Article
Cell Biology
Valeria Savoca, Julieta Rivosecchi, Alice Gaiatto, Annalisa Rossi, Riccardo Mosca, Irene Gialdini, Lorena Zubovic, Toma Tebaldi, Paolo Macchi, Emilio Cusanelli
Summary: This study reveals that the stability of TERRA transcripts is regulated by the RNA-binding protein associated with lethal yellow mutation (RALY). Depletion of RALY leads to decreased levels of TERRA, impaired localization of TERRA at telomeres, and ultimately telomere damage. Furthermore, TERRA interacts with the poly(A)-binding protein nuclear 1 (PABPN1), suggesting an interplay between RALY and PABPN1 in regulating TERRA stability.
Article
Biochemistry & Molecular Biology
Agata Stepien, Jakub Dolata, Tomasz Gulanicz, Dawid Bielewicz, Mateusz Bajczyk, Dariusz J. Smolinski, Zofia Szweykowska-Kulinska, Artur Jarmolowski
Summary: This study discovered that PRP40 in Arabidopsis regulates the assembly of the microprocessor, playing a crucial role in the correct production of miRNA.
Article
Biology
Sangyoon J. Han, Evgenia Azarova, Austin J. Whitewood, Alexia Bachir, Edgar Guttierrez, Alex Groisman, Alan R. Horwitz, Benjamin T. Goult, Kevin M. Dean, Gaudenz Danuser
Summary: The study reveals the crucial role of concurrent recruitment of talin and vinculin in early integrin-based nascent adhesions, which is essential for the maturation of NAs. Specifically, the function of the R8 domain in talin is identified as important for NA maturation, affecting vinculin binding and recruitment rates.
Article
Multidisciplinary Sciences
Nagham Ghaddar, Yves Corda, Pierre Luciano, Martina Galli, Ylli Doksani, Vincent Geli
Summary: Homologous recombination factors protect nascent DNA during DNA replication, but the role of chromatin in this process is unclear. By studying the bacterial Tus/Ter barrier in S. cerevisiae, researchers found that the Set1C subunit Spp1 is recruited behind the stalled replication fork independently of its interaction with Set1. Spp1's chromatin recruitment depends on its PHD domain's interaction with H3K4me3 parental histones behind the fork, and it prevents the accumulation of ssDNA at the stalled fork by restricting Exo1 access. Deleting SPP1 increases the mutation rate upstream of the barrier, leading to microdeletion accumulation. Spp1 ultimately limits nascent DNA availability to nucleases by limiting fork remodeling.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Zhe Sun, Alexander Yakhnin, Peter C. FitzGerald, Carl E. McIntosh, Mikhail Kashlev
Summary: The study reveals that a large number of E. coli sigma70-dependent pauses, clustered at a 10-20-bp distance from promoters, are regulated by Gre cleavage factors constituting a mechanism for rapid response to changing environmental cues. Transcription factors encoding genes are enriched in these pauses, implying that sigma (70) and Gre proteins regulate transcription in response to changing environmental cues. Transcription by bacterial RNA polymerase is interrupted by pausing events that play diverse regulatory roles.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Xu-Xiang Zhang, Yu-Ming Wang, Yan-Dan Su, Fan Zuo, Bin Wu, Xin Nian
Summary: The study demonstrates that miR-26a regulates insulin-induced adipogenic differentiation of ADSCs by modulating the CDK5/FOXC2 pathway, providing insights into the mechanistic understanding of obesity and type 2 diabetes.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2021)
Article
Physiology
Dov Borovsky, Peter Verhaert, Pierre Rouge, Charles A. Powell, Arnold De Loof
Summary: The study found that late trypsin in Culex quinquefasciatus is regulated by TMOF, with an increase in TMOF concentrations leading to a decrease in trypsin activity and potentially affecting trypsin translation.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Karim Rahimi, Annette C. Fuechtbauer, Fardin Fathi, Seyed Javad Mowla, Ernst-Martin Fuechtbauer
Summary: MiR-302/367 is a significant miRNA cluster that induces and maintains pluripotency. Research on the transcriptional control and processing of the miR-302 host-gene in mice reveals that it is regulated through alternative splicing, polyadenylation, and nuclear export.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Yifan Zhang, Ziyue Chen, Fang Wang, Honghua Sun, Xueliang Zhu, Jianping Ding, Tianlong Zhang
Summary: This study demonstrates that Rab9 utilizes Nde1 as an effector to interact with the dynein motor complex, thereby tethering the late endosomes to the dynein motor for their retrograde transport to the TGN.
