Article
Biotechnology & Applied Microbiology
Ziwan Ji, Zhongming Cai, Shuming Gu, Yucang He, Zikai Zhang, Tian Li, Qing Wei, Jingping Wang, Chen Ke, Liqun Li
Summary: The study found that adipose-derived exosomes (ADEs) can inhibit adipogenic differentiation of adipose-derived stem cells by activating the Hedgehog (Hh) signaling pathway, thereby reducing the adverse effects of obesity on wound healing.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Physical
Chunhui Sheng, Xiaodong Guo, Zhuqing Wan, Xiaoqiang Bai, Hao Liu, Xiao Zhang, Ping Zhang, Yunsong Liu, Wenyue Li, Yongsheng Zhou, Longwei Lv
Summary: This study investigated the potential of exosomes derived from human adipose-derived stem cells (hASCs) in promoting osteogenic differentiation for osteoporosis treatment. The study identified the key differentially expressed microRNA between two exosomes and their potential target gene. Results showed that exosomes from osteogenic induction medium had a better effect on promoting osteogenic differentiation than those from proliferation medium. Moreover, miR-335-3p inhibitor-optimized exosomes enhanced osteogenic differentiation in vitro and bone density in vivo, suggesting the potential of engineered exosomes in optimizing exosome-based therapy for osteoporosis.
Article
Biochemistry & Molecular Biology
Szu-Hsien Wu, Yu-Ting Liao, Chi-Han Huang, Yi-Chou Chen, En-Rung Chiang, Jung-Pan Wang
Summary: ADSCs tend to spontaneously differentiate into neuron-like cells during high confluence culture, with enhanced expression of neuronal markers and neurotrophins, while BMSCs do not show this tendency. Additionally, ADSCs do not exhibit spontaneous adipogenic and osteogenic differentiation during this process.
Article
Biochemistry & Molecular Biology
Enrico C. Torre, Mesude Bicer, Graeme S. Cottrell, Darius Widera, Francesco Tamagnini
Summary: Adipose-derived mesenchymal stromal cells (ASCs) have the potential to differentiate into various cell types and exhibit spontaneous Ca2+ oscillations. The effects of differentiation treatments on ASCs' Ca2+ dynamics, in terms of oscillation fraction and frequency, were examined in this study. Both adipogenic and osteogenic differentiation resulted in reduced Ca2+ dynamics, with adipogenic differentiation showing a more pronounced effect. Changes in Ca2+ oscillations were observed as early as 7 days in vitro (DIV). Further research is needed to understand the molecular and mechanistic correlates of these changes for the development of interventions in regenerative medicine.
Review
Biochemistry & Molecular Biology
Chiara Mazziotta, Carmen Lanzillotti, Maria Rosa Iaquinta, Francesca Taraballi, Elena Torreggiani, John Charles Rotondo, Lucia Oton-Gonzalez, Elisa Mazzoni, Francesca Frontini, Ilaria Bononi, Monica De Mattei, Mauro Tognon, Fernanda Martini
Summary: MSCs are important source for bone regeneration and play a crucial role in osteogenic differentiation. MiRNAs regulate gene expression in osteogenic differentiation of MSCs and are involved in controlling important signaling pathways such as TGF-beta/BMP and Wnt/beta-catenin.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nan Zhou, Zhao Xu, Xiang Li, Sen Ren, Jing Chen, Hewei Xiong, Cheng Wang, Jiahe Guo, Yu Kang, Zhenbing Chen, Wenqing Li, Xiaofan Yang, Xing Zhang, Xiang Xu
Summary: This study aimed to induce the differentiation of ADSCs into SCs using SC-Exos and investigate the mechanisms involved through miRNA sequencing. The results showed that SC-Exos were effective in inducing ADSCs differentiation, with differentially expressed miRNAs playing a vital role.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Kang-Hsi Wu, Ju-Pi Li, Wan-Ru Chao, Yi-Ju Lee, Shun-Fa Yang, Ching-Chang Cheng, Yu-Hua Chao
Summary: The study found that human umbilical cord-derived MSCs effectively attenuated the cytokine storm and lung injury in mice after LPS-induced ALI by down-regulating the MyD88-NFκB signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Bo Jia, Jun Chen, Qin Wang, Xiang Sun, Jiusong Han, Fernando Guastaldi, Shijian Xiang, Qingsong Ye, Yan He
Summary: The study reveals that SIRT6 physically interacts with DNMT1 protein, deacetylating and destabilizing it, leading to the activation of NOTCH1 and NOTCH2, which promotes the osteogenic differentiation of ADSCs.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Baojun Sun, Yajie Sun, Shuwei Han, Ruitong Zhang, Xiujuan Wang, Chunxia Meng, Tuo Ji, Chunhui Sun, Na Ren, Shaohua Ge, Hong Liu, Yang Yu, Jingang Wang
