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Biology
Anastasiia L. Sivkina, Maria G. Karlova, Maria E. Valieva, Laura L. McCullough, Timothy Formosa, Alexey K. Shaytan, Alexey Feofanov, Mikhail P. Kirpichnikov, Olga S. Sokolova, Vasily M. Studitsky
Summary: Sivkina et al. characterized the interaction between S. cerevisiae histone chaperone FACT and the nucleosome core particle. They found that FACT adopts a more open geometry in the presence of Nhp6, and together they unfold nucleosomes. Nhp6 alters the structure of FACT and the properties of nucleosomes to support nucleosome unfolding.
COMMUNICATIONS BIOLOGY
(2022)
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Biochemistry & Molecular Biology
Barbara Safaric, Erika Chacin, Matthias J. Scherr, Lional Rajappa, Christian Gebhardt, Christoph F. Kurat, Thorben Cordes, Karl E. Duderstadt
Summary: Chromosome replication relies on the recruitment of the nucleosome reorganization activity of the histone chaperone FACT, with coordination from the middle and C-terminal domains of Spt16 and Pob3. The N-terminal region plays a critical role in recruitment by the fork protection complex subunit Tof1. These interactions are essential for robust replication in vitro.
NUCLEIC ACIDS RESEARCH
(2022)
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Biochemistry & Molecular Biology
Imon Goswami, Poorva Sandlesh, Aimee Stablewski, Ilya Toshkov, Alfiya F. Safina, Mikhail Magnitov, Jianmin Wang, Katerina Gurova
Summary: This study highlights the importance of nucleosome maintenance during transcription and suggests that the histone chaperone FACT plays a role in preserving chromatin integrity in mammalian adult stem cells.
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Genetics & Heredity
Emily Biernat, Mansi Verma, Chhabi K. Govind
Summary: RSC is an essential ATP-dependent chromatin remodeling complex in Saccharomyces cerevisiae. It regulates nucleosome-depleted regions (NDRs) by sliding flanking nucleosomes away from NDRs. Depletion of RSC leads to nucleosome encroachment in NDRs and transcription initiation defects. The study compared the effects of catalytic-dead Sth1 and rapid depletion of Sth1 on transcription. Rapid depletion of Sth1 reduces recruitment of TBP and Pol II, while the catalytic-dead mutant exhibits a severe reduction in TBP binding but accumulates Pol II in coding regions. The results suggest a role for RSC in transcription elongation and termination processes.
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Biochemistry & Molecular Biology
Xuezheng Wang, Yuantao Tang, Jiawei Xu, He Leng, Guojun Shi, Zaifeng Hu, Jiale Wu, Yuwen Xiu, Jianxun Feng, Qing Li
Summary: This study uncovers a new mechanism for parental histone recycling, revealing the important role of histone chaperone FACT and its N-terminus of the Spt16 subunit in budding yeast. The Spt16-N domain interacts with the replicative helicase MCM2-7, facilitating the formation of a critical ternary complex for efficient parental histone recycling and transfer to the lagging strand.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Cell Biology
Micah J. McCauley, Michael Morse, Nicole Becker, Qi Hu, Maria Victoria Botuyan, Emily Navarrete, Ran Huo, Uma M. Muthurajan, Ioulia Rouzina, Karolin Luger, Georges Mer, James Maher, Mark C. Williams
Summary: The histone chaperone FACT enhances transcription by targeting DNA-protein interactions. This study demonstrates the impact of FACT on nucleosome stability and dynamics, revealing contradictory functions of different FACT subdomains in nucleosome remodeling. These findings provide insights into the catalytic role of key FACT domains in nucleosome disassembly and reassembly.
Review
Biochemistry & Molecular Biology
Shinya Takahata, Yota Murakami
Summary: DNA is stored in a folded state in the nucleus, and only necessary genetic information is extracted. The key to extracting genetic information lies in chromatin ambivalence, which is characterized into low-density euchromatin and high-density heterochromatin. The yeast FACT complex plays a role in both euchromatin and heterochromatin, regulating gene expression and chromatin structures. It interacts with different partners to activate promoter chromatin or induce transcriptional repression, making it an interesting phenomenon.
