Article
Biochemistry & Molecular Biology
Saeed Arshad, Mengli Wei, Qurban Ali, Ghulam Mustafa, Zhengqiang Ma, Yuanxin Yan
Summary: This study reveals the advanced usage of anticancer drugs Paclitaxel and Caffeine-Taurine for doubling maize haploids. Compared to the traditional colchicine, Paclitaxel and Caffeine-Taurine are better alternatives in improving chromosome-doubling rates in maize crop.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
MohammadMehdi Habibi, Musavvara Kh Shukurova, Kazuo N. Watanabe
Summary: The research aimed to provide a reference process for Zingiberaceae species reproduced with vegetative propagation, optimizing chromosome doubling for tetraploid production. The highest percentage of tetraploid plants was obtained with colchicine treatment for Hedychium gardnerianum, while oryzalin treatment resulted in mixoploid plants for H. coronarium. The results indicated that in vitro polyploid induction is optimized with the two Hedychium species, potentially applicable to other Zingiberaceae species.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
(2022)
Article
Biotechnology & Applied Microbiology
Xiqing Zhang, Jiangyun Gao
Summary: This study reports a novel in vitro colchicine-induced chromosome doubling protocol that effectively converts diploid D. cariniferum protocorms into tetraploids, with 120 tetraploid genotypes identified by flow cytometry. Tetraploids displayed obvious morphological and anatomical variations compared to diploids, as well as a 1.75-fold greater genome size.
PLANT CELL TISSUE AND ORGAN CULTURE
(2021)
Article
Plant Sciences
Huijing Wu, Lanlan Jiang, Jin'e Li, Min Lu, Huaming An
Summary: This study successfully induced polyploidy in Rosa roxburghii f. eseiosa through colchicine treatment and tissue culture, providing a foundation for its breeding and development.
Article
Agronomy
Raquel M. de O. Pires, Edila V. de R. V. Pinho, Genaina A. Souza, Danielle R. Vilela, Heloisa O. dos Santos, Wilson V. S. Pereira
Summary: This study investigates the use of anatomical analysis tools as indirect markers for ploidy identification in maize plants. The results indicate that the anatomical tool can be used as a marker for differentiating ploidy in maize plants and can be applied in selection programs.
Article
Plant Sciences
Joe Abdul Vilcherrez-Atoche, Jessica Coutinho Silva, Wellington Ronildo Clarindo, Mateus Mondin, Jean Carlos Cardoso
Summary: This study aimed to develop a protocol for polyploidizing Blc. orchids by testing different explants (seeds and protocorms), concentrations, and exposure times to colchicine. The use of protocorms treated with colchicine resulted in the highest percentage of polyploid plants (16.4%), with the best outcome observed at a colchicine concentration of 500-750 mu M for 18 hours. Natural polyploidization in vitro using protocorms was also reported (11.5%). Cytological analysis allowed for the estimation of chromosome numbers in the parents, polyploidized progeny, and non-polyploidized progeny.
Article
Agronomy
Sahar Hussein Hamarashid, Yasin Khaledian, Faranak Soleimani
Summary: Polyploidy induction is an effective method to increase the phyto-metabolites content in medicinal plants. In Lamb's-ear, treatment with colchicine may result in tetraploidy, leading to stronger growth and higher levels of secondary metabolites.
GENETIC RESOURCES AND CROP EVOLUTION
(2022)
Article
Horticulture
Yang Liu, Shan-De Duan, Yin Jia, Li-Hong Hao, Di-Ying Xiang, Duan-Fen Chen, Shan-Ce Niu
Summary: Wild D. officinale is endangered, while cultivated D. officinale has inferior quality and low medicinal components. In this study, tetraploids of D. officinale were successfully generated using colchicine treatment. Morphological observations showed that tetraploids had smaller size, different leaf shape, and larger stem diameter. Cytological observations revealed larger stomata and fewer stomata per unit area in polyploid plants. Chromosomal observations showed that treatment with 0.2% colchicine for 48 hours resulted in the highest tetraploid induction rate.
Article
Plant Sciences
Yanfen Gao, Junchao Ma, Jiaqi Chen, Qian Xu, Yanxia Jia, Hongying Chen, Weiqi Li, Liang Lin
Summary: This study successfully established a novel method for establishing homogeneous tetraploid embryogenic cell lines in Magnolia officinalis, maximizing the production of solid polyploids through regenerants of single cell origin, and confirming the stability of ploidy level through flow cytometry and chromosome counting.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Nuray Comlekcioglu
Summary: Anther culture experiment on pepper showed that adding colchicine significantly increased embryo frequency, and chromosome doubling was necessary to restore diploid status and fertility.
