Article
Chemistry, Medicinal
Vladimir Sladek, Pavel Smak, Igor Tvarosska
Summary: This study explores essential interactions of selectin-ligand binding using a multiscale approach, successfully reproducing most of the experimental findings. The results enable us to quantify the importance of specific residues and functional groups at the protein-ligand interface and identify differences in molecular recognition by the three selectins. Mutations in the E-, L-, and P-selectins, particularly at residues 46, 85, 97, and 107, play a crucial role in ligand engagement. The sulfation of tyrosine residues in PSGL-1, specifically the interaction between TyrSO3- at position 51 and Arg85 in P-selectin, is a significant factor in the increased affinity of P-selectin to PSGL-1 compared to E- and L-selectins. Based on the analysis of selectin-PSGL-1 interactions, a pharmacophore targeting five essential PSGL-binding sites is proposed.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Virology
Dong Guo, Xinghai Yu, Dan Wang, Zhifei Li, Yu Zhou, Guodong Xu, Bing Yuan, Yali Qin, Mingzhou Chen
Summary: This study used CRISPR-Cas9 genetic screening to investigate the interaction between host cells and EV71. The results revealed the importance of SLC35B2 in regulating cellular sulfation, which affects the binding and internalization of EV71. Additionally, the study identified the involvement of SCARB2 and heparan sulfate in EV71 entry.
JOURNAL OF VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yuta Hasegawa, Sayaka Tonsho, Taiyo Nakazawa, Tadashi Kainuma, Kaori Kagasaki, Shota Sakurai, Kouki Kitagawa, Shinichi Asada
Summary: Tyrosylprotein sulfotransferases (TPSTs) transfer sulphonate moiety to tyrosine residues in substrate proteins. The minimum recognition chain length required for tyrosine sulfation remains unclear. This study shows that the ADY amino acid sequence can be sulfated by TPST1 and TPST2, and suggests that proteins with the ADY sequence may be useful for searching novel TPST tyrosine sulfated substrates.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Oncology
Julia M. DeRogatis, Karla M. Viramontes, Emily N. Neubert, Monique L. Henriquez, Christian F. Guerrero-Juarez, Roberto Tinoco
Summary: Therapeutically targeting PSGL-1 can reinvigorate antitumor immunity by enhancing effector T-cell responses and reducing regulatory T cells, thus delaying tumor growth in a melanoma mouse model.
CANCER IMMUNOLOGY RESEARCH
(2022)
Article
Multidisciplinary Sciences
Leike Zhang, Xuefang Peng, Qingxing Wang, Jin Li, Shouming Lv, Shuo Han, Lingyu Zhang, Heng Ding, Cong-Yi Wang, Gengfu Xiao, Xuguang Du, Ke Peng, Hao Li, Wei Liu
Summary: Severe fever with thrombocytopenia syndrome virus (SFTSV) is a tick-borne bunyavirus with a high fatality rate. C-C motif chemokine receptor 2 (CCR2) was identified as the host receptor for SFTSV, and its knockout reduced viral binding and infection. CCR2 enhanced SFTSV binding through direct binding to SFTSV glycoprotein N (Gn), and individuals with higher CCR2 surface expression showed stronger SFTSV binding and replication. CCR2 is a potential target for developing anti-SFTSV therapeutics.
Review
Virology
Tomoyuki Murakami, Akira Ono
Summary: During assembly, nascent HIV-1 particles incorporate host transmembrane proteins, which play crucial roles in virus spread. These host proteins may act as either pro-viral factors or anti-viral proteins. The functions of some host proteins in CD4(+) T cells have also been elucidated.
