical probe based on BODIPY has been developed, which exhibits fluorescence intensity switch-on upon binding to copper(I) and can be used to visualize copper(I) pools in lysosomes of live cells.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Obstetrics & Gynecology
Marta Venturas, Jaimin S. Shah, Xingbo Yang, Tim H. Sanchez, William Conway, Denny Sakkas, Dan J. Needleman
Summary: Fluorescence lifetime imaging microscopy (FLIM) can detect variations in metabolic profiles between discarded human blastocysts. The study revealed extensive metabolic variations associated with blastocyst development, as well as metabolic heterogeneity within individual blastocysts, suggesting FLIM as a promising approach for evaluating embryo viability.
HUMAN REPRODUCTION
(2022)
Article
Clinical Neurology
Joanne Trinh, Theresa Luth, Susen Schaake, Bjorn-Hergen Laabs, Kathleen Schlueter, Joshua Lass, Jelena Pozojevic, Ronnie Tse, Inke Koenig, Roland Dominic Jamora, Raymond L. Rosales, Norbert Brueggemann, Gerard Saranza, Cid Czarina E. Diesta, Frank J. Kaiser, Christel Depienne, Christopher E. Pearson, Ana Westenberger, Christine Klein
Summary: By sequencing the genomes of XDP patients, researchers have discovered various mutations within the repetitive sequence SINE-VNTR-Alu(AGAGGG)(n), which may act as modifiers of disease expression in XDP.
Article
Clinical Neurology
Armin Steffen, Florian Joffrey Moritz, Inke R. Koenig, Maria Suurna, Norbert Brueggemann
Summary: This study examined the physiological effect of unilateral hypoglossal nerve stimulation on bilateral tongue motion and found no significant difference in EMG signals based on different factors such as electrode configurations, stimulation-cuff position, body-mass-index, and sleep apnea severity. The findings highlight the importance of considering individual anatomy during therapy titration for optimal response.
JOURNAL OF SLEEP RESEARCH
(2023)
Article
Public, Environmental & Occupational Health
Alexander Balck, Bandik Foeh, Max Borsche, Johann Rahmoeller, Eva-Juliane Vollstedt, Frederike Waldeck, Nadja Kaeding, Christoph Twesten, Alexander Mischnik, Gabriele Gillessen-Kaesbach, Marc Ehlers, Christian Sina, Stefan Taube, Hauke Busch, Jan Rupp, Alexander Katalinic, Christine Klein
Summary: Considering the spread of new SARS-CoV-2 variants, low vaccination rates, and the risk of post-COVID syndrome, targeted intervention measures are urgently needed. The ELISA-Study is a longitudinal surveillance study that aims to study immunity, post-COVID-19 symptoms, and related risk factors through PCR testing, antibody measurements, and questionnaires. This study enables continuous monitoring of the pandemic and identification of potential long-term effects.
Article
Dermatology
Jasper N. Pruessmann, Ewan A. Langan, Jan Rupp, Jens Marquardt, Patrick Terheyden, Detlef Zillikens, Ralf J. Ludwig, Katharina Boch
Summary: This study aimed to evaluate the number of newly-discovered positive serological hepatitis B virus (HBV) test results in patients with autoimmune skin diseases after intravenous immunoglobulin (IVIg) treatment. The results showed that there were new positive serological HBV test results after IVIg treatment, which may be due to passive antibody transfer. It is recommended to screen for hepatitis B before IVIg therapy to prevent diagnostic confusion caused by passive antibody transfer.
JOURNAL OF DERMATOLOGY
(2022)
Review
Genetics & Heredity
Sophia Kappen, Daniele Bottigliengo, Amke Caliebe, Fabiola M. Del Greco, Inke R. Koenig
Summary: This systematic review suggests that most MR studies for PD were well-performed and can help identify causal exposures, which may inform further studies on the prevention and early intervention of PD.
