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Multidisciplinary Sciences
R. Venkat Kalyana Sundaram, Atrouli Chatterjee, Manindra Bera, Kirill Grushin, Aniruddha Panda, Feng Li, Jeff Coleman, Seong Lee, Sathish Ramakrishnan, Andreas M. Ernst, Kallol Gupta, James E. Rothman, Shyam S. Krishnakumar
Summary: In this study, the full functional reconstitution of genetically validated core protein machinery was used to investigate the fate of synaptic vesicles before and after release. The researchers discovered new roles for diacylglycerol (DAG) in regulating vesicle priming and Ca2+-triggered release involving the SNARE assembly chaperone Munc13.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Cell Biology
Guoyi Yang, Liuju Li, Yanmei Liu, Kuo Liang, Lisi Wei, Liangyi Chen
Summary: Impaired insulin release in type 2 diabetes is closely related to chronically elevated glucose concentrations. This study found that high glucose delays fusion events, reduces full fusion, and decreases the size of fusion pores, all contributing to compromised insulin secretion.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Lucas Bayones, Samuel Alfonso-Bueno, Mauricio Montenegro, Jose Moya-Diaz, Octavio Caspe, Luciana Gallo, Fernando D. Marengo
Summary: This study investigated the endocytosis and replenishment of the immediately releasable pool (IRP) in mice primary chromaffin cells. The researchers found that not only a fast dynamin-dependent endocytosis occurred after the release of about 50% IRP, but also a fast dynamin-independent endocytosis was activated after depolarizations lasting 25-50ms, which released over 80% of IRP. This dynamin-independent endocytosis was found to be Ca2+- and protein kinase C (PKC)-dependent. Additionally, PKC inhibition also affected the slow component of replenishment observed after total IRP exocytosis.
JOURNAL OF NEUROCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Najeeb Ullah, Ezzouhra El Maaiden, Md Sahab Uddin, Ghulam Md Ashraf
Summary: This review focuses on the role of the Syt-1 protein in secretory vesicle docking, priming, and fusion. It highlights how Syt-1 acts as a Ca2+ sensor and participates in forming the docking complex to facilitate vesicle fusion with the plasma membrane.
CURRENT PROTEIN & PEPTIDE SCIENCE
(2021)
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Biochemistry & Molecular Biology
Furong Liu, Ruyue He, Min Zhu, Lin Zhou, Yinghui Liu, Haijia Yu
Summary: In this study, the researchers found that Munc18c protein facilitates SNARE-dependent membrane fusion through an important peptide fragment called SLP. This discovery suggests that the t-SNARE-SLP binding mode might be a conserved mechanism for the stimulatory function of SM proteins in vesicle fusion.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Marco van Tilburg, Peter A. J. Hilbers, Albert J. Markvoort
Summary: Membrane fusion is widely observed in natural and artificial systems, and depends on various protein characteristics. In this study, molecular dynamics simulations were used to examine the effects of these characteristics on the fusion process. It was found that the type of membrane embedding, rigidity, and transmembrane domain length all play a role in the fusion pathway. Additionally, a single spike with sufficient rigidity and attraction can induce a successful fusion reaction, providing design rules for artificial systems.
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Biology
Christopher A. Norman, Shyam S. Krishnakumar, Yulia Timofeeva, Kirill E. Volynski
Summary: This study uses a computational modelling framework to quantitatively assess how the molecular architecture of the fusion clamp affects synaptic vesicle exocytosis. The results support the "release-of-inhibition" model of fast calcium-activated synaptic vesicle fusion and show that dual binding of synaptotagmin-1 and synaptotagmin-7 to the same SNARE complex enables synergistic regulation of neurotransmitter release kinetics and plasticity. The developed framework provides a powerful tool to test molecular models of calcium-triggered neurotransmitter release in silico.