Article
Immunology
Ping-Ping Sun, Dan Li, Meng Su, Qing Ren, Wen-Ping Guo, Jiang-Li Wang, Luan-Ying Du, Guang-Cheng Xie
Summary: This study investigated the effects of TLR1/2/4/6 monomers and TLR2 heterodimer on EV71 replication and innate immune activation. It was found that TLR monomers and TLR2 heterodimer inhibited EV71 replication and induced the production of IL-8. Additionally, the EV71 capsid proteins served as pathogen-associated molecular patterns for TLR monomers and TLR2 heterodimer, activating innate immunity.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Roberto Tinoco, Emily N. Neubert, Christopher J. Stairiker, Monique L. Henriquez, Linda M. Bradley
Summary: PSGL-1 acts as a key regulator of effector and memory T cell differentiation during acute viral infection, by limiting survival, sustaining PD-1 expression, and reducing effector responses. This suggests that PSGL-1 may limit excessive stimulation of memory T cells during acute viral infection.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Yiming Yin, Brian D. Quinlan, Tianling Ou, Yan Guo, Wenhui He, Michael Farzan
Summary: Three variable 2 (V2) loops of HIV-1 envelope glycoprotein (Env) trimer converge at the Env apex to form the epitope of an important classes of HIV-1 broadly neutralizing antibodies (bNAbs). Improvements in the breadth and potency of the VRC26.25 antibody were achieved through B cell display approach, introducing mutations in the CDRH3 region. This study describes a broader and more potent form of VRC26.25 and a useful approach for enhancing the breadth and potency of antibodies with important posttranslational modifications.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Lars Krogvold, Ida Maria Mynarek, Erica Ponzi, Freja Barrett Mork, Trine Witzner Hessel, Trine Roald, Nina Lindblom, Jacob Westman, Peter Barker, Heikki Hyoety, Johnny Ludvigsson, Kristian F. Hanssen, Jesper Johannesen, Knut Dahl-Jorgensen
Summary: The results suggest that antiviral treatment may help preserve insulin production in children and adolescents with new-onset type 1 diabetes.
Article
Chemistry, Physical
Pavel Smak, Igor Tvaroska, Jaroslav Koca
Summary: Tyrosine sulfation can alter the biological activity of many proteins involved in different physiological and pathophysiological conditions. The study investigated the catalytic mechanism of isoform TPST-1 using QM/MM Car-Parrinello metadynamics simulations and potential energy calculations, revealing a S(N)2 type of catalytic mechanism. Understanding the reaction mechanism of TPST-1 may aid in the rational design of transition-state TPST inhibitors.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2021)
Review
Endocrinology & Metabolism
Kan Wang, Fei Ye, Yong Chen, Jianxin Xu, Yufang Zhao, Yeping Wang, Tian Lan
Summary: This meta-analysis suggests that there is an association between enterovirus infection and type 1 diabetes (T1D). The association was observed in various populations and sample types, with blood and tissue samples showing a correlation while stool samples did not.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Review
Public, Environmental & Occupational Health
Sen Yang, BiYing Zhao, Zhen Zhang, XiaoLin Dai, YiLi Zhang, LanWei Cui
Summary: The study found a significant association between EV infection and clinical type 1 diabetes mellitus, with statistical heterogeneity mainly attributed to factors such as methods of EV detection and age distribution.
EPIDEMIOLOGY AND INFECTION
(2021)
Review
Medicine, General & Internal
Adam J. O'Neal, Maureen R. Hanson
Summary: ME/CFS is a complex and poorly understood disease, with research supporting a potential link to enterovirus infections, though inconsistent results and methodological limitations persist. Further studies using the latest molecular methods are urgently needed to explore the potential impact of chronic enterovirus infections on ME/CFS.
FRONTIERS IN MEDICINE
(2021)
Article
Endocrinology & Metabolism
Wei-Liang Shih, Yi-Ching Tung, Luan-Yin Chang, Chi-Tai Fang, Wen-Yu Tsai
Summary: In Taiwan, the incidence of T1D increased in children aged 0-6 years but decreased in adolescents aged 13-19 years. Children aged 0-6 years with enterovirus infection had a significantly higher risk of T1D than noninfected subjects. The incidence of T1D in children aged 0-6 years was significantly correlated with the isolation rates of coxsackievirus A species.