Summary: Traditional bone defect treatments face limitations due to insufficient supply of autologous bone, immune rejection of allogeneic bone grafts, and high medical costs. To overcome these limitations, bone tissue engineering with electroactive scaffolds has emerged as a promising option. However, current electroactive scaffolds are either single-function and not suitable for scientific research and clinical application, or complex and expensive to prepare. This study proposes a new tissue engineering strategy to optimize the electrical properties and biocompatibility of carbon-based materials.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hiroshi Kanno, Shutaro Matsumoto, Tetsuya Yoshizumi, Kimihiro Nakahara, Masamichi Shinonaga, Atsuhiko Kubo, Satoshi Fujii, Yasuyuki Ishizuka, Masaki Tanaka, Masamitsu Ichihashi, Hidetoshi Murata
Summary: Adipose-derived mesenchymal stem cells (ADMSCs) can differentiate into various cell types, including cholinergic neurons, by using a peptide derived from SOCS7 protein. This novel method has potential applications in research and regenerative medicine for cholinergic neuron diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Cadenas-Martin Marta, Moratilla Adrian, Fernandez-Delgado Jorge, Arnalich-Montiel Francisco, Maria P. De Miguel
Summary: Corneal disease affects millions worldwide and standard treatment involves transplantation, but there is a shortage. Lamellar endothelial keratoplasty and directed differentiation of adult stem cells show promise in addressing the issue. Efforts are ongoing to find new sources of corneal endothelial cells for transplantation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biotechnology & Applied Microbiology
Zilong Zhang, Jin Shang, Qinyan Yang, Zonglin Dai, Yuxin Liang, Chunyou Lai, Tianhang Feng, Deyuan Zhong, Haibo Zou, Lelin Sun, Yuhao Su, Su Yan, Jie Chen, Yutong Yao, Ying Shi, Xiaolun Huang
Summary: This study suggests that exosomes derived from human adipose mesenchymal stem cells (hADMSCs-Exo) have significant therapeutic effects on liver fibrosis and cirrhosis. The researchers found that hADMSCs-Exo can inhibit the proliferation of activated hepatic stellate cells, effectively suppress the expression of profibrogenic proteins and epithelial-to-mesenchymal transition (EMT), and improve liver function and reduce liver inflammation. The key mechanism of hADMSCs-Exo in anti-hepatic fibrosis is the inhibition of the PI3K/AKT/mTOR signaling pathway and the regulation of choline metabolism.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Cell & Tissue Engineering
Shuanglin Peng, Sirong Shi, Gang Tao, Yanjing Li, Dexuan Xiao, Lang Wang, Qing He, Xiaoxiao Cai, Jingang Xiao
Summary: This study identified that decreased JKAMP expression in DOP-ASCs inhibited osteogenic ability by suppressing the Wnt signaling pathway. The overexpression of JKAMP can enhance the osteogenic capacity of DOP-ASCs, indicating that intragenic DNA methylation regions may be one of the reasons for the reduced osteogenic ability of DOP-ASCs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Agriculture, Dairy & Animal Science
Ejaz R. Dar, Mudasir B. Gugjoo, Moien Javaid, Shahid Hussain, Mujeeb R. Fazili, Kuldeep Dhama, Taha Alqahtani, Ali M. Alqahtani, Riaz A. Shah, Talha Bin Emran
Summary: The study evaluates the influence of ambient temperature, donor physiological status, and tissue source on sheep mesenchymal stem cells. Results show that adipose tissue-derived MSCs from full term pregnant female sheep exhibit higher potential for proliferation and differentiation compared to male sheep-derived MSCs. Cryopreserved MSCs have similar characteristics to fresh cells. Therefore, it is important to consider tissue type and donor physiological status when applying MSCs in clinical settings.
Article
Biotechnology & Applied Microbiology
Luminita Labusca, Dumitru-Daniel Herea, Anca Emanuela Minuti, Cristina Stavila, Camelia Danceanu, Petru Plamadeala, Horia Chiriac, Nicoleta Lupu
Summary: Iron oxide based magnetic nanoparticles (MNP) have versatile applications in biology and medicine. This study compared the chondrogenic conversion of ADSC-MNP and WJMSC under different conditions, finding that ADSC-MNP displayed superior proliferation and chondrogenic potential, with MF exposure further enhancing chondrogenesis in ADSC-MNP. Loading ADSC with MNP may be a promising approach for cartilage engineering in the future.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Yuting Wen, Mei Zhang, Yangxue Yao, Yang Gao, Xiaolin Zhang, Yunfeng Lin, Xiaoxiao Cai
Summary: The hyperplasia and destruction of synovial tissue have a significant impact on the development of rheumatoid arthritis. Targeting anomalous synovial fibroblast and designing a high bioavailability nano drug delivery system can reduce the dosage for the treatment of rheumatoid arthritis and improve curative effect. The experiment established a nobiletin-loaded tetrahedral framework nucleic acids cargo tank which effectively alleviated the disease development of rheumatoid arthritis by reducing the invasiveness of pathological synovial fibroblast.