COMMUNICATIONS BIOLOGY
(2023)
Article
Cell Biology
Masataka Kunii, Yuria Noguchi, Shin-ichiro Yoshimura, Satoshi Kanda, Tomohiko Iwano, Erda Avriyanti, Nur Atik, Takashi Sato, Ken Sato, Masaharu Ogawa, Akihiro Harada
Summary: In developing brain, the SNARE complex composed of SNAP23, VAMP8, and Syntaxin1B plays a crucial role in the formation of apical junctional complexes (AJCs) and radial glial cell (RGC) polarization. Its depletion leads to severe hypoplasia of the neocortex, absence of hippocampus or cerebellum, loss of RGC polarity, and alterations in proliferation, differentiation, and apoptosis. The localization of AJC protein N-cadherin by SNAREs is essential for AJC formation and RGC polarization during brain development.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Li Zhang, Lei Li, Ziqing Wei, Hao Zhou, Haowen Liu, Shen Wang, Yijing Ren, Tiankai Dai, Jiafan Wang, Zhitao Hu, Cong Ma
Summary: This study reveals the crystal structure of the CAPS-1 C2PH module, showing a tight interaction between the C2 and PH domains and enhanced binding to PI(4,5)P2 membrane. A novel PI(4,5)P2-binding site is identified in the C2 domain. Disruption of the C2-PH interaction or the PIP2-binding sites impairs CAPS-1 function in Ca2+-regulated exocytosis.
Review
Food Science & Technology
Kyle S. Gregory, K. Ravi Acharya
Summary: Botulinum neurotoxins (BoNTs) are highly potent neurotoxins that cause neuromuscular paralysis by cleaving SNARE complex proteins. They have high affinity and specificity for neuromuscular junctions. This review focuses on the structural analysis of the cell-binding domain of different BoNT subtypes, providing insights into their functions and potential therapeutic applications.
Article
Multidisciplinary Sciences
Alessandro Moro, Anne van Nifterick, Ruud F. Toonen, Matthijs Verhage
Summary: This study identified dynamins as organizers of dense-core vesicle fusion sites in mammalian neurons, playing a crucial role in the release of neuropeptides and neuromodulators. Genetic or pharmacological inactivation of dynamins strongly impaired dense-core vesicle exocytosis, while synaptic vesicle exocytosis remained unaffected. The study concluded that mammalian dynamins regulate dense-core vesicle fusion sites by modulating the balance between fusogenic and non-fusogenic syntaxin-1, promoting its availability for SNARE complex formation and dense-core vesicle exocytosis.
Article
Biochemistry & Molecular Biology
Minmin Xue, Yuwei Cao, Chun Shen, Wanlin Guo
Summary: Vesicle fusion is crucial for neuronal communication, and computational 'microscopies' such as molecular dynamics simulations have provided significant insights into the complex machinery of neurotransmitter release. These computational advances have contributed greatly to fields like neurology, pharmacology, and pathophysiology. This review highlights the progress made in understanding protein/neurotransmitter-membrane interactions related to presynaptic vesicle-membrane fusion and neurotransmitter release through computational methods.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Cell Biology
Tyler N. Starr, Allison J. Greaney, Adam S. Dingens, Jesse D. Bloom
Summary: The study mapped mutations to the SARS-CoV-2 spike receptor-binding domain that escape binding by certain monoclonal antibodies. These mutations are concentrated in specific lineages of SARS-CoV-2. The authors suggest diversifying the epitopes targeted by antibodies and antibody cocktails to make them more resilient to SARS-CoV-2 antigenic evolution.
CELL REPORTS MEDICINE
(2021)
Article
Multidisciplinary Sciences
Houda Yasmine Ali Moussa, Yongsoo Park
Summary: This study reveals the regulatory mechanism of Ca2+ and PIP2 on the cis and trans-membrane interaction of synaptotagmin-1, using native purified vesicles and plasma membrane-mimicking liposomes.
SCIENTIFIC REPORTS
(2022)
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Plant Sciences
Fangfang Niu, Changyang Ji, Zizhen Liang, Rongfang Guo, Yixuan Chen, Yonglun Zeng, Liwen Jiang
Summary: ADP-ribosylation factor (ARF) family proteins play a central role in regulating vesicular traffic and organelle structures in eukaryotes. This study characterized the function of a unique ARF protein, ARFD1B, in Arabidopsis. It was found that ARFD1B is required for cell plate formation, maintenance of Golgi morphology, and plant growth in Arabidopsis by interacting with COAT PROTEIN COMPLEX I components.
