Article
Biochemistry & Molecular Biology
Gabriele Minazzato, Elisa Marangoni, Carlo Fortunato, Riccardo Petrelli, Loredana Cappellacci, Fabio Del Bello, Leonardo Sorci, Massimiliano Gasparrini, Francesco Piacente, Santina Bruzzone, Nadia Raffaelli
Summary: Maintaining an adequate NAD(+) pool is crucial for cell survival, especially in tumor cells. Inhibition of NAD(+) biosynthesis through targeting enzymes such as NAMPT and NAPRT is considered a promising therapeutic strategy. A new continuous coupled fluorometric assay was developed to screen NAPRT inhibitors by measuring NADH formation. Several compounds were identified as NAPRT inhibitors and were shown to sensitize cancer cells to NAMPT inhibition, decreasing viability and intracellular NAD(+) levels.
Article
Biotechnology & Applied Microbiology
Frederic M. Lapierre, Isabel Bolz, Jochen Buechs, Robert Huber
Summary: This article presents a microplate assay for quantifying urease activity using a fluorescent pH indicator, which is suitable for automated measurements. The assay allows for non-hazardous and reliable measurements without the need for sample preparation. It is important for research on Microbial Induced Calcite Precipitation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Jiabao Zhang, Jinying Liu, Mengke Wang, Guannan Wang, Xingguang Su
Summary: A sensitive fluorescence strategy was developed for the detection of alpha-glucosidase activity using AgInZnS quantum dots. The fluorescence of AIZS QDs was quenched and recovered through the oxidation-reduction process of Ce4+ and PPD. The detection system showed a good linear relationship and a low detection limit, making it a competitive alternative for alpha-glucosidase-related diagnostics.
Article
Microbiology
Anqi Chen, Danhui Wang, Sam R. Nugen, Juhong Chen
Summary: Utilizing an enzyme-based fluorescent assay, the study demonstrated a sensitive method for detecting the presence of E. coli in food samples, providing a straightforward approach to detect potential biological contamination. The engineered phages showed a detection limit of 10 CFU/mL and could be applied to detect E. coli strains from environmental samples.
Article
Biochemistry & Molecular Biology
Cheng-Yu Wang, Chih-Ming Chou, Cheng-Ying Chu, Amy Chen, En-Hsin Liu, Cheng-Yang Liu, Yu-Lin Amy Lee, Fwu-Long Mi, Chia-Hsiung Cheng
Summary: In this study, low-molecular-weight chitosan (LMWCS) was used to release reporter proteins in zebrafish, providing potential for detection. A simple fluorometric-based assay using LMWCS and transgenic zebrafish was developed to evaluate clinical antiangiogenic drugs. Treatment with the antiangiogenic drug sorafenib significantly reduced angiogenesis in zebrafish, as indicated by the LMWCS fluorometric-based assay. Evaluation of other clinical drugs also showed a significant reduction in angiogenesis. Overall, this study demonstrated the utility of the LMWCS fluorometric-based assay for evaluating angiogenic drugs in zebrafish.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Ruhani Sagar, Jinchao Lou, Michael D. Best
Summary: In this work, a bis-pyrene phospholipid probe was designed, synthesized, and applied for the detection of phospholipase A2 (PLA2) activity. The probe exhibited changes in pyrene monomer and excimer fluorescence intensities upon PLA2 action. Continuous fluorometric assays enabled the detection of multiple PLA2 enzymes and the inhibition of PLA2 activity by p-bromo phenacylbromide. Thin-layer chromatography and mass spectrometry analysis confirmed the hydrolysis of the probe by PLA2. The probe also showed cleavage by phospholipase C and D enzymes, although fluorescence changes were not observed. Overall, the developed bis-pyrene phospholipid probe is effective for the detection of PLA2 activity and can be used for inhibitor screening.
BIOORGANIC & MEDICINAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Ming Li, Chenfei Han, Chuqin Yang, Mingna Sun, Jinsheng Duan, Xuchun Qiu, Daolin Du
Summary: A ultrasensitive fluorometric oligonucleotide immunoassay (UFOIA) based on a fluorometric oligonucleotide and magnetic separation was proposed for simultaneous detection of two mycotoxins, exhibiting improved sensitivities compared to ELISA.
ANALYTICAL METHODS
(2022)
Article
Chemistry, Medicinal
David K. Heidary, Sarah M. Kriger, Austin C. Hachey, Edith C. Glazer
Summary: This study introduces a novel cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates in live cells.
Article
Environmental Sciences
Jagadeesh Kumar Alagarasan, Siddharthy Shasikala, Sivarasan Ganesan, Manimekalan Arunachalam, Utaiyachandran Manojkumar, Senthilkumar Palaninaicker, Dinh Duc Nguyen, Soon Wong Chang, Moonyong Lee, Huang-Mu Lo
Summary: Silicon nanoparticles (Si NPs) with exceptional optical properties have been prepared from a 3-aminopropyl trimethoxysilane precursor in a one-pot process. The Si NPs exhibit excitation-wavelength-dependent fluorescence properties and have been used to develop a sensitive fluorometric turn-off sensor for cyanide ion detection. The detection assay based on Si NPs shows a good linear response and a low limit of detection.
ENVIRONMENTAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Meghna Chakraborty, Srividya Shivakumar
Summary: This study identified a potentially valuable L-asparaginase from the less explored Agaricomycetes bacteria. Through analysis and testing, it was found to have high activity and be free of L-glutaminase and urease, making it a promising candidate for pharmaceutical and food industries.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Jinying Liu, Jiabao Zhang, Mengke Wang, Xingguang Su
Summary: A sensitive dual-mode assay using silicon quantum dots and nitrobenzoic acid was designed to effectively detect D-penicillamine. The assay showed promising results in detecting D-PA in both laboratory and real biological samples.
Article
Food Science & Technology
Yanbo Wang, Linfang Li, Huan Li, Yafei Peng, Linglin Fu
Summary: A sandwich biosensor using magnetic particles and carbon dots was proposed for the detection of TM in seafood products, with a detection limit of 30.76 ng mL(-1). The method demonstrated excellent specificity and accuracy in detecting TM in commercial samples, showing great potential for applications.
Article
Biophysics
Jia-Qi Li, Yan-Wen Mao, Rui Zhang, Ai-Jun Wang, Jiu-Ju Feng
Summary: This study developed a rapid, cost-effective, and simple method for sensitive dual-mode detection of cholesterol in serum samples. By incorporating Fe-Ni dual-single-atom nanozyme into N-doped carbon nanosheets, enzyme activity was enhanced. This method allows simultaneous determination of hydrogen peroxide and cholesterol.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Chemistry, Physical
Mahsa Abbasi-Azad, Farzaneh Rouhani, Ali Morsali
Summary: The amine functionalized metal-organic framework TMU-60 showed potential for selective detection of Cr3+ ions in aqueous solution, with a detection limit of 0.89 nM for nano TMU-60. A significant impact of structure size on sensing performance was observed, demonstrating amine's effectiveness for sensing Cr3+ ions.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Chemistry, Analytical
Anastasiya A. Mamaeva, Vladimir I. Martynov, Sergey M. Deyev, Alexey A. Pakhomov
Summary: This study directly compared different dyes and assays for protein concentration determination. The results showed that the classical Bradford assay achieved excellent results compared to modern assays. Additionally, standard approaches for determining the limit of detection and limit of quantification may not work correctly with certain fluorescent dyes, leading to the proposal of a new extended method.
