期刊
CELL REPORTS
卷 9, 期 4, 页码 1528-1537出版社
CELL PRESS
DOI: 10.1016/j.celrep.2014.10.040
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类别
资金
- Bio-X Senior Fellowship
- Siebel Scholars Graduate Fellowship
- NIH Biotechnology Training Grant
- Stanford Graduate Fellowship
- Bio-X Postdoctoral Fellowship
- NSF [DEB-1253650]
- NSF CAREER Award [MCB-1149328]
- Div Of Molecular and Cellular Bioscience
- Direct For Biological Sciences [1149328] Funding Source: National Science Foundation
Diversification of cell size is hypothesized to have occurred through a process of evolutionary optimization, but direct demonstrations of causal relationships between cell geometry and fitness are lacking. Here, we identify a mutation from a laboratory-evolved bacterium that dramatically increases cell size through cytoskeletal perturbation and confers a large fitness advantage. We engineer a library of cytoskeletal mutants of different sizes and show that fitness scales linearly with respect to cell size over a wide physiological range. Quantification of the growth rates of single cells during the exit from stationary phase reveals that transitions between feast-or-famine'' growth regimes are a key determinant of cell-size-dependent fitness effects. We also uncover environments that suppress the fitness advantage of larger cells, indicating that cell-size-dependent fitness effects are subject to both biophysical and metabolic constraints. Together, our results highlight laboratory-based evolution as a powerful framework for studying the quantitative relationships between morphology and fitness.
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