Article
Neurosciences
Osmar Vieira Ramires Junior, Tiago Marcon dos Santos, Josiane Silva Silveira, Raissa Leite-Aguiar, Robson Coutinho-Silva, Luiz Eduardo Baggio Savio, Angela T. S. Wyse
Summary: This study evaluated the effects of mild hyperhomocysteinemia on behavior and neurochemical parameters in male rats, and investigated the therapeutic effects of ibuprofen and rivastigmine. The results showed that mild hyperhomocysteinemia caused cognitive deficits and impaired motor coordination, but treatment with ibuprofen and rivastigmine reduced these damages.
MOLECULAR NEUROBIOLOGY
(2022)
Article
Neurosciences
Sean K. Ryan, Matija Zelic, Yingnan Han, Erin Teeple, Luoman Chen, Mahdiar Sadeghi, Srinivas Shankara, Lilu Guo, Cong Li, Fabrizio Pontarelli, Elizabeth H. Jensen, Ashley L. Comer, Dinesh Kumar, Mindy Zhang, Joseph Gans, Bailin Zhang, Jonathan D. Proto, Jacqueline Saleh, James C. Dodge, Virginia Savova, Deepak Rajpal, Dimitry Ofengeim, Timothy R. Hammond
Summary: Iron dysregulation is associated with multiple neurodegenerative diseases, including Parkinson's disease. In this study, it is found that iron-loaded microglia are responsive to iron and susceptible to ferroptosis, a form of cell death that is dependent on iron. Iron overload also induces a shift in the microglial transcriptional state that is similar to the transcriptomic signature found in postmortem brain microglia of Parkinson's disease. The removal of microglia delays iron-induced neurotoxicity, suggesting the contribution of microglial response to neurodegeneration.
NATURE NEUROSCIENCE
(2023)
Article
Engineering, Mechanical
Kesheng Xu, Jean Paul Maidana, Patricio Orio
Summary: The coexistence of electrical and chemical synaptic communication among excitatory cells has been confirmed, but the theoretical understanding of hybrid synaptic connections in various dynamical states of neural networks is not fully studied. A neural network model including electrical and chemical synaptic connections was used to investigate synchronization and firing patterns among excitatory cells, revealing tendencies towards synchronization and the ability to cause various firing patterns by slightly changing the synaptic weights. This study lays a foundation for understanding the computational significance of mixed synapse in neural functions.
NONLINEAR DYNAMICS
(2021)
Article
Neurosciences
Shreya Malhotra, Gokulakrishna Banumurthy, Reagan L. Pennock, Jada H. Vaden, Izumi Sugihara, Linda Overstreet-Wadiche, Jacques Wadiche
Summary: Neurotransmitter spillover, a form of communication not easily predicted by anatomy, is regulated by the patterned expression of the excitatory amino acid transporter 4 in the cerebellum. Through experiments using Aldolase C-Venus knock-in mice, researchers found that heterogeneity in EAAT4 expression is the primary determinant of differential spillover between neuronal microzones. This study highlights how neuronal glutamate transporters limit extrasynaptic transmission in a non-cell-autonomous manner and provides new insights into the functional specialization of cerebellar microzones.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Multidisciplinary Sciences
Shuichi P. Obuchi, Hisashi Kawai, Juan C. Garbalosa, Kazumasa Nishida, Kenji Murakawa
Summary: The study found that environmental temperature affects walking speed, step length, and cadence, with higher values observed in winter compared to summer. Cadence was most strongly correlated with daily maximum temperature in a curvilinear relationship. A decrease in environmental temperature below 30 degrees Celsius was found to increase cadence.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Urmi Bandyopadhyay, Pavlina Todorova, Natalya N. Pavlova, Yuma Tada, Craig B. Thompson, Lydia W. S. Finley, Michael Overholtzer
Summary: This study uncovers the lysosomal retention of leucine under starvation conditions and reveals that stored leucine is utilized in protein synthesis, which is regulated by specific proteins.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)