Article
Oncology
Hyunsoo Cho, Kyung Hwan Kim, Hoyoung Lee, Chang Gon Kim, Haerim Chung, Yoon Seok Choi, Su-Hyung Park, June-Won Cheong, Yoo Hong Min, Eui-Cheol Shin, Jin Seok Kim
Summary: By studying NK cells in patients with multiple myeloma, it was found that adaptive NK cells have lower levels of CD38 expression compared to conventional NK cells, and exhibit stronger anti-tumor activity in daratumumab-mediated effector functions. The composition of adaptive NK cells in the bone marrow of patients with multiple myeloma determines their ex vivo functional activity in response to daratumumab.
CLINICAL CANCER RESEARCH
(2021)
Article
Immunology
Alistair Noble, Basudev Paudyal, John C. Schwartz, William Mwangi, Danish Munir, Elma Tchilian, John A. Hammond, Simon P. Graham
Summary: Cattle possess three IgG subclasses with different functionalities, which differ from those described in humans, mice, or pigs. This has important implications for the development of vaccines and therapeutic antibodies.
FRONTIERS IN IMMUNOLOGY
(2023)
Review
Biotechnology & Applied Microbiology
Mikel Garcia-Alija, Berre van Moer, Diego E. Sastre, Tala Azzam, Jonathan J. Du, Beatriz Trastoy, Nico Callewaert, Eric J. Sundberg, Marcelo E. Guerin
Summary: Antibody-based drugs, including IgG monoclonal antibodies, are widely used for treating cancer, autoimmune, and infectious diseases. Different glycoforms attached to antibodies can affect their efficacy, stability, and effector functions. Therefore, various antibody engineering strategies have been developed to produce antibodies with tailored glycoforms.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Immunology
Jeremiah L. Oyer, Tayler J. Croom-Perez, Thomas A. Dieffenthaller, Liza D. Robles-Carillo, Sarah B. Gitto, Deborah A. Altomare, Alicja J. Copik
Summary: This study evaluated the impact of cryopreservation on PM21-NK cells and found that PM21-NK cells can be efficiently cryopreserved and recovered without substantial loss of viability or activity, while retaining effector function both in vitro and in vivo.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Vidya S. S. Shivatare, Po-Kai Chuang, Tzu-Hao Tseng, Yi-Fang Zeng, Han-Wen Huang, Gannedi Veeranjaneyulu, Han-Chung Wu, Chi-Huey Wong
Summary: A comprehensive structure-activity relationship study on antibody Fc-glycosylation was conducted using a chimeric anti-SSEA4 antibody chMC813-70 as a model. The α-2,6 sialylated biantennary complex type glycan was identified as the optimal Fc-glycan, which significantly enhanced antibody effector functions, including binding to different Fc receptors and ADCC.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Yannic C. Bartsch, Deniz Cizmeci, Jaewon Kang, Tomer Zohar, Sivakumar Periasamy, Nickita Mehta, Jeroen Tolboom, Leslie Van der Fits, Jerry Sadoff, Christy Comeaux, Benoit Callendret, Alexander Bukreyev, Douglas A. Lauffenburger, Arangassery Rosemary Bastian, Galit Alter
Summary: RSV infection is a major cause of severe respiratory tract infection and death in infants and the elderly. This study found that protection against RSV infection is linked to opsonophagocytic functions driven by IgA and differentially glycosylated RSV-specific IgG profiles, indicating the importance of functional humoral immune response in preventing RSV.
Review
Immunology
Andrea Cottignies-Calamarte, Daniela Tudor, Morgane Bomsel
Summary: Recent advances in therapeutic antibodies have greatly improved the treatment of drug-resistant cancers and autoimmune diseases. Fc-bioengineering has been widely used to enhance the efficacy and convenience of therapeutic antibodies, although IgG remains the most commercially available. However, interest in IgA is growing due to its enhanced binding to Fc receptors and potential synergistic effects with IgG.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Immunology
Zixi Wang, Di Guan, Jianxin Huo, Subhra K. Biswas, Yuhan Huang, Yuansheng Yang, Shengli Xu, Kong-Peng Lam
Summary: The study demonstrates that IL-10 upregulates both glycolysis and oxidative phosphorylation in human NK cells, crucial for their enhanced effector functions. Mechanistically, it is revealed that IL-10 activates the mammalian target of rapamycin complex 1 (mTORC1) to regulate metabolic reprogramming in human NK cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Beatrice Charreau
Summary: The injury of graft endothelial cells by donor-specific antibodies is a key factor in antibody-mediated rejection. Various effector mechanisms involving NK cells and macrophages are important contributors to this rejection process, in addition to the activation of the classical complement pathway by DSA IgG. NK cells can also be involved in rejection by killing graft endothelial cells.
