Article
Multidisciplinary Sciences
Stefania Dispinseri, Massimiliano Secchi, Maria Franca Pirillo, Monica Tolazzi, Martina Borghi, Cristina Brigatti, Maria Laura De Angelis, Marco Baratella, Elena Bazzigaluppi, Giulietta Venturi, Francesca Sironi, Andrea Canitano, Ilaria Marzinotto, Cristina Tresoldi, Fabio Ciceri, Lorenzo Piemonti, Donatella Negri, Andrea Cara, Vito Lampasona, Gabriella Scarlatti
Summary: The study reveals that antibody responses to SARS-CoV-2 spike protein play a significant role in neutralization and protection against severe COVID-19, unaffected by heterologous boosting or common cold immunity, and can last for up to 8 months.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Mayuko Yagi, Minami Hama, Sayaka Ichii, Yurie Nakashima, Daiki Kanbayashi, Takako Kurata, Kosuke Yusa, Jun Komano
Summary: This study used CRISPR-Cas9 system to conduct a loss-of-function screening in human placenta-derived JAR cells, and identified sphingomyelin synthase 1 as a susceptibility factor for rubella virus infection. The study revealed the unique regulation of rubella virus replication by a host factor during the early and late phases of the viral life cycle.
Article
Biochemistry & Molecular Biology
Ruofan Wang, Camille R. Simoneau, Jessie Kulsuptrakul, Mehdi Bouhaddou, Katherine A. Travisano, Jennifer M. Hayashi, Jared Carlson-Stevermer, James R. Zengel, Christopher M. Richards, Parinaz Fozouni, Jennifer Oki, Lauren Rodriguez, Bastian Joehnk, Keith Walcott, Kevin Holden, Anita Sil, Jan E. Carette, Nevan J. Krogan, Melanie Ott, Andreas S. Puschnik
Summary: The study identified common host factors for three coronaviruses and critical host pathways supporting infection by all of them, including phosphatidylinositol phosphate biosynthesis and cholesterol homeostasis. Inhibition of these pathways reduced replication of all three coronaviruses, while the lysosomal protein TMEM106B appeared unique to SARS-CoV-2 infection. These findings provide important insights for understanding the coronavirus life cycle and developing host-directed therapies.
Article
Microbiology
Katherine I. Young, Federico Valdez, Christina Vaquera, Carlos Campos, Lawrence Zhou, Helen K. Vessels, J. Kevin Moulton, Barbara S. Drolet, Paula Rozo-Lopez, Angela M. Pelzel-McCluskey, Debra C. Peters, Luis L. Rodriguez, Kathryn A. Hanley
Summary: VSV periodically crosses the border from southern Mexico to cause outbreaks in the US, with black flies along the Rio Grande in New Mexico being identified as vectors carrying the virus. The study found a correlation between precipitation, temperature, vegetation, and black fly abundance, contributing to a better understanding of VSV transmission.
Article
Multidisciplinary Sciences
Nishi R. Sharma, Kundlik Gadhave, Prateek Kumar, Mohammad Saif, Md. M. Khan, Debi P. Sarkar, Vladimir N. Uversky, Rajanish Giri
Summary: CHPV, an emerging pathogen causing acute encephalitis in humans, exhibits a high level of disorder in its proteins, particularly in the Phosphoprotein (P), which could serve as potential targets for drug design. The presence of disorder-based binding regions in CHPV proteins suggests their importance in biological processes and viral life cycles.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Jingyuan Cong, Xiaoying Feng, Huiling Kang, Wangjun Fu, Lei Wang, Chenlong Wang, Xuemei Li, Yutao Chen, Zihe Rao
Summary: This study investigates the structure-function relationship of the L-P complex of Newcastle disease virus (NDV) using cryo-EM and enzymatic assays. The research reveals distinct conformations and interactions between L and P proteins, advancing our understanding of Paramyxoviridae RNA synthesis and providing potential therapeutic targets.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Delphine C. Malherbe, Drishya Kurup, Christoph Wirblich, Adam J. Ronk, Chad Mire, Natalia Kuzmina, Noor Shaik, Sivakumar Periasamy, Matthew A. Hyde, Julie M. Williams, Pei-Yong Shi, Matthias J. Schnell, Alexander Bukreyev
Summary: The study developed a SARS-CoV-2 ConVac vaccine which, through animal experiments, showed that a single dose of the vaccine could rapidly control viral replication after disease challenge, protecting animals from weight loss and lung virus replication, with some inflammation still detected.
