Article
Plant Sciences
Florence Ngo Ngwe, Sonja Siljak-Yakovlev
Summary: We studied the sex chromosomes of Dioscorea dumetorum in Cameroon and found that one pair of chromosomes is sex chromosomes. This research contributes to our understanding of the sex determination mechanism in D. dumetorum (standard sex-determining XX/XY system).
Article
Zoology
Arthur Mayrink Elizeu, Natalia Martins Travenzoli, Riudo de Paiva Ferreira, Denilce Meneses Lopes, Mara Garcia Tavares
Summary: This study analyzed the genomic organization of the Brazilian stingless bee Friesella schrottkyi, revealing specific patterns of chromosomes, intense spreading of microsatellites, and differences in 18S rDNA clusters among samples. The findings demonstrate the potential of molecular cytogenetics in understanding genetic variability in the Meliponini tribe.
ZOOLOGISCHER ANZEIGER
(2021)
Article
Biochemistry & Molecular Biology
Junhua Li, Jinjin Guo, Chenchen Wei, Yao Meng, Xiaoduan Wang, Panpan Yu, Lin Yang, Yi Liang, Siyi Guo, Jinhong Yuan
Summary: The development of genetic and genomic resources for biological studies in cucumber has led to a boom in recent years. This study provides cytological techniques for studying meiosis in cucumber, including identification of meiotic stages using hierarchical criteria and the development of a simplified fluorescence in situ hybridization (FISH) protocol. Comparative analysis of cytoplasmic organelle distribution between cucumber and Arabidopsis is also presented.
Article
Horticulture
Deen Mohammad Deepo, Md Mazharul Islam, Reshma Yesmin, Hye-Min Lee, Hong-Yul Kim, Chang-Kil Kim, Ki-Byung Lim
Summary: In this study, the nuclear DNA content, genome size, ploidy degree, and cytogenetic properties of two Korean native cultivars of Hibiscus 'Gangneung' and 'Baekryungdo' were determined. The flow cytometry results showed that the 2C-DNA contents of both cultivars were nearly identical. Fluorescence in situ hybridization (FISH) technique revealed the presence of 5S and 18S rDNA loci in both cultivars. This cytogenetic assessment of the cultivars will contribute to plant breeding within this genus.
HORTICULTURE ENVIRONMENT AND BIOTECHNOLOGY
(2023)
Article
Entomology
Mara Garcia Tavares, Riudo de Paiva Ferreira, Natalia Martins Travenzoli, Denilce Meneses Lopes
Summary: The study compared the karyotype, rDNA cluster number and localization, and repetitive DNA sequence distribution in different populations of T. spinipes from nine locations, showing diverse karyotypic formulas and heterochromatin preference for centromeric regions. Microsatellite probe signals were mainly located in euchromatic regions of all chromosomes, while 18S rDNA clusters varied in number among different chromosomal pairs. Cytogenetic variations were observed in T. spinipes along geographic distribution, particularly in scattered colonies.
Article
Genetics & Heredity
Kevin Santos da Silva, Larissa Glugoski, Marcelo Ricardo Vicari, Augusto Cesar Paes de Souza, Renata Coelho Rodrigues Noronha, Julio Cesar Pieczarka, Cleusa Yoshiko Nagamachi
Summary: The Ancistrus genus demonstrates extensive chromosomal diversity, including the occurrence of heteromorphic sex chromosomes and unstable repetitive sequences. These findings provide important insights into the evolution and genetic mechanisms of the Ancistrus genus.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Jie Huan, Zhoujian He, Yuting Lei, Wenjun Li, Liqiong Jiang, Xiaomei Luo
Summary: This study analyzed the genetic diversity of 16 Bletilla materials using SLAF-seq and FISH techniques. The results showed that the phylogenetic tree based on SNP data was correlated with the shape and geographical distribution of the materials. The population structure analysis revealed that materials with yellow labellum came from the same ancestor. Principal component analysis helped in determining genetic distance and provided a reference for hybrid parent selection. FISH analysis showed differences in chromosome characteristics and signals among the materials.