PAKISTAN JOURNAL OF BOTANY
(2021)
Review
Cell Biology
Mathew Bloomfield, Daniela Cimini
Summary: An increase in centrosome number is commonly observed in cancer cells, but its role and occurrence during cancer development remains unclear. Whole genome doubling (WGD) can generate cancer cells with extra centrosomes, and it is associated with poor survival in over 30% of human cancers. Recent findings suggest that newly formed tetraploid cells in vitro lose their extra centrosomes to prevent multipolar cell divisions, raising the possibility that it may be advantageous for tetraploid cells to initially restore centrosome number homeostasis and reacquire additional centrosomes in the later stages of cancer evolution.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Razieh Kiani, Ahmad Arzani, S. A. M. Mirmohammady Maibody, Mehdi Rahimmalek, Tina Ayers
Summary: By hybridizing wheat genotypes with the salt-tolerant genotype Aegilops cylindrica, amphidiploid plants with enhanced salt tolerance were produced. These amphidiploids showed distinct characteristics in morphology and phylogenetic relationships, and exhibited better growth performance under salt stress conditions compared to wheat cultivars.
JOURNAL OF PLANT BIOCHEMISTRY AND BIOTECHNOLOGY
(2021)
Article
Horticulture
Krishna Bhattarai, Abdul Kareem, Zhanao Deng
Summary: Cultivated gerbera is an important flower worldwide and a model plant for studying flower development and secondary metabolites biosynthesis. Inducing tetraploids in gerbera resulted in larger flowers, thicker leaves, wider ray florets, and higher tolerance to stresses, which could be beneficial for breeding and increasing genetic diversity.
SCIENTIA HORTICULTURAE
(2021)
Article
Agriculture, Multidisciplinary
H. Razmi, R. Amiri Fahliani, B. Kavoosi, A. Masoumiasl
Summary: This study induced polyploidy in strawberries using colchicine and found that a concentration of 0.7% was the most effective. The tetraploid plants showed larger fruit size and lower stomata number compared to the diploid plants.
JOURNAL OF AGRICULTURAL SCIENCE AND TECHNOLOGY
(2022)
Article
Horticulture
Nasrin Sabooni, Ali Gharaghani, Abolfazl Jowkar, Saeid Eshghi
Summary: This research successfully generated polyploid blackberry plants using in vitro method, and found that different species reacted differently to polyploidy induction. Morphometric analysis of leaves showed that polyploidy affected guard cells, cell size, and cell count. This in vitro procedure could be a promising tool for creating blackberry cultivars with enhanced fruit characteristics and possible resistance to environmental stress.
SCIENTIA HORTICULTURAE
(2022)
Article
Agronomy
Michele Carla Nadal, Gustavo Magno dos Reis Ferreira, Gracielle Vidal Silva Andrade, Victor Hugo Buttros, Filipe Almendagna Rodrigues, Caroline Marcela da Silva, Adalvan Daniel Martins, Leo Rufato, Jose Magno Queiroz Luz, Joyce Doria, Moacir Pasqual
Summary: This study investigated the presence of beneficial microorganisms in Pyrus communis rootstocks through isolation and identification, selecting auxin-producing bacteria. Inoculation of selected microorganisms showed promotion of growth and root emission in vitro. This study opens the door for further research using new and more promising microbial isolates, providing a cheaper alternative to synthetic auxin in vegetative propagation of woody fruit trees.
Article
Agronomy
Rafael Hansen Madail, Leila Aparecida Sales Pio, Renata Alves Lara Silva Rezende, Moacir Pasqual, Sebastiao de Oliveira e Silva
Summary: This study evaluated the leaf anatomical characteristics of banana cultivars and found that leaf thickness, stomatal size, and density can be used as appropriate parameters for characterizing banana ploidy levels.
ACTA SCIENTIARUM-AGRONOMY
(2022)
Article
Plant Sciences
Ana Livia Martins Scarpa, Filipe Almendagna Rodrigues, Yasmini da Cunha Cruz, Vinicius Politi Duarte, Evaristo Mauro de Castro, Moacir Pasqual, Fabricio Jose Pereira
Summary: The objective of this study was to test the tolerance of two species of Schinus and two species of Handroanthus cultivated in iron mining tailings from the rupture of the dam in Mariana, Brazil. The results showed that the mining tailings contained beneficial and potentially toxic elements, and seeds and seedlings were still able to germinate and survive in the tailings. Different plants had different responses to the tailings, with Schinus species showing higher tolerance.