MEDIZINISCHE GENETIK
(2022)
Article
Clinical Neurology
Joanne Trinh, Andrew A. Hicks, Inke R. Koenig, Sylvie Delcambre, Theresa Lueth, Susen Schaake, Kobi Wasner, Jenny Ghelfi, Max Borsche, Carles Vilarino-Gueell, Faycel Hentati, Elisabeth L. Germer, Peter Bauer, Masashi Takanashi, Vladimir Kostic, Anthony E. Lang, Norbert Brueggemann, Peter P. Pramstaller, Irene Pichler, Alex Rajput, Nobutaka Hattori, Matthew J. Farrer, Katja Lohmann, Hansi Weissensteiner, Patrick May, Christine Klein, Anne Gruenewald
Summary: Trinh et al. found that PINK1/PRKN mutations increase the risk of mitochondrial DNA variant accumulation in a dose- and disease-dependent manner. Monoallelic mutation carriers can be distinguished by the level of heteroplasmic mtDNA variants. Biallelic mutations in PINK1/PRKN lead to recessive Parkinson's disease.
Article
Multidisciplinary Sciences
Swapnil Prakash Doijad, Nicolas Gisch, Renate Frantz, Bajarang Vasant Kumbhar, Jane Falgenhauer, Can Imirzalioglu, Linda Falgenhauer, Alexander Mischnik, Jan Rupp, Michael Behnke, Michael Buhl, Simone Eisenbeis, Petra Gastmeier, Hanna Goelz, Georg Alexander Haecker, Nadja Kaeding, Winfried Kern, Axel Kola, Evelyn Kramme, Silke Peter, Anna M. Rohde, Harald Seifert, Evelina Tacconelli, Maria J. G. T. Vehreschild, Sarah Walker, Janine Zweigner, Dominik Schwudke, Trinad Chakraborty
Summary: A three-year study in Germany and a global analysis of isolates identified Enterobacter xiangfangensis as the most common species (65.5%) within the Enterobacter cloacae complex (ECC). The study revealed widespread resistance to colistin, with the mobile colistin resistance (mcr)-9 gene detected in 19.2% of all isolates. The resistance and heteroresistance properties were found to be dependent on the arnBCADTEF gene cassette, whose products catalyze transfer of L-Ara4N to lipid A.
NATURE COMMUNICATIONS
(2023)
Article
Pharmacology & Pharmacy
Anusha Balla, Bao Tran, Annika Valtari, Philipp Steven, Camilla Scarpellini, Koen Augustyns, Arto Urtti, Kati-Sisko Vellonen, Marika Ruponen
Summary: In this study, the positive effect of a novel ferroptosis inhibitor, UAMC-3203, on corneal epithelial wound healing was investigated using in vitro and ex vivo models. The drug solution at a concentration of 100 μM showed no toxicity when applied topically in rats for 5 days. Furthermore, the distribution of UAMC-3203 in the cornea revealed significantly higher concentration in the epithelium compared to the stroma.
Article
Infectious Diseases
Philipp Mathe, Siri Goepel, Daniel Hornuss, David Tobys, Nadja Kaeding, Simone Eisenbeis, Britta Kohlmorgen, Janina Trauth, Hanna Goelz, Sarah V. Walker, Alexander Mischnik, Silke Peter, Florian Hoelzl, Anna M. Rohde, Michael Behnke, Moritz Fritzenwanker, Georg Haecker, Benedict Steffens, Maria Vehreschild, Evelyn Kramme, Jane Falgenhauer, Gabriele Peyerl-Hoffmann, Harald Seifert, Jan Rupp, Petra Gastmeier, Can Imirzalioglu, Evelina Tacconelli, Winfried Kern, Siegbert Rieg
Summary: This study aims to describe the temporal trends, epidemiological characteristics, clinical manifestations, and outcomes of Staphylococcus aureus bloodstream infection (SAB). The findings showed an increasing number of SAB cases, an increase in community-acquired SAB, and a decrease in methicillin-resistant SAB. There was also an increase in risk factors for complicated/difficult-to-treat SAB and a higher level of comorbidities. The rate of deep-seated foci such as osteomyelitis or deep-seated abscesses significantly increased. In-hospital mortality decreased in patients with infectious diseases consultations.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Oncology
David Krug, Detlef Imhoff, Alfred Haidenberger, Nicole Hessler, Jane Schaefer, Stefan Huttenlocher, Georgios Chatzikonstantinou, Christoph Fuerweger, Ulla Ramm, Inke R. Koenig, Felix Chun, Michael Staehler, Claus Roedel, Alexander Muacevic, Reinhard Vonthein, Juergen Dunst, Oliver Blanck
Summary: This study aimed to evaluate the efficacy and toxicity of robotic stereotactic body radiotherapy (SBRT) for patients with localized prostate cancer. The results showed that robotic SBRT had minimal toxicity and produced favorable short-term outcomes.
