期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 106, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/53384
关键词
Cellular Biology; Issue 106; Blood outgrowth endothelial cell (BOEC); endothelial progenitor cell (EPC); induced pluripotent stem cells (iPSCs)
资金
- British Heart Foundation (BHF)
- Dinosaur Trust
- McAlpine Foundation
- Fondation Leducq
- Fight for Sight
- Cambridge Biomedical Research Centre, National Institute of Health Research including BHF Oxbridge Centre of Regenerative Medicine [RM/13/3/30159]
- Cambridge Biomedical Research Centre, National Institute of Health Research including BHF Cambridge Centre of Research Excellence
- Cambridge Biomedical Research Centre, National Institute of Health Research including Addenbrooke's Hospital, Cambridge University Hospitals NHS Foundation Trust
- Cambridge Biomedical Research Centre, National Institute of Health Research including Papworth Hospital NHS Foundation Trust
- Cambridge NIHR BRC Cell Phenotyping Hub
- BHF Intermediate Fellowship
- BHF PhD Studentship
- MRC [G0800784, G1000847, MR/K020919/1] Funding Source: UKRI
- British Heart Foundation [FS/12/39/29653, SP/12/12/29836, PG/14/31/30786, PG/10/35/28339, RG/13/4/30107] Funding Source: researchfish
- Medical Research Council [G1000847, MR/K020919/1, G0800784] Funding Source: researchfish
- National Institute for Health Research [NF-SI-0514-10086] Funding Source: researchfish
Historically, the limited availability of primary endothelial cells from patients with vascular disorders has hindered the study of the molecular mechanisms underlying endothelial dysfunction in these individuals. However, the recent identification of blood outgrowth endothelial cells (BOECs), generated from circulating endothelial progenitors in adult peripheral blood, may circumvent this limitation by offering an endothelial-like, primary cell surrogate for patient-derived endothelial cells. Beyond their value to understanding endothelial biology and disease modeling, BOECs have potential uses in endothelial cell transplantation therapies. They are also a suitable cellular substrate for the generation of induced pluripotent stem cells (iPSCs) via nuclear reprogramming, offering a number of advantages over other cell types. We describe a method for the reliable generation, culture and characterization of BOECs from adult peripheral blood for use in these and other applications. This approach (i) allows for the generation of patient-specific endothelial cells from a relatively small volume of adult peripheral blood and (ii) produces cells that are highly similar to primary endothelial cells in morphology, cell signaling and gene expression.
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