SEED SCIENCE RESEARCH
(2022)
Article
Plant Sciences
Ana Livia Martins Scarpa, Yasmini da Cunha Cruz, Vinicius Politi Duarte, Evaristo Mauro de Castro, Moacir Pasqual, Jean Paulo Vitor de Oliveira, Fabricio Jose Pereira
Summary: This study aimed to assess the effects of nutrient solution on the ecophysiological characteristics of Handroanthus serratifolius and Handroanthus impetiginosus cultivated in iron mine tailings. The results showed that adding nutrient solution increased the dry mass and photosynthesis of H. impetiginosus, but decreased stomatal conductance in H. serratifolius.
JOURNAL OF SOIL SCIENCE AND PLANT NUTRITION
(2022)
Article
Agronomy
Ronilson Carlos Araujo, Mariana Souza Ribeiro, Filipe Almendagna Rodrigues, Bruna Santos Silva, Joyce Doria, Moacir Pasqual
Summary: Banana micropropagation with endophytic growth-promoting bacteria and hydroponic system allows for the quick production of high-quality seedlings, reducing the production cycle and improving plant growth and development.
ARCHIVES OF AGRONOMY AND SOIL SCIENCE
(2023)
Article
Environmental Sciences
Leticia A. Bressanin, Andre A. M. Diniz, Kamila R. D. de Souza, Ligiane A. Florentino, Adriano B. da Silva, Paulo C. Magalha, Moacir Pasqual, Thiago C. de Souza
Summary: One of the largest accidents with mine tailings occurred in Brazil in 2015. In this study, the researchers investigated the growth and physiology of the tree species Hymenaea courbaril in mine tailings. They hypothesized that inoculation with diazotrophs would improve the growth and promote reforestation. The results showed that Hymenaea courbaril has the ability to survive in mine tailings, and inoculation with diazotrophic bacteria improved its physiological aspects. The strain UNIFENAS100-638 was found to be the most effective in promoting the growth of the plants.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2022)
Article
Horticulture
Gracielle Vidal Silva Andrade, Filipe Almendagna Rodrigues, Michele Carla Nadal, Caroline Marcela da Silva Dambroz, Adalvan Daniel Martins, Vantuil Antonio Rodrigues, Gustavo Magno dos Reis Ferreira, Moacir Pasqual, Victor Hugo Buttros, Joyce Doria
Summary: This study isolated endophytic bacteria from root and leaf microbiomes in different Cattleya walkeriana orchid agroecosystems (natural habitat, greenhouse, and in vitro cultivation) to explore the mechanisms promoting plant and enzymatic growth. A total of 67 endophytic bacteria were isolated, all of which showed biological nitrogen fixation capacity. Some strains also demonstrated the ability to produce plant growth-promoting substances and solubilize nutrients. These bacterial strains have the potential to serve as bioinoculants for sustainable orchid cultivation.
SCIENTIA HORTICULTURAE
(2023)
Article
Agronomy
Maysa Mathias Alves Pereira, Ludmila Caproni Moraes, Maria Cristina Troya Mogollon, Carlos Junio Falconi Borja, Mateus Duarte, Victor Hugo Teixeira Buttros, Jose Magno Queiroz Luz, Moacir Pasqual, Joyce Doria
Summary: Based on the concepts of circular economy and bioeconomy, the reuse of agrifood residues through vermicomposting can help solve environmental problems and contribute to sustainable production systems. The study focused on identifying, quantifying and analyzing the physical, chemical, hormonal, amino acid content and microbial biodiversity of different vermicompost formulations. The results showed that the vermicomposts met the minimum requirements for organic fertilizer and had differences in parameters such as phosphorus, auxin, tryptophan, organic matter, and humic and fulvic acids. Inoculation with microorganisms from native forest promoted biodiversity and reduced heavy metal levels in the compost. Vermicomposting is concluded to be a potential tool in reusing agri-food residues and maintaining soil biodiversity and fertility.
