Article
Biochemistry & Molecular Biology
Olanrewaju Fatola, Markus Keller, Anne Balkema-Buschmann, James Olopade, Martin H. Groschup, Christine Fast
Summary: In this study, the incubation period, lesion profile, and PrPSc profile induced by well-defined sheep scrapie isolates, bovine spongiform encephalopathy (BSE), and ovine BSE were investigated in two lines of ovine PrP overexpressing transgenic mice. The results showed genotype-dependent differences between ARQ and VRQ isolates, and BSE had a longer incubation period in Tgshp XI compared to Tgshp IX. Immunohistochemical analysis revealed distinguishing patterns in certain brain regions for both BSE isolates, differentiating them from scrapie.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mafalda Casanova, Carla Machado, Paula Tavares, Joao Silva, Christine Fast, Anne Balkema-Buschmann, Martin H. Groschup, Leonor Orge
Summary: Portugal was one of the first European countries to report cases of Atypical Scrapie (ASc), and they confirmed through murine bioassays that the ASc strain present in Portuguese sheep corresponds to ASc Nor98.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Juan Carlos Espinosa, Olivier Andreoletti, Alba Marin-Moreno, Severine Lugan, Patricia Aguilar-Calvo, Herve Cassard, Patricia Lorenzo, Jean-Yves Douet, Ana Villa-Diaz, Naima Aron, Irene Prieto, Alvina Huor, Juan Maria Torres
Summary: This study shows that interference with prion propagation in a host expressing two different prion protein genes is related to the transmissibility of the prion in the host expressing only the interfering prion protein. The interference detected occurs in a prion strain-dependent manner and favors the propagation of the murine PrP allele. These findings suggest that host-specific factors may play a role in interfering with prion propagation in addition to the PrP amino acid sequence.
Article
Biochemistry & Molecular Biology
Tomas Barrio, Enric Vidal, Marina Betancor, Alicia Otero, Inmaculada Martin-Burriel, Marta Monzon, Eva Monleon, Marti Pumarola, Juan Jose Badiola, Rosa Bolea
Summary: Neurotrophins and their receptors, including p75(NTR), were studied in the brains of scrapie-affected sheep and infected mice, showing p75(NTR) overexpression in early stages in sheep and higher abundance of p75(NTR) immunostained cells in infected mice. These findings suggest a potential role of p75(NTR) in the neurodegeneration associated with prion diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Juan Carlos Espinosa, Alba Marin-Moreno, Patricia Aguilar-Calvo, Sylvie L. Benestad, Olivier Andreoletti, Juan Maria Torres
Summary: The study showed that only classic BSE can convert pig-PrPC into PrPSc, indicating a low conformational flexibility of pig-PrPC which may confer high resistance to various prion strains. The susceptibility of pigs to different prion strains varies, with only classic BSE showing infectivity.
JOURNAL OF INFECTIOUS DISEASES
(2021)
Review
Biochemistry & Molecular Biology
Monika Olech
Summary: Bovine spongiform encephalopathy (BSE) is a fatal neurodegenerative disease caused by abnormally folded prion protein (PrPSc). It primarily affects cattle, but can also infect sheep and goats. Discriminatory testing is required to differentiate BSE from scrapie, as well as differentiate classical BSE from atypical strains. Various methods have been developed for BSE detection, including identification of characteristic lesions and detection of PrPSc in the brain. This paper aims to summarize these methods, evaluate their diagnostic performance, and highlight their advantages and limitations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Christine Fast, Catherine Graham, Martin Kaatz, Kristina Santiago-Mateo, Tammy Kaatz, Kendra MacPherson, Anne Balkema-Buschmann, Ute Ziegler, Martin H. Groschup, Stefanie Czub
Summary: The study aimed to identify type-specific PrPSc profiles in different forms of BSE using Immunohistochemistry. Brain samples from 21 cattle infected with C-, H-, and L-type BSE were analyzed, along with samples from orally C-type BSE infected animals. The immunohistochemical analysis revealed distinct PrPSc profiles for different BSE types, providing new insights into the pathogenesis of BSE.