Article
Biochemistry & Molecular Biology
Jaewon Song, Ryan D. Chow, Mario A. Pena-Hernandez, Li Zhang, Skylar A. Loeb, Eui-Young So, Olin D. Liang, Ping Ren, Sidi Chen, Craig B. Wilen, Sanghyun Lee
Summary: LRRC15 is identified as an inhibitory attachment factor for SARS-CoV-2 entry, which directly binds to the receptor-binding domain of spike protein and inhibits viral entry. It is mainly expressed in fibroblasts, particularly in pathological fibroblasts in COVID-19 patients. Expression of LRRC15 in ACE2-negative cells blocks spike-mediated viral entry in ACE2+ cells, suggesting a protective role of LRRC15 in a physiological context.
Article
Immunology
Daisy A. Hoagland, Rasmus Moller, Skyler A. Uhl, Kohei Oishi, Justin Frere, Ilona Golynker, Shu Horiuchi, Maryline Panis, Daniel Blanco-Melo, David Sachs, Knarik Arkun, Jean K. Lim, Benjamin R. tenOever
Summary: This study investigated the systemic response to SARS-CoV-2 infection in hamsters and found that inflammation was present in all tissues but not enough to prevent disease progression. Bolstering the antiviral response with intranasal administration of recombinant IFN-I reduced viral disease, prevented transmission, and lowered inflammation in vivo. This suggests that intranasal IFN-I could be an effective early treatment for SARS-CoV-2 infection.
Article
Biochemistry & Molecular Biology
Xing Liu, Fang Li, Jiwen Zhang, Lulu Wang, Jinliang Wang, Zhiyuan Wen, Zilong Wang, Lei Shuai, Xijun Wang, Jinying Ge, Dongming Zhao, Zhigao Bu
Summary: The study demonstrates that ATP6V1A interacts with RABV M protein to promote virus replication and is involved in viral uncoating process through specific residue interactions. Upregulation of ATP6V1A enhances RABV growth in cells.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Microbiology
Afzaal M. Shareef, Barbara Ludeke, Paul Jordan, Jerome Deval, Rachel Fearns
Summary: The study explores the RNA synthesis initiation properties of polymerases from three different non-segmented negative strand RNA virus families, revealing that they are fundamentally similar in their initiation properties but differ in their ability to engage in back-priming activity. This suggests potential structural differences between polymerases from different virus families and highlights the importance of understanding the similarities and differences in developing antiviral drugs with broad efficacy.
Article
Biochemistry & Molecular Biology
Tianle Gu, Dandan Yu, Ling Xu, Yu-Lin Yao, Xiao Zheng, Yong-Gang Yao
Summary: Tree shrew GBP1 was shown to exhibit antiviral activity against VSV infection by competing with the VSV nucleocapsid protein in binding to the VSV phosphoprotein, thereby repressing the primary transcription of the VSV genome.
Article
Virology
Basavaraj Binjawadagi, Yuanmei Ma, Rashmi Binjawadagi, Kelsey Brakel, Olivia Harder, Mark Peeples, Jianrong Li, Stefan Niewiesk
Summary: RSV remains a significant cause of lower respiratory tract infections, especially in high-risk populations. Vaccine candidates utilizing rVSV vectors expressing RSV surface proteins have shown promising results in inducing protective immunity against RSV infection, with mucosal delivery and prime-boost immunization regimen enhancing efficacy. Antibody response was found to be the main immune parameter responsible for protection of both upper and lower respiratory tracts.