Article
Food Science & Technology
Suelen S. S. Gaspar, Lucas L. R. Assis, Carla A. A. Carvalho, Victor Hugo Buttros, Gustavo Magno dos Reis Ferreira, Rosane F. F. Schwan, Moacir Pasqual, Filipe Almendagna Rodrigues, Everlon Cid Rigobelo, Rafael Peron Castro, Joyce Doria
Summary: Organic wastes are a significant part of urban solid waste and have a negative impact on the environment. Composting is a sustainable method for managing organic waste, and this study evaluates the efficiency of a new composter and the microbial and physiochemical dynamics during composting. The results show that the new composter and the use of inoculants can accelerate the degradation of organic waste and produce high-quality compost.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2022)
Article
Food Science & Technology
Suelen da Silva Gaspar, Lucas Lenin Resende de Assis, Maria Paula Ramos do Prado, Maria Gabriela Pedroso Miguel, Gustavo Magno dos Reis Ferreira, Rosane Freitas Schwan, Moacir Pasqual, Everlon Cid Rigobelo, Rafael Peron Castro, Victor Hugo Buttros, Joyce Doria
Summary: This study analyzed and identified the biodiversity of yeasts and filamentous fungi throughout a composting process. The results showed that the treatments with commercial and non-commercial inoculum had higher microbial activity, diversity, and efficiency in the transformation of organic matter, leading to better quality of the final product compared to the control treatment.
FRONTIERS IN SUSTAINABLE FOOD SYSTEMS
(2023)
Article
Integrative & Complementary Medicine
Fernando Simoni Bacilieri, Ana Valeria Vieira de Souza, Jose Magno Queiroz Luz, Roberta Camargos de Oliveira, Herick Fernando de Jesus Silva, Renata Alves L. Silva Rezende, Moacir Pasqual, Joyce Doria
Summary: This study evaluated the effects of different plant growth regulators and ethylene inhibitors on the development and leaf abscission of Schinopsis brasiliensis Engl. Results showed that the combination of specific concentrations of indolacetic acid (IAA) and zeatin (ZEA) promoted the growth of new shoots in Schinopsis brasiliensis. Cobalt chloride was more effective than silver nitrate in reducing leaf abscission. The combination of IAA and ZEA was found to be the most suitable for increasing shoot length and number of buds. Silver nitrate completely prevented leaf abscission at a concentration of 20 mu M, while cobalt chloride had the maximum reduction effect at a concentration of 40 mu M.
BOLETIN LATINOAMERICANO Y DEL CARIBE DE PLANTAS MEDICINALES Y AROMATICAS
(2023)
Article
Agronomy
Ellem W. N. Contado, Moacir Pasqual, Joyce Doria, Rolando J. Gonzalez-Pena, Lionel X. Dupuy, Roberto A. Braga Jr
Summary: Dynamic laser speckle (DLS) analysis is a sensitive technique used to measure biological activity. The use of infrared (IR) lasers in DLS systems improves the stability of measurements in seed germination applications. The results show that IR light reduces the variability of the measurements and is less sensitive to pigments, allowing for better penetration into samples and correlation with biological processes. These findings support the wider use of IR lasers in DLS-based instruments for biological samples.
Review
Agronomy
Leandro Israel da Silva, Marlon Correa Pereira, Andre Mundstock Xavier de Carvalho, Victor Hugo Buttros, Moacir Pasqual, Joyce Doria
Summary: Phosphorus is essential for plant growth, but the nutrients provided by fertilizers become inert in the medium term and cannot be assimilated by plants. The use of phosphorus-solubilizing microorganisms is a viable option for optimizing phosphorus use.
Article
Agronomy
Michele Carla Nadal, Otavio Bernardes Machado, Ronilson Carlos Araujo, Filipe Almendagna Rodrigues, Leo Rufato, Joyce Doria, Moacir Pasqual
Summary: This study evaluated the use of 6-Benzylaminopurine (IBA) in in vitro rooting of Pyrus comunnis rootstocks, clones 'OHxF87' and Pyrodwarf. The results showed that a 24-hour treatment at 20 mg L-1 IBA was sufficient to promote in vitro rooting in both 'OHxF87' and Pyrodwarf rootstocks, with high survival rates and significant root formation.
Article
Multidisciplinary Sciences
Renata A. Moreira, Mariane A. Rodrigues, Rafaela C. De Souza, Alexandre D. Da Silva, Fabio O. R. Silva, Carolina G. Lima, Leila A. S. Pio, Moacir Pasqual
Summary: This study evaluated the viability of self-pollination, cross-pollination, and natural pollination in pitaya flowers, as well as the pollen viability, stigma receptivity, and ideal pollination window. The results showed that artificial pollination of white-fleshed pitaya is viable and results in larger fruits compared to natural pollination, with cross-pollination being the most recommended method. The flowers of pitaya have receptive stigma throughout the floral opening period, and the highest pollen germination rate is observed at 7 p.m., which is the ideal pollination window for pitaya.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
