Article
Multidisciplinary Sciences
Scott Curran, Gautam Dey, Paul Rees, Paul Nurse
Summary: This study provides a comprehensive analysis of the spatial and temporal dynamics of cell cycle regulators in the fission yeast Schizosaccharomyces pombe. It reveals that the mitotic B-type cyclin Cdc13 and the regulatory phosphatase Cdc25 exhibit concentration changes at the whole-cell level, with higher concentrations in the nucleus. Additionally, the major fission yeast cyclin-dependent kinase Cdc2, the CDK regulator Sucl, and the inhibitory kinase Weel also show increased nuclear accumulation. The study proposes a two-step process for the control of mitosis.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Oncology
Danxian Jiang, Yin Li, Jinxin Cao, Lianghe Sheng, Xinhai Zhu, Meng Xu
Summary: The CDCA gene family is upregulated in nasopharyngeal carcinoma (NPC) and its expression is associated with adverse prognosis. High expression of CDCA8 is not only associated with poor prognosis, but also with decreased immune infiltration and downregulation of immune checkpoint blockade-related genes in NPC.
FRONTIERS IN ONCOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Arun T. John Peter, Sabine N. S. van Schie, Ngaam J. Cheung, Agnes H. Michel, Matthias Peter, Benoit Kornmann
Summary: The membrane lipid composition of eukaryotic cells varies among different organelles. This study focuses on simplifying and reorganizing yeast phospholipid synthesis by redirecting synthesis reactions to specific subcellular locations. Through genetic screening, genes involved in lipid homeostasis, metabolism, and transport were identified, with a particular focus on the requirement for Csf1 in interorganelle lipid transport and homeostatic adaptation.
Article
Biochemical Research Methods
Chen Jia, Abhyudai Singh, Ramon Grima
Summary: This study investigates the dynamics of cell size in fission yeast, revealing the relationship between different cell growth phases and size control strategies, as well as their effects under various growth conditions. By matching experimental data with theoretical models, the size control strategy and model parameters of cell size distribution are determined.
PLOS COMPUTATIONAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Lei Xu, Chong Zhang, Hang Yin, Shuai Gong, Nai Wu, Zeqiang Ren, Yi Zhang
Summary: RNA modifications play crucial roles in regulating RNA stability and translation, as well as in the mediation of cell death. Cell death is a critical process maintaining tissue homeostasis and is regulated by multiple pathways in response to specific stimuli. This review summarizes the current understanding of the roles of RNA modifications in cell death mediation and discusses future research prospects.
Review
Biochemistry & Molecular Biology
Alisha Shaikh, Austin A. Wesner, Mohanad Abuhattab, Raman G. Kutty, Priyatha Premnath
Summary: Cell cycle regulators have been found to prevent cancerogenesis by acting as inhibitors or activators, and they also play active roles in differentiation, apoptosis, senescence, and other cellular processes. Recent evidence suggests that they are involved in bone healing/development cascade. Our study showed that deletion of p21, a cell cycle regulator, at the G1/S transition, enhanced bone repair capacity after a burr-hole injury in mice. Similarly, another study demonstrated that inhibition of p27 can increase bone mineral density and bone formation. In this review, we provide a concise overview of cell cycle regulators in osteoblasts, osteoclasts, and chondrocytes during bone development and/or healing. Understanding the regulatory processes of cell cycle during bone healing and development is crucial for the development of novel therapies to improve bone healing in aged or osteoporotic fractures.
CELL AND BIOSCIENCE
(2023)
Article
Biology
Nora Sauter, Matteo Sangermani, Isabelle Hug, Urs Jenal, Thomas Pfohl
Summary: A bacteria-on-a-bead system was developed to study the dynamics of flagellum and pili during cell separation and surface attachment. It was found that pili play a crucial role in cell attachment and cooperate with flagellum during the approaching and attaching process to surfaces.
COMMUNICATIONS BIOLOGY
(2022)
Article
Geriatrics & Gerontology
Yu Sun, Ruofan Yu, Hao-Bo Guo, Hong Qin, Weiwei Dang
Summary: The study revealed similar proteomic features between aged cells and cells undergoing caloric restriction, including induction of stress response pathways, suggesting that caloric restriction pathways are activated in aged cells. Additionally, the abnormal changes in mitochondria membrane proteins and proteolytic cellular state in old cells have also drawn attention.These proteomic analyses help identify potential genes and pathways that have causal effects on longevity.
