Article
Multidisciplinary Sciences
Chayapol Tungphatthong, Santhosh Kumar J. Urumarudappa, Supita Awachai, Thongchai Sooksawate, Suchada Sukrong
Summary: This study reveals that DNA barcoding coupled with HRM is an efficient tool with which to identify M. speciosa and M. speciosa-containing samples and ensure the safety and quality of traditional Thai herbal medicines.
SCIENTIFIC REPORTS
(2021)
Article
Food Science & Technology
Kaan Hurkan, Menekse Bulut
Summary: A DNA-based high resolution melting (HRM) assay was developed to accurately identify the animal species sources in milk and detect cross-adulteration. This method showed high specificity based on nucleotide variations and could detect adulteration down to 0.5% and 1% for certain species. It provides a rapid, sensitive, and cost-effective tool for milk authentication.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2023)
Article
Computer Science, Interdisciplinary Applications
Fatma Ozge Ozkok, Mete Celik
Summary: The study proposed a method for classifying HRM data using HRM images generated from recurrence plots. By inputting black-white recurrence plot representations of HRM data into CNN models, high classification accuracy for species identification was achieved.
COMPUTER METHODS AND PROGRAMS IN BIOMEDICINE
(2021)
Article
Microbiology
Fabien Vorimore, Rachid Aaziz, Bertille de Barbeyrac, Olivia Peuchant, Monika Szymanska-Czerwinska, Bjorn Herrmann, Christiane Schnee, Karine Laroucau
Summary: C. psittaci is the causative agent of avian chlamydiosis and human psittacosis. A PCR-HRM assay was developed to screen for phylogenetically informative SNPs related to the identified C. psittaci lineages, allowing quick identification of the source of infection. The method was validated with reference strains and field isolates, and was consistent with previous genotyping data.
Article
Entomology
Lilian de Oliveira Guimaraes, Roseli Franca Simoes, Carolina Romeiro Fernandes Chagas, Regiane Maria Tironi de Menezes, Fabiana Santos Silva, Eliana Ferreira Monteiro, Marcia Moreira Holcman, Miklos Maximiliano Bajay, Adriano Pinter, Vera Lucia Fonseca de Camargo-Neves, Karin Kirchgatter
Summary: This study analyzed the potential transmission of parasites carried by mosquitoes in zoological gardens in forest areas, identifying new potential vectors and expanding the knowledge on the transmission of avian malaria parasites. This contributes to the understanding of bird conservation and mosquito control measures.
Article
Microbiology
Juliana de Jesus Guimaraes Ferreira, Sandra Cecilia Botelho Costa, Marcelo Addas-Carvalho, Mariane Barroso Pereira, Adriana de Oliveira Franca, Rodrigo Goncalves de Lima, Paula Durante Andrade, Jamiro da Silva Wanderley, Luiz Claudio Martins, Eros Antonio de Almeida, Glaucia Elisete Barbosa Marcon
Summary: Blood donation in Brazil is regulated by the Brazilian Ministry of Health, and the screening for Chagas disease and leishmaniasis is not well established. This study used molecular techniques to clarify cases of blood donation candidates with inconclusive serology for Chagas disease and to analyze the difference in melting temperature during real-time PCR. The results highlight the importance of using molecular tests for confirmation in the screening process at blood banks.
Article
Microbiology
Jaber Ghorbani, Farhad Bonakdar Hashemi, Fereshteh Jabalameli, Mohammad Emaneini, Reza Beigverdi
Summary: The study demonstrates that the MHRM assay can successfully identify five respiratory pathogens in less than 5 hours, with 100% sensitivity and 88.8-100% specificity.
Article
Parasitology
Glaucilene da Silva Costa, Antonio Marques Pereira Junior, Thais Santos Castro, Paula Frassinetti Medeiros de Paulo, Gabriel Eduardo Melim Ferreira, Jansen Fernandes Medeiros
Summary: Cutaneous Leishmaniasis (CL) is a disease caused by Leishmania species and transmitted by sand flies. This study evaluated the sand fly fauna in rural areas of Rondonia State, identifying various species but not finding Leishmania DNA. The feeding behavior of field-caught sand flies was observed, providing insight into local vectors and potential reservoirs for Leishmania spp.
