Article
Microbiology
Emma C. L. Marrs, Audrey Perry, John D. Perry
Summary: This study compared the efficacy of three commercially available selective media for the isolation of Burkholderia cepacia complex (BCC). The results showed that all three media were effective for BCC isolation, with BCSA being more selective than the other two media.
Article
Microbiology
Holly K. Huse, Mark J. Lee, Mandy Wootton, Susan E. Sharp, Maria Traczewski, John J. LiPuma, Peter Jorth
Summary: This study evaluated the reproducibility and accuracy of different antimicrobial susceptibility testing methods for Burkholderia cepacia complex (BCC). The findings showed poor accuracy for disk diffusion and MicroScan WalkAway methods, while broth microdilution method demonstrated higher reproducibility for meropenem and minocycline.
JOURNAL OF CLINICAL MICROBIOLOGY
(2021)
Article
Immunology
Edgard de Freitas Vianna, Nicoli Izzy Miotto Menezes, Mila Muraro de Almeida, Fla'via Alvim Dutra de Freitas, Cassiana da Costa Ferreira Leite, Monica Cassia Firmida, Davi Ribeiro Iglesias de Medeiros, Robson Souza Leao, Rodolpho Mattos Albano, Elizabeth Andrade Marques
Summary: The genome analysis of Burkholderia cenocepacia complex III revealed high virulence and antimicrobial resistance, with a predominance of genes related to invasion mechanisms and flagellar biosynthesis. Additionally, the isolate exhibited bacterial dissemination mechanisms with invasive determinants.
Article
Microbiology
Shekooh Behroozian, James E. A. Zlosnik, Wanjing Xu, Loretta Y. Y. Li, Julian E. Davies
Summary: There is a crisis in healthcare due to antibiotic-resistant bacteria, necessitating the search for novel antimicrobial agents. Kisameet clay has been found to inhibit acute infections, presenting a potential therapy. This offers hope for addressing the challenge of chronic bacterial infections and improving treatment options.
Article
Respiratory System
C. Olcese, R. Casciaro, D. Pirlo, C. Debbia, E. Castagnola, F. Cresta, C. Castellani
Summary: In this case, a CF patient was co-infected with COVID-19 and Bc, and was treated with antibiotics, dexamethasone, remdesivir, and heparin, leading to gradual improvement and discharge. The reciprocal role of SARS-CoV-2 and Bc, as well as the impact of individual therapies on the outcome, remain unclear.
JOURNAL OF CYSTIC FIBROSIS
(2021)
Article
Immunology
Samuele Irudal, Viola Camilla Scoffone, Gabriele Trespidi, Giulia Barbieri, Maura D'Amato, Simona Viglio, Mariagrazia Pizza, Maria Scarselli, Giovanna Riccardi, Silvia Buroni
Summary: The Burkholderia cepacia complex is a group of Gram-negative bacteria that infects people with weakened immune systems, such as those with cystic fibrosis. Due to their high level of antibiotic resistance, commonly used treatments are often ineffective, leading to worse outcomes and the spread of multi-drug resistance. In this study, reverse vaccinology was used to identify potential antigens for vaccination. Three proteins (BCAL1524, BCAM0949, and BCAS0335) were investigated for their localization and virulence-related properties. The findings suggest that these proteins play important roles in the virulence of Burkholderia cepacia complex and further research is needed to assess their potential as antigen candidates.
Article
Multidisciplinary Sciences
Olakunle A. Jaiyesimi, Andrew C. McAvoy, David N. Fogg, Neha Garg
Summary: This study compared the metabolomes of three clinical B. cenocepacia strains in synthetic CF sputum medium and a routine laboratory medium, revealing differential production of compound classes and specific metabolites in the presence of the antibiotic trimethoprim. The findings provide insight into environment-dependent production of secondary metabolites by B. cenocepacia strains and suggest future research directions to identify personalized strain-specific regulatory mechanisms. Investigations into whether antibiotics with different mechanisms of action induce similar metabolic alterations could inform development of combination treatments for effective clearance of Burkholderia spp. pathogens.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Chanu Rhee, Meghan A. Baker, Robert Tucker, Vineeta Vaidya, Meghan Holtzman, Raghu R. Seethala, Maria Bentain-Melanson, Jesslyn Lenox, Adam R. Smith, Jon C. Boyer, Alison Gassett, Manfred Brigl, Mohamad Sater, Miriam Huntley, Ann E. Woolley, Hilary J. Goldberg, Karen Reilly, Andrew Resnick, Madelyn Pearson, Michael Klompas
Summary: A cluster of 8 Burkholderia cepacia complex infections was associated with contaminated ECMO water heaters, highlighting the potential risks of water-based care for vulnerable patients. This cluster was identified through whole-genome sequencing and environmental sampling, leading to implementation of mitigation measures and removal of water heaters from clinical service.