Article
Virology
Amanda B. Macedo, Callie Levinger, Bryan N. Nguyen, Jonathan Richard, Mamta Gupta, Conrad Russell Y. Cruz, Andres Finzi, Katherine B. Chiappinelli, Keith A. Crandall, Alberto Bosque
Summary: In this study, we found that HODHBt enhances IL-15 signaling in natural killer cells, leading to increased cytotoxicity and memory-like functions. This suggests that pharmacological enhancement of IL-15-mediated STAT activation could be a new approach for HIV treatment.
JOURNAL OF VIROLOGY
(2022)
Review
Biochemistry & Molecular Biology
Sytse J. Piersma, Ilija Brizic
Summary: Natural killer (NK) cells are innate lymphoid cells that play a crucial role in controlling tumors and viral infections by producing cytokines and lysing target cells. The activation of NK cells depends on signals received by cytokine receptors and activation and inhibitory receptors recognizing ligands expressed by virus-infected cells. The focus of ongoing research has been on understanding the signals perceived by NK cells and how they integrate to mediate optimal viral control.
Article
Multidisciplinary Sciences
Ian Wilkinson, Stephen Anderson, Jeremy Fry, Louis Alex Julien, David Neville, Omar Qureshi, Gary Watts, Geoff Hale
Summary: Novel variants with specific amino acid substitutions at L234 and L235 combined with G236R substitution completely eliminate binding to Fc. receptors, avoiding inflammatory responses and improving the safety and efficacy of therapeutic antibodies and Fc fusion proteins.
Review
Biotechnology & Applied Microbiology
Simone C. Oostindie, Greg A. Lazar, Janine Schuurman, Paul W. H. Parren
Summary: This review discusses the role of avidity in eliciting antibody functional responses and current engineering strategies for manipulating avidity interactions in antibody-based therapies. Avidity, derived from multiple affinity interactions, is fundamental to all aspects of antibody biology and is crucial for enhancing or engineering novel properties in antibody biotherapeutics. Therapeutic antibody mechanisms of action are comprehensively reviewed with an emphasis on engineered optimizations and platforms, leading to a new wave of differentiated antibody drugs with tailored properties and promising improved treatment options for various diseases.
NATURE REVIEWS DRUG DISCOVERY
(2022)
Article
Immunology
Christin Friedrich, Renske L. R. E. Taggenbrock, Remi Doucet-Ladeveze, Gosia Golda, Rebekka Moenius, Panagiota Arampatzi, Natasja A. M. Kragten, Katharina Kreymborg, Mercedes Gomez de Agueero, Wolfgang Kastenmueller, Antoine-Emmanuel Saliba, Dominic Gruen, Klaas P. J. M. van Gisbergen, Georg Gasteiger
Summary: ILC1 cells exhibit similar differentiation pathways in different organs, with a gradual loss of TCF-1 resulting in loss of proliferative potential and increased expression of effector molecules.
Article
Immunology
Monika Horvat Mercnik, Carolin Schliefsteiner, Herbert Fluhr, Christian Wadsack
Summary: Hofbauer cells (HBCs) of the human placenta play a role in immune tolerance and tissue homeostasis. Preeclampsia (PE) is associated with a switch in the polarization and phenotype of HBCs, with early-onset (EO) and late-onset (LO) PE showing different patterns. This study provides insights into the functionality and plasticity of HBCs in resolving inflammation and maintaining tissue homeostasis.
FRONTIERS IN IMMUNOLOGY
(2023)