Article
Biochemistry & Molecular Biology
Ludmila Khrustaleva, Mais Nzeha, Aleksey Ermolaev, Ekaterina Nikitina, Valery Romanov
Summary: High-resolution melting (HRM) analysis is an effective method for quick post-PCR analysis. A two-step HRM marker system was developed to identify the N-, S-, R- and T-cytoplasms of onions. Through testing 246 onion plants, the HRM analysis showed that S-cytoplasm is the most common source of CMS, while R-cytoplasm is the rarest type, and the proportion of T-cytoplasm among the analyzed breeding lines was 20.5%. Compared to traditional phenotype identification, HRM system enables rapid and accurate discrimination of the four types of onion cytoplasm.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Mario Rene Moya-Moraga, Cesar Perez-Ruiz
Summary: This study investigated the distribution and genetic diversity of the Chilean hazelnut, and used the MaxEnt model and High-Resolution Melting Analysis (HRM) to predict its response to climate change and anthropic activities. The results showed that temperature and water vapor were key factors for the species' distribution. The future prediction model indicated a decrease in habitat range, and identified priority areas for protection based on genetic diversity and allelic richness.
Article
Food Science & Technology
Jane Kagure Njaramba, Lillian Wambua, Titus Mukiama, Nelson Onzere Amugune, Jandouwe Villinger
Summary: Substituting high commercial-value meats with cheaper or undesirable species is a common form of food fraud, raising ethical and dietary concerns. PCR-HRM analysis targeting mitochondrial genes is a cost-effective technique for detecting species substitution in meat samples. This approach has broad applications in protecting consumers against food fraud in low- and middle-income countries as well as developed ones.
Article
Entomology
Jose Alejandro Martinez-Ibarra, Benjamin Nogueda-Torres, Oziel Dante Montanez-Valdez, J. Guadalupe Michel-Parra
Summary: This study evaluated the influence of different hosts on the biological parameters of Triatoma pallidipennis. The results showed significant differences in development period, survival probability, and reproductive capacity among different hosts. These differences may be related to the nutritional value of the host's blood and the energy expenditure required for digestion.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Parasitology
Miguel Saavedra, Antonella Bacigalupo, Maria Victoria Barrera, Maria J. Vergara, Barbara Alvarez-Duhart, Catalina Munoz-San Martin, Rigoberto Solis, Pedro E. Cattan
Summary: Mepraia spinolai, a vector of Trypanosoma cruzi in Chile, was found to have a relationship between its parasitic load of T. cruzi and its blood meal source and infecting DTUs.
Article
Food Science & Technology
Xiaoye Chen, Mingyang Fu, Wenjie Xu, Manhong Huang, Xiaowen Cui, Min Cao, Xiaohui Xiong, Xiong Xiong
Summary: The study successfully developed a novel one-step triplex high-resolution melting (HRM) analysis for rapid authentication of commonly found Gadiformes species on the Chinese market. By targeting mitochondrial genes, this technique showed high specificity and discrimination capabilities. Validation on commercial products demonstrated the suitability of this method for rapid identification of Gadiformes species.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Parasitology
Yokomi N. Lozano-Sardaneta, Eduardo Jimenez-Giron, Jorge J. Rodriguez-Rojas, Sokani Sanchez-Montes, Lucia Alvarez-Castillo, Victor Sanchez-Cordero, Ingeborg Becker
Summary: This study evaluated the species diversity of sand flies and identified their blood meal sources in the Biosphere Reserve Los Tuxtlas, Veracruz. Psathyromyia aclydifera and Psychodopygus panamensis were found to be the most abundant species, with human beings being the most common source of the blood meal. This study provides important information about the interactions between sand flies, hosts, and Leishmania species.
