Article
Biochemistry & Molecular Biology
Yunyou Nan, Yuyu Xie, Ayub Atif, Xiaojun Wang, Yanfeng Zhang, Hui Tian, Yajun Gao
Summary: This study identified 23 SLAC/SLAH genes in rapeseed and classified them into three main groups based on structural characteristics and phylogenetic analysis. The transcriptome data showed that BnSLAH3 genes were expressed in various tissues of rapeseed and could be up-regulated by low nitrate treatment in roots. In transient expression of tobacco leaves, BnSLAC/SLAHs were exclusively localized on the plasma membrane.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Run-Jie Du, Ze-Xuan Wu, Zhao-Xi Yu, Peng-Feng Li, Jian-Yu Mu, Jie Zhou, Jia-Na Li, Hai Du
Summary: This study identified the NRT2 gene family in two sequenced Brassica napus ecotypes and found its essential role in nitrogen uptake, transport, utilization, and stress resistance. The candidate genes were divided into three groups based on phylogenetic analysis and shared a conserved intron-exon structure in each group. Collinearity analysis revealed the expansion of BnaZSNRT2s was due to allopolyploidization and small-scale duplication events. The expressions of BnaZSNRT2s were regulated by multiple factors and showed conserved patterns within and between groups. The diverse spatial-temporal expression profiles were observed in Group I, while conserved patterns were observed in Group II/III. Low nitrogen stress up-regulated the expression profiles in all three groups.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Ali Raza, Wei Su, Ziqi Jia, Dan Luo, Yi Zhang, Ang Gao, Muhammad Azhar Hussain, Sundas Saher Mehmood, Yong Cheng, Yan Lv, Xiling Zou
Summary: Trehalose acts as a growth modulator and improves cold stress tolerance in rapeseed seedlings. The study found that exogenous trehalose mitigates the adverse effects of cold stress on rapeseed seedlings, increasing their survival rate. Trehalose regulates antioxidant and osmotic balance, participates in metabolism and signaling networks to enhance cold stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Lei Yan, Liu Zeng, Ali Raza, Yan Lv, Xiaoyu Ding, Yong Cheng, Xiling Zou
Summary: This study aimed to investigate the beneficial role of exogenous inositol in improving cold stress tolerance in rapeseed. The results revealed that inositol positively regulated cold tolerance by increasing calcium ion influx in rapeseed. The inositol-Ca2+ pathway was found to play a key role in cold tolerance. This study provides new insights into the mechanism of rapeseed cold tolerance and introduces a feasible method for improving rapeseed cold tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Diana Saja-Garbarz, Agnieszka Ostrowska, Katarzyna Kaczanowska, Franciszek Janowiak
Summary: The study found that silicon accumulates even in non-accumulator species like oilseed rape under certain conditions, and potentially improves water uptake under drought stress.
Article
Plant Sciences
Xingzhi Qian, Wenyin Zheng, Jian Hu, Jinxu Ma, Mengyuan Sun, Yong Li, Nian Liu, Tianhua Chen, Meiqi Wang, Ling Wang, Xinzhe Hou, Qingao Cai, Zhaoshun Ye, Fugui Zhang, Zonghe Zhu
Summary: In this study, 26 BnDFR genes were identified and analyzed for their physicochemical properties, subcellular localization, and conserved structure. The evolutionary relationship, collinearity analysis, and expression characteristics of these genes were studied, as well as the correlation between their expression levels and anthocyanin content in rape petals. The results showed that these genes were distributed in different subcellular compartments, chromosomes, and groups, and certain genes may play a key role in anthocyanin synthesis in rape petals.
Article
Multidisciplinary Sciences
Wenbo Mi, Zigang Liu, Jiaojiao Jin, Xiaoyun Dong, Chunmei Xu, Ya Zou, Mingxia Xu, Guoqiang Zheng, Xiaodong Cao, Xinling Fang, Caixia Zhao, Chao Mi
Summary: The study compared two winter rapeseed cultivars, NS and NF, under cold stress and found that NS exhibited higher antioxidant activity and osmotic adjustment ability, and less plasma membrane injury compared to NF. Proteomic analysis revealed that NS accumulated proteins related to ROS scavenging more rapidly in lysine degradation, phenylalanine, tyrosine, and tryptophan, flavonoid biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis pathways, enhancing cold tolerance and minimizing ROS damage.
