Article
Immunology
Rachel E. Crossland, Jean Norden, Sakhila Ghimire, Mateja Kralj Juric, Kim F. Pearce, Clare Lendrem, Matthew Collin, Eva Mischak-Weissinger, Ernst Holler, Hildegard T. Greinix, Anne M. Dickinson
Summary: The study found that miR-155 and miR-146a were upregulated in gastrointestinal and skin tissue samples of aGvHD patients, and their expression levels in serum and urine samples were also associated with the risk of developing aGvHD. These results support the potential of miR-155 and miR-146a as biomarkers for aGvHD, but further investigation is needed to understand their role in generalized inflammation and specific pathophysiology.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yi-Yung Hung, Chen-Kai Chou, Yi-Chien Yang, Hung-Chun Fu, El-Wui Loh, Hong-Yo Kang
Summary: This study found that in patients with major depressive disorder, the expression levels of miR-146a in exosomes were higher, and patients who achieved remission showed lower levels of various microRNAs before treatment and increased levels after treatment. Let-7e, miR-145, and miR-146a showed acceptable discrimination between the remission and non-remission groups.
Article
Multidisciplinary Sciences
Sowmya Gunasekaran, Ramakrishnapillai Vyomakesannair Omkumar
Summary: MicroRNAs, specifically miR-146a and miR-200b, play important physiological roles in neurological disorders such as Alzheimer's disease and schizophrenia. They are involved in regulating learning and memory mechanisms and interact with NMDAR subunits. Overexpression of these miRNAs leads to downregulation of related proteins, resulting in cognitive deficits. In rat models, upregulated levels of these miRNAs indicate their involvement in downregulating NMDAR subunits.
Article
Medicine, Research & Experimental
Jose L. Sanchez-Gloria, Roxana Carbo, Mabel Buelna-Chontal, Horacio Osorio-Alonso, Adrian Henandez-Diazcouder, Rosa Luz de la Fuente-leon, Julio Sandoval, Fausto Sanchez, I. Rubio-Gayosso, Fausto Sanchez-Munoz
Summary: Chronic cold exposure exacerbates MCT-induced pulmonary arterial hypertension in rats, resulting in increased levels of inflammatory markers and miRNAs in plasma EVs and lungs.
Article
Immunology
Mahmoud Mahami-Oskouei, Behrooz Norouzi, Ehsan Ahmadpour, Tohid Kazemi, Adel Spotin, Zahra Alizadeh, Roghayeh Ghorbani Sani, Milad Asadi
Summary: The study compared the expression levels of miR-146a and miR-155 in the plasma of patients with hydatidosis and healthy individuals. The results indicated an increased level of both miR-146a and miR-155 in all hydatidosis patients compared to the control group, with no correlation observed in the level of miR-155 between the two groups. Upregulation of miR-146a and miR-155 in plasma may lead to the development of novel biomarkers for echinococcosis, potentially serving as important biomarkers for determining the severity of hydatidosis.
MICROBIAL PATHOGENESIS
(2021)
Article
Biochemistry & Molecular Biology
Julian Roos, Meike Dahlhaus, Jan-Bernd Funcke, Monika Kustermann, Gudrun Strauss, Daniel Halbgebauer, Elena Boldrin, Karlheinz Holzmann, Peter Moeller, Bernadette M. Trojanowski, Bernd Baumann, Klaus-Michael Debatin, Martin Wabitsch, Pamela Fischer-Posovszky
Summary: The study revealed that miR-146a regulates adipose tissue inflammation by suppressing the NPR3 gene, thereby impacting the development of obesity and insulin resistance.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2021)
Article
Multidisciplinary Sciences
Enas M. Hefzy, Noha A. Hassuna, Olfat G. Shaker, Mohamed Masoud, Tebyan A. Abelhameed, Tarek I. Ahmed, Nada F. Hemeda, Mohammed A. Abdelhakeem, Rania H. Mahmoud
Summary: Genetic variants in miR-155 and miR-146a genes may act as potential risk factors for Chronic HBV in the Egyptian population, highlighting the importance of studying miRNA polymorphisms in disease development.
Article
Immunology
Bo Yang, Ruicheng Yang, Bojie Xu, Jiyang Fu, Xinyi Qu, Liang Li, Menghong Dai, Chen Tan, Huanchun Chen, Xiangru Wang
Summary: E. coli infection induced upregulation of miR-155 and miR-146a in astrocytes, which modulated bacteria-triggered neuroinflammatory responses through negative feedback regulation involving the TLR-mediated NF-kappa B and EGFR-NF-kappa B signaling pathways, thus protecting the central nervous system from further neuroinflammatory damage.
