Article
Biochemistry & Molecular Biology
Abigail Taylor, Mark Warner, Christopher Mendoza, Calvin Memmott, Tom LeCheminant, Sara Bailey, Colter Christensen, Julie Keller, Arminda Suli, Dario Mizrachi
Summary: The tight junction (TJ) is a structure composed of multiple proteins responsible for cell-cell adhesion in polarized endothelium and epithelium. This article introduces a new experimental method to study the membrane protein claudins (CLDNs), providing a novel approach for studying other membrane proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Yunika Mayangsari, Mayu Okudaira, Chinatsu Mano, Yuki Tanaka, Osamu Ueda, Tomohiro Sakuta, Yoshiharu Suzuki, Yoshinari Yamamoto, Takuya Suzuki
Summary: Research showed that DMF can enhance intestinal TJ barrier integrity by increasing the expression of occludin and claudin-1, while reducing the expression of claudin-2. This regulation involves the induction of occludin protein translation via mTOR and silencing Cldn2 mRNA via miR-16-5p.
JOURNAL OF FUNCTIONAL FOODS
(2021)
Article
Multidisciplinary Sciences
Siti Muhamad Nur Husna, Che Othman Siti Sarah, Hern-Tze Tina Tan, Norasnieda Md Shukri, Noor Suryani Mohd Ashari, Kah Keng Wong
Summary: Impairment of cell junction molecules contributes to the breakdown of nasal epithelial barrier in allergic rhinitis patients. Reduced expression of OCLN and CLDN7, associated with urban locations and exposure to second-hand smoke, may be key factors in the defect. The study did not find significant associations between TSLP, DSG3 expression and allergic rhinitis.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Christopher Mendoza, Sai Harsha Nagidi, Dario Mizrachi
Summary: The JAM family, members of the immunoglobulin superfamily, each have unique tertiary structures despite similar secondary structures; heterotypic interactions among JAM family members may be greatly favored compared to homotypic interactions; homotypic or heterotypic interactions among JAMs are stronger than that of E-CADs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Physiology
Sumegha Mitra, Yulia Epshtein, Saad Sammani, Hector Quijada, Weiguo Chen, Mounica Bandela, Ankit A. Desai, Joe G. N. Garcia, Jeffrey R. Jacobson
Summary: The research suggests that the deubiquitinating enzyme UCHL1 plays a significant role in regulating lung vascular permeability by altering adherens and tight junction components, highlighting its importance as a mediator of acute lung injury.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Rina Fujiwara-Tani, Shiori Mori, Ruiko Ogata, Rika Sasaki, Ayaka Ikemoto, Shingo Kishi, Masuo Kondoh, Hiroki Kuniyasu
Summary: CLDN4 is a crucial component of tight junctions in epithelial cells and is overexpressed in many epithelial malignancies. Changes in CLDN4 expression are associated with epigenetic factors, inflammation, and growth factor signaling. CLDN4 plays a role in maintaining the tumor microenvironment and acts as a barrier to anticancer drug entry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Ashley Monaco, Ben Ovryn, Josephine Axis, Kurt Amsler
Summary: The paracellular permeability in epithelial cells involves both a Pore Pathway for small ions and solutes, and a Leak Pathway for larger solutes. While the Pore Pathway is better understood, the proteins and mechanism behind the Leak Pathway remain unresolved and controversial. Further research is needed to clarify the molecular basis and regulation of the Leak Pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Hsiao-Chin Chou, Chun-Mei Cheng, Chi-Hwa Yang, Tzu-Yin Lin, Ya-Wen Liu, Tse-Hua Tan, Yi-Rong Chen
Summary: This study revealed the importance of DUSP3 as a regulator of epithelial TJ and its decrease may be implicated in the progression of epithelial cancers.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Fisheries
Chen Feng, Xiaofeng Liu, Niewen Hu, Yiyang Tang, Mengzhe Feng, Zejun Zhou
Summary: The secretory serine protease Ssp1 in Aeromonas hydrophila plays a crucial role in its pathogenicity by increasing intestinal permeability and disrupting tight junctions. The mutant strain delta Ssp1 exhibited reduced virulence, but showed no differences in biofilm formation, swimming motility, and resistance to environmental stress.
