Review
Chemistry, Multidisciplinary
Yufen Xiao, Zhongmin Tang, Xiangang Huang, Wei Chen, Jun Zhou, Haijun Liu, Chuang Liu, Na Kong, Wei Tao
Summary: This article provides an overview of the significant advancements in emerging mRNA technologies, along with the accompanying chemical designs and principles. Various nanoparticle delivery strategies are discussed, and typical mRNA delivery platforms for various biomedical applications are highlighted. The article also offers insights into the challenges and future development of clinical translation for these mRNA technologies.
CHEMICAL SOCIETY REVIEWS
(2022)
Article
Chemistry, Multidisciplinary
Yu-An Kuo, Cheulhee Jung, Yu-An Chen, Hung-Che Kuo, Oliver S. Zhao, Trung D. Nguyen, James R. Rybarski, Soonwoo Hong, Yuan- Chen, Dennis C. Wylie, John A. Hawkins, Jada N. Walker, Samuel W. J. Shields, Jennifer S. Brodbelt, Jeffrey T. Petty, Ilya J. Finkelstein, Hsin-Chih Yeh
Summary: The study revealed that the nucleobases at positions 7-12 of the 18-nucleotide-long activator are critical to creating bright NCBs, while positions 4-6 and 2-4 are hotspots to generate yellow-orange and red POTs.
ADVANCED MATERIALS
(2022)
Article
Biochemical Research Methods
James Gooch, Sireethorn Tungsirisurp, Hayley Costanzo, Richard Napier, Nunzianda Frascione
Summary: The study selected ssDNA aptamers against human sperm cells using Cell-SELEX and massively parallel sequencing technologies, revealing promising aptamer candidates that selectively bind sperm cells. These aptamers have the potential to increase the efficiency of sexual offence investigations by facilitating sperm detection.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ye-Lim Kwon, Bo Min Kim, Eun Young Lee, Kyoung-Jin Shin
Summary: This study utilized MPS technology to analyze 25 STRs from 350 samples of four populations, revealing that the sequence-based MPS method showed a higher number of alleles and unique alleles in SE33 compared to the length-based CE method.
SCIENTIFIC REPORTS
(2021)
Review
Endocrinology & Metabolism
Aideen M. McInerney-Leo, Emma L. Duncan
Summary: There have been two major eras in gene discovery history, from linkage analysis to massively parallel sequencing (MPS). MPS has greatly accelerated the identification of disease genes, reducing the time needed to determine disease genes from years to weeks.
FRONTIERS IN ENDOCRINOLOGY
(2021)
Article
Genetics & Heredity
Sihan Liu, Yuanyuan Zeng, Chao Wang, Qian Zhang, Meilin Chen, Xiaolu Wang, Lanchen Wang, Yu Lu, Hui Guo, Fengxiao Bu
Summary: This study validated a new gender-inference tool, seGMM, which accurately determines the gender of samples and identifies sex chromosomal abnormalities. The seGMM tool demonstrated high accuracy and outperformed other existing tools in gender inference on different gene panel datasets. Furthermore, seGMM accurately identified sex chromosomal abnormalities through the analysis of sex chromosome marker genes.
FRONTIERS IN GENETICS
(2022)
Article
Energy & Fuels
Juraj Kardos, Timothy Holt, Vincenzo Fazio, Luca Fabietti, Filippo Spazzini, Olaf Schenk
Summary: This study analyzes the computational aspects in massively parallel simulations from the perspective of efficient hardware utilization, presenting a method for efficiently managing and processing computational tasks. A series of numerical experiments demonstrate that the optimized high-throughput computation strategy significantly reduces response times and prevents memory bottlenecks.
SUSTAINABLE ENERGY GRIDS & NETWORKS
(2022)
Article
Business
Joseph Amankwah-Amoah, Yaw A. Debrah, Wantao Yu, Zhibin Lin, Albert Danso, Samuel Adomako
Summary: This issue presents insights from a variety of accepted papers, examining different technology strategies in emerging economies and the potential for learning from other economies to improve access to new technologies and bridge the technology gap. It also proposes key questions and a new research agenda for the broader field of technology policy and business strategy.
