期刊
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
卷 -, 期 102, 页码 -出版社
JOURNAL OF VISUALIZED EXPERIMENTS
DOI: 10.3791/53077
关键词
Immunology; Issue 102; Intravital microscopy; inflammation; mesentery vessels; leukocyte-endothelial interactions; platelet-leukocyte interactions; mouse model
资金
- Deutsche Forschungsgemeinschaft [DU 1190/3-1]
Intravital microscopy is a method that can be used to investigate different processes in different regions and vessels in living animals. In this protocol, we describe intravital microscopy of mesentery veins. This can be performed in a short period of time with reproducible results showing leukocyte-endothelial interactions in vivo. We describe an inflammatory setting after LPS challenge of the endothelium. But in this model one can apply many different types of inflammatory conditions, like bacterial, chemical or biological and investigate the administration of drugs and their direct effects on the living animal and its impact on leukocyte recruitment. This protocol has been applied successfully to a number of different treatments of mice and their effects on inflammatory response in vessels. Herein, we describe the visualization of leukocytes and platelets by fluorescently labeling these with rhodamine 6G. Additionally, any specific imaging can be performed using targeted fluorescently labeled molecules.
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