Article
Biochemistry & Molecular Biology
Olesya I. I. Volokh, Anastasia L. L. Sivkina, Andrey V. V. Moiseenko, Anna V. V. Popinako, Maria G. G. Karlova, Maria E. E. Valieva, Elena Y. Y. Kotova, Mikhail P. P. Kirpichnikov, Timothy Formosa, Vasily M. M. Studitsky, Olga S. S. Sokolova
Summary: In this study, the effects of curaxin CBL0137 on nucleosome unfolding by FACT were analyzed. It was found that curaxin can induce FACT-dependent nucleosome unfolding and trap FACT in the chromatin of cancer cells. The obtained models suggest novel mechanisms of nucleosome unfolding by FACT and c-trapping by curaxins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Cell Biology
Shinya Takahata, Saori Chida, Aoi Ohnuma, Motoyoshi Ando, Takahiro Asanuma, Yota Murakami
Summary: In heterochromatin, Spt16 recruitment is mediated by Pob3 and HP1/Swi6. Pob3 recruits Spt16 through its Spt16 dimerization and PH domains, while HP1/Swi6 recruits Spt16 through physical interaction of the Swi6 CSD and Spt16 peptidase-like domains.
Article
Biochemistry & Molecular Biology
Xuehui Li, Huiyan Li, Qian Jing, Mengxue Wang, Tingting Hu, Li Li, Qiuping Zhang, Mengxin Liu, Yu Vincent Fu, Junhong Han, Dan Su
Summary: hSSRP1 is an essential component of the facilitates chromatin transcription complex, with multiple functions such as nuclear localization, histone chaperone activity, DNA binding, and interaction with cellular proteins. The study provides insights into the structural basis for hSSRP1 functionality.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Haruhiko Ehara, Tomoya Kujirai, Mikako Shirouzu, Hitoshi Kurumizaka, Shun-ichi Sekine
Summary: This study used cryo-electron microscopy to obtain structures of RNA polymerase II (RNAPII) passing through nucleosomes during gene transcription. The researchers also identified the roles of transcription elongation factors and histone chaperones in nucleosome disassembly and reassembly.
Article
Biochemistry & Molecular Biology
Migle Tomkuvien, Markus Meier, Diana Ikasalaite, Julia Wildenauer, Visvaldas Kairys, Saulius Klimasauskas, Laura Manelyt
Summary: Methylation of cytosine is an important epigenetic mark that can alter DNA and chromatin structure. This study investigates how larger chemical variations in DNA affect chromatin structure and nucleosome formation.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biology
David C. Klein, Santana M. Lardo, Kurtis N. McCannell, Sarah J. Hainer
Summary: The FACT complex maintains cellular pluripotency by regulating nucleosome positioning and transcription activity. FACT interacts with genes such as OCT4, SOX2, and NANOG, binding to both promoter and enhancer elements, and regulates their transcription and expression, thereby influencing cell fate decisions.
Article
Biochemistry & Molecular Biology
Linxuan Zhao, Pengwei Xing, Vamsi Krishna Polavarapu, Miao Zhao, Blanca Valero-Martinez, Yonglong Dang, Nagaprathyusha Maturi, Lucy Mathot, Ines Neves, Irem Yildirim, Fredrik Johansson Swartling, Tobias Sjoblom, Lene Uhrbom, Xingqi Chen
Summary: The study introduces a new method (FACT-seq) for efficient analysis of histone modifications in archived FFPE tissues, significantly improving resolution. High-quality chromatin profiles can be generated using a small number of FFPE nuclei, offering insights into epigenetic regulation in cancer and human disease.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Cell Biology
Magdalena Murawska, R. A. Greenstein, Tamas Schauer, Karl C. F. Olsen, Henry Ng, Andreas G. Ladurner, Bassem Al-Sady, Sigurd Braun
Summary: The formation of heterochromatin involves nucleation, self-propagation, and maintenance steps, with the histone chaperone FACT playing a crucial role in promoting heterochromatin spreading by repressing heterochromatic histone turnover in fission yeast. This study sheds light on the mechanisms underlying the involvement of FACT in heterochromatin silencing.