Article
Biochemistry & Molecular Biology
Analiza Fernanda Cavenagh, Matheus Pires Rincao, Felipe Cordeiro Dias, Antonio Domingos Brescovit, Ana Lucia Dias
Summary: The study reveals intra and interspecific chromosomal variability in the genus Lycosa, suggesting that chromosomal rearrangements are associated with the diversification of diploid number and sexual chromosome systems in this group of spiders.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Article
Genetics & Heredity
Gabriela Chrostek, Aleksandra Domaradzka, Alona Yurchenko, Lukas Kratochvil, Sofia Mazzoleni, Michail Rovatsos
Summary: This study applied both conventional and molecular cytogenetic analyses in seven species of geckos, revealing the presence of interstitial telomeric repeats in four species. However, the origin of these repeats remains unclear. Additionally, sex chromosomes were not detected in any examined species, suggesting the presence of poorly differentiated sex chromosomes or environmental sex determination. Future studies should investigate the impact of temperature and conduct genome-wide analyses to determine the mode of sex determination in these species.
Article
Plant Sciences
Anton Stepanenko, Guimin Chen, Phuong T. N. Hoang, Joerg Fuchs, Ingo Schubert, Nikolai Borisjuk
Summary: This study comprehensively characterized the chromosomal locations and molecular organization of 35S and 5S rDNA genes in water lettuce using karyological and molecular methods. Single locations for the 35S and 5S rDNA loci were identified using fluorescence in situ hybridization. The molecular cloning and nucleotide sequencing of 35S rDNA revealed typical structural characteristics and allowed for phylogenetic analysis. The 5S rDNA locus showed variation in nucleotide composition and subrepeat number, confirming the mosaic composition of the gene locus.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Ecology
Rodolpho S. T. Menezes, Diogo C. Cabral-de-Mello, Diogo Milani, Vanessa B. Bardella, Eduardo A. B. Almeida
Summary: Through molecular cytogenetic and genomic analysis, the main evolutionary trends of rDNA loci chromosomal distribution in Hymenoptera insects were identified. The study revealed the conservation of rDNA sites in haploid genomes and lineage-specific preferential locations.
JOURNAL OF EVOLUTIONARY BIOLOGY
(2021)
Article
Biology
Mariana Costa Terra, Fabio Hiroshi Takagui, Joana Neres Da Cruz Baldissera, Moema C. Costa De Lima, Eliana Feldberg, Ana L. Dias
Summary: This study focuses on the cytogenetic characteristics of Pimelodella cristata, a species within the Heptapteridae family, and discusses the mechanisms behind the karyotypic variability of the genus.
Article
Genetics & Heredity
Lorenzo Clemente, Sofia Mazzoleni, Eleonora Pensabene, Tomas Protiva, Philipp Wagner, Uwe Fritz, Lukas Kratochvil, Michail Rovatsos
Summary: The Asian box turtle genus Cuora consists of 13 species distributed in Southeast Asia. The populations of these species are rapidly declining due to human activities. Research shows that all species have a diploid chromosome number of 2n = 52, with similar karyotypes and poorly differentiated sex chromosomes.
Article
Entomology
Laura Ruiz-Torres, Pablo Mora, Areli Ruiz-Mena, Jesus Vela, Francisco J. Mancebo, Eugenia E. Montiel, Teresa Palomeque, Pedro Lorite
Summary: Blister beetles in the family Meloidae are well known for secreting cantharidin, which has sparked interest in its anticancer potential. However, cytogenetic and molecular data in this group are limited. This study conducted karyotype and DAPI staining analyses on Hycleus scutellatus, revealing its chromosome number, sex chromosomes, and mapping of rDNA clusters. Additionally, a satellite DNA family was isolated and identified as potentially one of the most abundant satellite DNA repeats in H. scutellatus.
Article
Agronomy
Kai Wang, Hui Zhang, Haris Khurshid, Ayman Esh, Caiwen Wu, Qinnan Wang, Nathalie Piperidis
Summary: This paper reviews the application and progress of cytogenetic techniques in the genus Saccharum, and discusses the future applications of cytogenetics in genomic studies and breeding of sugarcane and other plants with complex genomes.
Article
Biochemistry & Molecular Biology
Prasit Boonthai, Kowit Noikotr, Naraphatra Saemram, Runglawan Sudmoon, Tawatchai Tanee, Arunrat Chaveerach, Natcha Patarapadungkit, Pornnarong Siripiyasing
Summary: Based on the long history of medicinal use, the extract formulations of Thunbergia laurifolia, Clerodendrum disparifolium, and Rotheca serrata showed high detoxification efficacies in human cell and tissue cultures. Formulations 1 and 2 had higher detoxification levels than formulation 3, especially when administered immediately after exposure to toxic substances.