JOURNAL OF VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Annette Runge, Melissa Mayr, Theresa Schwaiger, Susanne Sprung, Paolo Chetta, Timo Gottfried, Jozsef Dudas, Maria C. Greier, Marlies C. Glatz, Johannes Haybaeck, Knut Elbers, Herbert Riechelmann, Patrik Erlmann, Monika Petersson
Summary: The etiology and architecture of head and neck cancer are diverse and complex, making it difficult to predict patient responses to specific cancer immunotherapy or combination treatment. To bridge this gap, researchers have developed a patient-derived HNSCC (head and neck squamous cell carcinoma) slice culturing system. This system preserves the heterogeneous contexture of the human tumor ecosystem and functional immune cell compartment, allowing for assessment of immunomodulatory effects and oncolytic virus action.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Bin Yuan, Qi Peng, Jinlong Cheng, Min Wang, Jin Zhong, Jianxun Qi, George F. Gao, Yi Shi
Summary: Filoviruses, including Ebola virus, pose an increasing threat to public health. The structure of the Ebola virus L protein in complex with VP35 has been determined, revealing potential targets for anti-filovirus drugs. Additionally, suramin has been found to inhibit the activity of the Ebola virus polymerase.
Article
Multidisciplinary Sciences
Sergio E. Rodriguez, Robert W. Cross, Karla A. Fenton, Dennis A. Bente, Chad E. Mire, Thomas W. Geisbert
SCIENTIFIC REPORTS
(2019)
Correction
Multidisciplinary Sciences
Krista Versteeg, Andrea R. Menicucci, Courtney Woolsey, Chad E. Mire, Joan B. Geisbert, Robert W. Cross, Krystle N. Agans, Daniel Jeske, Ilhem Messaoudi, Thomas W. Geisbert
SCIENTIFIC REPORTS
(2019)
Article
Medicine, Research & Experimental
Kathleen M. McAndrews, Dara P. Dowlatshahi, Jianli Dai, Lisa M. Becker, Janine Hensel, Laura M. Snowden, Jennifer M. Leveille, Michael R. Brunner, Kylie W. Holden, Nikolas S. Hopkins, Alexandria M. Harris, Jerusha Kumpati, Michael A. Whitt, J. Jack Lee, Luis L. Ostrosky-Zeichner, Ramesha Papanna, Valerie S. LeBleu, James P. Allison, Raghu Kalluri
Article
Cardiac & Cardiovascular Systems
Jose Manuel Condor Capcha, Guerline Lambert, Derek M. Dykxhoorn, Alessandro G. Salerno, Joshua M. Hare, Michael A. Whitt, Savita Pahwa, Dushyantha T. Jayaweera, Lina A. Shehadeh
Summary: Studying the viral entry mechanisms of SARS-CoV-2 is crucial for developing therapeutic approaches for COVID-19, and a simple and effective method has been developed for producing pseudoviruses carrying the SARS-CoV-2 spike, suitable for studying viral infection in various cell lines.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ha V. Dang, Robert W. Cross, Viktoriya Borisevich, Zachary A. Bornholdt, Brandyn R. West, Yee-Peng Chan, Chad E. Mire, Sofia Cheliout Da Silva, Antony S. Dimitrov, Lianying Yan, Moushimi Amaya, Chanakha K. Navaratnarajah, Larry Zeitlin, Thomas W. Geisbert, Christopher C. Broder, David Veesler
Summary: The research describes two cross-reactive F-specific antibodies, 1F5 and 12B2, that neutralize NiV and HeV through inhibition of membrane fusion by recognizing distinct prefusion-specific epitopes. The structural and functional characterization of these antibodies demonstrate the therapeutic potential of using antibody cocktails to protect against henipavirus infection. This study defines a roadmap for developing effective countermeasures against highly pathogenic viruses.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2021)
Article
Cell Biology
Taylor B. Engdahl, Natalia A. Kuzmina, Adam J. Ronk, Chad E. Mire, Matthew A. Hyde, Nurgun Kose, Matthew D. Josleyn, Rachel E. Sutton, Apoorva Mehta, Rachael M. Wolters, Nicole M. Lloyd, Francisca R. Valdivieso, Thomas G. Ksiazek, Jay W. Hooper, Alexander Bukreyev, James E. Crowe
Summary: The study found that the human response to New World hantaviruses infection has a convergent and potently neutralizing antibody reaction, with some monoclonal antibodies capable of broadly recognizing and cross-neutralizing both New and Old World hantaviruses. These antibodies hold therapeutic potential against HCPS.