JOURNAL OF NEUROINFLAMMATION
(2021)
Article
Immunology
Seungwha Paik, Kyeong Tae Kim, In Soo Kim, Young Jae Kim, Hyeon Ji Kim, Seunga Choi, Hwa-Jung Kim, Eun-Kyeong Jo
Summary: In this study, researchers found that mycobacterial acyl carrier protein (AcpM) can suppress host cell death and promote bacterial growth by upregulating miRNA-155-5p expression. AcpM inhibits certain signaling pathways and fusion processes, interfering with host autophagy and lysosomal function, thus weakening the host's ability to eliminate Mycobacterium tuberculosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Sarah Brunty, Kristeena Ray Wright, Brenda Mitchell, Nalini Santanam
Summary: Activation of trimethylation of histone 3 lysine 27 (H3K27me3) by EZH2 plays a role in endometriosis, with higher expression of PRC2 complex components like JARID2, miR-155, and FOXP3 noted in patient tissues. MiR-155 affects the PRC2 complex but has minimal impact on JARID2 expression, suggesting alternative pathways may be involved. A novel crosstalk between miR-155, the PRC2 complex, JARID2, and PHF19 has been identified, providing potential epigenetic targets for further study in endometriosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Olfat G. Shaker, Omayma O. Abdelaleem, Nermeen A. Fouad, Naglaa A. Ahmed, Hoda A. Hussein, Enas G. Ibrahem, Abdelrahmaan A. Mohamed, Othman M. Ahmed, Doaa Y. Ali
Summary: This study found that miR-146a (rs2910164) and miR-155 (rs767649) gene polymorphisms are closely related to the development of BD in the Egyptian population and also influence disease severity.
ARCHIVES OF MEDICAL SCIENCE
(2022)
Article
Medicine, Research & Experimental
Shashi Bala, Yuan Zhuang, Prashanth Thevkar Nagesh, Donna Catalano, Adam Zivny, Yanbo Wang, Jun Xie, Guangping Gao, Gyongyi Szabo
Summary: This study investigated the therapeutic effect of miR-155 inhibition in liver fibrosis and found that miR-155 deficiency can significantly reduce liver fibrosis. The study also revealed that miR-155 regulates activation of hepatic stellate cells partly via STAT3 signaling.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2023)
Article
Oncology
Neda K. Dezfuli, Shamila D. Alipoor, Neda Dalil Roofchayee, Sharareh Seyfi, Babak Salimi, Ian M. Adcock, Esmaeil Mortaz
Summary: The study found that miR-146a expression was significantly downregulated in NSCLC patients, while IL-6 and TGF-beta levels were elevated. The frequencies of immune cells CD3+ CTLA-4+ and Treg cells were also higher in patients.
FRONTIERS IN ONCOLOGY
(2021)
Review
Oncology
Katerina Kalkusova, Pavla Taborska, Dmitry Stakheev, Daniel Smrz
Summary: MiR-155 is a significant regulator of immune responses against tumors, particularly in different immune cell populations, and it can impact the process of cancer immunoediting. Its role in cellular communication is also important. MiR-155 targeting is increasingly used in cancer immunotherapy.
Article
Neurosciences
Chu-Chiao Tseng, Shao-Cheng Wang, Yi-Chien Yang, Hung-Chun Fu, Chen-Kai Chou, Hong-Yo Kang, Yi-Yung Hung
Summary: Activated toll-like receptor (TLR) signaling has been well investigated in major depressive disorder (MDD) and several factors including TNFAIP3, TLR4, TNIP2, miR-146a, and miR-155 have been found to play important roles in this pathway. Aberrant histone modification, particularly H3K4me3, has also been implicated in psychiatric disorders. This study aimed to explore the H3K4me3 levels in the promoters of these factors in MDD patients and its changes after antidepressant treatment. The results showed significantly lower H3K4me3 levels in the promoters of TNFAIP3, TLR4, TNIP2, miR-146a, and miR-155 in MDD patients compared to healthy controls. These levels did not significantly change after antidepressant treatment, and there were correlations between H3K4me3 levels in TNIP2 and TLR4 promoters and depression severity.
MOLECULAR NEUROBIOLOGY
(2023)
Review
Parasitology
Dayana C. Farhat, Mohamed-Ali Hakimi
Summary: Toxoplasma gondii is considered a successful parasitic pathogen due to its flexibility in responding to different environments, relying on coordinated developmental abilities. Recent studies have uncovered how master regulators epigenetically regulate its development, highlighting new opportunities for epigenetic reprogramming in the field.
TRENDS IN PARASITOLOGY
(2022)
Article
Microbiology
Taylor G. Glausen, Gabriela L. Carrillo, Richard M. Jin, Jon P. Boyle, Jeroen P. J. Saeij, Elizabeth A. Wohlfert, Michael A. Fox, Ira J. Blader
Summary: Toxoplasmic encephalitis can induce seizures in infected individuals, and the parasite gene GRA15 plays a key role in this process by increasing host IL-1 receptor signaling. Inhibition of IL-1 signaling reduces seizure severity in Toxoplasma-infected mice, revealing a potential therapeutic target for controlling seizures associated with toxoplasmic encephalitis.
Review
Microbiology
Mohamed-Ali Hakimi
Summary: Like many intracellular pathogens, Toxoplasma gondii uses effector proteins to manipulate host cells and promote transmission and persistence. This review summarizes how T. gondii effectors control the fate of infected cells and the outcome of infection through specific mechanisms.