Article
Plant Sciences
Meng Ma, Wenying Zhong, Qing Zhang, Li Deng, Jing Wen, Bin Yi, Jinxing Tu, Tingdong Fu, Lun Zhao, Jinxiong Shen
Summary: This study compared the epigenome and transcriptome data of a hybrid and its parental lines in different tissues to explore their contribution to heterosis in allopolyploid B. napus. The study found that a small proportion of non-additive genes in the hybrid, as well as parental expression level dominance, had a significant effect on heterosis. Histone modification variations were observed between the parents and hybrid, with H3K4me3 variations positively correlated with gene expression differences. These findings provide valuable insights into the understanding of heterosis in allopolyploid crops.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biotechnology & Applied Microbiology
Shan Tang, Ning Guo, Qingqing Tang, Fei Peng, Yunhao Liu, Hui Xia, Shaoping Lu, Liang Guo
Summary: The study highlights the important role of BnaBASS2 in seed lipid metabolism and accumulation in Brassica napus, showing that gene manipulation can alter metabolites in seeds and influence lipid synthesis.
PLANT BIOTECHNOLOGY JOURNAL
(2022)
Article
Plant Sciences
Meimei Hu, Zengde Xi, Jianbo Wang
Summary: This study utilized transcriptome, WGBS, and Chip-Seq sequencing data to explore the regulatory consequences of DNA methylation and histone modifications on gene expression in allotetraploid Brasscia napus. The results demonstrated that DNA methylation is crucial for gene expression regulation, and different epigenetic modifications have an essential function in regulating the differential expression of genes in B. napus.
Article
Biochemistry & Molecular Biology
Javier Canales, Jose Verdejo, Gabriela Carrasco-Puga, Francisca M. Castillo, Anita Arenas-M, Daniel F. Calderini
Summary: This study conducted a transcriptomic analysis of the relationship between seed weight and number in two rapeseed genotypes, revealing that a reduction in the source-sink ratio induced the expression of specific gene groups related to sucrose transport, seed weight, and stress responses. Additionally, the research identified co-expression modules correlated with seed weight and negatively correlated with seed number, with a significant enrichment of transcription factors in one module. Network analysis predicted NAC transcription factors as key regulators of seed weight and number compensation in rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Yafang Xiao, Mengdi Li, Jianbo Wang
Summary: This study evaluates the number, structure, phylogeny, and expression of MBD genes in Brassica napus and its diploid ancestors, providing a deeper understanding of MBD genes in allopolyploids and serving as a reference for future analyses of allopolyploidization.
Article
Plant Sciences
Lu Zhai, Lingli Xie, JinSong Xu, Benbo Xu, Jing Dong, XueKun Zhang
Summary: This study investigated the effects and mechanisms of Thidiazuron (TDZ) treatment on seed size of Brassica napus, providing important insights for improving rapeseed yield.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Weixian Zhou, Libin Zhang, Jianjie He, Wang Chen, Feifan Zhao, Chunhua Fu, Maoteng Li
Summary: Interspecific crosses between Brassica rapa and B. napus result in transcriptome shock in the developing seeds, leading to overall changes in gene expression patterns. The hybrids show expression bias towards B. rapa and significant changes in key transcription factors, which may be responsible for phenotype change.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Qing Zhang, Pengpeng Guan, Lun Zhao, Meng Ma, Liang Xie, Yue Li, Ruiqin Zheng, Weizhi Ouyang, Shunyao Wang, Hongmeijuan Li, Ying Zhang, Yong Peng, Zhilin Cao, Wei Zhang, Qin Xiao, Yuanling Xiao, Tingdong Fu, Guoliang Li, Xingwang Li, Jinxiong Shen
Summary: The study conducted a comprehensive analysis of the epigenome in two Brassica napus lines, revealing significant differences between the An and Cn subgenomes and distinct patterns of histone modification and DNA methylation in duplicated genes. The findings provide valuable insights into epigenetic regulation in allopolyploid plants.