Article
Microbiology
Debora Stelitano, Stuart P. Weisberg, Monica P. Goldklang, Yun Zhu, Francesca T. Bovier, Gavreel F. Kalantarov, Giuseppe Greco, Didier Decimo, Gianluigi Franci, Michele Cennamo, Giuseppe Portella, Massimiliano Galdiero, Cyrille Mathieu, Branka Horvat, Ilya N. Trakht, Anne Moscona, Michael A. Whitt, Matteo Porotto
Summary: This study introduces a new platform strategy for assessing virus-neutralizing activity and a cell-based immunofluorescent assay to accurately measure the neutralization potential of antibodies, providing useful tools for evaluating antiviral immunity acquired through natural infection or vaccination.
Article
Immunology
Delphine C. Malherbe, Drishya Kurup, Christoph Wirblich, Adam J. Ronk, Chad Mire, Natalia Kuzmina, Noor Shaik, Sivakumar Periasamy, Matthew A. Hyde, Julie M. Williams, Pei-Yong Shi, Matthias J. Schnell, Alexander Bukreyev
Summary: The study developed a SARS-CoV-2 ConVac vaccine which, through animal experiments, showed that a single dose of the vaccine could rapidly control viral replication after disease challenge, protecting animals from weight loss and lung virus replication, with some inflammation still detected.
Article
Microbiology
Nicholas Wohlgemuth, Kendall Whitt, Sean Cherry, Ericka Kirkpatrick Roubidoux, Chun-Yang Lin, Kim J. Allison, Ashleigh Gowen, Pamela Freiden, E. Kaitlynn Allen, Aditya H. Gaur, Jeremie H. Estepp, Li Tang, Tomi Mori, Diego R. Hijano, Hana Hakim, Maureen A. McGargill, Florian Krammer, Michael A. Whitt, Joshua Wolf, Paul G. Thomas, Stacey Schultz-Cherry
Summary: This study evaluated the correlation of six serological assays with authentic virus neutralization for SARS-CoV-2. The results showed that luciferase- and SEAP-expressing pseudotyped virus neutralizations were the most accurate in predicting authentic virus neutralization, followed by GFP-expressing pseudotyped virus neutralization, and then ELISAs. This research outlines the advantages and disadvantages of different assays, providing insights into the optimal surrogate assay for authentic virus neutralization and enhancing the accuracy of assessing protective immunity against SARS-CoV-2 post infection and vaccination.
MICROBIOLOGY SPECTRUM
(2021)
Article
Immunology
Philipp A. Ilinykh, Sivakumar Periasamy, Kai Huang, Natalia A. Kuzmina, Palaniappan Ramanathan, Michelle N. Meyer, Chad E. Mire, Ivan V. Kuzmin, Preeti Bharaj, Jessica R. Endsley, Maria Chikina, Stuart C. Sealfon, Steven G. Widen, Mark A. Endsley, Alexander Bukreyev
Summary: Respiratory tract vaccination induces immune responses at the portal of SARS-CoV-2 entry, including neutralizing antibodies and memory T cell responses, providing advantages against COVID-19.