Article
Clinical Neurology
Akihisa Okumura, Harushi Mori, Pin Fee Chong, Ryutaro Kira, Hiroyuki Torisu, Sawa Yasumoto, Hiroyuki Shimizu, Tsuguto Fujimoto, Keiko Tanaka-Taya
BRAIN & DEVELOPMENT
(2019)
Article
Immunology
Hiroshi Satoh, Keiko Tanaka-Taya, Hiroyuki Shimizu, Akiko Goto, Shizuka Tanaka, Tsuyoshi Nakano, Chiemi Hotta, Terue Okazaki, Masae Itamochi, Miyabi Ito, Reiko Okamoto-Nakagaw, Yasutaka Yamashita, Satoru Arai, Hideo Okuno, Saeko Morino, Kazunori Oishi
Article
Public, Environmental & Occupational Health
Mathias Bauri, Amanda L. Wilkinson, Berry Ropa, Keith Feldon, Cynthia J. Snider, Abhijeet Anand, Graham Tallis, Liliane Boualam, Varja Grabovac, Tigran Avagyan, Mohammad S. Reza, Dessie Mekonnen, Zaixing Zhang, Bruce R. Thorley, Hiroyuki Shimizu, Lea Necitas G. Apostol, Yoshihiro Takashima
MMWR-MORBIDITY AND MORTALITY WEEKLY REPORT
(2019)
Article
Public, Environmental & Occupational Health
Maria Takechi, Wakaba Fukushima, Takashi Nakano, Miki Inui, Satoko Ohfuji, Tetsuo Kase, Kazuya Ito, Kyoko Kondo, Akiko Maeda, Hiroyuki Shimizu, Yoshio Hirota
JOURNAL OF EPIDEMIOLOGY
(2019)
Article
Infectious Diseases
Ngan Thi Kim Pham, Aksara Thongprachum, Yuko Shimizu, Quang Duy Trinh, Shoko Okitsu, Shihoko Komine-Aizawa, Hiroyuki Shimizu, Satoshi Hayakawa, Hiroshi Ushijima
INFECTION GENETICS AND EVOLUTION
(2019)
Article
Clinical Neurology
Waka Ushioda, Osamu Kotani, Kengo Kawachi, Naoko Iwata-Yoshikawa, Tadaki Suzuki, Hideki Hasegawa, Hiroyuki Shimizu, Kimimasa Takahashi, Noriyo Nagata
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY
(2020)
Article
Virology
Thi Nguyen Hoa-Tran, Anh The Nguyen, Anh Thi Hai Dao, Chikako Kataoka, Hong Thi Thu Ta, Ha Thi Viet Nguyen, Taichiro Takemura, Trang Thi Thu Nguyen, Hung Manh Vu, Thanh Thi Hien Nguyen, Hiroyuki Shimizu
ARCHIVES OF VIROLOGY
(2020)
Article
Infectious Diseases
Shoko Okitsu, Pattara Khamrin, Sayaka Takanashi, Aksara Thongprachum, Sheikh Ariful Hoque, Haruko Takeuchi, Md Alfazal Khan, S. M. Tafsir Hasan, Tsutomu Iwata, Hiroyuki Shimizu, Masamine Jimba, Satoshi Hayakawa, Niwat Maneekarn, Hiroshi Ushijima
INFECTION GENETICS AND EVOLUTION
(2020)
Article
Immunology
Thi Nguyen Hoa-Tran, Anh Thi Hai Dao, Anh The Nguyen, Chikako Kataoka, Taichiro Takemura, Chau Ha Pham, Hung Manh Vu, Ta Thi Thu Hong, Nguyen Thi Viet Ha, Tran Nhu Duong, Nguyen Thi Hien Thanh, Hiroyuki Shimizu
Article
Infectious Diseases
Takako Utsumi, Maria Inge Lusida, Zayyin Dinana, Rury Mega Wahyuni, Soegeng Soegijanto, Soetjipto, Alpha Fardah Athiyyah, Subijanto Marto Sudarmo, Reza Gunadi Ranuh, Andy Darma, Juniastuti, Laura Navika Yamani, Yen Hai Doan, Hiroyuki Shimizu, Koji Ishii, Chieko Matsui, Lin Deng, Takayuki Abe, Kazuhiko Katayama, Ikuo Shoji
Summary: Noroviruses are found to be prevalent among hospitalized children in Indonesia, particularly in those under 2 years old. Different predominant genotypes were observed in different years, with GII.4 Sydney_2012 being the most common genotype from 2015 to 2018. The study highlights the significance of continuous molecular surveillance of emerging norovirus strains.