TECHNOLOGICAL FORECASTING AND SOCIAL CHANGE
(2021)
Article
Biochemical Research Methods
Irena Zupanic Pajnic, Carlo Previdere, Tomaz Zupanc, Martina Zanon, Paolo Fattorini
Summary: The introduction of PCR-MPS technologies has changed the approach to allelic STR typing in forensics. It is found that PCR-MPS could generate isometric drop-ins (IDIs), which may mislead the operator regarding the number of contributors in a forensic evaluation. The molecular features of IDIs and duplicate tests may be useful tools for managing this new class of artifacts.
Review
Biochemistry & Molecular Biology
Thassia Mayra Telles Carratto, Vitor Matheus Soares Moraes, Tamara Soledad Frontanilla Recalde, Maria Luiza Guimaraes De Oliveira, Celso Teixeira Mendes-Junior
Summary: Massively parallel sequencing (MPS) has revolutionized DNA analysis in forensic genetics, allowing simultaneous analysis of thousands of genetic markers and generating high-resolution data. It has enabled the discovery of variations in core short tandem repeat loci, development of new marker types, and analysis of DNA methylation patterns, mitochondrial DNA, mRNA, and microRNA. MPS technology also empowers the study of metagenomics for individual identification, post-mortem interval estimation, and geolocation inference.
GENETICS AND MOLECULAR BIOLOGY
(2022)
Review
Genetics & Heredity
Erin M. Gorden, Kimberly Sturk-Andreaggi, Charla Marshall
Summary: Hybridization capture technology has gained attention in the forensic field for its ability to process degraded DNA fragments and enrich mitochondrial DNA or nuclear genome polymorphisms through massively parallel sequencing. As the technology advances, hybridization capture becomes increasingly important in analyzing DNA from aged and degraded remains.
FORENSIC SCIENCE INTERNATIONAL-GENETICS
(2021)
Review
Chemistry, Multidisciplinary
Junyun Cheng, Gaole Lin, Tianhao Wang, Yunzhu Wang, Wenbo Guo, Jie Liao, Penghui Yang, Jie Chen, Xin Shao, Xiaoyan Lu, Ling Zhu, Yi Wang, Xiaohui Fan
Summary: CRISPR-based genetic screening is a powerful approach for unbiased functional genomics research. Single-cell CRISPR screening techniques allow the analysis of regulatory networks in complex biological systems at unprecedented resolution. These methods involve perturbing cells en masse using a pooled CRISPR library and high-content phenotyping.
Article
Obstetrics & Gynecology
Songchang Chen, Xuyang Yin, Sijia Zhang, Jun Xia, Ping Liu, Pingyuan Xie, Huijuan Yan, Xinming Liang, Junyu Zhang, Yiyao Chen, Hongjun Fei, Lanlan Zhang, Yuting Hu, Hui Jiang, Ge Lin, Fang Chen, Chenming Xu
Summary: WGS offers a cost-effective and reliable approach for genome-wide haplotyping and PGT for monogenic disorders, aneuploidy, and structural rearrangements without the need for additional family members, showing 100% concordance with traditional PGT methods.
HUMAN REPRODUCTION
(2021)
Review
Genetics & Heredity
Tianqi Lyu, Yuanbin Lin, Kerong Wu, Zhanglei Cao, Qian Zhang, Jianping Zheng
Summary: This article summarizes the application of single-cell sequencing technologies in bladder cancer research, revealing the heterogeneity of bladder tumor cells and the complex ecosystem of the tumor microenvironment. It also uncovers the therapeutic resistance mechanism in bladder cancer and facilitates the detection of urinary-exfoliated tumor cells, providing new insights into the precision medicine of bladder cancer.
FRONTIERS IN GENETICS
(2022)
Article
Genetics & Heredity
Kyleen Elwick, Patrick Rydzak, James M. Robertson
Summary: This project evaluated the prototype PowerSeq (R) 46GY System using donor DNA and casework-type samples. Modifications to the manufacturer's protocol were tested to improve read coverage and sample results. Different library preparation kits were compared, and the PowerSeq kit was found to be more accurate in quantifying library concentration. The TruSeq library kit provided higher coverage and better overall results compared to the KAPA kit.