STRAHLENTHERAPIE UND ONKOLOGIE
(2023)
Article
Microbiology
S. Graspeuntner, M. Lupatsii, L. Dashdorj, A. Rody, J. Rupp, V. Bossung, C. Haertel
Summary: The human gut microbiome is important for health and disease. Stool samples are commonly used for studying the gut microbiome, but alternative sampling approaches are needed due to difficulties in acquisition, transportation, and patient discomfort. This study compared rectal swabs and meconium paired samples collected from infants on the first days of life. The results indicate that rectal swabs are not a reliable proxy compared to stool samples for gut microbiome analysis in newborns.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Gabriela Rios Martini, Ekaterina Tikhonova, Elisa Rosati, Meghan Bialt Decelie, Laura Katharina Sievers, Florian Tran, Matthias Lessing, Arne Bergfeld, Sophia Hinz, Susanna Nikolaus, Julia Kuempers, Anna Matysiak, Philipp Hofmann, Carina Saggau, Stephan Schneiders, Ann-Kristin Kamps, Gunnar Jacobs, Wolfgang Lieb, Jochen Maul, Britta Siegmund, Barbara Seegers, Holger Hinrichsen, Hans-Heinrich Oberg, Daniela Wesch, Stefan Bereswill, Markus M. Heimesaat, Jan Rupp, Olaf Kniemeyer, Axel A. Brakhage, Sascha Brunke, Bernhard Hube, Konrad Aden, Andre Franke, Iliyan D. Iliev, Alexander Scheffold, Stefan Schreiber, Petra Bacher
Summary: Aberrant CD4(+) T cell reactivity against intestinal microorganisms is thought to drive mucosal inflammation in inflammatory bowel diseases. This study identifies yeasts as direct activators of altered CD4(+) T cell reactions in patients with Crohn's disease, revealing their potential role in chronic activation of inflammatory immune responses.
Article
Microbiology
Jerome Genth, Kathrin Schaefer, Liam Cassidy, Simon Graspeuntner, Jan Rupp, Andreas Tholey
Summary: Short open reading frames (sORFs) can encode small proteins called sORF-encoded peptides (SEP) that play important roles in various physiological processes. Using proteomics techniques, researchers identified 45 novel and previously reported SEPs in single cultures of Blautia producta under different growth conditions and stress exposure. The results suggest that the biosynthesis of specific SEPs is not limited to bacterial interactions within the microbiome but is influenced by growth and environmental conditions during cultivation.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Hanna Marti, Michael Biggel, Kensuke Shima, Delia Onorini, Jan Rupp, Steve J. Charette, Nicole Borel
Summary: Chlamydia, a genus of bacteria, has obligate intracellular lifestyle which limits genetic exchange. The only known example of horizontal gene transfer (HGT) between different phyla in Chlamydia is the tetracycline resistance found in Chlamydia suis.
MICROBIOLOGY SPECTRUM
(2023)
Review
Respiratory System
Mathias W. Pletz, Andreas Vestergaard Jensen, Christina Bahrs, Claudia Davenport, Jan Rupp, Martin Witzenrath, Grit Barten-Neiner, Martin Kolditz, Sabine Dettmer, James D. Chalmers, Daiana Stolz, Norbert Suttorp, Stefano Aliberti, Wolfgang M. Kuebler, Gernot Rohde
Summary: Despite advances in CAP research, there are still many unmet needs. CAPNETZ identified eleven topics for future CAP research, including detection of causative pathogens, next generation sequencing for antimicrobial treatment guidance, imaging diagnostics, biomarkers, risk stratification, antiviral and antibiotic treatment, adjunctive therapy, vaccines and prevention, systemic and local immune response, comorbidities, and long-term cardio-vascular complications.
RESPIRATORY RESEARCH
(2022)
