Article
Multidisciplinary Sciences
Juyoung Kim, Gohta Goshima
Summary: Mitosis is a fundamental process in eukaryotes, and its essential genes can be bypassed through genetic or environmental changes. This study discovered the bypass of essentiality of a mitosis regulator, Polo-like kinase, through specific mutations and down-regulation of glucose uptake. The study also identified casein kinase I as an alternative mechanism for microtubule nucleation. The findings have implications for understanding mitosis and selecting chemotherapeutic compounds.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Fiorella Faienza, Federica Polverino, Girish Rajendraprasad, Giacomo Milletti, Zehan Hu, Barbara Colella, Deborah Gargano, Flavie Strappazzon, Salvatore Rizza, Mette Vixo Vistesen, Yonglun Luo, Manuela Antonioli, Valentina Cianfanelli, Caterina Ferraina, Gian Maria Fimia, Giuseppe Filomeni, Daniela De Zio, Joern Dengjel, Marin Barisic, Giulia Guarguaglini, Sabrina Di Bartolomeo, Francesco Cecconi
Summary: AMBRA1 is a key factor for nervous system development, primarily associated with autophagy and cell proliferation control. This study reveals that AMBRA1 is phosphorylated during mitosis and is critical for spindle function and orientation, driven by NUMA1 protein. The localization and dynamics of NUMA1 are dependent on AMBRA1 presence, phosphorylation, and binding ability. These findings suggest an additional role of AMBRA1 in tissue morphogenesis and differentiation, which could have implications for development and cancer oncogenesis.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Mariam Huda, Seyma Nur Bektas, Baris Bekdas, Ayse Koca Caydasi
Summary: This study reveals that lysosomal signalling lipid PI3,5P(2) plays a role in the timely execution of mitotic exit and promotes it by impairing Kin4.
Article
Biochemistry & Molecular Biology
Jiaqi Wen, Qiuke Wang, Wenyi Zhang, Weizhang Wang
Summary: This study reveals the upregulation of TUBA1A in GBM and its association with poor survival. Knockdown of TUBA1A inhibits mitotic progression and reduces tumor growth.
Article
Developmental Biology
Jiahui Du, Junjun Jing, Shuo Chen, Yuan Yuan, Jifan Feng, Thach-Vu Ho, Prerna Sehgal, Jian Xu, Xinquan Jiang, Yang Chai
Summary: The research reveals that Arid1a maintains tissue homeostasis by inhibiting the Aurka-Cdk1 axis, limiting proliferation of TACs, and promoting their differentiation, while loss of Arid1a leads to reduction of the MSC population.
Article
Biochemistry & Molecular Biology
Haiyu Song, Eun Ho Kim, Jihee Hong, Dasom Gwon, Jee Won Kim, Gyu-Un Bae, Chang-Young Jang
Summary: The centrosome forms a bipolar spindle during mitosis for faithful chromosome segregation. The DNA damage response (DDR) induces programmed spindle multipolarity and death in mitosis to prevent inheritance of DNA damage. Hornerin acts as a link between DDR and death in mitosis by forming a complex with checkpoint kinase 1 (Chk1) and polo-like kinase 1 (Plk1) to mediate phosphorylation at the polo-box domain (PBD) of Plk1. Hornerin mediates DDR-induced premature centriole disengagement through phosphorylation of Plk1 PBD, leading to spindle multipolarity. This study reveals the mechanism of how DDR eliminates mitotic cells with DNA damage to maintain genome integrity.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Plant Sciences
Zhengyu Shao, Shuhua Yang, Yinghui Gu, Yan Guo, Huapeng Zhou, Yongqing Yang
Summary: Ubiquitin directly binds to plant kinases SnRK2.2 and SnRK2.3 and regulates their activity in response to abscisic acid, revealing a previously unknown role for ubiquitin. This study provides important insights into the molecular basis of plant responses to complex environmental conditions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Article
Cell Biology
Chunlan Xu, Kunao Yang, Zuodong Xuan, Jinxin Li, Yankuo Liu, Yue Zhao, Zeyuan Zheng, Yang Bai, Zhiyuan Shi, Chen Shao, Lei Zhang, Huimin Sun
Summary: BCKDK is overexpressed in breast cancer and is associated with malignancy and metastasis. It promotes directed migration of breast cancer cells by disrupting cell adhesion and extracellular matrix formation and regulates the ubiquitination of talin1 and the activation of the FAK/MAPK pathway. BCKDK may serve as a biomarker for breast cancer metastasis and contribute to the development of new diagnostic markers and therapies.