Article
Biochemistry & Molecular Biology
Tassiana Gutierrez de Paula, Bruna Tereza Thomazini Zanella, Leonardo Nazario de Moraes, Jessica Silvino Valente, Jordana Inacio Nascimento-Oliveira, Edson Assuncao Mareco, Geysson Javier Fernandez, Maeli Dal-Pai-Silva
Summary: The study analyzed the sharing of piRNA clusters among three genotypes and identified 24 piRNA clusters that are common among them. Gene expression analysis revealed differences in the expression of certain piRNAs among the genotypes.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR & INTEGRATIVE PHYSIOLOGY
(2022)
Article
Cell Biology
Juan Felipe Valdes-Lopez, Geysson J. Fernandez, Silvio Urcuqui-Inchima
Summary: The study reveals that CHIKV infection induces high levels of IL27 in MDMs, promoting pro-inflammatory and antiviral response through activation of TLR1/2-MyD88 and TLR3-TRIF signaling pathways, independent of IFN signaling.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Microbiology
Lidia Gual-Gonzalez, Catalina Arango-Ferreira, Laura Camila Lopera-Restrepo, Omar Cantillo-Barraza, Daniela Velasquez Marin, Natalia Restrepo Bustamante, Omar Triana-Chavez, Melissa S. Nolan
Summary: Chagas disease, caused by Trypanosoma cruzi infection, is a major cause of heart failure in Latin America. Early diagnosis and treatment are crucial to prevent irreversible myocardial damage. However, in Colombia, less than 1% of cases are treated due to various barriers. This study presents two cases of pediatric oral transmission of Chagas disease in an area of Colombia where this mode of transmission was previously unreported.
Article
Multidisciplinary Sciences
Arley Calle-Tobon, Juliana Perez-Perez, Nicolas Forero-Pineda, Omar Triana Chavez, Winston Rojas-Montoya, Guillermo Rua-Uribe, Andres Gomez-Palacio
Summary: This study examined the virome structure, presence of Wolbachia, and mitochondrial diversity in field-caught Ae. aegypti and Ae. albopictus mosquitoes in Medellin, Colombia. The virome composition differed significantly between the two species, with Ae. albopictus harboring a greater number of insect-specific viruses (ISVs). Wolbachia was present in both species, but its presence did not correlate with changes in the mosquito virome. The mitochondrial diversity of Ae. aegypti indicated a change in the population starting in the second half of 2017, coinciding with the release of Wolbachia-infected mosquitoes.
Article
Entomology
Andrea Arevalo-Cortes, Yurany Granada, David Torres, Omar Triana-Chavez
Summary: This study analyzed the characteristics of Aedes aegypti populations from different cities in Colombia, including lifespan, fecundity, and mortality. The study found that each population had unique features and patterns, which are important for determining the timing of insecticide application and surveillance.
Article
Biochemistry & Molecular Biology
Mariana F. Fragoso, Geysson J. Fernandez, Lisa Vanderveer, Harry S. Cooper, Michael Slifker, Margie L. Clapper
Summary: This study explored the miR expression profile of precancerous dysplastic lesions in the AOM/DSS mouse model, identifying early molecular changes associated with colitis-associated colorectal cancer (CAC). A miR signature was discovered that can distinguish inflamed non-neoplastic mucosa from dysplasia, providing novel insight into the development of CAC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Geysson Javier Fernandez, Julieta M. Ramirez-Mejia, Silvio Urcuqui-Inchima
Summary: Vitamin D enhances immune response by regulating the expression of microRNAs and mRNAs in macrophage differentiation. Differentially expressed genes are associated with pathophysiological processes such as inflammatory responses and cellular stress. MiRNAs, including miR-1972, miR-1273h-5p, and miR-665, regulate genes related to the inflammatory response.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Virology
Andrea Arevalo-Cortes, Ashish Damania, Yurany Granada, Sara Zuluaga, Rojelio Mejia, Omar Triana-Chavez
Summary: This study identified high Zika virus infection rates in Ae. aegypti females from Colombian rural municipalities with deficient water supply. Differences in diversity were observed between infective and non-infective forms in the presence of natural Zika infection. Bacteria associated with Zika virus infection and lambda-cyhalothrin resistance were detected, suggesting a potential role in virus entry and insecticide bioremediation.