CHINESE CHEMICAL LETTERS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Songhang Li, Yuxuan Zhao, Taoran Tian, Tianxu Zhang, Yu Xie, Xiaoxiao Cai
Summary: The use of resorbable sutures for fixing a titanium mesh can achieve similar results to traditional fixation using titanium screws. Although this new fixation method can improve the efficiency of the surgery and reduce the risk of complications, the long-term clinical effects require further follow-up investigation.
CLINICAL IMPLANT DENTISTRY AND RELATED RESEARCH
(2023)
Review
Engineering, Biomedical
Yang Yao, Yuhuan Jiang, Jialu Song, Ruojing Wang, Zhaoping Li, Lei Yang, Weimin Wu, Luyue Zhang, Qiang Peng
Summary: This review provides an overview of exosomes and their applications in tissue regeneration. It summarizes the approaches used to modify exosomes for enhanced targeting capacity and discusses current concerns and future prospects of exosomes in tissue engineering.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Cell Biology
Yanan Lin, Yueying Jiang, Haiyu Xian, Xiaoxiao Cai, Tao Wang
Summary: In this research, the expression of the Pi3k/Akt pathway and VEGF in OSF were investigated, and their correlation was explored. It was found that the expression of Col-I increased as OSF progressed, but was downregulated in normal and moderate to advanced OSF tissues. VEGF expression correlated positively with Pi3k and Akt expression, and targeted regulation of the Pi3k/Akt pathway can induce VEGF expression and improve ischemia, ultimately treating OSF.
CELL PROLIFERATION
(2023)
Article
Biochemistry & Molecular Biology
Yuanhao Dong, Jianhong Liu, Yuan Chen, Tao Zhu, Yuanhong Li, Chaoliang Zhang, Xin Zeng, Qianming Chen, Qiang Peng
Summary: By loading MXene nanomaterial into non-crosslinked chitosan hydrogel, MX-CS hydrogel was developed to effectively treat MRSA infections through the adsorption of MRSA cells and MXene-induced photothermal therapy.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Yangxue Yao, Xiaoyu Lei, Yun Wang, Geru Zhang, Hongxiao Huang, Yuxuan Zhao, Sirong Shi, Yang Gao, Xiaoxiao Cai, Shaojingya Gao, Yunfeng Lin
Summary: Diabetic peripheral neuropathy (DPN) is a major late complication of diabetes, and dysfunctional mitochondria are the key therapeutic target. In this study, a tetrahedral framework nucleic acid carrier was used to encapsulate resveratrol, resulting in enhanced stability and bioavailability. The tFNAs-RSV complex showed promising therapeutic effects in vitro and in mice models of DPN by regulating redox and energy metabolism.
Article
Chemistry, Multidisciplinary
Yu Xie, Jiajun He, Songhang Li, Xingyu Chen, Tianxu Zhang, Yuxuan Zhao, Yunfeng Lin, Xiaoxiao Cai
Summary: A transdermal drug delivery system, composed of tetrahedral framework nucleic acids (tFNAs) and lipoic acid (LA), is developed for the topical treatment of skin photodamage. The tFNAs-LA (TLA) nanocomposite exhibits excellent biocompatibility and various therapeutic effects. In both in vitro and in vivo experiments, TLA promotes tissue healing, inhibits apoptosis activation and reactive oxygen species production, and regulates inflammatory responses.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Chemistry, Applied
Yao Lin, Jingchen Xu, Yuanhao Dong, Yue Wang, Chenhao Yu, Yuanhong Li, Chaoliang Zhang, Qianming Chen, Song Chen, Qiang Peng
Summary: We developed a drug-free and non-crosslinked chitosan (CS)/hyaluronic acid (HA) hydrogel with multiple functions for synergistic healing of MRSA-infected diabetic wounds. The CS/HA hydrogel exhibited broad-spectrum antibacterial activity, promoted fibroblast proliferation and migration, scavenged reactive oxygen species (ROS), and protected cells under oxidative stress. In MRSA-infected diabetic mouse wounds, CS/HA hydrogel significantly promoted wound healing by eliminating MRSA infection and enhancing epidermal regeneration, collagen deposition, and angiogenesis. With its drug-free feature, ready availability, excellent biocompatibility, and remarkable wound healing efficacy, CS/HA hydrogel shows great potential for managing chronic diabetic wounds in clinical use.