Article
Biochemistry & Molecular Biology
Sajedah M. Hindi, Michael J. Petrany, Elena Greenfeld, Leah C. Focke, Alyssa A. W. Cramer, Michael A. Whitt, Ramzi J. Khairallah, Christopher W. Ward, Jeffrey S. Chamberlain, Benjamin Podbilewicz, Vikram Prasad, Douglas P. Millay
Summary: The muscle-specific fusogens Myomaker and Myo-merger can replace viral fusogens to mediate membrane fusion and deliver therapeutic genes to skeletal muscle. By engineering these muscle fusogens onto enveloped viruses, targeted transduction of skeletal muscle is achieved, providing a potential treatment for Duchenne muscular dystrophy.
Article
Entomology
Seth Gibson, Leela E. Noronha, Heidi Tubbs, Lee W. Cohnstaedt, William C. Wilson, Chad Mire, Dana Mitzel, Assaf Anyamba, Melinda Rostal, Kenneth J. Linthicum, Ary Faraji
Summary: Rift Valley fever virus (RVFV) is a vector-borne zoonotic disease that poses a threat to global agriculture and public health. It can be transmitted by mosquitoes and direct contact with infected tissues. With changing climate and environmental factors, the virus may be introduced to new areas. However, understanding the virus's transmission patterns and evolutionary factors can help reduce the risk of outbreaks.
JOURNAL OF MEDICAL ENTOMOLOGY
(2023)
Article
Multidisciplinary Sciences
Adam J. Ronk, Nicole M. Lloyd, Min Zhang, Caroline Atyeo, Hailee R. Perrett, Chad E. Mire, Kathryn M. Hastie, Rogier W. Sanders, Philip J. M. Brouwer, Erica Olmann Saphire, Andrew B. Ward, Thomas G. Ksiazek, Juan Carlos Alvarez Moreno, Harshwardhan M. Thaker, Galit Alter, Sunny Himansu, Andrea Carfi, Alexander Bukreyev
Summary: The study presents an mRNA-based Lassa virus vaccine that provides protection against lethal infection in a guinea pig model without the need for neutralizing antibodies. Vaccination induced strong binding antibody responses and demonstrated the potential of mRNA vaccines as a platform against Lassa virus.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Heather N. Grome, Michael Threlkeld, Steve Threlkeld, Charles Newman, Roosecelis Brasil Martines, Sarah Reagan-Steiner, Michael A. Whitt, Maria Gomes-Solecki, Nisha Nair, Mary-Margaret Fill, Timothy F. Jones, William Schaffner, John Dunn
Summary: This case describes a fatal instance of multisystem inflammatory syndrome in an adult occurring 22 days after receiving the second dose of an mRNA coronavirus vaccine. Both serologic and clinical findings suggested the infection of SARS-CoV-2 happened before the vaccination. The immunopathology of this syndrome, regardless of vaccination status, is still poorly understood.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Medicine, Research & Experimental
Michelle Meyer, Yuan Wang, Darin Edwards, Gregory R. Smith, Aliza B. Rubenstein, Palaniappan Ramanathan, Chad E. Mire, Colette Pietzsch, Xi Chen, Yongchao Ge, Wan Sze Cheng, Carole Henry, Angela Woods, LingZhi Ma, Guillaume B. E. Stewart-Jones, Kevin W. Bock, Mahnaz Minai, Bianca M. Nagata, Sivakumar Periasamy, Pei-Yong Shi, Barney S. Graham, Ian N. Moore, Irene Ramos, Olga G. Troyanskaya, Elena Zaslavsky, Andrea Carfi, Stuart C. Sealfon, Alexander Bukreyev
Summary: The study demonstrates that the mRNA-1273 vaccine is effective in hamsters against SARS-CoV-2, inducing robust neutralizing antibodies and reducing disease severity. It also shows the impact of the vaccine on immune cell infiltration, lymphocyte proportion, and antiviral responses conducive to lung health.
JOURNAL OF CLINICAL INVESTIGATION
(2021)