Article
Agriculture, Dairy & Animal Science
Laura Garcia-Mendivil, Diego R. Mediano, Adelaida Hernaiz, David Sanz-Rubio, Francisco J. Vazquez, Belen Marin, Oscar Lopez-Perez, Alicia Otero, Juan J. Badiola, Pilar Zaragoza, Laura Ordovas, Rosa Bolea, Inmaculada Martin-Burriel
Summary: This study demonstrates for the first time how ovine mesenchymal stem cells react to scrapie prion infection in vitro and how the differentiation into neuron-like cells increases their susceptibility to prion infection. The results indicate that in neurogenic conditions, ovine MSCs maintain detectable levels of PrPSc and the signal increases progressively over time, while in growth conditions, the PrPSc signal weakens and is lost over time.
Article
Veterinary Sciences
Alba Marin-Moreno, Patricia Aguilar-Calvo, Juan Carlos Espinosa, Maria Zamora-Ceballos, Jose Luis Pitarch, Lorenzo Gonzalez, Natalia Fernandez-Borges, Leonor Orge, Olivier Andreoletti, Romolo Nonno, Juan Maria Torres
Summary: The study on the diversity of goat scrapie strains in Europe revealed the complexity in classifying isolates and differences between scrapie strains in sheep and goats. It also emphasized that classical scrapie in small ruminants is caused by multiple prion strains, not a single strain like epidemic BSE-C.
VETERINARY RESEARCH
(2021)
Article
Agriculture, Dairy & Animal Science
Adelaida Hernaiz, Paula Cobeta, Belen Marin, Francisco Jose Vazquez, Juan Jose Badiola, Pilar Zaragoza, Rosa Bolea, Inmaculada Martin-Burriel
Summary: The study demonstrates for the first time how a three-dimensional culture system improves the susceptibility of ovine mesenchymal stem cells to prion infection, mimicking the toxic effects seen in prion diseases. This model shows potential as an in vitro system for studying prion diseases in a microenvironment similar to in vivo conditions. In neurodegenerative diseases, including prion diseases, cellular in vitro models are important for studying disease mechanisms and potential therapies.
Article
Veterinary Sciences
Marina Betancor, Belen Marin, Alicia Otero, Carlos Hedman, Antonio Romero, Tomas Barrio, Eloisa Sevilla, Jean-Yves Douet, Alvina Huor, Juan Jose Badiola, Olivier Andreoletti, Rosa Bolea
Summary: The transmission of atypical scrapie to cattle can lead to the emergence of BSE-like prion seeding activity, even though the infected cattle did not show clinical signs of prion disease.
VETERINARY RESEARCH
(2023)
Article
Infectious Diseases
Yong-Chan Kim, Sae-Young Won, Min-Ju Jeong, Byung-Hoon Jeong
Summary: This study conducted enhanced surveillance of prion disease-related factors in Korean cattle, did not find the E211K mutation in the PRNP gene, and found significantly different genotype, allele, and haplotype distributions among different cattle breeds. The enhanced surveillance system of BSE was carried out for the first time in Korean cattle.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Veterinary Sciences
Timm Konold, John Spiropoulos, Janet Hills, Hasina Abdul, Saira Cawthraw, Laura Phelan, Amy Mckenna, Lauren Read, Sara Canoyra, Alba Marin-Moreno, Juan Maria Torres
Summary: This study demonstrates for the first time that a TSE agent with BSE-like properties can be amplified in cattle inoculated with atypical scrapie brain homogenate.
VETERINARY RESEARCH
(2023)
Review
Veterinary Sciences
Pablo Alarcon, Francisco Marco-Jimenez, Verity Horigan, Angel Ortiz-Pelaez, Brenda Rajanayagam, Aidan Dryden, Hugh Simmons, Timm Konold, Carmen Marco, Judith Charnley, John Spiropoulos, Claire Cassar, Amie Adkin
Summary: The effectiveness of current cleaning and disinfection protocols for classical scrapie outbreaks on farms remains unclear, with recommendations mainly based on laboratory experiments. Experts suggest that there are no practical, cost-effective alternatives at the moment, therefore control should focus on a combination of cleaning and disinfection, temporal bans on restocking, and replacement with less susceptible livestock.