Review
Biochemistry & Molecular Biology
Hsin-Fang Chang, Hawraa Bzeih, Praneeth Chitirala, Keerthana Ravichandran, Marwa Sleiman, Elmar Krause, Ulrike Hahn, Varsha Pattu, Jens Rettig
CELLULAR AND MOLECULAR LIFE SCIENCES
(2017)
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Cell Biology
Hsin-Fang Chang, Stefanie Mannebach, Andreas Beck, Keerthana Ravichandran, Elmar Krause, Katja Frohnweiler, Claudia Fecher-Trost, Claudia Schirra, Varsha Pattu, Veit Flockerzi, Jens Rettig
JOURNAL OF CELL BIOLOGY
(2018)
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Neurosciences
Ali H. Shaib, Angelina Staudt, Ali Harb, Margarete Klose, Ahmed Shaaban, Claudia Schirra, Ralf Mohrmann, Jens Rettig, Ute Becherer
FRONTIERS IN CELLULAR NEUROSCIENCE
(2018)
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Multidisciplinary Sciences
Marie-Christine Klein, Katharina Zimmermann, Stefan Schorr, Martina Landini, Patrick A. W. Klemens, Jacqueline Altensell, Martin Jung, Elmar Krause, Duy Nguyen, Volkhard Helms, Jens Rettig, Claudia Fecher-Trost, Adolfo Cavalie, Markus Hoth, Ivan Bogeski, H. Ekkehard Neuhaus, Richard Zimmermann, Sven Lang, Ilka Haferkamp
NATURE COMMUNICATIONS
(2018)
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Neurosciences
Olga Ratai, Claudia Schirra, Elvin Rajabov, Irene Brunk, Gudrun Ahnert-Hilger, Praneeth Chitirala, Ute Becherer, David R. Stevens, Jens Rettig
JOURNAL OF NEUROSCIENCE
(2019)
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Immunology
Praneeth Chitirala, Keerthana Ravichandran, Donatella Galgano, Marwa Sleiman, Elmar Krause, Yenan T. Bryceson, Jens Rettig
FRONTIERS IN IMMUNOLOGY
(2019)
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Multidisciplinary Sciences
Hao Jiang, Thomas J. Esparza, Terrance T. Kummer, Haining Zhong, Jens Rettig, David L. Brody
SCIENTIFIC REPORTS
(2020)
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Biochemistry & Molecular Biology
Michael Estl, Pascal Blatt, Xuemei Li, Ute Becherer, Hsin-Fang Chang, Jens Rettig, Varsha Pattu
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
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Immunology
Praneeth Chitirala, Keerthana Ravichandran, Claudia Schirra, Hsin-Fang Chang, Elmar Krause, Uli Kazmaier, Marcel A. Lauterbach, Jens Rettig
JOURNAL OF IMMUNOLOGY
(2020)
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Immunology
Marwa Sleiman, David R. Stevens, Praneeth Chitirala, Jens Rettig
FRONTIERS IN IMMUNOLOGY
(2020)
Editorial Material
Cell Biology
Jens Rettig, Cosima T. Baldari
IMMUNOLOGY AND CELL BIOLOGY
(2020)
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Biochemistry & Molecular Biology
Hsin-Fang Chang, Marie-Louise Wirkner, Elmar Krause, Jens Rettig
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
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Biology
Praneeth Chitirala, Hsin-Fang Chang, Paloma Martzloff, Christiane Harenberg, Keerthana Ravichandran, Midhat H. Abdulreda, Per-Olof Berggren, Elmar Krause, Claudia Schirra, Trese Leinders-Zufall, Fritz Benseler, Nils Brose, Jens Rettig
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Immunology
Donatella Galgano, Tayebeh Soheili, Matthias Voss, Lamberto Torralba-Raga, Bianca Tesi, Frank Cichocki, Isabelle Andre, Jens Rettig, Marina Cavazzana, Yenan Bryceson
FRONTIERS IN IMMUNOLOGY
(2020)