CLINICAL INFECTIOUS DISEASES
(2022)
Article
Microbiology
Cedric Lood, Charlotte Peeters, Quentin Lamy-Besnier, Jeroen Wagemans, Daniel De Vos, Marijke Proesmans, Jean-Paul Pirnay, Fedoua Echahidi, Denis Pierard, Matthieu Thimmesch, Anca Boeras, Katrien Lagrou, Evelien De Canck, Elke De Wachter, Vera van Noort, Rob Lavigne, Peter Vandamme
Summary: The study found that Burkholderia multivorans, a pathogenic bacterium within the Burkholderia cepacia complex, primarily spreads among cystic fibrosis patients through environmental sources with rare cases of patient-to-patient transmission. Genomic analysis of an endemic strain infecting multiple patients revealed significant genomic diversity between patients, suggesting the presence of a microdiverse reservoir for this strain with active diversification. Additionally, the study identified specific genomic adaptations across multiple patients, indicating that the genomic background of a strain may dictate its route of adaptation within the cystic fibrosis lung.
Article
Multidisciplinary Sciences
Olga Kondratenko, Artem Lyamin, Tatiana Savinova, Yuliya Bocharova, Elena Vasilyeva, Igor Chebotar
Summary: This report describes a pediatric case of paranasal sinusitis caused by Burkholderia cenocepacia in a cystic fibrosis patient. It is an unusual case where the paranasal sinuses were the only site of colonization by B. cenocepacia for five years, with no infection in other organs. The sinuses were sanitized by endoscopic sinus surgery and no B. cenocepacia was detected in samples after the surgery, even without systemic antibiotic therapy for two years. This case demonstrates the possibility of prolonged remission of Bcc-associated paranasal sinusitis in the absence of systemic antibiotics.
Review
Immunology
Silvia A. Sousa, Antonio M. M. Seixas, Joana M. M. Marques, Jorge H. Leitao
Summary: This review discusses immunization and immunotherapy approaches against human infections caused by opportunistic pathogens such as Pseudomonas aeruginosa and Burkholderia cepacia complex. It covers vaccination strategies, ongoing clinical trials, as well as novel bacterial targets for development of immunization and immunotherapy strategies.
Review
Medicine, General & Internal
Freddy Frost, Matthew Shaw, Dilip Nazareth
Summary: This review examined the current evidence for long-term antibiotic therapy in people with CF and chronic BCC infection, finding insufficient evidence to determine an effective strategy for treating chronic BCC infection. The primary objective of the review was to assess the efficacy of treatments in terms of improvements in lung function and reductions in exacerbation rate, with secondary objectives including quantifying adverse events, mortality, and changes in quality of life associated with treatment.
COCHRANE DATABASE OF SYSTEMATIC REVIEWS
(2021)
Article
Infectious Diseases
Frieder Schaumburg, Evgeny A. Idelevich, Alexander Mellmann, Barbara C. Kahl
Summary: Burkholderia cepacia complex (Bcc) in the airways of cystic fibrosis (CF) patients is associated with higher morbidity and mortality. This study assessed the susceptibility of Bcc to ceftazidime/avibactam and other standard drugs used for CF treatment, as well as identified the genomic determinants of resistance. The results showed that Bcc had a high susceptibility rate to ceftazidime/avibactam, while being less susceptible to other antibiotics.
MICROBIAL DRUG RESISTANCE
(2022)
Article
Microbiology
Andrew O'Connor, Irene Jurado-Martin, Margaritha M. M. Mysior, Anotidaishe L. L. Manzira, Joanna Drabinska, Jeremy C. C. Simpson, Mary Lucey, Kirsten Schaffer, Rita Berisio, Siobhan McClean
Summary: Universal stress proteins (USPs) are important for adaptation to environmental conditions and play a key role in chronic cystic fibrosis infection. Among them, USP76 is involved in host cell attachment and survival in CF macrophages.
Article
Biotechnology & Applied Microbiology
Evelise Bach, Fernando Hayashi Sant'Anna, Guilherme Dubal dos Santos Seger, Luciane Maria Pereira Passaglia
Summary: This study performed pangenome analysis on Burkholderia sensu lato for the first time, and focused on the specific features of B. catarinensis in the pangenome of Burkholderia sensu stricto and Burkholderia cepacia complex (Bcc). The results showed great variability and unknown potential in these genomes. The genome of B. catarinensis contains genes related to hydmlase production and plant growth promotion.
Article
Cardiac & Cardiovascular Systems
Davide Tosi, Mario Nosotti, Gianluca Bonitta, Paolo Mendogni, Luca Bertolaccini, Lorenzo Spaggiari, Alex Brunelli, Enrico Ruffini, Pierre Emmanuel Falcoz
Summary: According to the ESTS database, segmentectomy is preferred for 'compromised' patients with limited respiratory function, higher ASA score, and relevant comorbidities, despite a lower rate of postoperative complications, the efficacy is similar to lobectomy.
INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
(2021)
Article
Oncology
C. Basse, A. Botticella, T. J. Molina, P. E. Falcoz, Y. Oulkhouir, M. Kerjouan, E. Pichon, V Westeel, L. Thiberville, X. Quantin, C. Clement-Duchene, J. Khalifa, F. Le Tinier, M. Ginoux, F. Thillays, P. Mordant, B. Besse, P. A. Thomas, C. Le Pechoux, Nicolas Girard
Summary: The RADIORYTHMIC trial aims to compare postoperative radiotherapy with surveillance for patients with localized thymoma, with a primary endpoint of recurrence-free survival. The study will include 314 patients, with a systematic central pathologic review before enrollment and recruitment mainly through a network of 15 expert centers in France. Follow-up will last for 7 years with results expected in 2028.
CLINICAL LUNG CANCER
(2021)
Article
Oncology
Joseph Seitlinger, Mathilde Prieto, Francesco Guerrera, Arthur Streit, Guillaume Gauchotte, Joelle Siat, Pierre-Emmanuel Falcoz, Gilbert Massard, Lorenzo Ferri, Jonathan Spicer, Stephane Renaud
Summary: The prognostic value of NLR and its kinetic evolution in lung cancer patients undergoing surgery remains poorly studied. In this multi-centre retrospective study including 2027 patients, NLR was correlated with overall survival and time to recurrence. Low pre-operative NLR is associated with longer OS in patients with resected NSCLC.
CLINICAL LUNG CANCER
(2022)
Article
Anesthesiology
Nicolas Nesseler, Guillaume Fadel, Alexandre Mansour, Marylou Para, Pierre-Emmanuel Falcoz, Nicolas Mongardon, Alizee Porto, Astrid Bertier, Bruno Levy, Cyril Cadoz, Pierre-Gregoire Guinot, Olivier Fouquet, Jean-Luc Fellahi, Alexandre Ouattara, Julien Guihaire, Vito-Giovanni Ruggieri, Philippe Gaudard, Francois Labaste, Thomas Clavier, Kais Brini, Nicolas Allou, Corentin Lacroix, Juliette Chommeloux, Guillaume Lebreton, Michael A. Matthay, Sophie Provenchere, Erwan Flecher, Andre Vincentelli
Summary: This study reported the characteristics, management, and outcomes of COVID-19 patients receiving extracorporeal membrane oxygenation in France. The in-hospital mortality rate was higher than previous reports, but nearly half of the patients survived. Factors associated with in-hospital mortality included the use of neuromuscular blockade, duration of ventilation, age, and total bilirubin levels at cannulation.
Article
Cardiac & Cardiovascular Systems
Benjamin Bottet, Caroline Rivera, Marcel Dahan, Pierre-Emmanuel Falcoz, Sophie Jaillard, Jean-Marc Baste, Agathe Seguin-Givelet, Richard Bertrand de la Tour, Francois Bellenot, Alain Rind, Dominique Gossot, Pascal-Alexandre Thomas, Xavier Benoit D'Journo
Summary: The majority of reported patient safety incidents (PSIs) in minimally invasive thoracic surgery (MITS) were non-catastrophic, with human factors being the main cause. Systematic reporting and analysis of these incidents can help surgeons and their teams prevent a significant proportion of them.
INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
(2022)
Article
Biochemistry & Molecular Biology
Joseph Seitlinger, Anasse Nounsi, Ysia Idoux-Gillet, Eloy Santos Pujol, Helene Le, Erwan Grandgirard, Anne Olland, Veronique Lindner, Cecile Zaupa, Jean-Marc Balloul, Eric Quemeneur, Gilbert Massard, Pierre-Emmanuel Falcoz, Guoqiang Hua, Nadia Benkirane-Jessel
Summary: Researchers have developed a vascularized patient-derived tumoroid model that preserves histopathological features of tumors and mimics the tumor microenvironment. This model shows promise for personalized medicine and can facilitate more accurate drug screening and treatment testing.
Article
Medicine, General & Internal
Filippo Lococo, Dania Nachira, Marco Chiappetta, Jessica Evangelista, Pierre Emmanuel Falcoz, Enrico Ruffini, Paul Van Schil, Marco Scarci, Jozsef Furak, Francesco Sollitto, Francesco Guerrera, Lorenzo Spaggiari, Clemens Aigner, Liverakou Evangelia, Andrea Bille, Bernhard Moser, Pascal Alexandre Thomas, Moishe Liberman, Souheil Boubia, Alessio Campisi, Luca Ampollini, Alper Toker, Attila Enyed, Luca Voltolini, Dirk Van Raemdonck, Stefano Margaritora
Summary: This study investigated the incidence of myasthenia gravis (MG) and its influence on long-term survival in patients with thymic carcinoma. The results showed that approximately 10% of thymic carcinoma patients had MG, but it did not significantly affect the long-term prognosis in surgically treated patients.