CURRENT PHARMACEUTICAL BIOTECHNOLOGY
(2022)
Article
Genetics & Heredity
Abdulsamad Wafa, Belal Ali, Faten Moassass, Maged Kheder, Abdulmunim Aljapawe, Bassel Al-Halabi, Kristin Mrasek, Thomas Liehr, Walid Al-Achkar
Summary: This study investigated the CEBPA gene mutations in a Syrian family with AML. The results showed that all affected family members had a good response to chemotherapy and achieved complete remission. This research is important for understanding the relationship between CEBPA gene mutations and AML.
MOLECULAR GENETICS & GENOMIC MEDICINE
(2022)
Article
Genetics & Heredity
Sanit Kaewdaungdee, Runglawan Sudmoon, Tawatchai Tanee, Shiou Yih Lee, Arunrat Chaveerach
Summary: By investigating the chloroplast genomes of five Barleria species, it was found that they had differences in genome structure, gene content, and genetic distance. The genetic relationships between species were determined using chloroplast genomes and CDS phylogenetic trees. These results are important for authenticating the five Barleria species and the six genera in Acanthaceae.
Article
Environmental Sciences
Sampan Tongnunui, Treerat Sooksawat, Charoonroj Chotwiwatthanakun, Weerayuth Supiwong, Amnuay Wattanakornsiri, F. W. H. Beamish
Summary: Seasonal change affects the Critical thermal maximum (CTmax) of riverine fishes, with lower thermal tolerance in the wet season and higher tolerance in the dry season. The variations in CTmax for the four studied fish species can be attributed to their seasonal plasticity in response to thermal stress. R. caudimaculata and M. chilopterus show higher capacities to tolerate heat stress across both wet and dry seasons.
Article
Biochemistry & Molecular Biology
Gustavo A. Toma, Natalia dos Santos, Rodrigo dos Santos, Petr Rab, Rafael Kretschmer, Tariq Ezaz, Luiz A. C. Bertollo, Thomas Liehr, Fabio Porto-Foresti, Terumi Hatanaka, Alongklod Tanomtong, Ricardo Utsunomia, Marcelo B. Cioffi
Summary: The study investigated the karyotypes of different color phenotypes in Scleropages formosus using molecular cytogenetic techniques. The results revealed population genetic structure and microstructure differences in the karyotypes of the color phenotypes, but did not conclusively support the hypothesis of discrete lineages or evolutionary units among them.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dagmar Heydeck, Christoph Ufer, Kumar R. Kakularam, Michael Rothe, Thomas Liehr, Philippe Poulain, Hartmut Kuhn
Summary: The researchers created transgenic mice expressing human ALOX15 under the control of the aP2 promoter, which showed high expression in adipocytes, bone marrow cells, and peritoneal macrophages. The catalytic activity of the transgenic enzyme was confirmed through activity assays and plasma metabolite analysis. The aP2-ALOX15 mice exhibited gender-specific differences in body-weight kinetics compared to the wildtype controls.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Genetics & Heredity
Warin Wonok, Runglawan Sudmoon, Tawatchai Tanee, Shiou Yih Lee, Arunrat Chaveerach
Summary: Through studying the chloroplast genomes of four Dioscorea species, the researchers found that the genome size ranged from 153,370 to 153,503 bp, with a total of 113 predicted genes, including 79 protein-coding sequences, 30 tRNA, and four rRNA genes. The overall GC content was 37%, and only mono-, di-, and trinucleotides were present in the genome. Analysis of the chloroplast genome alignment revealed eight distinct indel variations, and a sliding window analysis identified three highly variable regions. The phylogenetic tree constructed based on the complete chloroplast genome sequence provided valuable insights into the relationships and genetic identity of Dioscorea species.
Article
Entomology
Jhon A. D. Vidal, Francisco de M. C. Sassi, Renata L. R. de Moraes, Roberto F. Artoni, Thomas Liehr, Marcelo B. Cioffi, Mara C. de Almeida
Summary: In this study, the similarity of X and Y chromosomes in four Omophoita species was examined, and genomic differentiation was compared to better understand the evolutionary process of the giant sex chromosomes. The results showed significant genomic similarity between the sexes and a sex-specific region on the Y chromosome, as well as genomic divergence between species. However, whole chromosome painting experiments revealed high intra- and interspecific similarity of the sex chromosomes in O. octoguttata. Our data support a common origin and canonical evolution of the sex chromosomes in this group, as they have high genomic similarity between them.
