Article
Parasitology
Juri Kim, Eun-Ah Park, Mee Young Shin, Soon-Jung Park
Summary: This study confirms the transcriptional activation function of GlMyb2 during encystation in Giardia lamblia. Through chromatin immunoprecipitation experiments and quantitative real-time PCR, several target genes of GlMyb2 were identified, with a detailed analysis on the promoter region of glcwp1 gene.
PARASITES & VECTORS
(2022)
Article
Parasitology
Mari Shiratori, Abhishek Patel, Richard W. Gerhold, Steven A. Sullivan, Jane M. Carlton
Summary: Recent studies have shown that Trichomonas vaginalis can establish persistent infections in the vagina, and this understudied infection can have adverse consequences such as increased risk of HIV-1 infection and cervical cancer. Further research on the spherical form of T. vaginalis called 'pseudocyst' and persistent infections is necessary to improve the diagnosis and treatment of trichomoniasis.
TRENDS IN PARASITOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Summary: In this study, it was found that MLF protein is expressed under certain stress conditions and is associated with specific vesicle structures. MLF plays an important role in cell survival under stress conditions, and MLF vesicles share similar stress-induced characteristics with autophagy compartments.
Article
Biochemistry & Molecular Biology
Jui-Hsuan Wu, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Soo-Wah Gan, Jo-Yu Liao, Chao-Cheng Cho, Bo-Chi Lin, Pei-Wei Chiu, Yu-Jiao Pan, Yu-Yun Kao, Yu-Chen Liu, Chin-Hung Sun
Summary: The study revealed that MLF, FYVE, and ATG8L play a positive role in encystation and function in protein clearance pathway, which is important for encystation and coordinated with Exosomes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Biochemistry & Molecular Biology
Yu-Chien Chen, Szu-Yu Tung, Chia-Wei Huang, Soo-Wah Gan, Bo-Chi Lin, Chia-Wei Chen, Zi-Qi Lin, Chin-Hung Sun
Summary: This study investigated the role of the topoisomerase Spo11 in the encystation process of Giardia lamblia. The findings indicate that Spo11 acts as a positive regulator in activating encystation, interacting with transcription factors involved in cwp gene expression for cyst formation. Disruption of the spo11 gene led to decreased cwp gene expression and cyst formation, highlighting the importance of Spo11 in regulating Giardia differentiation into cysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biochemistry & Molecular Biology
Finn E. von Eyben, Karsten Kristiansen, Daniel S. Kapp, Rong Hu, Ovidiu Preda, Francisco F. Nogales
Summary: This review summarizes the literature on the gene expression and epigenetic regulation of germ cell tumors of the testis type II (TGCT). The seminoma subtype of TGCT expresses a panel of induced pluripotent stem cell (iPSC) with four upregulated genes, while the embryonal carcinoma (EC) subtype has a different set of four upregulated genes. The epigenetic mechanisms, such as DNA methylation and histone modifications, regulate the expression of these driver genes and contribute to the clinical characteristics of TGCT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Immunology
Jingxue Wu, Yongwu Yang, Lin Liu, Weining Zhu, Min Liu, Xiran Yu, Wei Li
Summary: Giardia duodenalis is a common parasitic protozoan that infects the upper small intestine and causes giardiasis, a common gastrointestinal disease. This study found that Giardia infection can induce autophagy in intestinal epithelial cells (IECs), and that autophagy is associated with the pathogenesis of giardiasis, including tight junction defects and NO release.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Moon Hee Yang, Timothy H. Tran, Bethany Hunt, Rebecca Agnor, Christian W. Johnson, Bing Shui, Timothy J. Waybright, Jonathan A. Nowak, Andrew G. Stephen, Dhirendra K. Simanshu, Kevin M. Haigis
Summary: An allosteric network involving lysine 104 and residues in the switch-II domain is essential for KRAS oncogenicity, which could be utilized in the development of inhibitors targeting the activated oncoprotein.
