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Multidisciplinary Sciences
Hui Ma, Tao Li, Xuefeng Xie, Long Jiang, Jingwei Ye, Chenjia Gong, Hanwei Jiang, Suixing Fan, Huan Zhang, Baolu Shi, Beibei Zhang, Xiaohua Jiang, Yang Li, Jianteng Zhou, Jianze Xu, Xingxia Zhang, Xiaoning Hou, Hao Yin, Yuanwei Zhang, Qinghua Shi
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Plant Sciences
Jiayi Zhao, Xin Gui, Ziming Ren, Huiqi Fu, Chao Yang, Wenyi Wang, Qingpei Liu, Min Zhang, Chong Wang, Arp Schnittger, Bing Liu
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Cell Biology
Suixing Fan, Yuewen Wang, Hanwei Jiang, Xiaohua Jiang, Jianteng Zhou, Yuying Jiao, Jingwei Ye, Zishuo Xu, Yue Wang, Xuefeng Xie, Huan Zhang, Yang Li, Wei Liu, Xiangjun Zhang, Hui Ma, Baolu Shi, Yuanwei Zhang, Muhammad Zubair, Wasim Shah, Zhipeng Xu, Bo Xu, Qinghua Shi
Summary: This study reports the discovery of a novel recombination protein, C12ORF40/REDIC1, which plays a crucial role in meiotic crossover formation in male mammals. The absence of REDIC1 results in meiotic arrest and insufficient crossover formation, as observed in both mice and patients. Biochemical analysis reveals that REDIC1 interacts with recombination intermediates and this interaction is impaired by the identified mutation. These findings provide potential molecular targets for the clinical diagnosis and therapy of infertility.
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Plant Sciences
Xiang Li, Mingsen Yu, Pablo Bolanos-Villegas, Jun Zhang, Di'an Ni, Hong Ma, Yingxiang Wang
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Plant Sciences
Ayako Nishizawa-Yokoi, Ritsuko Motoyama, Tsuyoshi Tanaka, Akiko Mori, Keiko Iida, Seiichi Toki
Summary: Land plants have developed defense systems, including DNA damage response (DDR) and DNA repair systems, to protect themselves. In rice, the transcription factor OsSOG1 plays a crucial role in controlling DDR and DNA repair and has differences compared to Arabidopsis.
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Biochemistry & Molecular Biology
Xiaofan Jin, Geoff Fudenberg, Katherine S. Pollard
Summary: Active, spatially accessible genomic regions during meiotic prophase are associated with DSB-favored loci, which further adopt a transient locally active configuration in early prophase. Conversely, crossover formation is depleted among DSBs in spatially accessible regions during meiotic prophase, particularly within gene bodies. Active chromatin regions have smaller average loop sizes in mammalian meiosis, indicating differences in chromatin architecture along chromosomal axes are associated with variable recombination activity.
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Biochemistry & Molecular Biology
Erik Toraason, Anna Horacek, Cordell Clark, Marissa L. Glover, Victoria L. Adler, Tolkappiyan Premkumar, Alina Salagean, Francesca Cole, Diana E. Libuda
Summary: During meiosis, sister chromatids or intra-chromatids can serve as repair templates for double-strand DNA breaks (DSBs), with noncrossover recombination being the predominant outcome. The XPF-1 nuclease likely functions in late prophase to promote repair using sister chromatids or intra-chromatids.
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Biotechnology & Applied Microbiology
Megumi Ishii, Tetsuya Ishii
Summary: This passage discusses whether genome-edited agricultural products should be considered genetically modified organisms (GMOs) and how to prove definitively that a genome-edited organism does not contain exogenous DNA, taking social aspects into account.
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Multidisciplinary Sciences
Jakub Wiktor, Arvid H. Gynna, Prune Leroy, Jimmy Larsson, Giovanna Coceano, Ilaria Testa, Johan Elf
Summary: Homologous recombination is crucial for accurate DNA repair, with repair of double-stranded DNA breaks in E. coli completed in approximately 15 minutes. The search for homologous repair templates takes less than 9 minutes and is mediated by a thin and highly dynamic RecA filament stretching throughout the cell. This model effectively reduces search dimensionality, with search time consistent across different cell lengths and DNA amounts.
Review
Cell Biology
Vikash Kumar Yadav, Corentin Claeys Bouuaert
Summary: The article outlines the latest advances in understanding the mechanism of meiotic DSB formation, emphasizing the tight regulation of DSBs and the significance of recombination mechanisms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
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Biochemistry & Molecular Biology
Jaroslav Fulnecek, Eva Klimentova, Albert Cairo, Sona Valuchova Bukovcakova, Panagiotis Alexiou, Zbynek Prokop, Karel Riha
Summary: The C-terminal SAP domain of Ku70 is involved in the initial phases of Ku-DNA interaction but not the sliding process. Ku lacking the SAP domain can still function in classical NHEJ and telomere maintenance.
NUCLEIC ACIDS RESEARCH
(2023)
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Biochemistry & Molecular Biology
Yunfei Li, Yumin Huang, Huayue Sun, Tianyi Wang, Wei Ru, Lingling Pan, Xiaoming Zhao, Zhaobin Dong, Wei Huang, Weiwei Jin
Summary: In maize, HSP101 positively mediates thermotolerance during microsporogenesis. HSP101, encoded by Ms42, plays a role in pairing and synapsis, DNA repair, among other processes in male meiocytes.