INFECTION GENETICS AND EVOLUTION
(2021)
Article
Infectious Diseases
Ngan Thi Kim Pham, Aksara Thongprachum, Yuko Shimizu, Itoe Shiota, Sheikh Ariful Hoque, Pattara Khamrin, Chika Takano, Quang Duy Trinh, Shoko Okitsu, Shihoko Komine-Aizawa, Hiroyuki Shimizu, Niwat Maneekarn, Satoshi Hayakawa, Hiroshi Ushijima
Summary: This study revealed a high genetic diversity of PeV-A circulating in Japanese infants and children with acute gastroenteritis (AGE), with five different genotypes detected including the rare PeV-A2. Clinical symptoms observed in these patients included diarrhea, vomiting, fever, cough, rhinorrhea, and dehydration.
INFECTION GENETICS AND EVOLUTION
(2021)
Review
Virology
Kouichi Kitamura, Hiroyuki Shimizu
Summary: The use of oral poliovirus vaccine (OPV) has been crucial for preventing person-to-person transmission of poliovirus, but challenges such as cVDPV2 and iVDPV infections pose risks for global polio eradication efforts. The identification of chronic type 2 iVDPV (iVDPV2) excretors highlights the potential risk of inapparent iVDPV infection for expanding cVDPV outbreaks. Further research on genetic characterizations and molecular evolution of iVDPV2 will be critical for elucidating the remaining risk of iVDPV2 in the post-OPV era.
Article
Infectious Diseases
Tomonori Nagai, Nozomu Hanaoka, Harutaka Katano, Masami Konagaya, Keiko Tanaka-Taya, Hiroyuki Shimizu, Toshiji Mukai, Tsuguto Fujimoto
Summary: This study presents a rare case of acute encephalopathy caused by coxsackievirus A2 (CV-A2) in a previously healthy female child. The patient's condition rapidly deteriorated within 2 hours, leading to death. This is the first report of a fatal acute encephalitis-dependent on CV-A2 infection in a child.
BMC INFECTIOUS DISEASES
(2021)
Article
Immunology
Shinji Fukushima, Takashi Nakano, Hiroyuki Shimizu, Atsuo Hamada
Summary: This study assessed the catch-up immunization of healthy Japanese adults with standalone conventional inactivated polio vaccine (cIPV). The results showed that catch-up immunization with cIPV induced robust immune responses and was well tolerated in Japanese adults.
Review
Infectious Diseases
Kouichi Kitamura, Hiroyuki Shimizu
Summary: Despite the World Health Organization Western Pacific Region (WPR) maintaining a polio-free status for over two decades, the risk of polio outbreaks associated with circulating vaccine-derived polioviruses (cVDPVs) cannot be ignored. Previous cVDPV outbreaks have been effectively controlled, but the frequency and magnitude of such outbreaks in the WPR have been increasing. Therefore, it is important to reconsider the risks of future polio outbreaks associated with cVDPVs and update immunization and surveillance strategies accordingly.
JAPANESE JOURNAL OF INFECTIOUS DISEASES
(2022)