Article
Biochemistry & Molecular Biology
Lautaro Natali, Gabriel Luna Pizarro, Sofia Moyano, Benjamin de la Cruz-Thea, Juliana Musso, Andrea S. Ropolo, Norbert Eichner, Gunter Meister, Melina M. Musri, Constanza Feliziani, Maria C. Touz
Summary: The genetically related assemblages of the intestinal protozoa parasite Giardia lamblia are morphologically indistinguishable and are often derived from specific hosts. RNA sequencing analysis revealed that the exosomal-like vesicles (ElVs) of each assemblage contained distinct small RNA (sRNA) biotypes. These sRNAs may play a regulatory role in parasite communication and contribute to host-specificity and pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Keiko Uechi, Hajime Yaguchi, Jikian Tokashiki, Toki Taira, Osamu Mizutani
Summary: This study identified the nigeran synthase gene in Aspergillus luchuensis, revealed genes involved in nigeran synthesis, and showed the roles of agtC and gnsA in regulating nigeran production and polymerization. These findings contribute to a deeper understanding of fungal cell wall biosynthesis and the potential applications of nigeran as a novel biopolymer.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Dou Ye, Fang Du, Qingxiu Hu, Yajie Zou, Xue Bai
Summary: This study compared the primordium expression profiles of Pleurotus eryngii under blue-light stimulation, red-light stimulation, and darkness using high-throughput sequencing. A large number of differentially expressed genes (DEGs) related to light stimulation and amino acid biosynthesis were identified. The MAPK signaling pathway, oxidative phosphorylation pathway, and RNA transport were shown to be most active during primordium differentiation. The blue-light photoreceptor WC-1 and Deoxyribodipyrimidine photolyase PHR were predicted to play important roles in the primordium differentiation of P. eryngii. The results of this study provide a speculative mechanism for light-induced primordium differentiation and a foundation for further research on fruiting body development in P. eryngii.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Miku Fukawa, Remina Shirai, Tomohiro Torii, Kenta Nakata, Shoya Fukatsu, Takanari Sato, Keiichi Homma, Yuki Miyamoto, Junji Yamauchi
Summary: The molecular mechanisms of neuronal process growth are complex and involve the regulation of extracellular and intracellular signals. This study discovered that HSPA5, a heat shock protein, is secreted from neuronal cells and inhibits the elongation of neuronal processes. The inhibition can be neutralized by antibodies, suggesting that HSPA5 acts as a negative regulator of neuronal differentiation. The study also implicates LRP1 as a potential receptor for HSPA5.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Jae-Ryoung Park, Yoon-Hee Jang, Eun-Gyeong Kim, Sang-Sun Hur, Kyung-Min Kim, Deyong Ren
Summary: In this study, QTLs and candidate genes related to rice plant height regulation were identified, which can provide information and foundation for marker-assisted selection and breeding.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Liping Tan, Xuan Liu, Huan Dou, Yayi Hou
Summary: Mesenchymal stem cells (MSCs) have been extensively studied in the field of regenerative medicine and cell therapy due to their self-renewal, multilineage differentiation, and immune regulation capacities. This review summarizes the plasticity of MSCs in different diseases and tissue sources, as well as the microenvironmental factors and molecular mechanisms that regulate their plasticity.