PREVENTIVE VETERINARY MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
Sae-Young Won, Yong-Chan Kim, Kyoungtag Do, Byung-Hoon Jeong
Summary: Prion disease is a fatal infectious disease caused by the accumulation of PrPSc in several mammals, but has not been reported in horses. This study investigated the genetic characteristics of the equine SPRN gene and found four SNPs with different distributions among three horse breeds. Notably, there were no non-synonymous SNPs in the equine SPRN gene, which are commonly found in prion disease-susceptible animals.
Review
Neurosciences
Anna Bencsik, Philippe Lestaevel, Irina Guseva Canu
PROGRESS IN NEUROBIOLOGY
(2018)
Review
Virology
Sarah Madrieres, Guillaume Castel, Severine Murri, Johann Vulin, Philippe Marianneau, Nathalie Charbonnel
Article
Microbiology
Severine Murri, Sarah Madrieres, Caroline Tatard, Sylvain Piry, Laure Benoit, Anne Loiseau, Julien Pradel, Emmanuelle Artige, Philippe Audiot, Nicolas Lemenager, Sandra Lacote, Johann Vulin, Nathalie Charbonnel, Philippe Marianneau, Guillaume Castel
Article
Microbiology
Sarah Madrieres, Caroline Tatard, Severine Murri, Johann Vulin, Maxime Galan, Sylvain Piry, Coralie Pulido, Anne Loiseau, Emmanuelle Artige, Laure Benoit, Nicolas Lemenager, Latifa Lakhdar, Nathalie Charbonnel, Philippe Marianneau, Guillaume Castel
Article
Biotechnology & Applied Microbiology
Elodie Monchatre-Leroy, Sandrine Lesellier, Marine Wasniewski, Evelyne Picard-Meyer, Celine Richomme, Franck Boue, Sandra Lacote, Severine Murri, Coralie Pulido, Johann Vulin, Francisco J. Salguero, Meriadeg Ar Gouilh, Alexandre Servat, Philippe Marianneau
Summary: This study conducted intranasal SARS-CoV-2 infection experiments in ferrets and hamsters, finding that hamsters were more susceptible to severe illness than ferrets under 103 pfu infection conditions. The viral RNA was detected in the lungs of hamsters but not in ferrets, and in the brain of both species. However, the clinical disease in both species remained mild overall, with the virus becoming undetectable within 14 days post-inoculation.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Microbiology
Johann Vulin, Severine Murri, Sarah Madrieres, Maxime Galan, Caroline Tatard, Sylvain Piry, Gabriele Vaccari, Claudia D'Agostino, Nathalie Charbonnel, Guillaume Castel, Philippe Marianneau
Summary: The study describes the isolation of two strains of PUUMALA orthohantavirus from two distinct French geographical areas: Ardennes (endemic area) and Loiret (non-endemic area). By selecting wild bank voles, cultivating the strains efficiently in Vero E6 cells, and propagating them in immunologically neutral bank voles, the impact of isolation methods on viral diversity was better assessed through high-throughput and Sanger sequencing results.
Article
Environmental Sciences
Quentin Hamdaoui, Yanis Zekri, Sabine Richard, Denise Aubert, Romain Guyot, Suzy Markossian, Karine Gauthier, Francois Gaie-Levrel, Anna Bencsik, Frederic Flamant
Summary: The study revealed that exposure to nanopesticides had an impact on the neurodevelopment of mice, but the neurodevelopmental effects were surprisingly lower with combined exposure compared to each substance separately.