FISH & SHELLFISH IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Sofie Van Den Broucke, Jeroen A. J. Vanoirbeek, Rita Derua, Peter H. M. Hoet, Manosij Ghosh
Summary: This study investigated the impact of environmental and occupational irritants on the airway epithelial barrier using an in vitro coculture model. The findings suggest that graphene nanoparticles have a negative effect on the epithelial barrier, possibly due to slight alterations in tight junction organization.
Article
Neurosciences
Astha Shukla, Utkarsh Bhardwaj, Apoorva, Pankaj Seth, Sunit K. Singh
Summary: This study revealed that miRNA-101 decreases the expression of VE-cadherin and claudin-5 in brain endothelial cells exposed to hypoxic conditions, leading to the disruption of endothelial barrier integrity and increased permeability.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Pharmacology & Pharmacy
Joel Brunner, Sakthikumar Ragupathy, Gerrit Borchard
Summary: Research focuses on modulating intercellular tight junctions to enhance drug absorption and bioavailability, with challenges in commercialization. Target-specific permeation enhancers have potential clinical applications.
ADVANCED DRUG DELIVERY REVIEWS
(2021)
Article
Food Science & Technology
Lucas Heils, Martina Schneemann, Ralf Gerhard, Joerg-Dieter Schulzke, Roland Buecker
Summary: The study investigated the impact of Clostridioides difficile binary toxin (CDT) on the intestinal epithelial barrier and found that sublethal concentrations of CDT induced barrier dysfunction with increased permeability and redistribution of tight junction proteins. This suggests that CDT may contribute to diarrhea by compromising the intestinal barrier function.
Article
Multidisciplinary Sciences
John Xiao He Li, Vivian W. Tang, Kingsley A. Boateng, William M. Brieher
Summary: The study reveals that cadherin puncta are actually interdigitated actin microspikes generated by actin polymerization, rather than being formed by myosin pulling on cadherins. These interlocking microspikes expand the cell surface area and increase surface roughness to promote cell adhesion.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Kenta Shigetomi, Yumiko Ono, Kenji Matsuzawa, Junichi Ikenouchi
Summary: It has been found that cholesterol accumulation is essential for the formation of tight junctions, which are crucial for the epithelial barrier. Despite the absence of tight junctions, cholesterol still accumulates normally in the vicinity of the apical junctions. Moreover, a claudin mutant that cannot bind to Zonula Occludens (ZO) proteins can still form tight junction strands. ZO proteins are not only scaffolds for claudins, but also promote the formation of cholesterol-rich membrane domains at apical junctions through their effect on the junctional actomyosin cytoskeleton.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Pathology
Andrei I. Ivanov, Cheryl Young, Kyle Den Beste, Christopher T. Capaldo, Patrick O. Humbert, Patrick Brennwald, Charles A. Parkos, Asma Nusrat
AMERICAN JOURNAL OF PATHOLOGY
(2010)
Article
Cell Biology
Yu-Heng Lai, Jessica Cheng, Dongmei Cheng, Mattie E. Feasel, Kyle D. Beste, Junmin Peng, Asma Nusrat, Carlos S. Moreno
Article
Biochemistry & Molecular Biology
Carl R. Rankin, Roland S. Hilgarth, Giovanna Leoni, Mike Kwon, Kyle A. Den Beste, Charles A. Parkos, Asma Nusrat
JOURNAL OF BIOLOGICAL CHEMISTRY
(2013)
Article
Otorhinolaryngology
G. Aaron Rogers, Kyle Den Beste, Charles A. Parkos, Asma Nusrat, John M. DelGaudio, Sarah K. Wise
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2011)
Article
Otorhinolaryngology
Sarah K. Wise, Kyle A. Den Beste, Elizabeth K. Hoddeson, Charles A. Parkos, Asma Nusrat
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2013)
Article
Otorhinolaryngology
Oswaldo A. Henriquez, Kyle Den Beste, Elizabeth K. Hoddeson, Charles A. Parkos, Asma Nusrat, Sarah K. Wise
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2013)
Article
Otorhinolaryngology
Sarah K. Wise, Adrienne M. Laury, Elizabeth H. Katz, Kyle A. Den Beste, Charles A. Parkos, Asma Nusrat
INTERNATIONAL FORUM OF ALLERGY & RHINOLOGY
(2014)