期刊
CELL REPORTS
卷 5, 期 4, 页码 1095-1107出版社
CELL PRESS
DOI: 10.1016/j.celrep.2013.10.027
关键词
-
类别
资金
- Science Foundation Ireland [07/IN1/B958]
- European Union [512113]
- Health Research Board (Ireland) [PR001/2001]
- IRCSET (EMPOWER)
- Health Research Board [RP2006182N]
- O'Sullivan Charitable Trust, UK
Cells must accurately replicate and segregate their DNA once per cell cycle in order to successfully transmit genetic information. During S phase in the presence of agents that cause replication stress, ATR-dependent checkpoints regulate origin firing and the replication machinery as well as prevent untimely mitosis. Here, we investigate the role of ATR during unperturbed growth in vertebrate cells. In the absence of ATR, individual replication forks progress more slowly, and an increased number of replication origins are activated. These cells also enter mitosis early and divide more rapidly, culminating in chromosome bridges and laggards at anaphase, failed cytokinesis, and cell death. Interestingly, cell death can be rescued by prolonging mitosis with partial inhibition of the mitotic cyclin-dependent kinase 1. Our data indicate that one of the essential roles of ATR during normal growth is to minimize the level of unreplicated DNA before the onset of mitosis.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据