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Cell Biology
Sarah J. Hainer, Weifeng Gu, Benjamin R. Carone, Benjamin D. Landry, Oliver J. Rando, Craig C. Mello, Thomas G. Fazzio
GENES & DEVELOPMENT
(2015)
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Cell Biology
Sarah J. Hainer, Thomas G. Fazzio
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Genetics & Heredity
Sarah J. Hainer, Joseph A. Martens
EPIGENETICS & CHROMATIN
(2016)
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Biology
Sarah J. Hainer, Kurtis N. McConnell, Jun Yu, Ly-Sha Ee, Lihua J. Zhu, Oliver J. Rando, Thomas G. Fazzio
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Cell Biology
Diwash Acharya, Sarah J. Hainer, Yeonsoo Yoon, Feng Wang, Ingolf Bach, Jaime A. Rivera-Perez, Thomas G. Fazzio
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Biochemistry & Molecular Biology
Sarah J. Hainer, Ana Boskovic, Kurtis N. McCannell, Oliver J. Rando, Thomas G. Fazzio
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Biochemistry & Molecular Biology
David C. Klein, Sarah J. Hainer
CHROMOSOME RESEARCH
(2020)
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Biochemistry & Molecular Biology
Cristina Tavera-Montanez, Sarah J. Hainer, Daniella Cangussu, Shellaina J. V. Gordon, Yao Xiao, Pablo Reyes-Gutierrez, Anthony N. Imbalzano, Juan G. Navea, Thomas G. Fazzio, Teresita Padilla-Benavides
Article
Biochemistry & Molecular Biology
Sarah J. Hainer, Craig D. Kaplan
Review
Biology
Benjamin J. Patty, Sarah J. Hainer
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Biochemistry & Molecular Biology
Sarvenaz Sarabipour, Sarah J. Hainer, Emily Furlong, Nafisa M. Jadavji, Charlotte M. de Winde, Natalia Bielczyk, Aparna P. Shah
Summary: This article provides guidance to researchers on how to write effective recommendation letters, including tips on structure, supporting information, and avoiding biases.
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Biochemistry & Molecular Biology
Sarvenaz Sarabipour, Sarah J. Hainer, Feyza Nur Arslan, Charlotte M. de Winde, Emily Furlong, Natalia Bielczyk, Nafisa M. Jadavji, Aparna P. Shah, Sejal Davla
Summary: Mentorship plays a crucial role in influencing and improving research, well-being, and career development. Positive mentee-mentor relationships are essential for maintaining work-life balance and achieving success in careers. Selecting multiple and diverse mentors, setting goals, and maintaining constructive relationships with mentors are key practices for successful mentorship.
Article
Biochemical Research Methods
Benjamin J. Patty, Sarah J. Hainer
Summary: The uliCUT&RUN protocol allows for profiling of chromatin-associated proteins in low-input samples, enabling the investigation into the functions of DNA-binding proteins in rare samples. This approach provides a new way to assess protein localization with high precision, even in the presence of limited cell numbers.
Article
Multidisciplinary Sciences
Santana M. Lardo, Sarah J. Hainer
Summary: Determining the binding locations of a protein on chromatin is crucial for understanding its function. Chromatin Immunoprecipitation (ChIP) has been the gold standard for over 30 years, but CUT&RUN, a more sensitive antibody tethering technique, is gaining popularity. CUT&RUN is a powerful method for determining protein binding profiles, especially in low cell populations and single cells.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Biology
David C. Klein, Santana M. Lardo, Kurtis N. McCannell, Sarah J. Hainer
Summary: The FACT complex maintains cellular pluripotency by regulating nucleosome positioning and transcription activity. FACT interacts with genes such as OCT4, SOX2, and NANOG, binding to both promoter and enhancer elements, and regulates their transcription and expression, thereby influencing cell fate decisions.