CARBOHYDRATE POLYMERS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Yang Gao, Shaojingya Gao, Yangxue Yao, Xiaoxiao Cai
Summary: This study evaluated the radiographic stability of bone substitute augmented outside the buccal bony arch contour in the maxillary esthetic zone. The results showed that the overaugmented bone tended to remodel into the original contour, highlighting the importance of careful observation of the anterior bone arch contour for determining implant position and bone augmentation width.
CLINICAL ORAL IMPLANTS RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Xuelin Tang, Jianhong Liu, Ruijiao Yan, Qiang Peng
Summary: Eyes are vulnerable to infections, causing various ocular disorders. This article summarizes the applications of carbohydrate bioadhesive polymers (CBPs) in treating ocular diseases, such as chitosan, hyaluronic acid, cellulose, cyclodextrin, alginate, and pectin. It provides a comprehensive understanding of CBP-based formulations for ocular use, discussing patents, clinical trials, concerns, and possible solutions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Shaojingya Gao, Yao Jiang, Yangxue Yao, Songhang Li, Xiaoxiao Cai
Summary: This study aimed to introduce a minimally invasive technique for maxillary sinus floor elevation using the lateral approach (lSFE) and determine factors influencing stability of grafted area in sinus cavity. Thirty patients (30 implants) treated with lSFE using minimally invasive techniques from 2015 to 2019 were included. CBCT was used to measure bone heights (BHs) of implants before surgery, immediately after surgery (T0), 6 months after surgery (T1), and at last follow-up visit (T2). No implant failed during the 3.67±1.75 years of follow-up. Three implants exhibited perforations. BH changes showed strong correlations with each other and decreased before second-stage surgery. RBH did not significantly influence BH changes, while smoking status and type of bone graft materials were potentially influential factors. In conclusion, lSFE using minimally invasive techniques was a viable treatment option with high implant survival rate and limited bone resorption in grafted area.
INTERNATIONAL JOURNAL OF ORAL SCIENCE
(2023)
Article
Pediatrics
Tian Tian, Tongjia Gan, Jun Chen, Jun Lu, Guiling Zhang, Yiran Zhou, Jia Li, Haoyue Shao, Yufei Liu, Hongquan Zhu, Di Wu, Chengcheng Jiang, Jianbo Shao, Jingjing Shi, Wenzhong Yang, Wenzhen Zhu
Summary: An intelligent HIE identification model (DLCRN) based on conventional structural MRI and clinical characteristics was developed and validated in this study. The DLCRN model showed good discrimination, calibration, and clinical applicability in diagnosing HIE. Visualization of the DLCRN highlighted the lesion areas that conformed to radiological identification. Scientific application of the optimized DLCRN model may save time for screening early mild HIE, improve the consistency of HIE diagnosis, and guide timely clinical management.
Review
Materials Science, Biomaterials
Ruijiao Yan, Jianhong Liu, Zaiquan Dong, Qiang Peng
Summary: Oral diseases, such as dental caries, periodontitis and oral cancer, are commonly caused by bacterial infections or malignant cell proliferation. Photodynamic therapy (PDT) is an effective treatment for these diseases as it can be easily applied to superficial lesions and has antimicrobial and anticancer properties. However, the use of photosensitizers (PSs) in PDT is limited by their low solubility and stability. Recent advances in nanomedicine-based PDT have addressed these limitations by utilizing functionalized nanomaterials as carriers or direct PSs, thereby enhancing the efficacy of PDT. This review provides a comprehensive understanding of different oral diseases and discusses the potential applications of nanomedicine-based PDT in their treatment, as well as the concerns and possible solutions for nanomaterials-mediated PDT.
BIOMATERIALS ADVANCES
(2023)
Article
Engineering, Biomedical
Jingchen Xu, Yao Lin, Yue Wang, Hongyu Gao, Yuanhong Li, Chaoliang Zhang, Qianming Chen, Song Chen, Qiang Peng
Summary: Developing multifunctional wound dressings accommodating the complex microenvironment of diabetic wounds is of great significance. In this study, a multifunctional hydrogel loaded with regeneration silicon (Regesi-CS) showed great antibacterial activity, improved cell function, and promoted wound healing in diabetic wound models.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Chemistry, Physical
Geyun Chen, Zhenghao Yang, Xiaotong Yu, Chenhao Yu, Shangyan Sui, Chaoliang Zhang, Chongyun Bao, Xin Zeng, Qianming Chen, Qiang Peng
Summary: In this study, a multifunctional nanomaterial photo-thermal agent, AGO, was developed for anti-oral cancer photothermal therapy. AGO has a positive charge and significantly enhanced photothermal effect, allowing it to efficiently interact with tumor cells and retain in tumor tissue. The enhanced photothermal effect enables AGO to achieve tunable and efficient photothermal therapy.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