Article
Biochemistry & Molecular Biology
Jean-Noel Arsac, Marianne Sedru, Mireille Dartiguelongue, Johann Vulin, Nathalie Davoust, Thierry Baron, Bertrand Mollereau
Summary: Parkinson's disease is characterized by the accumulation of alpha-Synuclein in neuronal cells, induced by exposure to herbicide Paraquat. This exposure also leads to increased levels of alpha Syn in the brain of fruit flies and synergistically induces mortality. Furthermore, Paraquat causes the accumulation of toxic soluble and misfolded forms of alpha-Synuclein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Toxicology
Luiz W. Ribeiro, Mathea Pietri, Hector Ardila-Osorio, Anne Baudry, Francois Boudet-Devaud, Chloe Bizingre, Zaira E. Arellano-Anaya, Anne-Marie Haeberle, Nicolas Gadot, Sonja Boland, Stephanie Devineau, Yannick Bailly, Odile Kellermann, Anna Bencsik, Benoit Schneider
Summary: This study demonstrates that titanium dioxide and carbon black nanoparticles can bind to the cellular form of the prion protein, causing adverse effects on neurons and contributing to the development of Alzheimer's disease. The study identifies signaling pathways through which the nanoparticles alter redox equilibrium, increase neuronal sensitivity to neuroinflammation, and lead to the production of neurotoxic peptides.
PARTICLE AND FIBRE TOXICOLOGY
(2022)
Review
Toxicology
G. Jean Harry, Sandra McBride, Shannah K. Witchey, Sakina Mhaouty-Kodja, Alain Trembleau, Matthew Bridge, Anna Bencsik
Summary: Neurobehavioral phenotyping and assessment has experienced a resurgence in recent decades, resulting in a robust database on rodent performance and its associations with human disorders and therapeutic interventions. However, the use of behavior to assess environmental neurotoxicity is still questioned. Applying new paradigms and understanding the biological basis of behavior can help minimize variability and maximize information in neurobehavioral testing.
FRONTIERS IN TOXICOLOGY
(2022)
Article
Environmental Sciences
Quentin Hamdaoui, Anna Bencsik, Frederic Flamant, Simon Delcour, Tatiana Mace, Sophie Vaslin-Reimann, Francois Gaie-Levrel
Summary: Agriculture emits a significant quantity of airborne contaminants, including nanopesticides, which raise concerns about inhalation risks. Toxicological models are essential for estimating the health consequences of nanopesticides, and studying the potential synergistic toxicity with traditional pesticides is crucial for health impact assessment.
AEROSOL AND AIR QUALITY RESEARCH
(2021)
Article
Toxicology
Anna Bencsik, Philippe Lestaevel
Summary: This article emphasizes the need to integrate parameters and tools from multiple sources when examining the neurotoxicity of nanomaterials, and it suggests a prioritized list of substances for modern neurotoxicology.
FRONTIERS IN TOXICOLOGY
(2021)
Letter
Urology & Nephrology
Delphine Boudard, Federica Aureli, Blandine Laurent, Nathalie Sturm, Andrea Raggi, Emilie Antier, Latifa Lakhdar, Patrice N. Marche, Michele Cottier, Francesco Cubadda, Anna Bencsik
KIDNEY INTERNATIONAL REPORTS
(2020)
Article
Urology & Nephrology
Delphine Boudard, Federica Aureli, Blandine Laurent, Nathalie Sturm, Andrea Raggi, Emilie Antier, Latifa Lakhdar, Patrice N. Marche, Michele Cottier, Francesco Cubadda, Anna Bencsik
KIDNEY INTERNATIONAL REPORTS
(2019)
Proceedings Paper
Nanoscience & Nanotechnology
S. Manixay, S. Delaby, F. Gaie-Levrel, M. Wiart, C. Motzkus, A. Bencsik
5TH NANOSAFE INTERNATIONAL CONFERENCE ON HEALTH AND SAFETY ISSUES RELATED TO NANOMATERIALS FOR A SOCIALLY RESPONSIBLE APPROACH (NANOSAFE 2016)
(2017)