Article
Multidisciplinary Sciences
Masahito Tanaka, Toshiko Kitanishi-Yumura, Shigehiko Yumura
Summary: This study accurately measured the total cell surface area of Dictyostelium cells throughout the cell cycle, showing an exponential increase in surface area during interphase, with cell size regulation primarily governed by the adder or near-adder model. This dynamic adder model suggests that cell membrane turnover plays a key role in maintaining cell size homeostasis during interphase.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Setsu Kato, Kenta Suzuki, Taiki Kenjo, Junya Kato, Yoshiteru Aoi, Yutaka Nakashimada
Summary: Saccharomyces cerevisiae is widely used as a model organism for aging and longevity studies. In a clonal population, cell death in the stationary phase is asynchronous, highlighting heterogeneity in survival at a single-cell level. Cell size heterogeneity and its correlation with death rate are observed, with cells shrinking upon death and a constant proportion between cell size and shrinkage noted in different strains. Further investigation is needed to understand the mechanisms behind this shrinkage event and its implications for cell death and survival heterogeneity.
Article
Multidisciplinary Sciences
Anantha Peramuna, Carmen Quinonero Lopez, Francisco Javier Astorga Rios, Hansol Bae, Jonatan Ulrik Fangel, Rituraj Batth, Jesper Harholt, Henrik Toft Simonsen
Summary: Regulation of cell division is crucial for the development of multicellular organisms. Physcomitrium differs from other plants by having cell division checks at both the G1 to S and G2 to M transition. Manipulating the expression of cell cycle regulators in Bryophytes can increase cell division without detrimental phenotypes, offering potential insights for plant growth regulation.
SCIENTIFIC REPORTS
(2023)
Review
Plant Sciences
Maria Ada Prusicki, Martina Balboni, Kostika Sofroni, Yuki Hamamura, Arp Schnittger
Summary: Live-cell imaging is a powerful tool for understanding cellular processes, especially in meiosis where chromosomes and cellular components exhibit complex dynamics over a short period of time. Recent advances in live-cell imaging now allow real-time analysis of meiotic events in plants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Hematology
Chenyan Wu, Daryl Boey, Oscar Bril, Jennine Grootens, M. S. Vijayabaskar, Chiara Sorini, Maria Ekoff, Nicola K. Wilson, Johanna S. Ungerstedt, Gunnar Nilsson, Joakim S. Dahlin
Summary: Mast cell accumulation is a characteristic of various diseases, and this study utilized single-cell transcriptomics analysis to determine the timing of FcεRI appearance during mast cell progenitor stage. External stimuli were also shown to regulate FcεRI expression in mast cell progenitors.
Article
Multidisciplinary Sciences
Chen Jia, Ramon Grima
Summary: The commonly used telegraph model for describing mRNA fluctuations in single cells does not consider the influence of cell cycle phase, cellular growth and division, and other crucial aspects of cellular biology. In this study, an extended telegraph model is developed to address these issues, taking into account the doubling of gene copy numbers upon DNA replication, dependence of mRNA synthesis rate on cellular volume, gene dosage compensation, partitioning of molecules during cell division, cell-cycle duration variability, and cell-size control strategies. The analytical time-dependent solution of this model is derived, and it reveals analytical distributions of transcript numbers for lineage and population measurements in steady-state growth, as well as a linear relation between mRNA fluctuations and cell volume fluctuations. The study also shows that accurate approximations of lineage and population distributions in steady-state growth cannot be achieved using the steady-state solution of extrinsic noise models, due to the presence of memory of division and replication in mRNA lifetime.
Article
Endocrinology & Metabolism
Hao Zhang, Renkai Wang, Guangchao Wang, Bo Zhang, Chao Wang, Di Li, Chen Ding, Qiang Wei, Zhenyu Fan, Hao Tang, Fang Ji
Summary: B cells in the bone marrow microenvironment play a crucial role in fracture healing by inhibiting excessive bone regeneration through the production of osteoblast inhibitors. Their numbers fluctuate during different stages of fracture healing, impacting the overall bone regrowth process.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Microbiology
Scott A. Hoose, Jimmy T. Trinh, Margaret Camille Leitch, Michelle M. Kelly, Ryan F. McCormick, Constantine L. Spyrou, Roger Smith, Michael Polymenis