Review
Biochemistry & Molecular Biology
Helene Le, Joseph Seitlinger, Veronique Lindner, Anne Olland, Pierre-Emmanuel Falcoz, Nadia Benkirane-Jessel, Eric Quemeneur
Summary: Synthetic 3D multicellular systems derived from patient tumors, or tumoroids, have been developed to overcome the limitations of current preclinical models and improve the success rate of drug development. Lung cancer research still requires extensive study. This review analyzes the advantages and disadvantages of current preclinical models used in lung cancer research and the role of tumoroids.
Article
Cardiac & Cardiovascular Systems
Michel Gonzalez, Louis-Emmanuel Chriqui, Herbert Decaluwe, Clemens Aigner, Ferenc Renyi-Vamos, Isabelle Opitz, Jozsef Furak, Zalan Szanto, Alessandro Brunelli, Pierre-Emmanuel Falcoz
Summary: This study based on the European Society of Thoracic Surgeons database provides insights into the surgical practices and perioperative outcomes of sleeve resections. The results show that sleeve lobectomy is a safe and effective surgical approach for centrally located lung tumors.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2022)
Article
Health Care Sciences & Services
Marco Alifano, Elisa Daffre, Laurent Brouchet, Pierre Emmanuel Falcoz, Francoise Le Pimpec Barthes, Pierre Benoit Pages, Pascal Alexandre Thomas, Marcel Dahan, Raphael Porcher
Summary: In this study, a simple and easy-to-use scoring system was developed using clinical data to predict the 5-year overall survival in patients with resectable non-small cell lung cancer. The scoring system showed good predictive accuracy and could provide personalized predictions based on disease stage.
LANCET REGIONAL HEALTH-EUROPE
(2023)
Article
Cardiac & Cardiovascular Systems
Luca Bertolaccini, Pierre-Emmanuel Falcoz, Alessandro Brunelli, Hasan Batirel, Jozsef Furak, Stefano Passani, Zalan Szanto
Summary: The GDPR, implemented in 2018 in the European Union, has had a significant impact on personal data management and protection. It applies to all entities collecting and processing personal data of EU residents, regardless of their location, and emphasizes meticulous handling and protection of identifiable information. The regulation sets legal principles for data processing, emphasizes accountability, and has influenced data management practices, such as in the European Society of Thoracic Surgeons (ESTS) database.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2023)
Article
Cardiac & Cardiovascular Systems
Charlotte Ponte, Omar Alkhatiri, Anne Olland, Pierre-Emmanuel Falcoz
Summary: A best-evidence topic was constructed and written according to a structured protocol to address the question of whether lungs from donors above 60 years old have equivalent outcomes in lung transplant patients. Out of over 200 papers found, 12 provided the best evidence to answer this question. Results varied depending on the analysis of donor age and the recipients' age and initial diagnosis, but overall, lungs from donors above 60 years old can offer comparable results to younger donors when carefully assessed and allocated to specific recipients.
INTERDISCIPLINARY CARDIOVASCULAR AND THORACIC SURGERY
(2023)
Article
Cardiac & Cardiovascular Systems
Tamim Ahmad Haidari, Leizl Joy Nayahangan, Flemming Bjerrum, Henrik Jessen Hansen, Lars Konge, Gilbert Massard, Hasan Fevzi Batirel, Nuria Maria Novoa, Richard Stephen Milton, Rene Horsleben Petersen
Summary: A Delphi survey was conducted to identify and prioritize technical procedures for simulation-based training in the thoracic surgical curriculum. The survey involved 34 key opinion leaders from 14 countries and resulted in the selection of 17 procedures for training.
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
(2023)
Article
Cardiac & Cardiovascular Systems
Laura Filaire, Olaf Mercier, Agathe Seguin-Givelet, Olivier Tiffet, Pierre Emmanuel Falcoz, Pierre Mordant, Pierre-Yves Brichon, Philippe Lacoste, Axel Aubert, Pascal Thomas, Francoise Le Pimpec-Barthes, Ioana Molnar, Magali Vidal, Marc Filaire, Geraud Galvaing
Summary: This study reports on the management of superior vena cava graft infection. The majority of infections were caused by polytetrafluoroethylene grafts, and the treatment consisted of either conservative or surgical strategies. Graft removal was not always necessary for all cases.
INTERACTIVE CARDIOVASCULAR AND THORACIC SURGERY
(2022)