CELL DEATH & DISEASE
(2023)
Article
Plant Sciences
Wei Lin, Yuehua Wang, Xinye Liu, Jian-Xiu Shang, Liqun Zhao
Summary: The rice wall-associated kinase (WAK) OsWAK112 negatively regulates plant salt responses by inhibiting ethylene production. OsWAK112 interacts with S-adenosyl-L-methionine synthetase (SAMS) 1/2/3, promoting OsSAMS1 degradation under salt stress, leading to decreased SAMS and ethylene content in plants under salt stress.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Veterinary Sciences
Huan Chen, Zhenzhong Wang, Xiaoyu Gao, Jiaxuan Lv, Yongxin Hu, Yong-Sam Jung, Shanyuan Zhu, Xiaodong Wu, Yingjuan Qian, Jianjun Dai
Summary: The ASFV-encoded lambda-like exonuclease pD345L acts as an inhibitor of cGAS/STING-mediated NF-kappa B signaling by blocking the IKK alpha/beta activity. It disrupts the activation of IFN beta and proinflammatory cytokines, leading to the inhibition of NF-kappa B signaling.
VETERINARY RESEARCH
(2022)
Article
Biochemical Research Methods
Rand Shahin, Nabil N. Al-Hashimi, Nour el-Huda Daoud, Salah Aljamal, Omar Shaheen
Summary: Targeting Polo-like kinase 1 (Plk1) using molecular inhibitors is a promising approach for tumor therapy. This study successfully identified a new QSAR model and pharmacophores for Plk1 inhibition, which were used to screen and validate new Plk1 inhibitory hits from a database.
JOURNAL OF MOLECULAR GRAPHICS & MODELLING
(2021)
Article
Biochemistry & Molecular Biology
Jamin B. Hein, Hieu T. Nguyen, Dimitriya H. Garvanska, Isha Nasa, Thomas Kruse, Yinnian Feng, Blanca Lopez Mendez, Norman Davey, Arminja N. Kettenbach, Polly M. Fordyce, Jakob Nilsson
Summary: Investigating the specificity of phosphoprotein phosphatases (PPPs) has been challenging, but this study introduces a novel approach, MRBLE:Dephos, to determine phosphatase preferences. Using this method, the authors identified amino acid preferences of residues surrounding the dephosphorylation site for PP1 and PP2A-B55, and discovered key dephosphorylation sites during mitotic exit that are consistent with MRBLE:Dephos results.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Cell Biology
Hiromi Maekawa, Shen Jiangyan, Kaoru Takegawa, Gislene Pereira
Summary: The mitotic exit network (MEN) is important for terminating mitosis in yeasts, but its molecular mechanisms differ between different species.
Article
Biology
Ahmed Abdelbaki, Camilla Ascanelli, Cynthia N. Okoye, H. Begum Akman, Giacomo Janson, Mingwei Min, Chiara Marcozzi, Anja Hagting, Rhys Grant, Maria De Luca, Italia Anna Asteriti, Giulia Guarguaglini, Alessandro Paiardini, Catherine Lindon
Summary: This research investigates the degradation mechanism of the mitotic kinase AURKA. The study finds that the C-terminal D-box of AURKA does not act as a degron but instead mediates essential structural features of the protein. It also shows that the N-terminal intrinsically disordered region of AURKA containing the A-box is sufficient for FZR1-dependent mitotic degradation. Additionally, the study suggests that the QRVL short linear interacting motif in the A-box may be a phospho-regulated D-box.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biochemistry & Molecular Biology
Sohini Basu, ProtitiMaiti Ghosh, Agamani Ghosal, Suchismita Datta, Geetanjali Sundaram
Summary: The bZIP transcription factor Atf1 plays a crucial role in the transcriptional programme of the cell cycle in Schizosaccharomyces pombe. It regulates the expression and degradation of mitotic cyclin Cdc13, and the temporal regulation of these functions is important for accurate cell division. Our study reveals that the activity of mitogen-activated protein kinase Spc1, along with the phosphorylation of Atf1 and the high activity of cyclin-dependent kinase Cdc2, regulate the degradation of Cdc13 during mitotic exit. We also propose a potential complex interplay between Wee1 kinase, the anaphase-promoting complex, and Atf1 in mitotic exit.