4.6 Article

Short-term effects of excessive anaerobic reaction time on anaerobic metabolism of denitrifying polyphosphate-accumulating organisms linked to phosphorus removal and N2O production

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HIGHER EDUCATION PRESS
DOI: 10.1007/s11783-013-0505-4

关键词

Denitrifying phosphorus removal; anaerobic reaction time; nitrous oxide; polyhydroxyalkanoate; free nitrous acid; fluorescence in-situ hybridization

资金

  1. National Natural Science Foundation of China [51078283, 51178325]
  2. Shanghai Science and Technology Committee Rising-Star Tracking Program [12QH1402400]
  3. Fundamental Research Funds for the Central University (Tongji University)

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The short-term effect of anaerobic reaction time (AnRT) (i.e., 90, 120 and 150 min) on the denitrifying phosphorus (P) removal performance and N2O production was examined using a denitrifying enhanced biologic phosphorus removal (EBPR) sludge acclimatized with mixed acetate (HAc) and propionate (Pro) (in the molar ratio 3:1) as carbon sources. The results showed that when the AnRT was prolonged from 90 to 150 min, the anaerobic polyhydroxyalkanoate (PHA) synthesis was decreased by 15.3%. Moreover, the ineffective PHA consumption occurred in anaerobic phases and contributed to an increased NO (2) (-) -N accumulation and higher free nitrous acid (FNA) concentrations (a (c) 3/40.001-0.0011 mg HNO2-N/L) in the subsequent anoxic phases, causing a severe inhibition on anoxic P-uptake and denitrification. Accordingly, the total nitrogen (TN) and total phosphorus (TP) removal efficiencies dropped by approximately 6.3% and 85.5%, respectively; and the ratio of anoxic N2O-N production to TN removal increased by approximately 3.8%. The fluorescence in situ hybridization (FISH) analysis revealed that the sludge was mainly dominated by Accumulibacter (62.0% (SEmean = 1.5%)). In conclusion, the short-term excessive anaerobic reaction time negatively impacted denitrifying P removal performance and stimulated more N2O production, and its effect on P removal was more obvious than that on nitrogen removal.

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