Article
Entomology
Andres Gomez-Palacio, Sebastian Pita, Fernando Abad-Franch, Yoman Monsalve, Omar Cantillo-Barraza, Fernando A. Monteiro, Marcio G. Pavan, Walter S. Santos, Alejandra Panzera, Gabriela Burgueno-Rodriguez, Francisco Panzera
Summary: Through genetic and cytogenetic studies, interpopulation genetic diversity and phylogenetic affinities of T. maculata were investigated. The findings suggest that T. maculata may consist of a complex of at least three sibling taxa.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Polymer Science
Nicolas Montoya-Escobar, Daniel Ospina-Acero, Jorge Andres Velasquez-Cock, Catalina Gomez-Hoyos, Angelica Serpa Guerra, Piedad Felisinda Ganan Rojo, Lina Maria Velez Acosta, Juan Pablo Escobar, Natalia Correa-Hincapie, Omar Triana-Chavez, Robin Zuluaga Gallego, Pablo M. Stefani
Summary: In this study, the crystallinity of three different types of cellulose was investigated using FTIR and XRD methods. A deconvolution method was developed to accurately estimate the crystallinity index. The effect of milling time on crystallinity was observed.
Article
Entomology
Lidia Gual-Gonzalez, Manuel Medina, Cesar Valverde-Castro, Virgilio Beltran, Rodrigo Caro, Omar Triana-Chavez, Melissa S. Nolan, Omar Cantillo-Barraza
Summary: This study evaluated the feasibility and effectiveness of micro-encapsulated insecticide paints in controlling vector species in a rural community in Colombia. The results showed the potential of this approach to prevent colonization and reduce the risk of disease transmission. The complementary use of insecticide spraying and micro-encapsulated paints could enhance public health efforts and reduce costs.
Article
Biochemistry & Molecular Biology
Driele B. Santos, Geysson J. J. Fernandez, Luciana M. C. Pardini, Maria Ines M. C. Pardini, Adriana C. C. Ferrasi
Summary: Studying the transcriptome of canine mammary ductal carcinoma can help identify genes and pathways related to human breast cancer, leading to the discovery of biomarkers and therapeutic targets. This study identified 633 downregulated and 573 upregulated genes in carcinoma tissue compared to normal tissue, indicating deregulation in inflammatory, cell differentiation and adhesion, and extracellular matrix maintenance pathways. The differentially expressed genes may indicate disease aggressiveness and prognosis. The canine transcriptome serves as an excellent model for oncology research in both humans and animals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Victor Hugo Pena-Garcia, Jeffrey C. Luvall, Rebecca C. Christofferson
Summary: Weather variables, especially temperature, play a significant role in the transmission of vector-borne arboviruses. This study examines the relationship between micro-environmental and macro-level temperature measures in three Colombian cities using field-collected data. The results suggest that weather station data may not accurately represent indoor temperature profiles.
Article
Biochemistry & Molecular Biology
Diogo de Moraes, Felippe Mousovich-Neto, Sarah Santiloni Cury, Jakeline Oliveira, Jeferson dos Santos Souza, Paula Paccielli Freire, Maeli Dal-Pai-Silva, Marcelo Alves da Silva Mori, Geysson Javier Fernandez, Robson Francisco Carvalho
Summary: Aging causes changes in body composition, including an increase in visceral fat mass, which is associated with age-related diseases. Visceral fat can affect systemic metabolism by secreting molecules that act in distal tissues, particularly the liver. However, little is known about age-related changes in visceral fat in humans.
Article
Virology
Victor Hugo Pena-Garcia, Francis M. Mutuku, Bryson A. Ndenga, Joel Omari Mbakaya, Samwuel Otieno Ndire, Gladys Adhiambo Agola, Paul S. Mutuku, Said L. Malumbo, Charles M. Ng'ang'a, Jason R. Andrews, Erin A. Mordecai, A. Desiree LaBeaud
Summary: Most vector control activities in urban areas focus on household environments. However, there is limited information about infection risks in spaces other than households, and the relative risk of these spaces is not fully understood. Using simulations based on data, this study investigated the importance of household and non-household environments for dengue entomological risk in two Kenyan cities. The results suggest that non-household environments have a higher density of vectors and pose a potential risk for dengue transmission.