Article
Biochemistry & Molecular Biology
Chin-Hung Sun, Shih-Che Weng, Jui-Hsuan Wu, Szu-Yu Tung, Li-Hsin Su, Meng-Hsuan Lin, Gilbert Aaron Lee
Article
Biochemistry & Molecular Biology
S. -C. Weng, S. -H. Shiao
INSECT MOLECULAR BIOLOGY
(2020)
Article
Multidisciplinary Sciences
Chalida Sri-in, Shih-Che Weng, Shin-Hong Shiao, Wu-Chun Tu
Article
Chemistry, Medicinal
Chao-Ming Hsieh, Chun-Yi Chen, Ji-Wang Chern, Nei-Li Chan
JOURNAL OF MEDICINAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Shao-Wei Huang, Zi-Qi Lin, Szu-Yu Tung, Li-Hsin Su, Chun-Che Ho, Gilbert Aaron Lee, Chin-Hung Sun
Summary: The study revealed that Giardia's MBF1 gene plays a crucial role in cyst differentiation by binding to key promoter regions of cwp1-3 and myb2 genes and interacting with transcription factors to up-regulate cwp gene expression during encystation. This positive regulation of MBF1 in Giardia suggests a functional conservation of the gene in driving cyst formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Shih-Che Weng, Hsing-Han Li, Jian-Chiuan Li, Wei-Liang Liu, Chun-Hong Chen, Shin-Hong Shiao
Summary: The research identified and characterized the role of complement-like protein TEP1 in limiting dengue virus infection in mosquitoes, showing that TEP1 transcription is induced post-infection and silencing TEP1 leads to increased viral replication. Additionally, a transgenic mosquito line with TEP1 loss-of-function showed higher viral protein levels and titers, along with inhibited expression of key transcription factors and antimicrobial peptides. These results suggest TEP1 regulates immune response and controls dengue virus replication in mosquitoes, providing new insights into host factors in regulating DENV replication.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jui-Hsuan Wu, Szu-Yu Tung, Chun-Che Ho, Li-Hsin Su, Soo-Wah Gan, Jo-Yu Liao, Chao-Cheng Cho, Bo-Chi Lin, Pei-Wei Chiu, Yu-Jiao Pan, Yu-Yun Kao, Yu-Chen Liu, Chin-Hung Sun
Summary: The study revealed that MLF, FYVE, and ATG8L play a positive role in encystation and function in protein clearance pathway, which is important for encystation and coordinated with Exosomes.
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2021)
Article
Parasitology
Shih-Che Weng, Po-Nien Tsao, Shin-Hong Shiao
Summary: This study found that blood glucose enhances DENV replication in mosquitoes, leading to increased virus transmission. The activation of AKT and TOR signaling pathways plays a crucial role in facilitating this process. This insight may contribute to understanding the relationship between dengue fever and diabetes mellitus and could potentially offer new targets for antiviral therapies.
PARASITES & VECTORS
(2021)
Article
Chemistry, Physical
Haoyu Tang, Yijie Tang, Igor Kurnikov, Hsuan-Jen Liao, Nei-Li Chan, Maria G. Kurnikova, Yisong Guo, Wei-chen Chang
Summary: This study successfully bridged the gap between biological and synthetic strategies by combining chemical synthesis and enzymatic synthesis, leading to the preparation of quinolone alkaloid analogues, demonstrating effective chemoenzymatic cascades.
Article
Biochemistry & Molecular Biology
Yu-Chien Chen, Szu-Yu Tung, Chia-Wei Huang, Soo-Wah Gan, Bo-Chi Lin, Chia-Wei Chen, Zi-Qi Lin, Chin-Hung Sun
Summary: This study investigated the role of the topoisomerase Spo11 in the encystation process of Giardia lamblia. The findings indicate that Spo11 acts as a positive regulator in activating encystation, interacting with transcription factors involved in cwp gene expression for cyst formation. Disruption of the spo11 gene led to decreased cwp gene expression and cyst formation, highlighting the importance of Spo11 in regulating Giardia differentiation into cysts.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Shih-Che Weng, Yu-Xian Zhou, Shin-Hong Shiao
Summary: This study developed a novel antiviral approach using a virus-inducible gene expression system to block the replication and transmission of dengue virus and Zika virus. The system successfully inhibited viral replication and production of infectious virus particles both in vitro and in mosquitoes.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Microbiology
Shih-Che Weng, Shin-Hong Shiao
Summary: This study investigated the impact of protein SUMOylation on dengue virus replication in A. aegypti. The findings suggest that protein SUMOylation plays an important role in dengue virus replication and transmission.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biology
Jui-Hsuan Wu, Jen-Chi Lee, Chun-Che Ho, Pei-Wei Chiu, Chin-Hung Sun
Summary: In this study, it was found that MLF protein is expressed under certain stress conditions and is associated with specific vesicle structures. MLF plays an important role in cell survival under stress conditions, and MLF vesicles share similar stress-induced characteristics with autophagy compartments.
Article
Biochemistry & Molecular Biology
Chun -Ting Yeh, Shih-Che Weng, Po-Nien Tsao, Shin -Hong Shiao
Summary: Binding immunoglobulin protein (BiP) plays a crucial role in both dengue virus replication and mosquito reproduction. Silencing BiP expression inhibits viral replication and reduces mosquito fertility and fecundity.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)