ANNUAL REVIEW OF MICROBIOLOGY
(2022)
Editorial Material
Microbiology
Mohamed-Ali Hakimi
Summary: Toxoplasma gondii weakens host metabolic defenses by trapping mitochondrial proteins.
CELL HOST & MICROBE
(2022)
Article
Pathology
Marie-Pierre Brenier-Pinchart, Denis Filisetti, Sophie Cassaing, Emmanuelle Varlet-Marie, Florence Robert-Gangneux, Laurence Delhaes, Juliette Guitard, Helene Yera, Patrick Bastien, Herve Pelloux, Yvon Sterkers
Summary: This multicenter study assessed the performance of the Toxoplasma RealCycler Universal assay for the diagnosis of toxoplasmosis. The commercial PCR showed high sensitivity and specificity in detecting low parasitic loads, making it suitable for diagnosing different forms of toxoplasmosis.
JOURNAL OF MOLECULAR DIAGNOSTICS
(2022)
Article
Microbiology
Yogavel Manickam, Nipun Malhotra, Siddhartha Mishra, Palak Babbar, Abhishek Dusane, Benoit Laleu, Valeria Bellini, Mohamed-Ali Hakimi, Alexandre Bougdour, Amit Sharma
Summary: This study describes the simultaneous targeting of multiple binding sites of the enzyme prolyl-tRNA synthetase (PRS) in Toxoplasma gondii using two potent inhibitors, halofuginone (HFG) and L95. The inhibitors form a ternary complex with the enzyme, inhibiting its activity and showing additive effects in parasite inhibition assays. This novel approach validates the potential of targeting multiple pockets to inhibit druggable proteins, providing a structural framework for further drug development.
Article
Cell Biology
Christopher Swale, Valeria Bellini, Matthew W. Bowler, Nardella Flore, Marie-Pierre Brenier-Pinchart, Dominique Cannella, Lucid Belmudes, Caroline Mas, Yohann Coute, Fabrice Laurent, Artur Scherf, Alexandre Bougdour, Mohamed-Ali Hakimi
Summary: The Apicomplexa, a phylum of single-celled protozoa, cause severe parasitic diseases in humans and animals. Current therapeutics are limited by their efficacy and resistance. In this study, altiratinib, originally developed for glioblastoma, is identified as a potential drug candidate with broad spectrum activity against apicomplexans. Altiratinib specifically targets the TgPRP4K of T. gondii and inhibits the development of intracellular parasites. The molecular basis of the inhibitory mechanism and species selectivity is further elucidated.
SCIENCE TRANSLATIONAL MEDICINE
(2022)
Article
Microbiology
Arne L. Ten Hoeve, Laurence Braun, Matias E. Rodriguez, Gabriela C. Olivera, Alexandre Bougdour, Lucid Belmudes, Yohann Coute, Jeroen P. J. Saeij, Mohamed-Ali Hakimi, Antonio Barragan
Summary: This study reveals how the intracellular parasite Toxoplasma gondii exploits the chemotactic migration ability of macrophages, highlighting the migratory plasticity of differentiated cells in the mononuclear phagocyte system. The parasite effector GRA28 cooperates with host chromatin remodelers to drive the migration of parasitized macrophages. These findings provide insights into the mechanisms underlying the dissemination of T. gondii and the interaction between the parasite and host cells.
CELL HOST & MICROBE
(2022)
Article
Microbiology
David Arranz-Solis, David Warschkau, Benedikt T. Fabian, Frank Seeber, Jeroen P. J. Saeij
Summary: Toxoplasma gondii oocysts, shed in feces from infected felines, are highly infectious and can survive in the environment for years. The oocysts have a stable and highly infectious nature, but their ability to resist environmental stressors is not fully understood.
Article
Microbiology
Manickam Yogavel, Alexandre Bougdour, Siddhartha Mishra, Nipun Malhotra, Jyoti Chhibber-Goel, Valeria J. Bellini, Karl Harlos, Benoit J. Laleu, Mohamed-Ali Hakimi, Amit J. Sharma
Summary: Researchers have discovered that a compound called PPL can bind to a protein involved in the pathogenic mechanism of the Toxoplasma parasite and kill it. Mutations in the protein structure related to PPL have been identified through genetic mutation and DNA sequencing analysis. Therefore, this study provides a method for enhancing the activity of this compound series.
Review
Cell Biology
Christopher Swale, Mohamed-Ali Hakimi
Summary: The 3'-end processing of mRNA is a co-transcriptional process that involves a complex composed of cleavage and polyadenylation specificity factors (CPSFs) to recognize cis-sequence elements on nascent mRNA and carry out cleavage and polyadenylation reactions. Recent studies have provided a mechanistic understanding of this machinery in yeast or metazoans, and the discovery of small molecule inhibitors of CPSF function has stimulated interest in studying this machinery in Apicomplexa. The CPSF complex integrates a novel reader of the N6-methyladenosine (m6A) in these organisms and bridges m6A metabolism to transcription termination.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