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Genetics & Heredity
Fabien Dutreux, Abhishek Dutta, Emilien Peltier, Sabrina Bibi-Triki, Anne Friedrich, Bertrand Llorente, Joseph Schacherer
Summary: In this study, the non-model budding yeast Lachancea waltii was used to investigate meiotic recombination. It was found that L. waltii frequently lacks crossover, suggesting the existence of an alternative mechanism for gamete formation. The interference between meiotic crossovers in L. waltii was reduced compared to S. cerevisiae, and crossover hotspots were not conserved between Lachancea and Saccharomyces clades. This study demonstrates that meiotic recombination can evolve at a limited evolutionary scale within budding yeasts.
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Biology
Marella D. Canny, Michael P. Latham
Summary: The Mre11-Rad50-Nbs1 protein complex plays a crucial role in DNA double-strand break repair. This study reveals the existence of multiple conformations of the complex and investigates their functional implications using nuclease activity assays. The findings provide new insights into the mechanism of MR complex in DNA repair.
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Cell Biology
Juliette Dabin, Margherita Mori, Sophie E. Polo
Summary: This review summarizes recent advances in understanding the coordination between chromatin maintenance and the DNA damage response (DDR) in the cell nucleus. The authors discuss how the DDR affects chromatin marks, organization, and mobility, and how chromatin alterations contribute to the DDR. They also present current knowledge of the molecular mechanisms underlying these processes in physiological and pathological conditions, and highlight unanswered questions in this field.
CURRENT OPINION IN CELL BIOLOGY
(2023)
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Multidisciplinary Sciences
Joiselle Blanche Fernandes, Mathilde Seguela-Arnaud, Cecile Larcheveque, Andrew H. Lloyd, Raphael Mercier
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
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Multidisciplinary Sciences
Heidi Serra, Christophe Lambing, Catherine H. Griffin, Stephanie D. Topp, Divyashree C. Nageswaran, Charles J. Underwood, Piotr A. Ziolkowski, Mathilde Seguela-Arnaud, Joiselle B. Fernandes, Raphael Mercier, Ian R. Henderson
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
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Biotechnology & Applied Microbiology
Chun Wang, Qing Liu, Yi Shen, Yufeng Hua, Junjie Wang, Jianrong Lin, Mingguo Wu, Tingting Sun, Zhukuan Cheng, Raphael Mercier, Kejian Wang
NATURE BIOTECHNOLOGY
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Developmental Biology
Adam Barrada, Meriem Djendli, Thierry Desnos, Raphael Mercier, Christophe Robaglia, Marie-Heprimelene Montane, Benoit Menand
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Genetics & Heredity
Vanessa Tsui, Wayne Crismani
TRENDS IN GENETICS
(2019)
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Biochemistry & Molecular Biology
Chao Yang, Kostika Sofroni, Erik Wijnker, Yuki Hamamural, Lena Carstens, Hirofumi Harashima, Sara Christina Stolze, Daniel Vezon, Liudmila Chelysheva, Zsuzsanna Orban-Nemeth, Gaetan Pochon, Hirofumi Nakagami, Peter Schloegelhofer, Mathilde Grelon, Arp Schnittger
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Multidisciplinary Sciences
Winnie Tan, Vincent J. Murphy, Aude Charron, Sylvie van Twest, Michael Sharp, Angelos Constantinou, Michael W. Parker, Wayne Crismani, Rohan Bythell-Douglas, Andrew J. Deans
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Cell Biology
Wayne Crismani, Raphael Mercier
DEVELOPMENTAL CELL
(2020)
Review
Biochemistry & Molecular Biology
Arun Mouli Kolinjivadi, Wayne Crismani, Joanne Ngeow
HUMAN MOLECULAR GENETICS
(2020)
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Multidisciplinary Sciences
Michael F. Sharp, Vince J. Murphy, Sylvie Van Twest, Winnie Tan, Jennii Lui, Kaylene J. Simpson, Andrew J. Deans, Wayne Crismani
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Biology
Winnie Tan, Sylvie van Twest, Andrew Leis, Rohan Bythell-Douglas, Vincent J. Murphy, Michael Sharp, Michael W. Parker, Wayne Crismani, Andrew J. Deans
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Biochemistry & Molecular Biology
Michael F. Sharp, Rohan Bythell-Douglas, Andrew J. Deans, Wayne Crismani
Summary: DNA-damaging agents can cure some cancers, but with severe side effects. Inhibition of the DNA repair pathway offers a less toxic and more targeted alternative to chemotherapy. The FA core complex is identified as a compelling drug target for cancer therapy, specifically killing certain types of cancer cells.
Editorial Material
Multidisciplinary Sciences
Wayne Crismani, Chloe Girard, Andrew Lloyd
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
Antoine Nicolas, Aude Maugarny-Cales, Bernard Adroher, Liudmila Chelysheva, Yu Li, Jasmine Burguet, Anne-Maarit Bagman, Margot E. Smit, Siobhan M. Brady, Yunhai Li, Patrick Laufs
Summary: Stem cells play crucial roles in animal and plant biology. Two transcription factors are discovered to repress the expression of CUC gene, thus ensuring proper meristem establishment and de novo stem cell niche formation in Arabidopsis thaliana.
Review
Biotechnology & Applied Microbiology
Ruqian Lyu, Vanessa Tsui, Davis J. McCarthy, Wayne Crismani
Summary: Genetic maps are essential for understanding disease genetics and evolutionary processes. Single-cell gamete sequencing has the potential to combine the advantages of short-read sequencing with the ability to build personalized genetic maps, opening up a new space in personalized genetics.