Review
Biochemistry & Molecular Biology
Galina Hovhannisyan, Tigran Harutyunyan, Rouben Aroutiounian, Thomas Liehr
Summary: Cell-free DNA (cfDNA) has become a commonly used diagnostic marker in various pathologies, including viral infections like COVID-19, due to its higher sensitivity compared to standard biochemical parameters. This review focuses on the identification, sources, molecular features, and mechanisms of release and clearance of cfDNA. It discusses the general suitability of cfDNA for diagnostic purposes, potential limitations, and future directions, with a special emphasis on coronavirus infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Francisco de Menezes Cavalcante Sassi, Alexandr Sember, Geize Aparecida Deon, Thomas Liehr, Niklas Padutsch, Osvaldo Takeshi Oyakawa, Marcelo Ricardo Vicari, Luiz Antonio Carlos Bertollo, Orlando Moreira-Filho, Marcelo de Bello Cioffi
Summary: The study investigates the chromosomal rearrangements and associated synteny blocks in the origin of a multiple X1X2Y sex chromosome system in the Neotropical catfish genus Harttia. The results suggest that the X1X2Y sex chromosome systems in H. punctata, H. duriventris and H. villasboas represent the same linkage groups which also form the putative XY sex chromosomes of H. rondoni. The study also indicates that the X1X2Y sex chromosome system may have formed through an X chromosome fission rather than a previously proposed Y-autosome fusion.
SCIENTIFIC REPORTS
(2023)
Article
Biology
Mariannah Pravatti Barcellos de Oliveira, Rafael Kretschmer, Geize Aparecida Deon, Gustavo Akira Toma, Tariq Ezaz, Caio Augusto Gomes Goes, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: In this study, the dynamics of satellite DNAs (SatDNAs) accumulation and elimination during W chromosome differentiation in Triportheus fish were investigated. The majority of repetitive sequences were eliminated as speciation occurred in five additional Triportheus species. While the W chromosomes continued to degenerate, the Z chromosomes in some species began to accumulate TauSatDNAs. Species-specific SatDNAs were likely amplified in the heterochromatic regions of both Z and W chromosomes in each species. Thus, the W chromosomes of various Triportheus species underwent significant evolutionary changes within a short period of time (15-25 Myr) after divergence.
Article
Infectious Diseases
Natapol Pumipuntu, Tawatchai Tanee, Penkhae Thamsenanupap, Pensri Kyes, Apichat Karaket, Randall C. Kyes
Summary: This study aimed to assess the molecular characteristics of MRSA, MSSA, and S. argenteus strains in free-ranging long-tailed macaques in Thailand. PCR analysis revealed that clfA, coa, and the spa IgG binding region were the most prevalent virulence genes. These findings suggest that wild macaques may act as carriers for virulent staphylococcal bacteria distribution in the study area.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2023)
Article
Infectious Diseases
Natapol Pumipuntu, Tawatchai Tanee, Pensri Kyes, Penkhae Thamsenanupap, Apichat Karaket, Randall C. Kyes
Summary: This study investigates the prevalence of Leptospirosis in wild long-tailed macaques at Kosumpee Forest Park, Thailand. The results suggest that the macaques in this area may act as natural reservoirs for Leptospirosis, and several pathogenic Leptospira serovars are circulating in the wild macaques.
INFECTIOUS DISEASE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Renata Luiza Rosa de Moraes, Francisco de Menezes Cavalcante Sassi, Jhon Alex Dziechciarz Vidal, Caio Augusto Gomes Goes, Rodrigo Zeni dos Santos, Jose Henrique Forte Stornioli, Fabio Porto-Foresti, Thomas Liehr, Ricardo Utsunomia, Marcelo de Bello Cioffi
Summary: Chromosomal rearrangements play a significant role in the evolution of fish genomes, and satellite DNA sequences have potential relevance in both sex and karyotype evolution. This study investigated the association of satDNAs with chromosome-shuffling events and their involvement in the evolution of sex chromosomes in Pyrrhulina fish. In addition, a large collection of satDNAs for both species was characterized for the first time, and their potential involvement in karyotype evolution was demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Geize Aparecida Deon, Larissa Glugoski, Terumi Hatanaka, Francisco de Menezes Cavalcante Sassi, Viviane Nogaroto, Luiz Antonio Carlos Bertollo, Thomas Liehr, Ahmed Al-Rikabi, Orlando Moreira Filho, Marcelo de Bello Cioffi, Marcelo Ricardo Vicari
Summary: This study investigates the chromosomal rearrangements in the Neotropical armored catfish genus Harttia using powerful tools such as comparative whole chromosome painting. The results reveal the importance of translocation events in the karyotype evolution of Harttia species, as well as the independent origin of two types of multiple sex chromosomes.
GENETICS AND MOLECULAR BIOLOGY
(2022)