FEMS MICROBIOLOGY LETTERS
(2013)
Article
Biochemistry & Molecular Biology
Andrew J. Steelman, Roger Smith, C. Jane Welsh, Jianrong Li
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Engineering, Environmental
Jasjeet Kaur, R. Karthikeyan, R. Smith
WATER SCIENCE AND TECHNOLOGY-WATER SUPPLY
(2013)
Article
Multidisciplinary Sciences
Juan Pablo Robles, Magdalena Zamora, Jose Luis Velasco-Bolom, Miriam Tovar, Ramon Garduno-Juarez, Thomas Bertsch, Gonzalo Martinez de la Escalera, Jakob Triebel, Carmen Clapp
SCIENTIFIC REPORTS
(2018)
Review
Cell Biology
Ivan Lazcano, Gabriela Hernandez-Puga, Juan Pablo Robles, Aurea Orozco
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2019)
Editorial Material
Endocrinology & Metabolism
Jakob Triebel, Juan Pablo Robles, Magdalena Zamora, Gonzalo Martinez de la Escalera, Thomas Bertsch, Carmen Clapp
FRONTIERS IN ENDOCRINOLOGY
(2019)
Article
Biochemical Research Methods
Bibiana Moreno-Carranza, Juan Pablo Robles, Hugo Cruces-Solis, Martha Gabriela Ferrer-Rios, Eduardo Aguilar-Rivera, Marco Yupanki, Gonzalo Martinez de la Escalera, Carmen Clapp
PROTEIN EXPRESSION AND PURIFICATION
(2019)
Article
Ophthalmology
Carlos D. Nunez-Amaro, Aura Ileana Moreno-Vega, Elva Adan-Castro, Magdalena Zamora, Renata Garcia-Franco, Paulina Ramirez-Neria, Marlon Garcia-Roa, Yolanda Villalpando, Juan Pablo Robles, Gabriela Ramirez-Hernandez, Mariana Lopez, Jorge Sanchez, Ellery Lopez-Star, Thomas Bertsch, Gonzalo Martinez de la Escalera, Ma Ludivina Robles-Osorio, Jakob Triebel, Carmen Clapp
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2020)
Article
Endocrinology & Metabolism
Nils Mueller, Juan Pablo Robles, Magdalena Zamora, Johannes Ebnet, Hulya Markl-Hahn, Gonzalo Martinez de la Escalera, Carmen Clapp, Thomas Bertsch, Jakob Triebel
Summary: Vasoinhibin, a protein hormone with key roles in vascular diseases, has been targeted for detection using monoclonal antibodies and ELISA, showing potential for diagnostic and therapeutic applications.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Peripheral Vascular Disease
Juan Pablo Robles, Magdalena Zamora, Lourdes Siqueiros-Marquez, Elva Adan-Castro, Gabriela Ramirez-Hernandez, Francisco Freinet Nunez, Fernando Lopez-Casillas, Robert P. Millar, Thomas Bertsch, Gonzalo Martinez de la Escalera, Jakob Triebel, Carmen Clapp
Summary: The study revealed that the hormone prolactin generates vasoinhibin with therapeutic potential against diseases, and identified the short linear motif HGR as the functional determinant which can effectively inhibit angiogenesis and vasopermeability.
Article
Biochemistry & Molecular Biology
Juan Pablo Robles, Magdalena Zamora, Elva Adan-Castro, Lourdes Siqueiros-Marquez, Gonzalo Martinez de la Escalera, Carmen Clapp
Summary: COVID-19 causes dysfunction of vascular endothelial cells, leading to edema, vascular inflammation, and coagulation. This study reveals that the spike protein of SARS-CoV-2 activates the inflammatory response in endothelial cells through binding with integrin alpha 5 beta 1, resulting in the expression of inflammatory and coagulation factors, as well as increased vascular permeability and leukocyte adhesion. These findings highlight integrin alpha 5 beta 1 as a potential target for treating vascular inflammation in COVID-19.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Cell Biology
Christin Friedrich, Leon Neugebauer, Magdalena Zamora, Juan Pablo Robles, Gonzalo Martinez de la Escalera, Carmen Clapp, Thomas Bertsch, Jakob Triebel
Summary: Plasmin was found to cleave human prolactin and placental lactogen to generate vasoinhibin-like peptides. The cleavage sites for prolactin and placental lactogen were identified at K170-E171 and R160-T161, respectively. Genetic variants of the human prolactin and placental lactogen genes are predicted to impact the proteolytic generation of these peptides.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2021)
Review
Endocrinology & Metabolism
Jakob Triebel, Juan Pablo Robles, Magdalena Zamora, Carmen Clapp, Thomas Bertsch
TRENDS IN ENDOCRINOLOGY AND METABOLISM
(2022)
Article
Toxicology
Roberto Ferriz-Martinez, Karina Garcia-Garcia, Lovanna Torres-Arteaga, Adriana Jheny Rodriguez-Mendez, Maria de Jesus Guerrero-Carrillo, Ulisses Moreno-Celis, Marco Vinicio Angeles-Zaragoza, Alejandro Blanco-Labra, Marco Alonso Gallegos-Corona, Juan Pablo Robles-Alvarez, Elizabeth Mendiola-Olaya, Hector Mario Andrade-Montemayor, Olga Patricia Garcia, Teresa Garcia-Gasca
TOXICOLOGY REPORTS
(2015)